BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,141,556	G→T	5.9%	A144S (GCC→TCC)	PP_2757 →	sugar‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,141,556	0	G	T	5.9%	324.7 / 5.3	101	A144S (GCC→TCC)	PP_2757	sugar‑binding protein
Reads supporting (aligned to +/- strand): ref base G (44/51); new base T (3/3); total (47/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 104 total aligned reads displayed.

TAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCT  >  NC_002947/3141407‑3141704

tAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTgg                                                                                                                                                      <  1:439052/150‑1 (MQ=255)

      aGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGACGTTGTCCGag                                                                                                                                                 <  1:1390532/150‑2 (MQ=255)

       gACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGCCAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACa                                                                                                                                               >  1:1057771/1‑150 (MQ=255)

       gACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACa                                                                                                                                               >  1:1302378/1‑150 (MQ=255)

        aCCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAACAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAg                                                                                                                                              >  1:1377381/1‑150 (MQ=255)

         cccTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGc                                                                                                                                             <  1:2306920/150‑1 (MQ=255)

          ncTCGGTACCCTGAGCGTGATCGACCCCNGGCTCAAGNGCATCCAGGNAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGCCAACGTCGCGGCCGGCCGCG‑CGGTGGCCGAACAGCt                                                                                                                                            >  1:643887/2‑150 (MQ=255)

          ccccGGTACTCTCAGCTTGAGTGTCCCCTGGCTCAATCGCTCCCAGGCCGCAGGGAGCCCTCTTTTCACCGTTGACGGGCCATCGCAATACAGTCTCAATAGCGCCACCACGCACAACGCCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCt                                                                                                                                            <  2:643887/147‑1 (MQ=255)

          ccTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCt                                                                                                                                            <  2:643278/150‑1 (MQ=255)

          ccTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCt                                                                                                                                            >  2:421658/1‑150 (MQ=255)

           cTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTg                                                                                                                                           >  1:1167746/1‑150 (MQ=255)

           cTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTg                                                                                                                                           <  2:775835/150‑1 (MQ=255)

            tCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACAATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTgg                                                                                                                                          >  1:233676/1‑150 (MQ=255)

               gTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTgc                                                                                                                                       <  1:828251/150‑1 (MQ=255)

                     tGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACCACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGaca                                                                                                                                 <  2:1545720/150‑1 (MQ=255)

                      gAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGacac                                                                                                                                <  1:1438811/150‑1 (MQ=255)

                       aGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACAcc                                                                                                                               >  1:425712/1‑150 (MQ=255)

                            gATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACcggcgg                                                                                                                          >  2:511180/1‑150 (MQ=255)

                               cGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCaa                                                                                                                       <  2:856995/150‑1 (MQ=255)

                                 cccccTGGCCCAATCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAgg                                                                                                                     <  2:1377381/149‑1 (MQ=255)

                                 aCCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAgg                                                                                                                     >  1:1994086/1‑150 (MQ=255)

                                    ccTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGcc                                                                                                                  >  2:2277996/1‑150 (MQ=255)

                                    ccTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGAAAGGGcc                                                                                                                  <  1:1823020/150‑1 (MQ=255)

                                       ggCTCAAGCGCATCCAGGCAACAGGTATTCCGCGTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCa                                                                                                               <  1:1931751/150‑1 (MQ=255)

                                        gCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCaa                                                                                                                       <  1:947939/141‑1 (MQ=255)

                                        gCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCaa                                                                                                                       >  2:947939/1‑141 (MQ=255)

                                          tCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATcc                                                                                                            >  2:1597675/1‑150 (MQ=255)

                                          tCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATcc                                                                                                            >  2:1743205/1‑150 (MQ=255)

                                              gcgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGgt                                                                                                        <  2:1994086/150‑1 (MQ=255)

                                                gcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGgt                                                                                                        >  1:2151416/1‑148 (MQ=255)

                                                gcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGgt                                                                                                        <  2:2151416/148‑1 (MQ=255)

                                                  aTCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTc                                                                                                    >  1:344562/1‑150 (MQ=255)

                                                  aTCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTc                                                                                                    >  1:536504/1‑150 (MQ=255)

                                                    ccAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACACCACCACCTCGGACAACGTGGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCaa                                                                                                  <  2:91355/150‑1 (MQ=255)

                                                      aGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACg                                                                                                >  2:488859/1‑150 (MQ=255)

                                                             agGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGaca                                                                                                                                 <  1:2251845/110‑1 (MQ=255)

                                                             agGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGaca                                                                                                                                 >  2:2251845/1‑110 (MQ=255)

                                                                  tCCCGCTTTTCACCATTGATGTGCCATCTCTGTACAGCCTCAACAACCCCACCTCGGACAACGTCGCGGCCGGCCGCG‑AGTTGGCCGAACAGATGCCACTGTTTACCTTCAGCAAATGCCGCATCCTGGTGTTCAACGGCTTCTACgggg                                                                                    <  1:421658/150‑1 (MQ=255)

                                                                     cGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGc                                                                                 >  2:1572911/1‑150 (MQ=255)

                                                                     cGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGc                                                                                 >  2:1972224/1‑150 (MQ=255)

                                                                     cGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGc                                                                                 >  2:1223935/1‑150 (MQ=255)

                                                                      gCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGcc                                                                                >  1:1857441/1‑150 (MQ=255)

                                                                       cTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCg                                                                               <  2:895364/150‑1 (MQ=255)

                                                                             aCCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTg                                                                                                                                           >  1:1681647/1‑84 (MQ=255)

                                                                             aCCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTg                                                                                                                                           <  2:1681647/84‑1 (MQ=255)

                                                                              ccATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCTCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgtg                                                                        <  1:531159/150‑1 (MQ=255)

                                                                              ccATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgtg                                                                        <  2:1167746/150‑1 (MQ=255)

                                                                               cATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGc                                                                       >  1:1864060/1‑150 (MQ=255)

                                                                                aTTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTATGCg                                                                      >  1:353409/1‑150 (MQ=255)

                                                                                 ttGATGTGCCATCGCAGTACAGCCTCAACAACACGACNTCGGACAACNGCTAGGCAGGGCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGAGCGCATCCTGGTGTTGAACGGCTTCTCCGGGGCGCCGGTGTGTGCGa                                                                     >  1:1036320/1‑150 (MQ=255)

                                                                                    aTGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCACG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATcc                                                                  >  2:2290952/1‑150 (MQ=255)

                                                                                     tgtgCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCg                                                                 <  1:488859/150‑1 (MQ=255)

                                                                                        gCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCtc                                                              >  1:450805/1‑149 (MQ=255)

                                                                                           aTCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACgg                                                                                      >  1:424454/1‑123 (MQ=255)

                                                                                           aTCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACgg                                                                                      <  2:424454/123‑1 (MQ=255)

                                                                                           aTCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGAGGTGCCGGTCTGTGCGATCCGCTacga                                                           >  1:243438/1‑150 (MQ=255)

                                                                                            tCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGa                                                                     <  2:320882/139‑1 (MQ=255)

                                                                                            tCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGa                                                                     >  1:320882/1‑139 (MQ=255)

                                                                                            tCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTacgac                                                          <  1:1223935/150‑1 (MQ=255)

                                                                                             cGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTacgacg                                                         <  2:1328361/150‑1 (MQ=255)

                                                                                              gCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTAcgacga                                                        <  2:344562/150‑1 (MQ=255)

                                                                                              gCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTAcgacga                                                        <  1:2277996/150‑1 (MQ=255)

                                                                                               cAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGTCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAg                                                       <  1:2300867/150‑1 (MQ=255)

                                                                                                 gTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGtt                                                     <  2:536504/150‑1 (MQ=255)

                                                                                                 gTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGtt                                                     <  2:425712/150‑1 (MQ=255)

                                                                                                    cAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCACG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTATGCTATCCTCTACGACGAGTTgaa                                                  <  2:150388/150‑1 (MQ=255)

                                                                                                      gCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaaga                                                >  2:1063100/1‑150 (MQ=255)

                                                                                                       ccTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaagaa                                               <  1:1572911/150‑1 (MQ=255)

                                                                                                        cTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGaagaag                                              <  2:1363805/150‑1 (MQ=255)

                                                                                                         tacacaccaCCACCTCGGACTCAGTCGCAGCCGAACGTG‑CGTTGGACGTACAGGTGGTTCTCTACGCCGGCGGCAAGGGCCGCTTCCTGGTATTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCGACGACGATTTTAAGAAgg                                             <  2:233676/147‑1 (MQ=255)

                                                                                                         tCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTggc                                                                                                        >  2:686403/1‑90 (MQ=255)

                                                                                                         tCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACAACGGCGGCAAGGGCCGCATCCTggc                                                                                                        <  1:686403/91‑2 (MQ=255)

                                                                                                           aacaacACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTa                                           <  2:1302378/150‑1 (MQ=255)

                                                                                                              aacaCCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTg                                                                                  >  1:2324900/1‑108 (MQ=255)

                                                                                                              aacaCCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTg                                                                                  <  2:2324900/108‑1 (MQ=255)

                                                                                                                 accaccTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGaagaag                                              <  2:1064627/141‑1 (MQ=255)

                                                                                                                 accaccTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGaagaag                                              >  1:1064627/1‑141 (MQ=255)

                                                                                                                   caccTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGa                                   <  1:1743205/150‑1 (MQ=255)

                                                                                                                     ccTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatc                                 <  2:1857441/150‑1 (MQ=255)

                                                                                                                      ttCGGCCAACG‑CGCGGCCGGCCGCGACGTTGTCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatca                                >  1:1211468/2‑150 (MQ=39)

                                                                                                                      cTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCt                                                                                          >  1:1425437/1‑92 (MQ=255)

                                                                                                                      cTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCt                                                                                          <  2:1425437/92‑1 (MQ=255)

                                                                                                                      cTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTgg                                       <  2:2199020/143‑1 (MQ=255)

                                                                                                                      cTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTgg                                       >  1:2199020/1‑143 (MQ=255)

                                                                                                                       tCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgtg                                                                        >  1:193101/1‑109 (MQ=255)

                                                                                                                       tCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgtg                                                                        <  2:193101/109‑1 (MQ=255)

                                                                                                                       tCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatcat                               >  2:2198270/1‑150 (MQ=255)

                                                                                                                        cGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatcatc                              <  1:1063100/150‑1 (MQ=255)

                                                                                                                          gACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCg                            <  2:353409/150‑1 (MQ=255)

                                                                                                                               acgcgcgGCCGGCCGCGACGTTGTCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAAt                        <  2:1211468/147‑1 (MQ=255)

                                                                                                                                gTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGc                                                                <  2:1824586/108‑1 (MQ=255)

                                                                                                                                gTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGc                                                                >  1:1824586/1‑108 (MQ=255)

                                                                                                                                cgcgcgGCCGGCCGCGACGTTGTCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCt                                                               <  2:1524760/108‑1 (MQ=255)

                                                                                                                                cgcgcgGCCGGCCGCGACGTTGTCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCt                                                               >  1:1524760/3‑110 (MQ=255)

                                                                                                                                cgcgcgGCCGGCCGCGACGTTGTCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAAtt                       >  1:1463913/3‑150 (MQ=255)

                                                                                                                                   gcgGCCGGCCACG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAg                   <  1:2290952/150‑1 (MQ=255)

                                                                                                                                       ccggccgCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGGGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGACGATCATCCGCAATTGCAGATCg               >  1:1731647/1‑150 (MQ=255)

                                                                                                                                        cggccgCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGt              <  1:1597675/150‑1 (MQ=255)

                                                                                                                                                  gtgggCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGa     <  2:1057771/147‑1 (MQ=255)

                                                                                                                                                     ggCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCt  <  1:951031/150‑1 (MQ=255)

TAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCCGGAGCT  >  NC_002947/3141407‑3141704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`