Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,294,395 G→T 6.5% L1212L (CTG→CTT)  PP_2893 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,294,3950GT 6.5% 334.0 / 7.9 107L1212L (CTG→CTT) PP_2893hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (42/58);  new base T (2/5);  total (44/63)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01

Only 100 of 111 total aligned reads displayed.

TCTGGCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGT  >  NC_002947/3294246‑3294540
                                                                                                                                                     |                                                                                                                                                 
tCTGGCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGTGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTg                                                                                                                                                   <  2:1017921/150‑1 (MQ=255)
 cTGGCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTgg                                                                                                                                                  >  1:1125169/1‑150 (MQ=255)
  tGGCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGc                                                                                                                                                 <  2:388390/150‑1 (MQ=255)
    gCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCaa                                                                                                                                               >  1:2004863/1‑150 (MQ=255)
    gCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCGGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCaa                                                                                                                                               <  2:293991/150‑1 (MQ=255)
     taaCAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACTATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAg                                                                                                                                              <  1:224739/149‑1 (MQ=255)
       aCAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCt                                                                                                                                            <  2:1031514/150‑1 (MQ=255)
        cAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGccc                                                                                                                                           >  2:2221047/1‑148 (MQ=255)
          gCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCAc                                                                                                                                         >  1:894738/1‑150 (MQ=255)
          gCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCAc                                                                                                                                         >  1:1427434/1‑150 (MQ=255)
           cACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACt                                                                                                                                        >  1:1338920/1‑150 (MQ=255)
              cAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGTTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGCCCACTggc                                                                                                                                     <  1:563007/150‑2 (MQ=255)
              cAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGCCCACTggc                                                                                                                                     <  1:2221047/150‑2 (MQ=255)
               aGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGAc                                                                                                                                    <  1:1454272/150‑1 (MQ=255)
               aGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGAc                                                                                                                                    <  1:1096366/150‑1 (MQ=255)
               aGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGCCCACTggcg                                                                                                                                    <  1:740460/150‑3 (MQ=255)
                 cGTGGTCGCCAGCGGTAATATGATCGGGAAGCTCAGCCTTCGTTACGGGGTGGGCGGGTTCGAACCGGCTAACATGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGCCTACCGCGAGGCGGCCAGTGAGGTTGCCAGCCCACTGGaggc                                                                                                                                  <  2:183997/150‑4 (MQ=255)
                   tGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAg                                                                                                                                              >  1:724011/1‑136 (MQ=255)
                   tGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAg                                                                                                                                              <  2:724011/136‑1 (MQ=255)
                     gTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAtcttc                                                                                                                              <  2:2258133/150‑1 (MQ=255)
                      tCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCCACACGATCGCGTTGCGTTTCAAGCTGAGCAAGAACGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAtcttct                                                                                                                             <  1:2188185/150‑1 (MQ=255)
                      tCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAtcttct                                                                                                                             >  2:2349135/1‑150 (MQ=255)
                      tCGCCAGCGGTAACATCACCGAGAAGCTCAGCCCCCCCTACCCGGCGGGCGCCCCCCCACCCCCCAACACCACCCCCCCGCGCTCCAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAtcttct                                                                                                                             >  1:318296/1‑150 (MQ=255)
                       cGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTa                                                                                                                            <  1:1225614/150‑1 (MQ=255)
                       cGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGAGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTa                                                                                                                            <  2:1109926/150‑1 (MQ=255)
                          cAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACaa                                                                                                                         >  1:322592/1‑150 (MQ=255)
                          cAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGATGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAAGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTTGACATCTTCTACaa                                                                                                                         >  1:2299488/1‑150 (MQ=255)
                            gCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAgc                                                                                                                       <  1:1465232/150‑1 (MQ=255)
                               gTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGAc                                                                                                                                    <  2:1958957/134‑1 (MQ=255)
                               gTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGAc                                                                                                                                    >  1:1958957/1‑134 (MQ=255)
                                tAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGATCCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGa                                                                                                                   <  2:1612356/150‑1 (MQ=255)
                                    aTCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTc                                                                                                               <  2:1125169/150‑1 (MQ=255)
                                     tCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAATCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCt                                                                                                              <  1:2349135/150‑1 (MQ=255)
                                     tCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCt                                                                                                              >  2:1866207/1‑150 (MQ=255)
                                      cACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTa                                                                                                             <  2:2004863/150‑1 (MQ=255)
                                       aCCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAgcgc                                                                                                                     >  1:407688/1‑141 (MQ=255)
                                       aCCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAg                                                                                                            <  2:318296/150‑1 (MQ=255)
                                        ccGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAgg                                                                                                           >  1:1075630/1‑150 (MQ=255)
                                         cGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTGGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGc                                                                                                          >  1:586323/1‑150 (MQ=255)
                                                 tCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAAGACGATCGCGTTTCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCg                                                                                                  >  1:2296023/1‑150 (MQ=255)
                                                 tCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCg                                                                                                  >  2:504645/1‑150 (MQ=255)
                                                       ttCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcgg                                                                                            >  2:925505/1‑150 (MQ=255)
                                                        tCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcggc                                                                                           >  1:167551/1‑150 (MQ=255)
                                                        tCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcggc                                                                                           <  2:1075630/150‑1 (MQ=255)
                                                               ggggTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAac                                                                                    <  1:1163878/150‑1 (MQ=255)
                                                                  gTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAacgga                                                                                 >  2:841654/1‑150 (MQ=255)
                                                                  gTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAacgga                                                                                 <  2:1427434/150‑1 (MQ=255)
                                                                     ggCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGc                                                                                                                                             >  1:2322673/1‑87 (MQ=255)
                                                                     ggCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGc                                                                                                                                             <  2:2322673/87‑1 (MQ=255)
                                                                     ggCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACtt                                                                                                                >  2:2228729/1‑116 (MQ=255)
                                                                     ggCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACtt                                                                                                                <  1:2228729/116‑1 (MQ=255)
                                                                       cGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTgg                                                                                                                                                  <  1:1474218/80‑1 (MQ=255)
                                                                       cGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTgg                                                                                                                                                  >  2:1474218/1‑80 (MQ=255)
                                                                        gtgtTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACCACTATGCCCGCAGCGTGGCGGCCATCAACGGACGGTgg                                                                           >  1:1330200/1‑150 (MQ=255)
                                                                         tgtTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGgc                                                                          <  1:504645/150‑1 (MQ=255)
                                                                          gtTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGgcc                                                                         <  2:586323/150‑1 (MQ=255)
                                                                           ttCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGgccg                                                                        >  2:1608370/1‑150 (MQ=255)
                                                                              gAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCaa                                                                                                                                               >  2:700227/1‑76 (MQ=255)
                                                                              gAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCaa                                                                                                                                               <  1:700227/76‑1 (MQ=255)
                                                                                 ccGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                  <  2:167551/150‑1 (MQ=255)
                                                                                 ccGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                  <  2:322592/150‑1 (MQ=255)
                                                                                    gCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTc                                                                                                               >  1:1490754/1‑102 (MQ=255)
                                                                                    gCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCt                                                                                                              <  2:2066693/103‑1 (MQ=255)
                                                                                    gCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCt                                                                                                              >  1:2066693/1‑103 (MQ=255)
                                                                                     ccAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCAt                                                                                        >  2:326424/1‑124 (MQ=255)
                                                                                     ccAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCAt                                                                                        <  1:326424/124‑1 (MQ=255)
                                                                                      cAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAAc                                                             <  1:1996677/150‑1 (MQ=255)
                                                                                       aaCACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGGGGCCATCAACGGACGGTGGCCGCCTCGATGAACt                                                            >  1:1431086/1‑150 (MQ=255)
                                                                                       aaCACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACt                                                            >  2:1980391/1‑150 (MQ=255)
                                                                                       aaCACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACCTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACt                                                            >  1:2284997/1‑150 (MQ=255)
                                                                                            gATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTTGCGGCCCTCAACGGACGGTGGACGCCTCGATGAACTCGTTg                                                       >  2:466912/1‑150 (MQ=255)
                                                                                            gATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTg                                                       >  1:1513657/1‑150 (MQ=255)
                                                                                            gATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTg                                                       >  1:2066584/1‑150 (MQ=255)
                                                                                             aTCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTgg                                                                                               <  2:138745/109‑1 (MQ=255)
                                                                                                  gttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCg                                                 <  1:925505/150‑1 (MQ=255)
                                                                                                        ttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAg                                           <  1:1018541/150‑1 (MQ=255)
                                                                                                        ttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAg                                           >  1:1417925/1‑150 (MQ=255)
                                                                                                          aCAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGtt                                                        >  1:905923/1‑135 (MQ=255)
                                                                                                          aCAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGtt                                                        <  2:905923/135‑1 (MQ=255)
                                                                                                            aaGCTGAGCAAGAAAGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTTGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCg                                       <  2:2299488/150‑1 (MQ=255)
                                                                                                              gCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAac                                                                                    <  2:1958027/103‑1 (MQ=255)
                                                                                                              gCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAac                                                                                    >  1:1958027/1‑103 (MQ=255)
                                                                                                                tGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtgg                                   <  2:1662594/150‑1 (MQ=255)
                                                                                                                  aGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtgggg                                 <  2:2066584/150‑1 (MQ=255)
                                                                                                                  aGCAAGAAGCTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTAGATGAACTCGTTGGTGTCGGGTGAGGGCagtgggg                                 <  1:466912/150‑1 (MQ=255)
                                                                                                                   gCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggt                                <  1:20504/150‑1 (MQ=255)
                                                                                                                      agaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGggtggt                             <  1:1350460/150‑1 (MQ=255)
                                                                                                                       gaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATg                                                                >  1:1494783/1‑114 (MQ=255)
                                                                                                                       gaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATg                                                                <  2:1494783/114‑1 (MQ=255)
                                                                                                                        aagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGtggtgg                           <  1:841654/150‑1 (MQ=255)
                                                                                                                               aCCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAgcgc                                                                                                                     >  2:398784/1‑53 (MQ=255)
                                                                                                                               aCCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAgcgc                                                                                                                     <  1:398784/53‑1 (MQ=255)
                                                                                                                                ccTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTAcc                   >  2:2172724/1‑150 (MQ=255)
                                                                                                                                 cTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                  <  2:573110/102‑1 (MQ=255)
                                                                                                                                           ccAGTGAGCTTGCCAGCCCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                  >  2:2176664/1‑92 (MQ=38)
                                                                                                                                           ccAGTGAGCTTGCCAGCCCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                  <  1:2176664/92‑1 (MQ=39)
                                                                                                                                            cAGTGGGCTGTCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAgg       <  2:1338920/150‑1 (MQ=255)
                                                                                                                                                 ggCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGgt  <  1:1866207/150‑1 (MQ=255)
                                                                                                                                                 ggCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGgt  <  1:629977/150‑1 (MQ=255)
                                                                                                                                                 ggCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGCTGAGGGCGTTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGgt  <  1:1162528/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TCTGGCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGT  >  NC_002947/3294246‑3294540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑