| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 308,741 | A→G | 8.9% | intergenic (+1755/‑115) | hslO → / → PP_0254 | molecular chaperone HslO/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 308,741 | 0 | A | G | 8.9% | 271.7 / 13.7 | 102 | intergenic (+1755/‑115) | hslO/PP_0254 | molecular chaperone HslO/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (45/47); new base G (7/2); total (53/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
TGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTTCTCC > NC_002947/308596‑308888 | tGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAgggg > 1:317652/1‑150 (MQ=255)tGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAAGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAgggg > 2:1378009/1‑150 (MQ=255)tGACAAAGAGGCATAAGAGGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAgggg > 2:1792150/1‑150 (MQ=255) aCAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGCAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGggcg > 2:81922/1‑150 (MQ=255) aCAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGggcg > 1:1018556/1‑150 (MQ=255) aCAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGggcg > 2:1205797/1‑150 (MQ=255) aaaGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGTGAAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGggcggc > 1:1832837/1‑150 (MQ=255) aGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑tt < 1:136658/150‑1 (MQ=255) aGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑tt > 2:1701959/1‑150 (MQ=255) aGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑tt > 2:1701808/1‑150 (MQ=255) gCCTACGACGAGGCTGCCAAAGGTCTGGCCCAGCTGTACATGGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑tttttt < 2:2372588/150‑1 (MQ=255) gCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAggg < 2:127872/139‑1 (MQ=255) gCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑tttttt < 1:282700/150‑1 (MQ=255) gCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAACCGCAACGAGAAAGCCGCCCCCAggg > 1:127872/1‑139 (MQ=255) ccTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTTTGCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTt‑g > 1:2372588/1‑150 (MQ=255) gacgaGGCTGCAAAAGGTATGGCCACGATGTTCATCAAAAACTTCTAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGt < 2:1832837/150‑1 (MQ=255) cggCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAgg < 1:1792150/149‑1 (MQ=255) aGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAgg < 2:326764/150‑1 (MQ=255) gCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGc > 2:1982429/1‑150 (MQ=255) tGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCtt > 1:365752/1‑150 (MQ=255) tGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCtt > 1:344068/1‑150 (MQ=255) aaaGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGccc > 1:2266628/1‑150 (MQ=255) aaaGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGccc > 1:315322/1‑150 (MQ=255) aaaGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCCCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGccc > 1:61309/1‑150 (MQ=255) gTCTGGCCAAGCTGTTCGTCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGa > 2:435149/1‑150 (MQ=255) gTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGa > 2:1011136/1‑150 (MQ=255) tCTGGCCAAGCTGTTCGTCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGaa < 1:435149/150‑1 (MQ=255) cTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAg > 1:2231852/1‑150 (MQ=255) tGGCCAAGCTGTTCATCGACAACGTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAgg < 1:248309/150‑1 (MQ=255) tGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAgg < 1:653092/150‑1 (MQ=255) cAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTt‑gt < 2:2295269/123‑1 (MQ=255) cAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTt‑gt > 1:2295269/1‑123 (MQ=255) cAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGcc > 2:11076/1‑150 (MQ=255) aaGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGccc < 2:2258971/150‑1 (MQ=255) gCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGc < 1:1701959/150‑1 (MQ=255) gCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGc < 1:1701808/150‑1 (MQ=255) aTCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCa < 1:81922/150‑1 (MQ=255) aTCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCa < 1:49744/150‑1 (MQ=255) gAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTTAGTGGGTTAGGGCTTCCCCGCGAAGGGGCCGGCATAGGCAgg > 2:1644211/1‑149 (MQ=255) aaaCTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATa < 1:1606879/141‑1 (MQ=255) aaaCTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATa > 2:1606879/1‑141 (MQ=255) ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCACCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTc > 2:1407506/1‑150 (MQ=255) tCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGAGGTCCCGCAGCTGTAAGCCGAGCTCAGCCGCAAGGAGACACGAGAAAGGCTTCTCGCT‑GGGGTG‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCt > 1:550997/1‑150 (MQ=255) aagaagTTCGAAGTTCCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTgg < 2:1679117/150‑1 (MQ=255) agaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCCGCCAAAggacggg > 2:1942520/1‑144 (MQ=255) gaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGtt > 2:2253242/1‑150 (MQ=255) gTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttttt < 2:344068/150‑1 (MQ=255) ttCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTc < 2:1521518/150‑1 (MQ=255) gAAGTTTCCGACACCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAg < 1:1764062/99‑1 (MQ=255) gAAGTTTCCGACACCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAg > 2:1764062/1‑99 (MQ=255) aGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGttt < 1:2253242/150‑1 (MQ=255) gTTTCCGACGCCAACCAGGCCGCCGGCCCGCAGCTGTAAGCCGAGCTCAGCCGGAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGttt < 1:1644211/150‑1 (MQ=255) tttCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTg > 1:843813/1‑145 (MQ=255) tttCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTg < 2:843813/145‑1 (MQ=255) tttCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCa < 2:365752/150‑1 (MQ=255) cGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGt < 1:1942520/150‑1 (MQ=255) gACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTc > 1:2258278/1‑150 (MQ=255) gACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTACGTCAAGCCGAAGAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTCGGGCTTTCCCTCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCagatcg > 1:1642408/1‑145 (MQ=255) aCGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCa < 2:315322/150‑1 (MQ=255) cATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCAt > 1:1745278/1‑150 (MQ=255) cATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCCCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCAt < 2:61309/150‑1 (MQ=255) aaGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCAt > 2:384158/1‑150 (MQ=255) gCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGtt > 1:1659661/1‑130 (MQ=255) gCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGtt < 2:1659661/130‑1 (MQ=255) gCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGTATACGCAGCGAAAGTCTGGTTTTTCTGTGTCACTGTCAGCATCCATTGCGaa < 1:1378009/150‑1 (MQ=255) gatcccGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTGGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGa < 1:168429/147‑1 (MQ=255) cccGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACaa < 2:695040/150‑1 (MQ=255) cGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAaga < 1:425963/150‑1 (MQ=255) cGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAaga < 1:1011136/150‑1 (MQ=255) gCAGCTGTAAGCCGAGCTCAGCCGCAACGACCAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GCGGGTTAAGGCTTGCCCGCGAAGGAGCCCGCATAGGGAGCGAAAGACTGGTTTTTCTGTTTCACTGTCAGCATACATTGCGAAGACagatc > 2:168429/1‑146 (MQ=255) aGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagcc > 1:2254967/1‑150 (MQ=255) gCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagcca < 1:11076/150‑1 (MQ=255) gTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagccagcc < 2:2254967/150‑1 (MQ=255) aGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAAgcagccagt > 2:942119/1‑149 (MQ=255) gCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGNC‑TTTTTN‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCGAGCCAGag > 1:1703348/1‑149 (MQ=255) gCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGAt < 2:550997/150‑1 (MQ=255) cGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGa < 1:1205797/150‑1 (MQ=255) cgCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGAcc < 1:942119/149‑1 (MQ=255) tCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagcc < 1:1570539/135‑1 (MQ=255) tCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagcc > 2:1570539/1‑135 (MQ=255) ccGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑GTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAAAGCCAGCCAGATGACCGAAGccc > 2:120455/1‑150 (MQ=255) aaCGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCa < 2:1018556/150‑1 (MQ=255) aCGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAg < 1:384158/150‑1 (MQ=255) cGAGAAAGCCGCCCCCAGGCGCGGC‑TTTTGT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCTAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAgc < 2:1703348/150‑1 (MQ=255) aaaaaGCCGCCCCTGGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt < 1:2068268/74‑1 (MQ=38) aaaaaGCCGCCCCTGGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt > 2:2068268/3‑76 (MQ=38) aaGCCGCCCCTGGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGGTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGCGCCAGCCAGATGACCGAAGCCCCTCAGCGCaat > 1:253534/1‑148 (MQ=255) aaGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACt < 2:2258278/150‑1 (MQ=255) aGCCGCCCCTGGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTc < 2:665521/82‑1 (MQ=255) aGCCGCCCCTGGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTc > 1:665521/1‑82 (MQ=255) gccgccCCTGGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGc > 2:293410/1‑150 (MQ=255) gccgccCCTGGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGc > 2:2292892/1‑150 (MQ=255) gccgccCCTGGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGc > 1:1130562/1‑150 (MQ=255) cgccCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGtt > 2:1037166/1‑83 (MQ=255) cgccCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGtt < 1:1037166/83‑1 (MQ=255) ccccAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTtct < 2:317652/150‑1 (MQ=255) cccAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTtctc > 2:42633/1‑150 (MQ=255) cccAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTtc < 1:2188371/148‑1 (MQ=255) cccAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTtc > 2:2188371/1‑148 (MQ=255) ccAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAATAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTTCTcc > 2:103424/1‑150 (MQ=255) | TGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGC‑TTTTTT‑GTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTTCTCC > NC_002947/308596‑308888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |