Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,489,710 A→C 5.7% L575R (CTG→CGG)  clpV ← protein ClpV1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,489,7100AC 5.7% 283.0 / 6.0 98L575R (CTG→CGG) clpVprotein ClpV1
Reads supporting (aligned to +/- strand):  ref base A (44/48);  new base C (2/4);  total (46/52)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TCTTGCCGACGCCGCTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGCACGCGCGCCAGCTCGGCACGCAG  >  NC_002947/3489561‑3489858
                                                                                                                                                     |                                                                                                                                                    
tCTTGCCGACGCCGCTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCa                                                                                                                                                      <  1:750395/150‑1 (MQ=255)
 cTTGCCGACGCCGCTGGGGCCGAGCAGCAGGAACACGTCAATCGGTTTGTTCGGGTCTTCCATGAGAATGAAGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAg                                                                                                                                                     <  1:419690/150‑1 (MQ=255)
 cTTGCCGACGCCGCTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAg                                                                                                                                                     <  2:862341/150‑1 (MQ=255)
      cGACGCCGCTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCt                                                                                                                                                >  1:1661853/1‑150 (MQ=255)
           ccgcTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACg                                                                                                                                           <  1:1158929/150‑1 (MQ=255)
           ccgcTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACg                                                                                                                                           <  2:1260576/150‑1 (MQ=255)
           ccgcTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGCGCCTGCCGGCCCTGcttg                                                                                                                                           <  1:2221533/150‑4 (MQ=255)
           ccgcTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGAGTGTTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACg                                                                                                                                           <  1:2243662/150‑1 (MQ=255)
            cgcTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCCCCAAGCAGGGCCGGCAGGCGCTGCACgg                                                                                                                                          >  2:858735/1‑150 (MQ=255)
            cgcTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACgg                                                                                                                                          <  2:345498/150‑1 (MQ=255)
              cTGGGGCCGAGCAGCAGGAACACGCGAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGAGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTa                                                                                                                                        <  2:237002/150‑1 (MQ=255)
              cTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTa                                                                                                                                        <  2:830542/150‑1 (MQ=255)
               tGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTAt                                                                                                                                       <  2:937885/150‑1 (MQ=255)
                ggggCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATc                                                                                                                                      <  2:848702/150‑1 (MQ=255)
                ggggCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATc                                                                                                                                      <  2:650228/150‑1 (MQ=255)
                   gacGAGCAGCAGGAACTCGCCAATCGGTTTGTTCGGTTCTTCCATGCGCGCTCGGGAAATCTTGATGCGGTTTCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGAt                                                                                                                                   <  1:1489992/148‑1 (MQ=255)
                     cGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGTAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGAttt                                                                                                                                 <  2:327570/150‑1 (MQ=255)
                     cGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGAttt                                                                                                                                 <  2:2170268/150‑1 (MQ=255)
                     cGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGCGCCTGCCGGCCCTGcttgg                                                                                                                                          <  1:2355791/141‑5 (MQ=255)
                     cGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGCGCCTGCCGGCCCTGcttgg                                                                                                                                          >  2:2355791/1‑137 (MQ=255)
                      gAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGa                                                                                                                                    >  2:581973/1‑146 (MQ=255)
                      gAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGa                                                                                                                                    <  1:581973/146‑1 (MQ=255)
                          agcagGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCa                                                                                                                            >  1:2116999/1‑150 (MQ=255)
                           gcagGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCAc                                                                                                                           <  2:250517/150‑1 (MQ=255)
                           gcagGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCAc                                                                                                                           >  2:1691162/1‑150 (MQ=255)
                             agGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACgc                                                                                                                         >  2:2288086/1‑150 (MQ=255)
                                  cacGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCGCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGGGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCAt                                                                                                                    <  1:1737363/150‑1 (MQ=255)
                                     gCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCAGGGTATCGATTTCGTCACGCAGCATCtt                                                                                                                 <  1:1684168/150‑1 (MQ=255)
                                     gCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTAGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCtt                                                                                                                 >  2:2125580/1‑150 (MQ=255)
                                      ccAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTg                                                                                                                <  1:1704889/150‑1 (MQ=255)
                                        aaTCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGcc                                                                                                              >  2:1323199/1‑150 (MQ=255)
                                         aTCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCa                                                                                                                                             <  2:42667/118‑1 (MQ=255)
                                         aTCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCa                                                                                                                                             >  1:42667/1‑118 (MQ=255)
                                             gTTTGTTCGGGTCTTCCATGCGCGCTCGGTAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCg                                                                                                         >  2:514651/1‑150 (MQ=255)
                                             gTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCa                                                                                                                            <  2:39156/131‑1 (MQ=255)
                                             gTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCa                                                                                                                            >  1:39156/1‑131 (MQ=255)
                                              tttGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCCGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCgg                                                                                                        <  1:1008152/150‑1 (MQ=255)
                                                tGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGa                                                                                                      <  2:656560/150‑1 (MQ=255)
                                                   tCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGc                                                                                                   <  1:2288086/150‑1 (MQ=255)
                                                     gggTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCg                                                                                                 <  2:1661853/150‑1 (MQ=255)
                                                       gTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGt                                                                                               >  2:1128520/1‑150 (MQ=255)
                                                        tCTTCCATGCGCGCTCGGTAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCTGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTc                                                                                              <  1:514651/150‑1 (MQ=255)
                                                             cATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGcc                                                                                         >  1:1319943/1‑150 (MQ=255)
                                                              aTGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCa                                                                                        >  1:845834/1‑150 (MQ=255)
                                                                   cgcTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACtgat                                                                                   <  2:2352519/150‑1 (MQ=255)
                                                                   cgcTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACtgat                                                                                   <  2:2116999/150‑1 (MQ=255)
                                                                   cgcTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGCGCCTGCCGGCCCTGcttg                                                                                                                                           >  1:1326900/1‑91 (MQ=255)
                                                                   cgcTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGCGCCTGCCGGCCCTGcttg                                                                                                                                           <  2:1326900/94‑4 (MQ=255)
                                                                        gggAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGCTGCCGGTCCAGCCACTGATGACCt                                                                              >  2:364973/1‑150 (MQ=255)
                                                                          gAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTgg                                                                            >  1:184128/1‑150 (MQ=255)
                                                                          gAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTgg                                                                            >  2:617888/1‑150 (MQ=255)
                                                                            aaTCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGc                                                                          <  1:221400/150‑1 (MQ=255)
                                                                            aaTCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGc                                                                          <  1:279375/150‑1 (MQ=255)
                                                                               cTTGATGCGCTTGCCGATCTCATGCAGGGCGTAGTCCTGGAACCGTCAGCGCTCCCTCCGCACGGCCGGCCGGCGCTGCACCGTATAGATTTCCTCACGCATCATCGTGCCCAGCGGGATGCCGGGCCAGCCACTGATGACCTGGGCggt                                                                       <  2:2078694/150‑3 (MQ=255)
                                                                                ttGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATg                                                                      <  2:2265886/150‑1 (MQ=255)
                                                                                    tGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGgcgc                                                                  >  1:330827/1‑150 (MQ=255)
                                                                                       gcTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGcgccgc                                                               >  1:385803/1‑150 (MQ=255)
                                                                                         ttGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTg                                                             >  1:1443414/1‑150 (MQ=255)
                                                                                         ttGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTg                                                             >  1:819062/1‑150 (MQ=255)
                                                                                         ttGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGAGCCCCAGGGGTTGTTTTTCTTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTTATGACCTGGGCGATGGCGCCGCTg                                                             >  2:695219/1‑150 (MQ=255)
                                                                                          tGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGt                                                            <  1:2027329/150‑1 (MQ=255)
                                                                                           gCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTc                                                           <  2:1319943/150‑1 (MQ=255)
                                                                                             cGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCAc                                                                                       >  2:1257597/1‑120 (MQ=255)
                                                                                             cGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCAc                                                                                       <  1:1257597/120‑1 (MQ=255)
                                                                                             cGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGa                                                         >  1:1199413/1‑150 (MQ=255)
                                                                                             cGATCTCACGCAGGGCGTGGGCCTGGCCCCGCAAGCGGGCACCAAGCCGGGCGGGCAGGCGCTGCACGGTATCGATTTCGGCACGCAGCATCTGGCCCAGCGGGATGCCGGGCCAGCTACTGATGACCTGGGCGATGGCGCCGCTGTCGa                                                         <  1:1057651/150‑1 (MQ=255)
                                                                                                  tcATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTgg                                                                            >  2:2041294/1‑126 (MQ=255)
                                                                                                  tcATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTgg                                                                            <  1:2041294/126‑1 (MQ=255)
                                                                                                    aTGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAgcgc                                                  >  2:204461/1‑150 (MQ=255)
                                                                                                     tGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGACGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGcgcg                                                 >  1:1953656/1‑150 (MQ=255)
                                                                                                       cAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTg                                               >  2:245625/1‑150 (MQ=255)
                                                                                                         gggCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGa                                             <  2:184128/150‑1 (MQ=255)
                                                                                                         gggCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGa                                             >  2:4748/1‑150 (MQ=255)
                                                                                                          ggCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGAc                                            <  2:845834/150‑1 (MQ=255)
                                                                                                          ggCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGAc                                            >  1:1431464/1‑150 (MQ=255)
                                                                                                                 tCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGc                                     >  2:1405885/1‑150 (MQ=255)
                                                                                                                 tCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATGTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGTGCGATGTCGCTGCTCTGAACCTGCGTGTGAACCAGAgga                                     >  2:126509/1‑149 (MQ=255)
                                                                                                                   cTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGAc                                                        >  2:1921163/1‑129 (MQ=255)
                                                                                                                   cTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGAc                                                        <  1:1921163/129‑1 (MQ=255)
                                                                                                                        ccAGCACGCGCTCACCAATCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGc                              >  1:1288451/1‑150 (MQ=255)
                                                                                                                        ccAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCga                              <  1:617888/150‑2 (MQ=255)
                                                                                                                           gCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCtt                           <  2:148261/150‑1 (MQ=255)
                                                                                                                           gCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCtt                           <  1:1691162/150‑1 (MQ=255)
                                                                                                                            cACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTg                          >  1:2355177/1‑150 (MQ=255)
                                                                                                                             aCGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGc                         >  1:288411/1‑150 (MQ=255)
                                                                                                                               gcgcTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGCAc                       <  1:1323199/150‑1 (MQ=255)
                                                                                                                               gcgcTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGATGTATTCGCTGATTACCTGGGCGATGGCGCAGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGCAc                       >  1:1621718/1‑150 (MQ=255)
                                                                                                                               gcgcTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCGTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCACCTGGACCAGTGGCTGGTCGCCTTGCAc                       >  2:759335/1‑150 (MQ=255)
                                                                                                                                 gcTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGCAcgc                     >  1:752242/1‑150 (MQ=255)
                                                                                                                                   tCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGCAcgcgc                   <  2:2288280/150‑1 (MQ=255)
                                                                                                                                     aCCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATg                                                                      <  1:62333/97‑1 (MQ=255)
                                                                                                                                     aCCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATg                                                                      >  2:62333/1‑97 (MQ=255)
                                                                                                                                     aCCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGCACgcgcgc                 >  1:1925713/1‑150 (MQ=255)
                                                                                                                                     aCCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGCACgcgcgc                 >  1:17810/1‑150 (MQ=255)
                                                                                                                                      ccAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGCACGCGCGcc                >  1:1336447/1‑150 (MQ=255)
                                                                                                                                       cAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACtga                                                                                    >  2:1059504/1‑81 (MQ=255)
                                                                                                                                       cAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACtga                                                                                    <  1:1059504/81‑1 (MQ=255)
                                                                                                                                            aGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGCACGCGCGCCAGCTCg          <  2:1288451/150‑1 (MQ=255)
                                                                                                                                                    cAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGCACGCGCGCCAGCTCGGCACGCAg  <  2:385803/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTTGCCGACGCCGCTGGGGCCGAGCAGCAGGAACACGCCAATCGGTTTGTTCGGGTCTTCCATGCGCGCTCGGGAAATCTTGATGCGCTTGCCGATCTCATGCAGGGCGTGGTCCTGGCCCAGCACGCGCTCACCAAGCAGGGCCGGCAGGCGCTGCACGGTATCGATTTCGTCACGCAGCATCTTGCCCAGCGGGATGCCGGTCCAGCCACTGATGACCTGGGCGATGGCGCCGCTGTCGACCAGCGCGTGGACCAGTGGCTGGTCGCCTTGCACGCGCGCCAGCTCGGCACGCAG  >  NC_002947/3489561‑3489858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑