BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,490,910	G→C	5.0%	P175R (CCG→CGG) ‡	clpV ←	protein ClpV1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,490,910	0	G	C	5.0%	318.3 / 6.3	100	P175R (CCG→CGG) ‡	clpV	protein ClpV1
Reads supporting (aligned to +/- strand): ref base G (47/48); new base C (2/3); total (49/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

Only 100 of 103 total aligned reads displayed.

TGCCGTACTTCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTCAAGCAGAGCCTGCAGCAGG  >  NC_002947/3490763‑3491056

tGCCGTACTTCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTCCCGCGGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGCCAGCCCAGCggc                                                                                                                                                  <  1:559407/150‑2 (MQ=255)

 gCCGTACTTCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGc                                                                                                                                                 >  2:236968/1‑150 (MQ=255)

   cGTACTTCGAACTCACGGCCCAGCACCTGTTTTATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTg                                                                                                                                               <  1:1020270/150‑1 (MQ=255)

   cGTACTTCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTg                                                                                                                                               <  2:1328989/150‑1 (MQ=255)

   cGTACTTCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGAGGCGTCCGCGAGTTTACTCATGGACTGGTCCAGCGCCGGGGTCTTGCCGCTGGACTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTg                                                                                                                                               <  1:285358/150‑1 (MQ=255)

     tACTTCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGATCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGgc                                                                                                                                             <  1:844737/150‑1 (MQ=255)

     tACTTCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGgc                                                                                                                                             <  1:1576971/150‑1 (MQ=255)

        ttCGAACTCACGGCCCAGCACCGGGTCTATGCGGCCTTCGCGGGCGCTTTGGGTAGGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGGG‑CCGGTGGGCTGGCCAGCGGACTGgcctg                                                                                                                                          >  2:2364486/1‑150 (MQ=255)

        ttCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGgcctg                                                                                                                                          >  1:1110425/1‑150 (MQ=255)

        ttCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGgcctg                                                                                                                                          >  2:1586983/1‑150 (MQ=255)

        ttCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCAGGCCAGCCCGCTGgccag                                                                                                                                          >  1:167267/1‑148 (MQ=255)

        ttCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCAGGCCAGCCCGCTGgccag                                                                                                                                          <  2:167267/150‑3 (MQ=255)

        ttCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCAGGCCAGCCCGCTGgccag                                                                                                                                          <  2:1380948/150‑3 (MQ=255)

        ttCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTACTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGgcctg                                                                                                                                          >  1:1512611/1‑150 (MQ=255)

        ttCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCGCGACGTGCTCACTGGTG‑CCGCTGGGCTGGCCCGCGGGCTGgcctc                                                                                                                                          >  2:1708558/1‑149 (MQ=255)

           gAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCGCCCGCTGGCCAGCCCAGCggcgc                                                                                                                                                >  2:1914811/1‑139 (MQ=255)

           gAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCGCCCGCTGGCCAGCCCAGCggcgc                                                                                                                                                <  1:1914811/142‑4 (MQ=255)

            aaCTCACCGCCCAGCACCGGGTCGATGCGTCCTTCGCGGGCGCTTTGGGTAAGGTTGACCTTGTACTGGTCCAGCTCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGcctgcctg                                                                                                                                      <  1:1968945/150‑1 (MQ=255)

               tCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGgcctgcccag                                                                                                                                     >  1:1458363/1‑145 (MQ=255)

               tCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCAGGCCAGCCCGCTGgccagccc                                                                                                                                       <  1:250361/146‑2 (MQ=255)

               tCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCAGGCCAGCCCGCTGgccagccc                                                                                                                                       >  2:250361/1‑145 (MQ=255)

                 aCGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTc                                                                                                                                 <  1:1428585/150‑1 (MQ=255)

                   ggCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCgg                                                                                                                               <  1:662898/150‑1 (MQ=255)

                      ccAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGgcgc                                                                                                                            <  1:1586983/150‑1 (MQ=255)

                      ccAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGgcgc                                                                                                                            >  2:1324444/1‑150 (MQ=255)

                       cAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGgcg                                                                                                                             <  2:1281092/148‑1 (MQ=255)

                       cAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGgcg                                                                                                                             >  1:1281092/1‑148 (MQ=255)

                       cAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTCTGGGTAAGGCTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGCCGTGCTCCCCGGCG‑CCGCTGCGCCGGCCCGCGGGCTGGCCTGCCCGGTCGCGgcgcc                                                                                                                           >  1:1018058/1‑149 (MQ=255)

                        aGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTa                                                                                                                          <  2:1152510/150‑1 (MQ=255)

                        aGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTa                                                                                                                          <  1:836655/150‑1 (MQ=255)

                        aGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACATGCTCACTCGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTa                                                                                                                          >  2:2244913/1‑150 (MQ=255)

                                       cGGCCTTCGCCCGCTCTTTGGCTAAGCTTGACCTTGTACTTGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTCCTCCACGTGCTCTTTCTCG‑CCGCTGGGCTGGCCAGCGGCCTGGCCTGCCTTTACTCGGCGCTACCCTCCACCAGcgcg                                                                                                           <  2:815483/150‑1 (MQ=255)

                                           cTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCaa                                                                                                       >  1:1460015/1‑150 (MQ=255)

                                             tCGCGGGCGCTTTGGGTCTCGGTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAgg                                                                                                     <  1:1328555/150‑1 (MQ=255)

                                              cgcgGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCTCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGt                                                                                                    <  2:346625/150‑1 (MQ=255)

                                                 gggCGCTTTGGGTACGGTTGACAGGGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCa                                                                                                 <  2:1758810/150‑1 (MQ=255)

                                                 gggCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCa                                                                                                 >  1:2313370/1‑150 (MQ=255)

                                                 gggCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCa                                                                                                 <  2:232607/150‑1 (MQ=255)

                                                       tttGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCCGGCGCa                                                                                           >  2:538640/1‑150 (MQ=255)

                                                       tttGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCa                                                                                           >  1:1478079/1‑150 (MQ=255)

                                                       tttGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCa                                                                                           <  1:1426240/150‑1 (MQ=255)

                                                         tGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGACGATGGAATTGCGCGAGGTGCGCACTGGCA‑CACGTGTGCTGGCCAGCGGGCTTGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAgg                                                                                         <  1:1741155/150‑1 (MQ=255)

                                                             tAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGgcgc                                                                                                                            >  1:1238335/1‑111 (MQ=255)

                                                             tAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGgcgc                                                                                                                            <  2:1238335/111‑1 (MQ=255)

                                                             tAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCAt                                                                                     <  2:2025524/150‑1 (MQ=255)

                                                              aaGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATc                                                                                    <  1:951967/150‑1 (MQ=255)

                                                                 gTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAg                                                                                                >  1:1367953/1‑135 (MQ=255)

                                                                 gTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAg                                                                                                <  2:1367953/135‑1 (MQ=255)

                                                                    gACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGtt                                                                              >  2:1859590/1‑150 (MQ=255)

                                                                      ccGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGa                                                                            <  1:333477/150‑1 (MQ=255)

                                                                       cGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGAt                                                                           >  1:339286/1‑150 (MQ=255)

                                                                        gtgtACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGAtt                                                                          >  2:1402228/1‑150 (MQ=255)

                                                                             cTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTc                                                                     <  1:236968/150‑1 (MQ=255)

                                                                             cTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTc                                                                     <  1:1423559/150‑1 (MQ=255)

                                                                                gTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAg                                                                  >  1:1821614/1‑150 (MQ=255)

                                                                                     gcgcCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCg                                                             <  2:1302446/150‑1 (MQ=255)

                                                                                      cgcCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCgg                                                            >  1:1935511/1‑150 (MQ=255)

                                                                                       gcCGGGGTCTTGCCGCTGGCCTTGCTCGACTTGCTCACTGGCG‑CCGCTGGGCTGGCCAGAGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCTCGGCAAGGTTCAGGCGCAGTTCATCGGCGTTGATTTTTTCCAGCTCCGGt                                                           <  2:811415/150‑1 (MQ=255)

                                                                                       gcCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGt                                                           >  1:72728/1‑150 (MQ=255)

                                                                                         cGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGc                                                         >  2:66925/1‑150 (MQ=255)

                                                                                         cGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGc                                                         >  1:1695976/1‑150 (MQ=255)

                                                                                           gggTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACGAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCg                                                       >  2:1277774/1‑150 (MQ=255)

                                                                                             gTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAg                                                     >  2:585499/1‑150 (MQ=255)

                                                                                             gTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAg                                                     <  2:1821614/150‑1 (MQ=255)

                                                                                                ttGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCg                                                  >  1:2379933/1‑150 (MQ=255)

                                                                                                  gCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGAt                                                >  2:2270789/1‑150 (MQ=255)

                                                                                                   ccGCTGCCCGTGGTCGGCGGGCTCCCTGGCG‑CCGCGGGGCTGGCCAGCGGGCTGCCCTGCCTGGACCCGGCGCTACCCCCCCCCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTGGATTTTTTCCAGCGCCGGTGCCGAGGCGATc                                               <  2:1018058/150‑1 (MQ=255)

                                                                                                    cGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTg                                                                                                                                               <  2:1016607/53‑1 (MQ=255)

                                                                                                    cGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTg                                                                                                                                               >  1:1016607/1‑53 (MQ=255)

                                                                                                                tCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCa                                  <  1:585499/150‑1 (MQ=255)

                                                                                                                  gACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGt                                >  2:186323/1‑150 (MQ=255)

                                                                                                                    cGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAATGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCAcgc                                        >  1:362542/1‑140 (MQ=255)

                                                                                                                    cGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAATGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCAcgc                                        <  2:362542/140‑1 (MQ=255)

                                                                                                                     gTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCa                             <  2:2313370/150‑1 (MQ=255)

                                                                                                                        cTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCa                          >  2:1925996/1‑150 (MQ=255)

                                                                                                                           aCTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGAttttt                                                                       >  2:1707088/1‑102 (MQ=255)

                                                                                                                           aCTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGAttttt                                                                       <  1:1707088/102‑1 (MQ=255)

                                                                                                                           aCTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCg                       >  1:1578232/1‑150 (MQ=255)

                                                                                                                           aCTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGGTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCg                       >  2:607337/1‑150 (MQ=255)

                                                                                                                            cTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACAATACACCAGCGAGGCAAGGTTACGGCCAAGGTCATCCGCGTTGATTTTTTCTAGCTCCGGTTCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGt                      <  2:1479427/150‑1 (MQ=255)

                                                                                                                               gcg‑cCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGGGCGTTACGCGACGCAGTTCAGCATCGTCaa                   <  1:2244913/150‑1 (MQ=255)

                                                                                                                               gcg‑cCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTCaa                   >  2:1300961/1‑150 (MQ=255)

                                                                                                                                cg‑ccgcTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTCAAg                  <  2:1578232/150‑1 (MQ=255)

                                                                                                                                cg‑ccgcTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTCAAg                  <  1:1324444/150‑1 (MQ=255)

                                                                                                                                     gctgggctggCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTCAAGCaga              <  1:1088096/150‑1 (MQ=255)

                                                                                                                                       tgggctggCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGc                                                                                 >  2:348229/1‑81 (MQ=255)

                                                                                                                                       tgggctggCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGc                                                                                 <  1:348229/81‑1 (MQ=255)

                                                                                                                                         ggctggCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTc                     <  1:1945199/139‑1 (MQ=255)

                                                                                                                                         ggctggCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTc                     >  2:1945199/1‑139 (MQ=255)

                                                                                                                                             cgCCATCGGGCGGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTCAAGCAGAGCCTgcag      <  2:1110425/149‑1 (MQ=255)

                                                                                                                                               ccAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGAtttt                                                                        >  2:1898413/1‑82 (MQ=255)

                                                                                                                                               ccAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGAtttt                                                                        <  1:1898413/82‑1 (MQ=255)

                                                                                                                                                cAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGc                                                   <  2:2283643/102‑1 (MQ=255)

                                                                                                                                                cAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGc                                                   >  1:2283643/1‑102 (MQ=255)

                                                                                                                                                cAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTCAAGCAGAGCCTGcagcag   >  1:242740/1‑150 (MQ=255)

                                                                                                                                                cAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTCAAGCAGAGCCTGcagcag   >  1:926036/1‑150 (MQ=255)

                                                                                                                                                 aGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTc                                                                     >  1:1209434/1‑83 (MQ=255)

                                                                                                                                                 aGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTc                                                                     <  2:1209434/83‑1 (MQ=255)

                                                                                                                                                 aGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTCAAGCAGAGCCTGCAGCAgg  <  1:875363/150‑1 (MQ=255)

                                                                                                                                                 aGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTCAAGCAGAGCCTGCAGCAgg  >  2:2247733/1‑150 (MQ=255)

TGCCGTACTTCGAACTCACGGCCCAGCACCGGGTCGATGCGGCCTTCGCGGGCGCTTTGGGTAAGGTTGACCGTGTACTGGTCCAGCGCCGGGGTCTTGCCGCTGGCCTTGCTCGACGTGCTCACTGGCG‑CCGCTGGGCTGGCCAGCGGGCTGGCCTGCCTGGACTCGGCGCTACCCTCCACCAGCGCGGCAAGGTTCAGGCGCAGGTCATCGGCGTTGATTTTTTCCAGCTCCGGTGCCGAGGCGATCACCACGCGACGCAGTTCAGCATCGTCAAGCAGAGCCTGCAGCAGG  >  NC_002947/3490763‑3491056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`