Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,491,279 A→T 6.6% L52Q (CTG→CAG)  clpV ← protein ClpV1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,491,2790AT 6.6% 293.9 / 9.3 91L52Q (CTG→CAG) clpVprotein ClpV1
Reads supporting (aligned to +/- strand):  ref base A (42/43);  new base T (2/4);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

GCGGCGACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACG  >  NC_002947/3491133‑3491427
                                                                                                                                                  |                                                                                                                                                    
gcggcgACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTgcagcagca                                                                                                                                                   <  2:2211813/150‑1 (MQ=255)
 cggcgACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGcagcagcag                                                                                                                                                  <  2:2269463/150‑1 (MQ=255)
 cggcgACAGCGCCGGGGTGCGGGTATTACCTTCCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGcagcagcag                                                                                                                                                  <  2:1099296/150‑1 (MQ=255)
  ggcgACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTTGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAgg                                                                                                                                                 <  1:666791/150‑1 (MQ=255)
   gcgACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTgcagca                                                                                                                                                      <  1:1070534/144‑1 (MQ=255)
   gcgACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTgcagca                                                                                                                                                      >  2:1070534/1‑144 (MQ=255)
    cgACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTg                                                                                                                                               <  2:1215464/150‑1 (MQ=255)
     gACAGCGCCGGGGTGCGGGTATTACCTTTCTTGGAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGc                                                                                                                                              <  2:1722422/150‑1 (MQ=255)
     gACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGc                                                                                                                                              <  2:1211099/150‑1 (MQ=255)
       cAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                            <  2:1050915/150‑1 (MQ=255)
       cAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTc                                                                                                                                            >  2:941242/1‑150 (MQ=255)
                ggTGCGGGTATTAGCTTTGTTGCAGGTGCCCCGGGCAGTAACCGTGGGCTCGTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCAc                                                                                                                                   <  2:264978/150‑1 (MQ=255)
                ggTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGAAGCAGGTGCTCCACTTCCAc                                                                                                                                   <  1:1699086/150‑1 (MQ=255)
                       gTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGTTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTaa                                                                                                                            <  1:774769/150‑1 (MQ=255)
                       gTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCAAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTaa                                                                                                                            >  2:2164768/1‑150 (MQ=255)
                         aTTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCAAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATg                                                                                                                          <  1:2164768/150‑1 (MQ=255)
                          ttACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATgg                                                                                                                         <  2:1455841/150‑1 (MQ=255)
                              cTTTCTTGAATGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCt                                                                                                                     >  2:124240/1‑150 (MQ=255)
                               tttCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGc                                                                                                                      >  2:1199452/1‑148 (MQ=255)
                               tttCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGc                                                                                                                      <  1:1199452/148‑1 (MQ=255)
                               tttCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTc                                                                                                                    >  1:1029523/1‑150 (MQ=255)
                               tttCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTc                                                                                                                    <  2:1370114/150‑1 (MQ=255)
                                   ttGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCg                                                                                                                               <  2:1999306/135‑1 (MQ=255)
                                   ttGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCg                                                                                                                               >  1:1999306/1‑135 (MQ=255)
                                   ttGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGAc                                                                                                                >  2:1270424/1‑150 (MQ=255)
                                   ttGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGAc                                                                                                                <  1:1302755/150‑1 (MQ=255)
                                     gAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAg                                                                                                              >  2:1432807/1‑150 (MQ=255)
                                         gTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGGAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGcac                                                                                                          >  1:1576261/1‑150 (MQ=255)
                                           gCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAg                                                                                                        <  1:941242/150‑1 (MQ=255)
                                            cccAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAgg                                                                                                       <  1:2161243/150‑1 (MQ=255)
                                              cAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGgc                                                                                                     >  1:1048771/1‑150 (MQ=255)
                                                gggCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGgccg                                                                                                   >  2:1992715/1‑150 (MQ=255)
                                                    ggTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTcc                                                                                                                   >  2:1958726/1‑130 (MQ=255)
                                                    ggTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTcc                                                                                                                   <  1:1958726/130‑1 (MQ=255)
                                                      tGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCa                                                                                                                                    <  1:1575308/111‑1 (MQ=255)
                                                      tGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCa                                                                                                                                    >  2:1575308/1‑111 (MQ=255)
                                                      tGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCgcgc                                                                                             >  1:368928/1‑150 (MQ=255)
                                                       gACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAgg                                                                                                                                                 >  2:313110/1‑97 (MQ=255)
                                                       gACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAgg                                                                                                                                                 <  1:313110/97‑1 (MQ=255)
                                                       gACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGgccg                                                                                                   >  2:1984573/1‑143 (MQ=255)
                                                       gACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGgccg                                                                                                   <  1:1984573/143‑1 (MQ=255)
                                                        aCCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCtt                                                                                           >  1:1545445/1‑150 (MQ=255)
                                                          cAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTc                                                                                         >  2:1096028/1‑150 (MQ=255)
                                                             cTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                            <  2:1351041/96‑1 (MQ=255)
                                                             cTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                            >  1:1351041/1‑96 (MQ=255)
                                                             cTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCCCAGGGCCGCCGCGCTTTCGGt                                                                                      <  2:242740/150‑1 (MQ=255)
                                                              tGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTg                                                                                     <  1:1992715/150‑1 (MQ=255)
                                                                 gcTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCTCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGcc                                                                                  >  1:519086/1‑150 (MQ=255)
                                                                      cAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCCCGGGGCCGCCGCGCTTTCGGTGGCCGTGCg                                                                             <  1:1270424/150‑1 (MQ=255)
                                                                       aGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCgg                                                                            >  2:1203452/1‑150 (MQ=255)
                                                                         gCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCt                                                                          <  1:661036/150‑1 (MQ=255)
                                                                          cGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTg                                                                         <  1:688422/150‑1 (MQ=255)
                                                                           gTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTgg                                                                        >  1:1058858/1‑150 (MQ=255)
                                                                                  gacgacCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTTCGCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGtt                                                                 >  1:1373351/1‑150 (MQ=255)
                                                                                  gacgacCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGtt                                                                 <  1:1096028/150‑1 (MQ=255)
                                                                                   acgacCTGGTAGTGGCGCAGGATTGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTg                                                                >  1:604611/1‑150 (MQ=255)
                                                                                     gacCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAg                                                              <  2:409279/150‑1 (MQ=255)
                                                                                         tGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGTAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGGCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTg                                                          <  1:2293970/150‑1 (MQ=255)
                                                                                          ggTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTgg                                                         <  2:493464/150‑1 (MQ=255)
                                                                                                ggCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACa                                                   <  1:97771/150‑1 (MQ=255)
                                                                                                    cAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGa                                                               <  1:1715301/134‑1 (MQ=255)
                                                                                                    cAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGa                                                               >  2:1715301/1‑134 (MQ=255)
                                                                                                    cAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAgaga                                               >  2:43124/1‑150 (MQ=255)
                                                                                                         tCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCa                                          >  2:726766/1‑150 (MQ=255)
                                                                                                           ggCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTAGTTGAGCTTGGCGAACAGAGACTTCAgg                                        <  2:368928/150‑1 (MQ=255)
                                                                                                              gcCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAg                                         >  2:1947617/1‑146 (MQ=255)
                                                                                                              gcCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAg                                         <  1:1947617/146‑1 (MQ=255)
                                                                                                              gcCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTc                                     >  2:13959/1‑150 (MQ=255)
                                                                                                              gcCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTc                                     >  1:2074760/1‑150 (MQ=255)
                                                                                                              gcCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTc                                     >  1:588810/1‑150 (MQ=255)
                                                                                                               ccAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTca                                    <  1:929029/150‑1 (MQ=255)
                                                                                                                 aGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCAGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTcaca                                  >  1:1716177/1‑150 (MQ=255)
                                                                                                                  ggTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACaa                                 <  2:2074760/150‑1 (MQ=255)
                                                                                                                   gTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAg                                >  2:482590/1‑150 (MQ=255)
                                                                                                                   gTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGAGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAg                                <  2:1892742/150‑1 (MQ=255)
                                                                                                                      gCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGc                                                                              >  2:105775/1‑101 (MQ=255)
                                                                                                                      gCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGc                                                                              <  1:105775/101‑1 (MQ=255)
                                                                                                                      gCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCg                             >  2:2806/1‑150 (MQ=255)
                                                                                                                             ttgttgTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTc                                                                                         <  2:1080478/83‑1 (MQ=255)
                                                                                                                             ttgttgTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTc                                                                                         >  1:1080478/1‑83 (MQ=255)
                                                                                                                             ttgttgTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTc                                                                    <  2:546321/104‑1 (MQ=255)
                                                                                                                             ttgttgTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTc                                                                    >  1:546321/1‑104 (MQ=255)
                                                                                                                                  gtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTaa                 <  2:1794414/150‑1 (MQ=255)
                                                                                                                                  gtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTaa                 <  1:13959/150‑1 (MQ=255)
                                                                                                                                  gtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTaa                 <  1:1432807/150‑1 (MQ=255)
                                                                                                                                   tggAGCACCTGCTGCTGCAGTTGCTCGACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCt                                                                                            <  2:870258/72‑1 (MQ=21)
                                                                                                                                   tggAGCACCTGCTGCTGCAGTTGCTCGACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCt                                                                                            >  1:870258/3‑74 (MQ=21)
                                                                                                                                    cGAGCAACTGTAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAgg               >  2:336546/1‑150 (MQ=255)
                                                                                                                                     gAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGtt                                                                 >  1:661822/1‑99 (MQ=255)
                                                                                                                                     gAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGtt                                                                 <  2:661822/99‑1 (MQ=255)
                                                                                                                                       gCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAt            >  1:1687348/1‑150 (MQ=255)
                                                                                                                                        cacctgctgctgCAGTTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGc                              >  2:682164/4‑131 (MQ=255)
                                                                                                                                         aaCTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACa                                                   <  2:1966439/109‑1 (MQ=255)
                                                                                                                                         aaCTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACa                                                   >  1:1966439/1‑109 (MQ=255)
                                                                                                                                         aaCTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtgt          >  2:1361689/1‑150 (MQ=255)
                                                                                                                                         aaCTGCAGCAGCAGGTGCTCCACTTCCACCGCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCTGCTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtgt          <  1:43124/150‑1 (MQ=255)
                                                                                                                                          aCTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtgtg         >  2:1523390/1‑150 (MQ=255)
                                                                                                                                             gcagcagcaGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGaa      <  2:1545445/150‑1 (MQ=255)
                                                                                                                                             gcagcagcaGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGaa      <  1:1047655/150‑1 (MQ=255)
                                                                                                                                                 cagcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACg  >  1:1844184/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCGGCGACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACG  >  NC_002947/3491133‑3491427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑