Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,542,364 C→T 8.5% P134P (CCG→CCA)  galE ← UDP‑glucose 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,542,3640CT 8.5% 327.2 / 15.2 117P134P (CCG→CCA) galEUDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (50/57);  new base T (6/4);  total (56/61)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01

Only 100 of 121 total aligned reads displayed.

AGATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTCTG  >  NC_002947/3542215‑3542513
                                                                                                                                                     |                                                                                                                                                     
aGATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATAc                                                                                                                                                       >  1:915611/1‑150 (MQ=255)
 gATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACg                                                                                                                                                      >  2:1107765/1‑150 (MQ=255)
 gATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACg                                                                                                                                                      >  1:233987/1‑150 (MQ=255)
    gggTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGt                                                                                                                                                   <  2:469794/150‑1 (MQ=255)
       tttCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGa                                                                                                                                                >  1:338040/1‑150 (MQ=255)
       tttCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGa                                                                                                                                                >  2:588992/1‑150 (MQ=255)
         tCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGagc                                                                                                                                              <  2:1782598/150‑3 (MQ=255)
            ggCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATATGGGTTGATCGGc                                                                                                                                           >  1:1171090/1‑150 (MQ=255)
             gCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGcc                                                                                                                                          <  1:2145022/150‑1 (MQ=255)
                 gtcaCGCTCGACCAGCTGGCCTACCGGTTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCACAGGCCAGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCAGTTGCTCAACCCACACCGGTTGATCGGCCCCtt                                                                                                                                      <  2:235562/147‑1 (MQ=255)
                  tGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTg                                                                                                                                     <  1:455949/150‑1 (MQ=255)
                   gACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTgg                                                                                                                                    >  2:1091807/1‑150 (MQ=255)
                    gcGCTCCCATAGCTGGCCTTCCGTGTCAGCGCACGCTGCATTGAAATAGCGCAGTCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGCAGCCGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgt                                                                                                                                   <  2:338040/149‑1 (MQ=255)
                    aCGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgt                                                                                                                                   <  1:1775661/150‑1 (MQ=255)
                      gCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgt                                                                                                                                 <  2:2121380/150‑1 (MQ=255)
                      gCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgt                                                                                                                                 >  1:2312076/1‑150 (MQ=255)
                        tCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATATGGGTTGATCGGCCCCTTGgtgtgt                                                                                                                               <  2:1171090/150‑1 (MQ=255)
                        tCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGTGTTGATCGGCCCCTTGgtgtgt                                                                                                                               <  2:115944/150‑1 (MQ=255)
                        tCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgtgt                                                                                                                               <  2:1841771/150‑1 (MQ=255)
                          gCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGc                                                                                                                             <  2:2313947/150‑1 (MQ=255)
                                   gCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTCACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgat                                                                                                                    >  2:1100065/1‑150 (MQ=255)
                                    ccTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatc                                                                                                                   <  2:967405/150‑1 (MQ=255)
                                     cTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatcg                                                                                                                  <  1:1524867/150‑1 (MQ=255)
                                           ggTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGt                                                                                                            >  1:1286750/1‑150 (MQ=255)
                                                gcgcCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGc                                                                                                       <  1:733700/150‑1 (MQ=255)
                                                gcgcCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGc                                                                                                       >  1:1837696/1‑150 (MQ=255)
                                                 cgcCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGc                                                                                                       >  2:1227885/1‑149 (MQ=255)
                                                 cgcCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGc                                                                                                       <  1:1227885/149‑1 (MQ=255)
                                                   cccGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCggg                                                                                                    <  1:1101016/150‑1 (MQ=255)
                                                    ccGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGt                                                                                                   >  1:817384/1‑150 (MQ=255)
                                                        tGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCACTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGcc                                                                                               <  1:1974335/150‑1 (MQ=255)
                                                            ttGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCat                                                                                                                        <  1:1112876/121‑1 (MQ=255)
                                                               aaaTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGATGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATAc                                                                                                                                                       >  1:975109/1‑87 (MQ=255)
                                                                 aTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggc                                                                                      <  2:233987/150‑1 (MQ=255)
                                                                          gCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTTGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCga                                                                             <  2:915611/150‑1 (MQ=255)
                                                                           cTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaa                                                                            <  1:1007361/150‑1 (MQ=255)
                                                                               ccGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGaaa                                                                        >  1:132859/1‑150 (MQ=255)
                                                                                cGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGaaaa                                                                       <  1:588992/150‑1 (MQ=255)
                                                                                cGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGaaaa                                                                       <  1:2122953/150‑1 (MQ=255)
                                                                                 gACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTGTACGGCGGCACTCGAAGAAAac                                                                      >  1:158849/1‑150 (MQ=255)
                                                                                    ttCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgt                                                                                                                                 <  1:1604784/88‑1 (MQ=255)
                                                                                    ttCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgt                                                                                                                                 >  2:1604784/1‑88 (MQ=255)
                                                                                       aaaCCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCgg                                                                                                     <  2:1278495/113‑1 (MQ=255)
                                                                                       aaaCCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCgg                                                                                                     >  1:1278495/1‑113 (MQ=255)
                                                                                       aaaCCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaa                                                                            <  2:1638537/138‑1 (MQ=255)
                                                                                       aaaCCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaa                                                                            >  1:1638537/1‑138 (MQ=255)
                                                                                        aaCCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAg                                                                 <  1:2210683/148‑1 (MQ=255)
                                                                                        aaCCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAg                                                                 >  2:2210683/1‑148 (MQ=255)
                                                                                          ccATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGtata                                                                                          >  1:886101/1‑121 (MQ=255)
                                                                                          ccATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGc                                                             <  2:672373/150‑1 (MQ=255)
                                                                                          ccATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGc                                                             >  2:2345244/1‑150 (MQ=255)
                                                                                          ccATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGc                                                             <  2:1882872/150‑1 (MQ=255)
                                                                                               ggCCTGATCGAAGTTCTCAAGAATCTGCTCGACCAGTCATTTGCTCAACCCATACGGGTTGTGCGGCCCCTGGGTGTGTGCTTCTTCGTCTTAAACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGAt                                                        <  2:215172/150‑1 (MQ=255)
                                                                                                   cGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGTCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTTCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCg                                                    <  2:1018947/150‑1 (MQ=255)
                                                                                                   cGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCg                                                    >  2:340454/1‑150 (MQ=255)
                                                                                                        gAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCAtt                                               <  2:817384/150‑1 (MQ=255)
                                                                                                         aaGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcga                                                                                                                     <  2:138148/79‑1 (MQ=255)
                                                                                                         aaGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcga                                                                                                                     >  1:138148/1‑79 (MQ=255)
                                                                                                          aGTCCTCAAGAATCTGCTCGACACTCCATTTGATCAACCCATACTGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGa                                             <  1:538594/150‑1 (MQ=255)
                                                                                                            tCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGAcc                                           >  2:858398/1‑150 (MQ=255)
                                                                                                              cTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaa                                                                            <  2:1404543/115‑1 (MQ=255)
                                                                                                              cTCAAGAATCTGCTCGACCATCCATTTGCACAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAACCACCAGGTGCCTGATGCCGGCAATGACGAt                                         >  1:1028156/1‑150 (MQ=255)
                                                                                                               tCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATg                                        >  1:1340993/1‑150 (MQ=255)
                                                                                                               tCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATg                                        >  1:314624/1‑150 (MQ=255)
                                                                                                                caacaaTCTTCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATgg                                       <  2:1465434/150‑1 (MQ=255)
                                                                                                                cAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATgg                                       >  1:1997132/1‑150 (MQ=255)
                                                                                                                cAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCACTTGGTTTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATgg                                       <  2:1530876/150‑1 (MQ=255)
                                                                                                                 aaGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCg                                                    <  2:490463/136‑1 (MQ=255)
                                                                                                                   gAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCt                                    >  1:1157986/1‑150 (MQ=255)
                                                                                                                      tCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCt                                    >  2:1524612/1‑147 (MQ=255)
                                                                                                                      tCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCt                                    <  1:1524612/147‑1 (MQ=255)
                                                                                                                      tCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTgca                                 >  2:1193212/1‑150 (MQ=255)
                                                                                                                        tGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCa                                                 >  1:2134650/1‑132 (MQ=255)
                                                                                                                         gCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGtata                                                                                          <  2:964154/90‑1 (MQ=255)
                                                                                                                         gCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGtata                                                                                          >  1:964154/1‑90 (MQ=255)
                                                                                                                          cTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGcagcag                             >  1:2263608/1‑150 (MQ=255)
                                                                                                                           tCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAgg                            <  1:340454/150‑1 (MQ=255)
                                                                                                                           tCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAgg                            >  1:1515602/1‑150 (MQ=255)
                                                                                                                             gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGt                                                                                             >  1:1239914/1‑83 (MQ=255)
                                                                                                                                  tccatTTGCTCAACCCGTACGGGTTGTTCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAggg                     <  2:1028156/150‑1 (MQ=255)
                                                                                                                                  tccatTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGGTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAggg                     >  2:253649/1‑150 (MQ=255)
                                                                                                                                   ccatTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGt                    <  2:1273275/150‑1 (MQ=255)
                                                                                                                                    catTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg                   <  2:1837696/150‑1 (MQ=255)
                                                                                                                                    catTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg                   <  1:1091807/150‑1 (MQ=255)
                                                                                                                                     atTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTgg                  <  2:2263608/150‑1 (MQ=255)
                                                                                                                                      gccgatCAACCCGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGc                 >  2:1562290/6‑150 (MQ=255)
                                                                                                                                        cgatCAACCCGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTg               >  1:626382/4‑150 (MQ=255)
                                                                                                                                         gatCAACCCGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTg                                                              <  1:589331/100‑1 (MQ=255)
                                                                                                                                         gCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGc              <  1:2345244/150‑1 (MQ=255)
                                                                                                                                          cTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGc                                                                                                       >  1:1469834/1‑60 (MQ=255)
                                                                                                                                          cTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCg             >  1:745185/1‑150 (MQ=255)
                                                                                                                                          atCAACCCGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAgg                            <  1:828566/134‑1 (MQ=255)
                                                                                                                                          atCAACCCGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAgg                            >  2:828566/2‑135 (MQ=255)
                                                                                                                                           tCAACCCGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGGTGCGa            >  2:865138/1‑150 (MQ=255)
                                                                                                                                           tCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAgg                      >  2:2192901/1‑140 (MQ=255)
                                                                                                                                               cccATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACgtt        <  1:858398/150‑1 (MQ=255)
                                                                                                                                                  aTACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGc                                                             >  1:923380/1‑94 (MQ=255)
                                                                                                                                                  aTACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGc                                                             <  2:923380/94‑1 (MQ=255)
                                                                                                                                                  aTACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCT‑CAGCAGGGAAAGGGTGGCTGCGACGTTGTTc    >  1:109146/1‑150 (MQ=255)
                                                                                                                                                     cGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATCAGGCGGCATGTGTTGAAAACACCAGGGGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTCTg  <  1:292113/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTCTG  >  NC_002947/3542215‑3542513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑