Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,590,368 G→C 5.1% R282R (CGG→CGC)  nicP‑I → porin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,590,3680GC 5.1% 319.3 / 6.5 98R282R (CGG→CGC) nicP‑Iporin‑like protein
Reads supporting (aligned to +/- strand):  ref base G (50/43);  new base C (3/2);  total (53/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

CAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACGTTA  >  NC_002947/3590219‑3590517
                                                                                                                                                     |                                                                                                                                                     
caacaaGGCTTTGTTGGCCTGAAGAACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCGAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCgg                                                                                                                                                       >  2:27721/1‑150 (MQ=255)
 aacaaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGc                                                                                                                                                      >  1:50861/1‑150 (MQ=255)
 aacaaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGc                                                                                                                                                      >  1:29969/1‑150 (MQ=255)
    aaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATg                                                                                                                                                   >  1:968725/1‑150 (MQ=255)
    aaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATg                                                                                                                                                   >  1:26638/1‑150 (MQ=255)
     aGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATgg                                                                                                                                                  >  2:616793/1‑150 (MQ=255)
       gCTTTGTTCGCCTGTACCCCCGCCAGCCTGTCTGGGAGTGTGGACTCAGTGCCAACCTGGGTTCAGTCATGGTGCGGGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCg                                                                                                                                                <  1:44587/150‑1 (MQ=255)
           tgttgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGca                                                                                                                                            <  1:77375/150‑1 (MQ=255)
            gttgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGcac                                                                                                                                           >  1:2124927/1‑150 (MQ=255)
            gttgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGcac                                                                                                                                           >  2:2228162/1‑150 (MQ=255)
             ttgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGcaca                                                                                                                                          >  1:1826592/1‑150 (MQ=255)
             ttgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGcaca                                                                                                                                          >  2:1193486/1‑150 (MQ=255)
              tgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAg                                                                                                                                         <  2:932074/150‑1 (MQ=255)
                gCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCt                                                                                                                                       >  2:2026844/1‑150 (MQ=255)
                   ttaaGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTcc                                                                                                                                    >  2:1009267/3‑150 (MQ=255)
                   tGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTcc                                                                                                                                    <  1:2111099/150‑1 (MQ=255)
                        cacaGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGca                                                                                                                                            <  1:1678684/137‑1 (MQ=255)
                        cacaGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGca                                                                                                                                            >  2:1678684/1‑137 (MQ=255)
                           aGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGc                                                                                                                            >  2:2262884/1‑150 (MQ=255)
                           aGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAACTTCCATACCGGc                                                                                                                            >  1:888221/1‑150 (MQ=255)
                                  ccGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAgg                                                                                                                     >  2:1415290/1‑150 (MQ=255)
                                  ccGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTATCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAgg                                                                                                                     <  1:715207/150‑1 (MQ=255)
                                   cGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCNGNTNACTGCCCGGCATGGCNGGCACAGCTTCCATGCCGGCTACCAggg                                                                                                                    <  1:1415290/150‑1 (MQ=255)
                                       ggCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGTAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCggg                                                                                                                                              <  1:643653/120‑1 (MQ=255)
                                       ggCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGTAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCggg                                                                                                                                              >  2:643653/1‑120 (MQ=255)
                                       ggCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCAtt                                                                                                                <  1:2228162/150‑1 (MQ=255)
                                           aCTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTc                                                                                                                                     >  2:123812/1‑125 (MQ=255)
                                           aCTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTc                                                                                                                                     <  1:123812/125‑1 (MQ=255)
                                            cTGGGTCCTCAGTGCCAACCTGGATTATTTCACCGCGCGGGAGGAAGGCGATGCGCTGATGGGCAACCTCGTCAACCACGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATgg                                                                                                           <  2:507712/150‑1 (MQ=255)
                                            cTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATgg                                                                                                           <  1:513859/150‑1 (MQ=255)
                                             tGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACCGCTTCCATGCCGGCTACCACGGCATTTATGGc                                                                                                          <  2:26638/150‑1 (MQ=255)
                                              gggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGc                                                                                                                   >  2:1210638/1‑140 (MQ=255)
                                              gggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGc                                                                                                                   <  1:1210638/140‑1 (MQ=255)
                                               ggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGa                                                                                                        <  2:2047247/150‑1 (MQ=255)
                                               ggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGCCTACCAGGGCATTTATGGCGa                                                                                                        >  2:1578840/1‑150 (MQ=255)
                                                    tCAGTGCCAACCTTGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGcc                                                                                                   >  1:1876669/1‑150 (MQ=255)
                                                    tCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGcc                                                                                                   >  2:1207818/1‑150 (MQ=255)
                                                      aGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGc                                                                                                                                                 <  2:1393304/102‑1 (MQ=255)
                                                      aGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGc                                                                                                                                                 >  1:1393304/1‑102 (MQ=255)
                                                      aGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCg                                                                                                 >  1:1915708/1‑150 (MQ=255)
                                                           cAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTccc                                                                                            >  1:1136278/1‑150 (MQ=255)
                                                             aCCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCAc                                                                                          >  1:1563031/1‑150 (MQ=255)
                                                               cTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCCTTTCTGGCGATAGCCCGTTACCACgg                                                                                        >  2:748160/1‑150 (MQ=255)
                                                                tGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACggg                                                                                       >  1:2401659/1‑150 (MQ=255)
                                                                   gTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCCCATATGGCGATAGCCCGTTCCCACGGGTAt                                                                                    <  1:63623/150‑1 (MQ=255)
                                                                            ccGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACa                                                                           <  1:616793/150‑1 (MQ=255)
                                                                            ccGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACa                                                                           >  2:662678/1‑150 (MQ=255)
                                                                             cgcgcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACAt                                                                          >  2:711383/1‑150 (MQ=255)
                                                                                gcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACAtctc                                                                       <  2:968774/150‑1 (MQ=255)
                                                                                  gTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGc                                                                     >  2:1628265/1‑150 (MQ=255)
                                                                                    gAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCg                                                                   <  2:1826592/150‑1 (MQ=255)
                                                                                      gCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGtt                                                                 <  1:357715/150‑1 (MQ=255)
                                                                                      gCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGtt                                                                 >  2:2286017/1‑150 (MQ=255)
                                                                                       cAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTg                                                                <  1:711383/150‑1 (MQ=255)
                                                                                       cAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTg                                                                <  2:2124927/150‑1 (MQ=255)
                                                                                       cAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGGTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTg                                                                >  2:1822803/1‑150 (MQ=255)
                                                                                        aaGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTgg                                                               >  2:1760737/1‑150 (MQ=255)
                                                                                         aGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTcc                                                                                             >  1:1525064/1‑119 (MQ=255)
                                                                                         aGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTcc                                                                                             <  2:1525064/119‑1 (MQ=255)
                                                                                         aGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTggg                                                              >  1:1686788/1‑150 (MQ=255)
                                                                                         aGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTggg                                                              >  1:1726845/1‑150 (MQ=255)
                                                                                                gcgcTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAg                                                       <  1:1193486/150‑1 (MQ=255)
                                                                                                gcgcTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGCGCAAAGat                                                       <  2:1802117/150‑2 (MQ=255)
                                                                                                       tgGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGa                                                >  2:565942/1‑150 (MQ=255)
                                                                                                       tgGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGa                                                <  1:27721/150‑1 (MQ=255)
                                                                                                       tgGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGa                                                <  1:748160/150‑1 (MQ=255)
                                                                                                          gCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGGATTTATGGCGa                                                                                                        <  2:1427877/91‑1 (MQ=255)
                                                                                                          gCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGGATTTATGGCGa                                                                                                        >  1:1427877/1‑91 (MQ=255)
                                                                                                            aaCATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTAt                                           >  1:995897/1‑150 (MQ=255)
                                                                                                            aaCATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTAt                                           <  1:2286017/150‑1 (MQ=255)
                                                                                                                  gACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGttt                                     <  1:565942/150‑1 (MQ=255)
                                                                                                                     aaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGcc                                  <  2:1136278/150‑1 (MQ=255)
                                                                                                                        cAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTac                               <  2:2041276/150‑1 (MQ=255)
                                                                                                                        cAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTACCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTac                               >  1:1303195/1‑150 (MQ=255)
                                                                                                                           gCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGcaca                                                                                                                                          >  1:2097740/1‑40 (MQ=255)
                                                                                                                           gCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGcaca                                                                                                                                          <  2:2097740/40‑1 (MQ=255)
                                                                                                                           gCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAAc                                                                            >  2:409234/1‑102 (MQ=255)
                                                                                                                           gCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAAc                                                                            <  1:409234/102‑1 (MQ=255)
                                                                                                                             gTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGttt                                     <  1:537978/139‑1 (MQ=255)
                                                                                                                             gTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGttt                                     >  2:537978/1‑139 (MQ=255)
                                                                                                                                 ttctCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGaa                      >  1:1671394/1‑150 (MQ=255)
                                                                                                                                 ttctCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGaa                      <  1:662678/150‑1 (MQ=255)
                                                                                                                                  tctcGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAAc                     <  2:50861/150‑1 (MQ=255)
                                                                                                                                    tcGCTGTTCACTGCCCGGCATGGCGGGAACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGc                   <  2:968725/150‑1 (MQ=255)
                                                                                                                                      gCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTCTGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGt                                                     >  2:1566360/1‑114 (MQ=255)
                                                                                                                                      gCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGt                                                     <  1:1566360/114‑1 (MQ=255)
                                                                                                                                      gCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTc                 <  1:2026844/150‑1 (MQ=255)
                                                                                                                                      gCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTc                 >  2:1311976/1‑150 (MQ=255)
                                                                                                                                            ctgtGCCCGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCa           >  1:2263058/4‑150 (MQ=255)
                                                                                                                                             aCTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTgg                                                               >  2:1949916/1‑97 (MQ=255)
                                                                                                                                             aCTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTgg                                                               <  1:1949916/97‑1 (MQ=255)
                                                                                                                                             aCTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAg          <  2:1686788/150‑1 (MQ=255)
                                                                                                                                             aCTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAg          <  2:995897/150‑1 (MQ=255)
                                                                                                                                               tGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGcc                                                                    >  1:2221741/1‑90 (MQ=255)
                                                                                                                                               tGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGcc                                                                    <  2:2221741/90‑1 (MQ=255)
                                                                                                                                               tGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGt        >  2:874504/1‑150 (MQ=255)
                                                                                                                                               tGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGt        >  2:2022415/1‑150 (MQ=255)
                                                                                                                                                  ccGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACg     >  1:147464/1‑150 (MQ=255)
                                                                                                                                                  ccGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGa                                         >  2:1426910/1‑114 (MQ=255)
                                                                                                                                                     gCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACGTTa  >  2:1714265/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACGTTA  >  NC_002947/3590219‑3590517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑