Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,638,706 T→G 7.2% L220R (CTG→CGG)  PP_3205 → fumarylacetoacetate hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,638,7060TG 7.2% 293.9 / 11.4 99L220R (CTG→CGG) PP_3205fumarylacetoacetate hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (51/40);  new base G (5/2);  total (56/43)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

TGTCAGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGA  >  NC_002947/3638560‑3638853
                                                                                                                                                  |                                                                                                                                                   
tGTCAGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCa                                                                                                                                                  >  2:247398/1‑150 (MQ=255)
 gTCAGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTTGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCAGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCAc                                                                                                                                                 <  2:716304/150‑1 (MQ=255)
    aGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTg                                                                                                                                              >  2:2312656/1‑150 (MQ=255)
      ccTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCa                                                                                                                                            <  1:2312656/150‑1 (MQ=255)
       cTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCAc                                                                                                                                           <  1:1566661/150‑1 (MQ=255)
        tGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACt                                                                                                                                          <  2:1072715/150‑1 (MQ=255)
         gCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTg                                                                                                                                         <  1:1512234/150‑1 (MQ=255)
            ggCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGaa                                                                                                                                      >  2:464579/1‑150 (MQ=255)
             gCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAAc                                                                                                                                     >  2:2389041/1‑150 (MQ=255)
             gCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAAc                                                                                                                                     <  1:393569/150‑1 (MQ=255)
              cccTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACg                                                                                                                                    >  1:1563710/1‑150 (MQ=255)
              cccTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACg                                                                                                                                    <  1:279938/150‑1 (MQ=255)
                  ggCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGa                                                                                                                                >  1:2183124/1‑150 (MQ=255)
                  ggCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGa                                                                                                                                <  1:906466/150‑1 (MQ=255)
                   gCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAg                                                                                                                               >  2:2074931/1‑150 (MQ=255)
                   gCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAg                                                                                                                               <  2:2183124/150‑1 (MQ=255)
                     aCCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTg                                                                                                                             <  1:247398/150‑1 (MQ=255)
                                tGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCCGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGACGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATc                                                                                                                  >  1:19330/1‑150 (MQ=255)
                                    gACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCa                                                                                                              >  1:1826988/1‑150 (MQ=255)
                                    gACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGCGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCa                                                                                                              >  2:1347283/1‑150 (MQ=255)
                                       cggcttcggcttTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGAGAGTGGTTCGGCCATCCCCATgg                                                                                                           >  1:1284353/1‑150 (MQ=255)
                                               gcttTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCa                                                                                                   >  2:886567/1‑150 (MQ=255)
                                                 tttCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCCTGGCGgcgcaaag                                                                                                 >  1:646970/1‑146 (MQ=255)
                                                 tttCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCACGGAAGACATCGGCGAGGCCTGTTTCGACGTCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATg                                                                                                 >  1:1498832/1‑150 (MQ=255)
                                                    ctcCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGCCGCCATgggg                                                                                              <  1:1915813/150‑4 (MQ=255)
                                                     tcCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACt                                                                                             >  2:238816/1‑150 (MQ=255)
                                                       cAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTACACTGGAACGGCGAg                                                                                                                               <  2:1946344/114‑1 (MQ=255)
                                                       cAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGGCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTACACTGGAACGGCGAg                                                                                                                               >  1:1946344/1‑114 (MQ=255)
                                                        aGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCTCTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCg                                                                                          <  2:866872/150‑1 (MQ=255)
                                                        aGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCg                                                                                          <  1:2328219/150‑1 (MQ=255)
                                                          gCCAAGCCCGCCGGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACCCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGt                                                                                        >  1:2065811/1‑150 (MQ=255)
                                                          gCCAAGCCCGCCAGCAGTTTCGCGCACGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGt                                                                                        <  2:265520/150‑1 (MQ=255)
                                                            cAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGttt                                                                                      >  1:2320046/1‑150 (MQ=255)
                                                            cAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGttt                                                                                      <  1:464579/150‑1 (MQ=255)
                                                            cAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGttt                                                                                      >  2:2298112/1‑150 (MQ=255)
                                                            cAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATAGCGGCGCCATGCACTTCGGttt                                                                                      >  2:986915/1‑150 (MQ=255)
                                                            cAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGCACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGttt                                                                                      >  1:2293840/1‑150 (MQ=255)
                                                               gCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTcca                                                                                   <  1:2074931/150‑1 (MQ=255)
                                                                cccGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTg                                                                                                                                         >  1:992090/1‑95 (MQ=255)
                                                                cccGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTg                                                                                                                                         <  2:992090/95‑1 (MQ=255)
                                                                 ccGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTccacc                                                                                 >  2:1437261/1‑150 (MQ=255)
                                                                 ccGCCAGCAGTTTCGCGCCCGTAGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTccacc                                                                                 >  1:933346/1‑150 (MQ=255)
                                                                  cGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTccacca                                                                                >  1:1575765/1‑150 (MQ=255)
                                                                     cagcagTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGtt                                                                             <  2:1445605/150‑1 (MQ=255)
                                                                      agcagTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTg                                                                            >  2:1251425/1‑150 (MQ=255)
                                                                       gcagTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGa                                                                           <  1:748442/150‑1 (MQ=255)
                                                                           tttCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGc                                                                       >  2:1677517/1‑150 (MQ=255)
                                                                                 gcCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATg                                                                                                 >  2:2097258/1‑118 (MQ=255)
                                                                                 gcCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATg                                                                                                 <  1:2097258/118‑1 (MQ=255)
                                                                                 gcCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATgc                                                                 <  2:19330/150‑1 (MQ=255)
                                                                                  cccGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCCCCAGTTGATTGCCCATgcg                                                                >  2:1089098/1‑150 (MQ=255)
                                                                                      ttGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGgcgc                                                            <  1:2298112/150‑1 (MQ=255)
                                                                                                cccGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgacgcgacg                                                  >  2:1447800/1‑150 (MQ=255)
                                                                                                 ccGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCAt                                                                                                  <  1:2363144/101‑1 (MQ=255)
                                                                                                 ccGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCAt                                                                                                  >  2:2363144/1‑101 (MQ=255)
                                                                                                 ccGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgacgcgacgc                                                 >  2:613450/1‑150 (MQ=255)
                                                                                                 ccGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgacgcgacgc                                                 <  1:1914748/150‑1 (MQ=255)
                                                                                                  cGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgac                                                        <  1:758702/142‑1 (MQ=255)
                                                                                                  cGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgac                                                        >  2:758702/1‑142 (MQ=255)
                                                                                                     aCGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGa                                             <  1:1447800/150‑1 (MQ=255)
                                                                                                         aCTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTg                                              <  1:1597618/145‑1 (MQ=255)
                                                                                                         aCTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTg                                              >  2:1597618/1‑145 (MQ=255)
                                                                                                          cTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCg                                        <  2:1575765/150‑1 (MQ=255)
                                                                                                          cTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCg                                        >  1:2166525/1‑150 (MQ=255)
                                                                                                           tCGGCGAGGCCTGGCGCGCCGGCCGCGTGCACGTGCCGCCGCACGTGCACCGGAACGGCGAGTGGTTCGGCCAGCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCgg                                       <  1:413396/150‑1 (MQ=255)
                                                                                                           tCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCTCTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCgg                                       <  2:1563710/150‑1 (MQ=255)
                                                                                                           tCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCg                                                                                          <  2:497100/99‑1 (MQ=255)
                                                                                                           tCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCg                                                                                          >  1:497100/1‑99 (MQ=255)
                                                                                                           tCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCgg                                       >  2:474973/1‑150 (MQ=255)
                                                                                                           tCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCgg                                       <  1:238816/150‑1 (MQ=255)
                                                                                                           tCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCgg                                       >  2:2186847/1‑150 (MQ=255)
                                                                                                           tCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGAACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCgg                                       >  2:651562/1‑150 (MQ=255)
                                                                                                               ggAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCAc                                   <  2:285026/149‑1 (MQ=255)
                                                                                                                gAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGc                                                                                                >  1:902711/1‑88 (MQ=255)
                                                                                                                gAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGc                                                                                                <  2:902711/88‑1 (MQ=255)
                                                                                                                   gCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCTACGCTTGAGTGCGGGCACGCTa                               >  2:737984/1‑150 (MQ=255)
                                                                                                                     cTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgac                                                        >  1:571571/1‑123 (MQ=255)
                                                                                                                     cTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgac                                                        <  2:571571/123‑1 (MQ=255)
                                                                                                                        gcgcGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGTGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTggg                          >  1:515980/1‑150 (MQ=255)
                                                                                                                           cgACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTc                       <  1:1677517/150‑1 (MQ=255)
                                                                                                                            gACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTc                                                                                                                         <  1:378576/51‑1 (MQ=255)
                                                                                                                            gACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTc                                                                                                                         >  2:378576/1‑51 (MQ=255)
                                                                                                                                 ccGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGGGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTg                            <  1:1949709/139‑1 (MQ=255)
                                                                                                                                 ccGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTg                            >  2:1949709/1‑139 (MQ=255)
                                                                                                                                  cGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATAGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCAcc                <  2:1826988/150‑1 (MQ=255)
                                                                                                                                    agTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCgt              >  1:1178490/2‑150 (MQ=255)
                                                                                                                                     gTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGtt                                                                                       >  2:832741/1‑76 (MQ=37)
                                                                                                                                     gTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGtt                                                                                       <  1:832741/76‑1 (MQ=37)
                                                                                                                                     gTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCgtg             >  1:1431531/1‑150 (MQ=255)
                                                                                                                                     gTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCgtg             >  1:700268/1‑150 (MQ=255)
                                                                                                                                     gTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCACCCCCATGGCgccgccg                                                                                                   <  2:663609/64‑2 (MQ=16)
                                                                                                                                     gTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCACCCCCATGGCgccgccg                                                                                                   >  1:663609/1‑63 (MQ=18)
                                                                                                                                       gCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTc           >  1:1758875/1‑150 (MQ=255)
                                                                                                                                         aCTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCa         >  2:1711070/1‑150 (MQ=255)
                                                                                                                                          cTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCAt                                                                                                             <  2:415322/49‑1 (MQ=255)
                                                                                                                                          cTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCAt                                                                                                             >  1:415322/1‑49 (MQ=255)
                                                                                                                                           ttGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAAc       >  1:710664/1‑150 (MQ=255)
                                                                                                                                             gCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGc     >  2:1564081/1‑150 (MQ=255)
                                                                                                                                                gCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGa  >  1:510905/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGTCAGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGA  >  NC_002947/3638560‑3638853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑