Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,726,459 C→A 6.5% Q357K (CAA→AAA)  PP_3291 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,726,4590CA 6.5% 499.6 / 15.6 156Q357K (CAA→AAA) PP_3291metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base C (68/76);  new base A (5/5);  total (75/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

Only 100 of 156 total aligned reads displayed.

ACGGTCGATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGAATGGTT  >  NC_002947/3726312‑3726604
                                                                                                                                                   |                                                                                                                                                 
   gTCGATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGcc                                                                                                                                              >  2:1375656/1‑150 (MQ=255)
    tCGATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCg                                                                                                                                             >  2:830532/1‑150 (MQ=255)
     cGATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATACCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACTCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCgg                                                                                                                                            <  1:1953653/150‑1 (MQ=255)
      gATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCggg                                                                                                                                           <  2:2335771/150‑1 (MQ=255)
       aTGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCgggg                                                                                                                                          <  1:1202278/150‑1 (MQ=255)
       aTGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCgggg                                                                                                                                          <  1:1809148/150‑1 (MQ=255)
        tGAGTCCAGGCGCCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCCGCATGCCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAACACCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCggggg                                                                                                                                         <  2:1025312/150‑1 (MQ=255)
           gTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGAc                                                                                                                                      >  1:438125/1‑150 (MQ=255)
            tCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTGAAAGCCggg                                                                                                                                           <  1:2269357/144‑1 (MQ=255)
            tCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTGAAAGCCgga                                                                                                                                           >  2:2269357/1‑143 (MQ=255)
              cAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGAcgcg                                                                                                                                   <  2:438125/150‑1 (MQ=255)
               aGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGTCTTTCAAGCCGTGGGGGCGCgg                                                                                                                                  >  2:737495/1‑150 (MQ=255)
               aGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCgg                                                                                                                                  >  2:1535628/1‑150 (MQ=255)
               aGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCgg                                                                                                                                  >  1:861874/1‑150 (MQ=255)
                ggCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCggg                                                                                                                                 <  1:320492/150‑1 (MQ=255)
                ggCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCggg                                                                                                                                 <  2:1514247/150‑1 (MQ=255)
                 gCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCgggg                                                                                                                                >  2:416851/1‑150 (MQ=255)
                  cACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGt                                                                                                                               <  2:1244864/150‑1 (MQ=255)
                   aCCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGAcggcgggag                                                                                                                              >  2:1014281/1‑143 (MQ=255)
                     cTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGcc                                                                                                                            >  2:1845290/1‑150 (MQ=255)
                     cTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGcc                                                                                                                            >  1:2183697/1‑150 (MQ=255)
                           cAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATAtc                                                                                                                      <  1:1889661/150‑1 (MQ=255)
                             gCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATAtcgt                                                                                                                    >  1:553065/1‑150 (MQ=255)
                              cTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGTTCCGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATAtcgtc                                                                                                                   <  1:737495/150‑1 (MQ=255)
                                      gCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCCTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGgcgc                                                                                                           <  2:774062/150‑1 (MQ=255)
                                       cccGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGcc                                                                                                                            >  1:1042953/1‑132 (MQ=255)
                                                atcatcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGtgtg                                                                                                 >  1:971970/1‑150 (MQ=255)
                                                 tcatcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGc                                                                                                <  2:2006298/150‑1 (MQ=255)
                                                 tcatcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGc                                                                                                <  1:1578131/150‑1 (MQ=255)
                                                  catcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGata                                                                                                                        <  2:1784509/125‑1 (MQ=255)
                                                  catcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGata                                                                                                                        >  1:1784509/1‑125 (MQ=255)
                                                  catcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCg                                                                                               >  2:2085882/1‑150 (MQ=255)
                                                   atcGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGc                                                                                                <  2:879580/148‑1 (MQ=255)
                                                         gccAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGTCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCAc                                                                                        <  2:757315/150‑1 (MQ=255)
                                                           cAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTGAAAGCCgga                                                                                                                                           >  2:2201530/1‑96 (MQ=255)
                                                                 cATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGa                                                                                <  1:793680/150‑1 (MQ=255)
                                                                    ggCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGAtgt                                                                             <  1:2376032/150‑1 (MQ=255)
                                                                      cGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGc                                                                           <  1:2085882/150‑1 (MQ=255)
                                                                      cGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGc                                                                           <  1:1847412/150‑1 (MQ=255)
                                                                      cGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGc                                                                           <  1:2027998/150‑1 (MQ=255)
                                                                          cggcggACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCCCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTAt                                                                       >  1:788914/1‑150 (MQ=255)
                                                                            gcggACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATcc                                                                     <  1:823632/150‑1 (MQ=255)
                                                                             cggACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCggg                                                                                                                                 <  1:1278038/89‑1 (MQ=255)
                                                                              ggACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGt                                                                   >  1:1095903/1‑150 (MQ=255)
                                                                                        ttCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCcaca                                                                                                      >  2:707010/1‑105 (MQ=255)
                                                                                        ttCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGc                                                         >  2:1610355/1‑150 (MQ=255)
                                                                                         tCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCgggg                                                                                                                                          >  2:292325/1‑68 (MQ=255)
                                                                                         tCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGc                                                         <  1:586801/149‑1 (MQ=255)
                                                                                         tCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGc                                                         >  2:586801/1‑149 (MQ=255)
                                                                                          caccacCTGAAGGCACTCGTCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGAGGGCGCCCACNGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCAt                                                       <  1:693524/150‑1 (MQ=255)
                                                                                          caccacCGGAAGGCACTGGCCCCAAACCCGGGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACGGTGTGCGGTTGCTGGTCGAGGATTCGCTTATCCGGGCGCAGGTGCAt                                                       <  2:1234302/150‑1 (MQ=255)
                                                                                           accaccTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCACACAGTGAGCGGTTGCACGGCGCGGATGTGCCTATCCGTGCACAGGTGccgg                                                      >  1:138220/1‑147 (MQ=255)
                                                                                            ccaccTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGc                                                     >  2:894379/1‑150 (MQ=255)
                                                                                            ccaccTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGAAGCGGGGAGGAGATATCGTCCCCGCGGCGCATTTTGTTCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGc                                                     <  1:1498452/150‑1 (MQ=255)
                                                                                             caccTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGAt                                                                               <  1:381320/123‑1 (MQ=255)
                                                                                                cTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATg                                                 >  2:181022/1‑150 (MQ=255)
                                                                                                  gAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGCTGTACGCAGCCGTGCACAGGTGCATGCGATGga                                               <  1:1871845/150‑1 (MQ=255)
                                                                                                  gAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGga                                               <  1:1518060/150‑1 (MQ=255)
                                                                                                  gAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGga                                               >  2:2387442/1‑150 (MQ=255)
                                                                                                     ggCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGAGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGaa                                            <  1:1375656/150‑1 (MQ=255)
                                                                                                      gCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGCTGGAGGAt                                           >  1:1006311/1‑150 (MQ=255)
                                                                                                       cACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGTCTAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATc                                          <  1:2224101/150‑1 (MQ=255)
                                                                                                       cACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATc                                          <  2:1198694/150‑1 (MQ=255)
                                                                                                       cACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATc                                          <  1:513638/150‑1 (MQ=255)
                                                                                                        aCTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAgtgt                                                                                                  >  2:547432/1‑93 (MQ=255)
                                                                                                         cTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAg                                                                                                     >  2:1301849/1‑89 (MQ=255)
                                                                                                             ccccAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTg                                    <  1:2387442/150‑1 (MQ=255)
                                                                                                              cccAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTgc                                   >  1:979945/1‑150 (MQ=255)
                                                                                                              cccAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTgc                                   >  2:2378189/1‑150 (MQ=255)
                                                                                                               ccAAACCCGCGCAACAGCATTCTGTGCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTgcg                                  >  1:933406/1‑150 (MQ=255)
                                                                                                                    cccGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCAAGc                             >  1:1314829/1‑150 (MQ=255)
                                                                                                                     ccGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGcc                            >  2:2168879/1‑150 (MQ=255)
                                                                                                                     ccGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACACGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCt                                         <  1:1944175/137‑1 (MQ=255)
                                                                                                                     ccGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACACGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAAACt                                         >  2:1944175/1‑137 (MQ=255)
                                                                                                                      cgcgCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGAtgt                                                                             <  1:523870/100‑1 (MQ=255)
                                                                                                                        cgcAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGAt                         <  1:2168879/150‑1 (MQ=255)
                                                                                                                           aaCAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGGATGCGATGGAGAATCTTTCTGCGCACGCCGATGCt                      >  2:1091671/1‑150 (MQ=255)
                                                                                                                           aaCAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCt                      >  2:389238/1‑150 (MQ=255)
                                                                                                                              aGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTCTCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCtgat                   >  1:2303941/1‑150 (MQ=255)
                                                                                                                              aGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCtgat                   <  1:1219076/150‑1 (MQ=255)
                                                                                                                                 aTTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGga                                               <  1:810822/119‑1 (MQ=255)
                                                                                                                                 aTTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGga                                               >  2:810822/1‑119 (MQ=255)
                                                                                                                                 aTTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGaa                >  2:609496/1‑150 (MQ=255)
                                                                                                                                  tgcTGTTCTCCGGCTTTCAAGCAGGGGGGCCGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGCTGcaa               >  2:1055636/3‑150 (MQ=255)
                                                                                                                                  cccTGTCCTCCGGCCGGCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCGACTGTGGGCGGTTGCACGGCTAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGTATCTTTCTGCGCACGCCGACGCTGATGaaa               <  2:138220/148‑1 (MQ=255)
                                                                                                                                     tGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAg                                                             <  2:268009/101‑1 (MQ=255)
                                                                                                                                      gTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCAt           <  1:416851/150‑1 (MQ=255)
                                                                                                                                       ttCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATg          >  2:1410215/1‑150 (MQ=255)
                                                                                                                                       ttCTCCGACTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATg          <  1:1088580/150‑1 (MQ=255)
                                                                                                                                        cccccGGCTTGAAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATg                                                 >  1:71670/4‑110 (MQ=255)
                                                                                                                                         ccccGGCTTGAAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATc                                          <  2:94752/114‑1 (MQ=255)
                                                                                                                                         ccccGGCTTGAAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATc                                          >  1:94752/3‑116 (MQ=255)
                                                                                                                                         ccccGGCTTGAAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGCAATCATGGa        >  2:1499089/3‑150 (MQ=255)
                                                                                                                                          tcCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGAt                                                  <  1:2317397/107‑1 (MQ=255)
                                                                                                                                          tcCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGAt                                                  >  2:2317397/1‑107 (MQ=255)
                                                                                                                                            cGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATc                                          <  2:401363/113‑1 (MQ=255)
                                                                                                                                             ggCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGAATgg    >  1:2049619/1‑150 (MQ=255)
                                                                                                                                             ggCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGAATgg    >  1:274913/1‑150 (MQ=255)
                                                                                                                                              gCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCCAATGAAATCATGGAATGGt   >  1:124024/1‑150 (MQ=255)
                                                                                                                                               cTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGAATGGtt  <  1:1610355/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACGGTCGATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGAATGGTT  >  NC_002947/3726312‑3726604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑