BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,812,708	C→T	14.2%	A330V (GCC→GTC)	PP_3368 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,812,708	0	C	T	14.2%	252.2 / 31.2	106	A330V (GCC→GTC)	PP_3368	MFS transporter
Reads supporting (aligned to +/- strand): ref base C (54/37); new base T (11/4); total (65/41)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01

Only 100 of 109 total aligned reads displayed.

CGTGGGCTTGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACTGCGCTGGTT  >  NC_002947/3812559‑3812856

cGTGGGCTTGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGc                                                                                                                                                      <  2:2077534/150‑1 (MQ=255)

      cTTGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGa                                                                                                                                                <  2:1115422/150‑1 (MQ=255)

       ttGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGTACATTGcc                                                                                                                                                     >  1:1696270/1‑144 (MQ=255)

       ttGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGcc                                                                                                                                                     <  2:1696270/144‑1 (MQ=255)

       ttGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAg                                                                                                                                               >  1:1164019/1‑150 (MQ=255)

         ggaaCTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGgc                                                                                                                                             <  1:257432/147‑1 (MQ=255)

         ggTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGGCAATGTCCATGAGgc                                                                                                                                             <  2:734113/150‑1 (MQ=255)

            aCTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTCCCTTCGCCACAAAGTCTTTGTGTAGCCTGCGGCTGGGCATAGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGcc                                                                                                                                          <  1:1740921/150‑1 (MQ=255)

             cTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGACCGCCTTGGCGGCACCGCTACA‑CATCCGCCTCATGGACATTGCCCATGAGGCGCCt                                                                                                                                         <  1:1316545/150‑1 (MQ=255)

                aTGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGAACCATGATCGCCTTCGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCTCCTAGc                                                                                                                                      >  2:148161/1‑150 (MQ=255)

                  gtgGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCt                                                                                                                                    <  2:1973282/150‑1 (MQ=255)

                    ggtcggtcGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTgg                                                                                                                                  <  2:1164019/150‑1 (MQ=255)

                     gtcggtcgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCGTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGc                                                                                                                                 >  1:18745/1‑150 (MQ=255)

                     gtcggtcgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGc                                                                                                                                 >  1:548832/1‑150 (MQ=255)

                      tcggtcgCCGTGCTGCTGTTCTTTACCTTCGCAGCGCGGTCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCa                                                                                                                                >  1:1667008/1‑150 (MQ=255)

                          tcgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAgcgg                                                                                                                            >  1:2232035/1‑150 (MQ=255)

                           cgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAgcggc                                                                                                                           <  2:1067409/150‑1 (MQ=255)

                           cgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCGGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAgcggc                                                                                                                           >  2:2153424/1‑150 (MQ=255)

                              cGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGa                                                                                                                                                <  1:257481/126‑1 (MQ=255)

                              cGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGa                                                                                                                                                >  2:257481/1‑126 (MQ=255)

                               gTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTcc                                                                                                                       <  1:1139015/150‑1 (MQ=255)

                                  ctgctgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCTCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAAc                                                                                                                    <  2:287669/150‑1 (MQ=255)

                                  ctgctgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAAc                                                                                                                    <  2:548832/150‑1 (MQ=255)

                                   tgctgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTg                                                                                                                                   <  2:1452796/134‑1 (MQ=255)

                                   tgctgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTg                                                                                                                                   >  1:1452796/1‑134 (MQ=255)

                                    gctgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCa                                                                                                                  >  2:393228/1‑150 (MQ=255)

                                      tgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATg                                                                                                                >  1:2095924/1‑150 (MQ=255)

                                       gTTCTTTACCTTCGCCGCCCGGGCGCTGTGGAGCTTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgc                                                                                                               <  2:1053733/150‑1 (MQ=255)

                                       gTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGGCAATGTCCATGa                                                                                                                                                <  1:2082188/117‑1 (MQ=255)

                                       gTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGGCAATGTCCATGa                                                                                                                                                >  2:2082188/1‑117 (MQ=255)

                                       gTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgc                                                                                                               <  2:2016315/150‑1 (MQ=255)

                                        ttCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgcg                                                                                                              <  2:18745/150‑1 (MQ=255)

                                               ccTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGGCAATGTCCATGAGgcggat                                                                                                                                         <  2:1604579/116‑4 (MQ=39)

                                                 ttCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAAc                                                                                                     >  2:489555/1‑150 (MQ=255)

                                                 ttCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATAGGCTTGGTGACTAGCATGACCTCCTTGGCGGCTCCTCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAAc                                                                                                     >  1:1252537/1‑150 (MQ=255)

                                                        cccAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCa                                                                                              <  2:1667008/150‑1 (MQ=255)

                                                                cTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTg                                                                                      <  2:2095924/150‑1 (MQ=255)

                                                                  gtgGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTggg                                                                                    <  1:353401/150‑1 (MQ=255)

                                                                           gctgctgGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCt                                                                           >  1:1943183/1‑150 (MQ=255)

                                                                             tgctgGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTgg                                                                         <  2:1498504/150‑1 (MQ=255)

                                                                             tgctgGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGACTGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGACGTGGCTgg                                                                         >  2:1053846/1‑150 (MQ=255)

                                                                               ctgGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGa                                                                       <  1:2081669/150‑1 (MQ=255)

                                                                               ctgGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGa                                                                       <  2:25546/150‑1 (MQ=255)

                                                                                tgGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGccc                                                                                        <  2:1777748/132‑1 (MQ=255)

                                                                                tgGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGccc                                                                                        >  1:1777748/1‑132 (MQ=255)

                                                                                   gCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCa                                                                   <  1:343178/150‑1 (MQ=255)

                                                                                    cATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCAt                                                                  <  1:1203100/150‑1 (MQ=255)

                                                                                        ggCCTGGTGGGCACCATGAACGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCg                                                              >  2:640717/1‑150 (MQ=255)

                                                                                         gCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATgg                                                                <  1:374949/147‑1 (MQ=255)

                                                                                         gCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATgg                                                                >  2:374949/1‑147 (MQ=255)

                                                                                           cTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATc                                                           <  1:1663691/150‑1 (MQ=255)

                                                                                              gtggGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACt                                                        >  2:251516/1‑150 (MQ=255)

                                                                                                cggCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGc                                                      >  2:1658139/2‑150 (MQ=255)

                                                                                                 ggCACCATGATCACCTACGCCACACAGC‑ACACGCTCCGCCTACAGGACATTGCTCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGCTCACtcca                                                     <  2:737194/150‑4 (MQ=255)

                                                                                                    aCCATGATCGCCGTGGCGGCACCGCGACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGc                                                  >  2:1354608/1‑150 (MQ=255)

                                                                                                      cATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGc                                                                                                                                 >  1:1744820/1‑69 (MQ=255)

                                                                                                      cATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGc                                                                                                                                 <  2:1744820/69‑1 (MQ=255)

                                                                                                      cATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCAt                                                >  1:355846/1‑150 (MQ=255)

                                                                                                      cATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCAt                                                >  1:354194/1‑150 (MQ=255)

                                                                                                         gATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATggg                                             >  1:1129694/1‑150 (MQ=255)

                                                                                                           tCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCt                                           >  2:1018907/1‑150 (MQ=255)

                                                                                                               cTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGAc                                       >  2:368828/1‑150 (MQ=255)

                                                                                                               cTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGCCGCCGAACCTGCCAGCGGCGTCCAACCATTCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCCGGGAGGCATGGCGATCACTGCCGGCGTGGGCTGGAc                                       >  2:368788/1‑150 (MQ=255)

                                                                                                                  ggcggcACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAg                                    >  2:672214/1‑150 (MQ=255)

                                                                                                                        aCCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAAACTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCgg                              >  1:549470/1‑150 (MQ=255)

                                                                                                                            cTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGa                             >  2:568395/1‑147 (MQ=255)

                                                                                                                            cTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGa                             <  1:568395/147‑1 (MQ=255)

                                                                                                                              aCA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACAt                        >  2:345855/1‑150 (MQ=255)

                                                                                                                                a‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCg                      >  1:2172920/1‑150 (MQ=255)

                                                                                                                                   aTCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGgc                    >  1:1937769/1‑150 (MQ=255)

                                                                                                                                   aTCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGgc                    >  1:1117806/1‑150 (MQ=255)

                                                                                                                                     ccGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGgcgc                  >  1:1596565/1‑150 (MQ=255)

                                                                                                                                       gCCTCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGa                             <  2:595888/137‑1 (MQ=255)

                                                                                                                                       gCCTCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGa                             >  1:595888/1‑137 (MQ=255)

                                                                                                                                       gCCTCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGcgccg                >  1:1692002/1‑150 (MQ=255)

                                                                                                                                         cTCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCcgcg              >  1:521940/1‑150 (MQ=255)

                                                                                                                                          tCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCACTGCCCTGGGGCCTTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGa             >  2:1891003/1‑150 (MQ=255)

                                                                                                                                          tCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGa             >  2:726903/1‑150 (MQ=255)

                                                                                                                                          tCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGa             >  1:2220611/1‑150 (MQ=255)

                                                                                                                                          tCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGa             >  1:1632612/1‑150 (MQ=255)

                                                                                                                                          tCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGAACAGCACCGGATACATCGGCGCCGCGa             >  1:383036/1‑150 (MQ=255)

                                                                                                                                          tCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTgg                                                                         <  2:379241/90‑1 (MQ=255)

                                                                                                                                          tCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTgg                                                                         >  1:379241/1‑90 (MQ=255)

                                                                                                                                            aTGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGc                                                                    <  1:2109530/93‑1 (MQ=255)

                                                                                                                                            aTGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACt           >  2:1466027/1‑150 (MQ=255)

                                                                                                                                            aTGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACt           >  1:498665/1‑150 (MQ=255)

                                                                                                                                            aTGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACt           >  2:1566934/1‑150 (MQ=255)

                                                                                                                                            aTGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGACAATCACTGCGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACt           >  1:1852233/1‑150 (MQ=255)

                                                                                                                                            aTGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCAGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACt           >  1:1999884/1‑150 (MQ=255)

                                                                                                                                             tGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACTg          >  1:144095/1‑150 (MQ=255)

                                                                                                                                             tGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACTg          >  2:1373074/1‑150 (MQ=255)

                                                                                                                                              ggACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGcgccgc               <  1:706907/144‑1 (MQ=255)

                                                                                                                                              ggACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGcgccgc               >  2:706907/1‑144 (MQ=255)

                                                                                                                                               gACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGgc                    >  1:163606/1‑138 (MQ=255)

                                                                                                                                               gACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGgc                    <  2:163606/138‑1 (MQ=255)

                                                                                                                                                aCATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACTgcgc       <  1:1466027/150‑1 (MQ=255)

                                                                                                                                                  aTTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGa                                                                       <  2:234776/84‑1 (MQ=255)

                                                                                                                                                  aTTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGa                                                                       >  1:234776/1‑84 (MQ=255)

                                                                                                                                                     gCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACTGCGCTGGtt  >  2:1863745/1‑150 (MQ=255)

                                                                                                                                                     gCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACTGCGCTGGtt  <  1:118148/150‑1 (MQ=255)

CGTGGGCTTGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACA‑GATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGACTGCGCTGGTT  >  NC_002947/3812559‑3812856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`