Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,018,109 A→T 5.2% Q167L (CAG→CTG)  PP_3545 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,018,1090AT 5.2% 423.0 / 10.6 135Q167L (CAG→CTG) PP_3545two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base A (62/64);  new base T (3/4);  total (65/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 137 total aligned reads displayed.

GACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGACACCACACAGCTGCTTGAAC  >  NC_002947/4017961‑4018258
                                                                                                                                                    |                                                                                                                                                     
gacgacGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAg                                                                                                                                                      >  1:2018109/1‑150 (MQ=255)
   gacgCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCTGgcg                                                                                                                                                   <  1:1533478/150‑1 (MQ=255)
   gacgCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGgcg                                                                                                                                                   >  1:1039278/1‑150 (MQ=255)
   gacgCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGgcg                                                                                                                                                   >  1:336707/1‑150 (MQ=255)
     cgCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCa                                                                                                                                                 <  1:2318023/150‑1 (MQ=255)
     cgCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCa                                                                                                                                                 >  1:400171/1‑150 (MQ=255)
      gCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCag                                                                                                                                                >  2:1965084/1‑150 (MQ=255)
        accacGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCagaa                                                                                                                                              >  2:191099/1‑150 (MQ=255)
        accacGCCACTACGAAATGCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCagaa                                                                                                                                              <  1:1544638/150‑1 (MQ=255)
           acGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGAt                                                                                                                                           <  2:1509649/150‑1 (MQ=255)
            cGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATg                                                                                                                                          <  2:1828589/150‑1 (MQ=255)
             gCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATgg                                                                                                                                         <  2:1036545/150‑1 (MQ=255)
             gCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATgg                                                                                                                                         <  1:1417547/150‑1 (MQ=255)
               cACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAg                                                                                                                                       <  2:1580405/150‑1 (MQ=255)
                aCTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAgg                                                                                                                                      <  1:1675075/150‑1 (MQ=255)
                aCTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAgg                                                                                                                                      >  2:2342705/1‑150 (MQ=255)
                  tACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCg                                                                                                                                    >  1:2364080/1‑150 (MQ=255)
                  tACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCg                                                                                                                                    >  2:452139/1‑150 (MQ=255)
                   aCGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCgg                                                                                                                                   >  1:458165/1‑150 (MQ=255)
                     gAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTc                                                                                                                                 >  1:2321112/1‑150 (MQ=255)
                        aTTCGCCACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGc                                                                                                                              <  1:1334009/150‑1 (MQ=255)
                         ttCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGcc                                                                                                                             >  2:1287793/1‑150 (MQ=255)
                         ttCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGAAGCAGGCAGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGAGGGCGGcc                                                                                                                             >  1:1131066/1‑150 (MQ=255)
                          tCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCa                                                                                                                            <  2:458165/150‑1 (MQ=255)
                          tCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGAGTGACCGAACAGGAAAGCCTGCAGCAGGCCGAAGAAGCACTGCCACAGGCGCAGAAGATGGAGGCGGTGGGCCa                                                                                                                            >  1:506882/1‑150 (MQ=255)
                             cTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGtt                                                                                                                         <  1:105880/150‑1 (MQ=255)
                               accaccCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGa                                                                                                                       >  1:882316/1‑150 (MQ=255)
                                  accCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACcg                                                                                                                    <  2:2203363/150‑1 (MQ=255)
                                    ccGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACcggc                                                                                                                  >  1:799461/1‑150 (MQ=255)
                                    ccGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACcggc                                                                                                                  <  1:1599265/150‑1 (MQ=255)
                                      gTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAg                                                                                                                                       <  1:1501451/127‑1 (MQ=255)
                                          cTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGAtt                                                                                                            >  2:2099406/1‑150 (MQ=255)
                                           tCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGCGGTCGGTGGGCCAGTTGACCGGCGGGATTg                                                                                                           >  1:1811445/1‑150 (MQ=255)
                                               tGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCa                                                                                                       <  1:861041/150‑1 (MQ=255)
                                                    agcagcGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGAtt                                                                                                  <  1:1916845/150‑1 (MQ=255)
                                                        gcGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGAGCACGAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTcaa                                                                                              >  1:1955316/1‑150 (MQ=255)
                                                        gcGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGAGTCGACGTACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTcaa                                                                                              <  1:1351707/150‑1 (MQ=255)
                                                         cGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACTCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTcaac                                                                                             >  1:1676668/1‑150 (MQ=255)
                                                             ttCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGc                                                                                                                              >  1:1089689/1‑113 (MQ=255)
                                                             ttCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGc                                                                                                                              <  2:1089689/113‑1 (MQ=255)
                                                             ttCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCAt                                                                                                      >  2:144469/1‑137 (MQ=255)
                                                                aGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTc                                                                                                                                 <  1:371356/107‑1 (MQ=255)
                                                                aGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTc                                                                                                                                 >  2:371356/1‑107 (MQ=255)
                                                                 gggCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGACCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACctgct                                                                                     >  1:76528/1‑150 (MQ=255)
                                                                      gcTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCa                                                                                <  1:452139/150‑1 (MQ=255)
                                                                        tACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGGACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGc                                                                              >  1:194605/1‑150 (MQ=255)
                                                                         aCCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGccc                                                                                                        >  1:1182551/1‑123 (MQ=255)
                                                                         aCCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGccc                                                                                                        <  2:1182551/123‑1 (MQ=255)
                                                                         aCCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCa                                                                             >  2:1704438/1‑150 (MQ=255)
                                                                          ccTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCAt                                                                            >  2:917276/1‑150 (MQ=255)
                                                                            tGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGGAGCAGGCCGAAGAACCGCTGCGGCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATcc                                                                          <  2:74032/150‑1 (MQ=255)
                                                                            tGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATcc                                                                          >  1:2334286/1‑150 (MQ=255)
                                                                                 gCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGtt                                                                                                                         <  1:246042/98‑1 (MQ=255)
                                                                                 gCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGtt                                                                                                                         >  2:246042/1‑98 (MQ=255)
                                                                                  ccTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCg                                                                                                                                    <  1:387163/86‑1 (MQ=255)
                                                                                  ccTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCg                                                                                                                                    >  2:387163/1‑86 (MQ=255)
                                                                                  ccTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGgcg                                                                    >  1:493904/1‑150 (MQ=255)
                                                                                    tATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGa                                                                                                              <  2:221773/106‑1 (MQ=255)
                                                                                    tATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGa                                                                                                              >  1:221773/1‑106 (MQ=255)
                                                                                    tATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGgcgcg                                                                  >  2:2335224/1‑150 (MQ=255)
                                                                                           tCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACcggc                                                                                                                  <  2:1325780/95‑1 (MQ=255)
                                                                                           tCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACcggc                                                                                                                  >  1:1325780/1‑95 (MQ=255)
                                                                                             aCGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTg                                                         <  2:2184402/150‑1 (MQ=255)
                                                                                             aCGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTg                                                         <  2:2364080/150‑1 (MQ=255)
                                                                                               gCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATgg                                                                                                                                         >  2:1489265/1‑68 (MQ=255)
                                                                                               gCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATgg                                                                                                                                         <  1:1489265/68‑1 (MQ=255)
                                                                                               gCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGc                                                       >  1:1459000/1‑150 (MQ=255)
                                                                                               gCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGc                                                       <  2:37195/150‑1 (MQ=255)
                                                                                                 aGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCGCGCATCCTTGGCGCGCTGGAACTGGCCg                                                     >  1:1811596/1‑150 (MQ=255)
                                                                                                 aGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGACAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTTCCCATTATTTTAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCg                                                     <  2:882316/150‑1 (MQ=255)
                                                                                                  gcgcGTGACCGAACAGGACCGCCTGCAGCAGGCCGAAGAAGCACTGCGCCCGGCGCAGAAGATCGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCgg                                                    <  2:735949/150‑1 (MQ=255)
                                                                                                         aCCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGt                                             <  2:1003371/150‑1 (MQ=255)
                                                                                                           cGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTgg                                                              <  2:1407793/131‑1 (MQ=255)
                                                                                                           cGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTgg                                                              >  1:1407793/1‑131 (MQ=255)
                                                                                                           cGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCt                                           >  1:1013235/1‑150 (MQ=255)
                                                                                                            gaccAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCACTGGAACTGGCCGGGCAACGTCTg                                          >  2:703589/4‑150 (MQ=255)
                                                                                                            gAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACcggcgg                                                                                                                >  2:1087333/1‑80 (MQ=255)
                                                                                                               cAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTg                                       <  1:406218/150‑1 (MQ=255)
                                                                                                                  gAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGAGCTGGAACTGGCCGCGCAACGTCTGGTgcag                                    <  2:1955316/150‑1 (MQ=255)
                                                                                                                    ccGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATGCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTgcagca                                  >  2:1648813/2‑150 (MQ=255)
                                                                                                                      gCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAAc                                <  1:2261441/150‑1 (MQ=255)
                                                                                                                          gcagcaGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCt                            >  2:1008467/1‑150 (MQ=255)
                                                                                                                            agcagGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCgg                                                    <  1:831016/124‑1 (MQ=255)
                                                                                                                                  ccGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGaca                    <  2:493904/150‑1 (MQ=255)
                                                                                                                                   cGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGacac                   >  2:1896151/1‑150 (MQ=255)
                                                                                                                                   cGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTCCCCACGATTCCGAACCGCTGCTGGCCAGCAGCCTTGGCGCTCTGGAACTGGCCGGGCAACGTCTGGGGCAGCAACGCCTGGCGGacac                   <  1:2099406/150‑1 (MQ=255)
                                                                                                                                     aagaagCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGAcacca                 <  2:1811596/150‑1 (MQ=255)
                                                                                                                                        aagCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGc                         <  1:2361332/139‑1 (MQ=255)
                                                                                                                                        aagCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGc                         >  2:2361332/1‑139 (MQ=255)
                                                                                                                                         tcttcTGCGCCTGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGACACcacaca             >  2:2208750/5‑150 (MQ=255)
                                                                                                                                         tcttcTGCGCCTGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGACACcacaca             >  1:469382/5‑150 (MQ=255)
                                                                                                                                           cACTGCGCCAGGCGCAGAAGATGGGGGAGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGACACCACACAgc           <  2:1507800/150‑1 (MQ=255)
                                                                                                                                            aCTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCGATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGACACCACACAgct          >  2:776487/1‑150 (MQ=255)
                                                                                                                                            aCTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCt                                           >  1:1828949/1‑117 (MQ=255)
                                                                                                                                              tGCGCCTGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGACACCACACAgctgc        <  2:469382/150‑1 (MQ=255)
                                                                                                                                               gcgcCTGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGt                                        >  2:2049383/1‑117 (MQ=255)
                                                                                                                                               gcgcCTGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGt                                        <  1:2049383/117‑1 (MQ=255)
                                                                                                                                                cgcCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGACACCACACAGCTGCtt      <  2:1811445/150‑1 (MQ=255)
                                                                                                                                                cgcCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCACTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGACACCACACAGCTGCtt      <  1:703589/150‑1 (MQ=255)
                                                                                                                                                    aGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGACACCACACAGCTGCTTGAAc  <  2:2018109/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCGATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGGCCGGGCAACGTCTGGTGCAGCAACGCCTGGCGGACACCACACAGCTGCTTGAAC  >  NC_002947/4017961‑4018258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑