BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,301,102	A→G	6.9%	T75A (ACG→GCG)	PP_3774 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,301,102	0	A	G	6.9%	304.6 / 8.9	102	T75A (ACG→GCG)	PP_3774	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (46/49); new base G (3/4); total (49/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

Only 100 of 104 total aligned reads displayed.

ACGGTGCTGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCA  >  NC_002947/4300959‑4301249

aCGGTGCTGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTgg                                                                                                                                                >  2:883493/1‑150 (MQ=255)

 cGGTGCTGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGc                                                                                                                                               <  1:1118863/150‑1 (MQ=255)

     gCTGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAgc                                                                                                                                           <  1:1473149/150‑1 (MQ=255)

      cTGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTg                                                                                                                                                 >  2:1780291/1‑143 (MQ=255)

      cTGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTg                                                                                                                                                 <  1:1780291/143‑1 (MQ=255)

       tGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGa                                                                                                                                                      <  1:1930615/137‑1 (MQ=255)

       tGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGa                                                                                                                                                      >  2:1930615/1‑137 (MQ=255)

          aGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCa                                                                                                                                      <  2:1032182/150‑1 (MQ=255)

           gctaTTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCTTTCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAg                                                                                                                                     <  1:1122086/147‑1 (MQ=255)

              aTTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGTACCAATGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGAt                                                                                                                                  >  1:761601/1‑150 (MQ=255)

              aTTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGAt                                                                                                                                  >  1:847246/1‑150 (MQ=255)

                  cccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAgtcttgcg                                                                                                                              <  1:883493/150‑8 (MQ=255)

                  cccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                              >  1:514533/1‑150 (MQ=255)

                  cccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                              <  1:784702/150‑1 (MQ=255)

                   ccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCa                                                                                                                                >  1:743685/1‑147 (MQ=255)

                   ccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCa                                                                                                                                <  2:743685/147‑1 (MQ=255)

                     gCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGa                                                                                                                           <  2:291979/150‑1 (MQ=255)

                          ctgaGCACCGTCACGATGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGc                                                                                                                      >  1:2199974/4‑150 (MQ=255)

                           ccAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGcc                                                                                                                     <  2:430244/150‑1 (MQ=255)

                            cAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCa                                                                                                                    >  2:158220/1‑150 (MQ=255)

                            cAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCa                                                                                                                    >  1:2137080/1‑150 (MQ=255)

                                 tCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACaa                                                                                                               <  2:514533/150‑1 (MQ=255)

                                 tCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACaa                                                                                                               <  2:1921014/150‑1 (MQ=255)

                                      aCGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACg                                                                                                          <  1:1301218/150‑1 (MQ=255)

                                                    tGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCaa                                                                                            <  2:259182/150‑1 (MQ=255)

                                                       cTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTc                                                                                         >  1:707244/1‑150 (MQ=255)

                                                       cTCGATCAAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTc                                                                                         >  1:1532736/1‑150 (MQ=255)

                                                        tCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTg                                                                                                                             >  2:1903043/1‑113 (MQ=255)

                                                        tCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTg                                                                                                                             <  1:1903043/113‑1 (MQ=255)

                                                        tCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCg                                                                                        >  2:1903270/1‑150 (MQ=255)

                                                        tCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCg                                                                                        >  1:819497/1‑150 (MQ=255)

                                                          gATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGAACAAGCTCAGCTCGgc                                                                                      <  2:1799656/150‑1 (MQ=255)

                                                           aTCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGgcg                                                                                     <  2:707244/150‑1 (MQ=255)

                                                           aTCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGgcg                                                                                     >  2:2171038/1‑150 (MQ=255)

                                                               aGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATc                                                                                                                        >  2:1908431/1‑111 (MQ=255)

                                                               aGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATc                                                                                                                        <  1:1908431/111‑1 (MQ=255)

                                                                 ttCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAg                                                                              >  1:450734/1‑150 (MQ=255)

                                                                    tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTg                                                                                                                                                 >  1:2086810/1‑81 (MQ=255)

                                                                    tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTg                                                                                                                                                 <  2:2086810/81‑1 (MQ=255)

                                                                    tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTg                                                                                                                                                 <  2:2086768/81‑1 (MQ=255)

                                                                    tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTg                                                                                                                                                 >  1:2086768/1‑81 (MQ=255)

                                                                    tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAggtg                                                                           >  2:1786591/1‑150 (MQ=255)

                                                                    tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAggtg                                                                           <  1:1028671/150‑1 (MQ=255)

                                                                        ccccAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACAAGGTGCGAGTGCATGGAAACGCAAGACTGTACGTTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCa                                                                       >  1:1253232/1‑150 (MQ=255)

                                                                         cccAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCAt                                                                      >  1:1631436/1‑150 (MQ=255)

                                                                            aaccaaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTg                                                                                                                                                 <  1:1628631/73‑1 (MQ=255)

                                                                            aaccaaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTg                                                                                                                                                 >  2:1628631/1‑73 (MQ=255)

                                                                            aaccaaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGc                                                                                                                                               <  1:417080/75‑1 (MQ=255)

                                                                            aaccaaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGc                                                                                                                                               >  2:417080/1‑75 (MQ=255)

                                                                             accaaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGcc                                                                  <  2:1161793/150‑1 (MQ=255)

                                                                               caaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCa                                                                <  2:497008/150‑1 (MQ=255)

                                                                               caaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCa                                                                <  2:120588/150‑1 (MQ=255)

                                                                                 aaGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGa                                                              <  1:804714/150‑1 (MQ=255)

                                                                                  tggggCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGAt                                                             <  2:357306/149‑1 (MQ=255)

                                                                                    gggCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGc                                                           >  2:121110/1‑150 (MQ=255)

                                                                                    gggCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGc                                                           <  2:450734/150‑1 (MQ=255)

                                                                                        ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGAc                                                                                                           >  2:1958338/1‑99 (MQ=255)

                                                                                        ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGAc                                                                                                           <  1:1958338/99‑1 (MQ=255)

                                                                                        ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGt                                                       >  1:1905419/1‑150 (MQ=255)

                                                                                        ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGt                                                       >  1:245055/1‑150 (MQ=255)

                                                                                        ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGt                                                       >  1:2032630/1‑150 (MQ=255)

                                                                                            aGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTAcgc                                                   <  2:1905419/150‑1 (MQ=255)

                                                                                               aaaaGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCggg                                                <  2:2137080/150‑1 (MQ=255)

                                                                                               aaaaGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCggg                                                >  1:1556759/1‑150 (MQ=255)

                                                                                                 aaGCACCCACAGGACACGCTGCGAATGGACGGAACCCTTACACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATCACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGga                                              <  1:738078/150‑1 (MQ=255)

                                                                                                    cACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGgacga                                           <  2:2199974/150‑1 (MQ=255)

                                                                                                       ccACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAATGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACgg                                        >  2:1664396/1‑150 (MQ=255)

                                                                                                        cacaGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTc                                                                                              <  2:2030377/96‑1 (MQ=255)

                                                                                                        cacaGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGc                                       <  1:2171038/150‑1 (MQ=255)

                                                                                                         acaGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGcc                                      >  1:504428/1‑150 (MQ=255)

                                                                                                           aGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCccg                                    <  2:1556759/150‑1 (MQ=255)

                                                                                                           aGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCccg                                    >  1:336479/1‑150 (MQ=255)

                                                                                                              acacGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACg                                                                                                          >  1:225852/1‑78 (MQ=255)

                                                                                                              acacGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACg                                                                                                          <  2:225852/78‑1 (MQ=255)

                                                                                                                   cTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTaaa                            <  2:1631436/150‑1 (MQ=255)

                                                                                                                      cGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGgt                         <  1:121110/150‑1 (MQ=255)

                                                                                                                        aaTGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGAAGACGGCCCGCCGGTAAAGttgt                       <  2:761601/150‑1 (MQ=255)

                                                                                                                         aTGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGtt                      >  2:1750015/1‑150 (MQ=255)

                                                                                                                           ggACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGCCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGAGGGCCCGCCGGTAAAGGTGTTCt                    >  1:1931790/1‑150 (MQ=255)

                                                                                                                            gACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCCCGACGGTGG‑CATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTg                   <  2:1532736/150‑1 (MQ=255)

                                                                                                                            gACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTg                   <  2:819497/150‑1 (MQ=255)

                                                                                                                            gACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTg                   >  2:293767/1‑150 (MQ=255)

                                                                                                                             aCGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTgg                  <  2:2032630/150‑1 (MQ=255)

                                                                                                                                  aaCGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACaa             <  1:1786591/150‑1 (MQ=255)

                                                                                                                                   aCGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATGGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAg            <  2:2134450/150‑1 (MQ=255)

                                                                                                                                   aCGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAg            <  2:847246/150‑1 (MQ=255)

                                                                                                                                    cGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAgg           <  1:158220/150‑1 (MQ=255)

                                                                                                                                    cGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAgg           >  1:608282/1‑150 (MQ=255)

                                                                                                                                      cAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGacgacg                                         <  1:1689879/118‑1 (MQ=255)

                                                                                                                                      cAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGacgacg                                         >  2:1689879/1‑118 (MQ=255)

                                                                                                                                      cAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGc         >  1:608813/1‑150 (MQ=255)

                                                                                                                                      cAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGc         >  1:1412459/1‑150 (MQ=255)

                                                                                                                                       aaGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCa        >  1:524827/1‑150 (MQ=255)

                                                                                                                                       aaGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCa        >  2:1409355/1‑150 (MQ=255)

                                                                                                                                         tccTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCAc                                                         <  1:1985722/97‑1 (MQ=255)

                                                                                                                                         tccTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCAc                                                         >  2:1985722/3‑99 (MQ=255)

                                                                                                                                           cTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAg                           <  1:2303514/127‑1 (MQ=255)

                                                                                                                                           cTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAg                           >  2:2303514/1‑127 (MQ=255)

                                                                                                                                           cTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACAt    >  1:1546156/1‑150 (MQ=255)

                                                                                                                                             ggACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCa  <  1:1409355/150‑1 (MQ=255)

ACGGTGCTGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCA  >  NC_002947/4300959‑4301249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`