Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,301,115 C→T 6.4% A79V (GCC→GTC)  PP_3774 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,301,1150CT 6.4% 270.2 / 6.7 93A79V (GCC→GTC) PP_3774hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (42/45);  new base T (2/4);  total (44/49)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01

AGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTGGGGTTTCGG  >  NC_002947/4300969‑4301263
                                                                                                                                                  |                                                                                                                                                     
aGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCa                                                                                                                                                    <  2:1032182/150‑1 (MQ=255)
 gctaTTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCTTTCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAg                                                                                                                                                   <  1:1122086/147‑1 (MQ=255)
    aTTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGTACCAATGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGAt                                                                                                                                                >  1:761601/1‑150 (MQ=255)
    aTTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGAt                                                                                                                                                >  1:847246/1‑150 (MQ=255)
      tGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCa                                                                                                                                              <  2:975923/150‑1 (MQ=255)
        cccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAgtcttgcg                                                                                                                                            <  1:883493/150‑8 (MQ=255)
        cccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                                            >  1:514533/1‑150 (MQ=255)
        cccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                                            <  1:784702/150‑1 (MQ=255)
         ccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCa                                                                                                                                              <  2:743685/147‑1 (MQ=255)
         ccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCa                                                                                                                                              >  1:743685/1‑147 (MQ=255)
           gCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGa                                                                                                                                         <  2:291979/150‑1 (MQ=255)
                ctgaGCACCGTCACGATGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGc                                                                                                                                    >  1:2199974/4‑150 (MQ=255)
                 ccAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGcc                                                                                                                                   <  2:430244/150‑1 (MQ=255)
                  cAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCa                                                                                                                                  >  2:158220/1‑150 (MQ=255)
                  cAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCa                                                                                                                                  >  1:2137080/1‑150 (MQ=255)
                       tCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACaa                                                                                                                             <  2:1921014/150‑1 (MQ=255)
                       tCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACaa                                                                                                                             <  2:514533/150‑1 (MQ=255)
                            aCGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACg                                                                                                                        <  1:1301218/150‑1 (MQ=255)
                                          tGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCaa                                                                                                          <  2:259182/150‑1 (MQ=255)
                                             cTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTc                                                                                                       >  1:707244/1‑150 (MQ=255)
                                             cTCGATCAAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTc                                                                                                       >  1:1532736/1‑150 (MQ=255)
                                              tCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTg                                                                                                                                           >  2:1903043/1‑113 (MQ=255)
                                              tCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTg                                                                                                                                           <  1:1903043/113‑1 (MQ=255)
                                              tCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCg                                                                                                      >  2:1903270/1‑150 (MQ=255)
                                              tCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCg                                                                                                      >  1:819497/1‑150 (MQ=255)
                                                gATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGAACAAGCTCAGCTCGgc                                                                                                    <  2:1799656/150‑1 (MQ=255)
                                                 aTCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGgcg                                                                                                   >  2:2171038/1‑150 (MQ=255)
                                                 aTCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGgcg                                                                                                   <  2:707244/150‑1 (MQ=255)
                                                     aGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATc                                                                                                                                      <  1:1908431/111‑1 (MQ=255)
                                                     aGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATc                                                                                                                                      >  2:1908431/1‑111 (MQ=255)
                                                       ttCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAg                                                                                            >  1:450734/1‑150 (MQ=255)
                                                          tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAggtg                                                                                         >  2:1786591/1‑150 (MQ=255)
                                                          tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAggtg                                                                                         <  1:1028671/150‑1 (MQ=255)
                                                              ccccAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACAAGGTGCGAGTGCATGGAAACGCAAGACTGTACGTTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCa                                                                                     >  1:1253232/1‑150 (MQ=255)
                                                               cccAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCAt                                                                                    >  1:1631436/1‑150 (MQ=255)
                                                                   accaaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGcc                                                                                <  2:1161793/150‑1 (MQ=255)
                                                                     caaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCa                                                                              <  2:497008/150‑1 (MQ=255)
                                                                     caaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCa                                                                              <  2:120588/150‑1 (MQ=255)
                                                                       aaGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGa                                                                            <  1:804714/150‑1 (MQ=255)
                                                                        tggggCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGAt                                                                           <  2:357306/149‑1 (MQ=255)
                                                                          gggCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGc                                                                         >  2:121110/1‑150 (MQ=255)
                                                                          gggCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGc                                                                         <  2:450734/150‑1 (MQ=255)
                                                                              ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGAc                                                                                                                         <  1:1958338/99‑1 (MQ=255)
                                                                              ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGAc                                                                                                                         >  2:1958338/1‑99 (MQ=255)
                                                                              ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGt                                                                     >  1:1905419/1‑150 (MQ=255)
                                                                              ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGt                                                                     >  1:2032630/1‑150 (MQ=255)
                                                                              ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGt                                                                     >  1:245055/1‑150 (MQ=255)
                                                                                  aGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTAcgc                                                                 <  2:1905419/150‑1 (MQ=255)
                                                                                     aaaaGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCggg                                                              >  1:1556759/1‑150 (MQ=255)
                                                                                     aaaaGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCggg                                                              <  2:2137080/150‑1 (MQ=255)
                                                                                       aaGCACCCACAGGACACGCTGCGAATGGACGGAACCCTTACACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATCACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGga                                                            <  1:738078/150‑1 (MQ=255)
                                                                                          cACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGgacga                                                         <  2:2199974/150‑1 (MQ=255)
                                                                                             ccACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAATGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACgg                                                      >  2:1664396/1‑150 (MQ=255)
                                                                                              cacaGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTc                                                                                                            >  1:2030377/1‑96 (MQ=255)
                                                                                              cacaGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTc                                                                                                            <  2:2030377/96‑1 (MQ=255)
                                                                                              cacaGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGc                                                     <  1:2171038/150‑1 (MQ=255)
                                                                                               acaGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGcc                                                    >  1:504428/1‑150 (MQ=255)
                                                                                                 aGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCccg                                                  <  2:1556759/150‑1 (MQ=255)
                                                                                                 aGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCccg                                                  >  1:336479/1‑150 (MQ=255)
                                                                                                    acacGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACg                                                                                                                        <  2:225852/78‑1 (MQ=255)
                                                                                                    acacGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACg                                                                                                                        >  1:225852/1‑78 (MQ=255)
                                                                                                         cTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTaaa                                          <  2:1631436/150‑1 (MQ=255)
                                                                                                            cGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGgt                                       <  1:121110/150‑1 (MQ=255)
                                                                                                              aaTGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGAAGACGGCCCGCCGGTAAAGttgt                                     <  2:761601/150‑1 (MQ=255)
                                                                                                               aTGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGtt                                    >  2:1750015/1‑150 (MQ=255)
                                                                                                                 ggACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGCCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGAGGGCCCGCCGGTAAAGGTGTTCt                                  >  1:1931790/1‑150 (MQ=255)
                                                                                                                  gACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCCCGACGGTGG‑CATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTg                                 <  2:1532736/150‑1 (MQ=255)
                                                                                                                  gACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTg                                 <  2:819497/150‑1 (MQ=255)
                                                                                                                  gACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTg                                 >  2:293767/1‑150 (MQ=255)
                                                                                                                   aCGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTgg                                <  2:2032630/150‑1 (MQ=255)
                                                                                                                        aaCGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACaa                           <  1:1786591/150‑1 (MQ=255)
                                                                                                                         aCGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATGGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAg                          <  2:2134450/150‑1 (MQ=255)
                                                                                                                         aCGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAg                          <  2:847246/150‑1 (MQ=255)
                                                                                                                          cGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAgg                         >  1:608282/1‑150 (MQ=255)
                                                                                                                          cGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAgg                         <  1:158220/150‑1 (MQ=255)
                                                                                                                          cGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGACAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAgg                         >  2:2009256/1‑150 (MQ=255)
                                                                                                                            cAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGacgacg                                                       >  2:1689879/1‑118 (MQ=255)
                                                                                                                            cAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGacgacg                                                       <  1:1689879/118‑1 (MQ=255)
                                                                                                                            cAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGc                       >  1:1412459/1‑150 (MQ=255)
                                                                                                                            cAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGc                       >  1:608813/1‑150 (MQ=255)
                                                                                                                             aaGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCa                      >  2:1409355/1‑150 (MQ=255)
                                                                                                                             aaGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCa                      >  1:524827/1‑150 (MQ=255)
                                                                                                                               tccTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCAc                                                                       <  1:1985722/97‑1 (MQ=255)
                                                                                                                               tccTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCAc                                                                       >  2:1985722/3‑99 (MQ=255)
                                                                                                                               gACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAAc                    >  1:858929/1‑150 (MQ=255)
                                                                                                                                 cTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAg                                         <  1:2303514/127‑1 (MQ=255)
                                                                                                                                 cTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAg                                         >  2:2303514/1‑127 (MQ=255)
                                                                                                                                 cTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACAt                  >  1:1546156/1‑150 (MQ=255)
                                                                                                                                   ggACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCa                <  1:1409355/150‑1 (MQ=255)
                                                                                                                                         tGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTgg          >  1:808602/1‑150 (MQ=255)
                                                                                                                                         tGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTgg          <  2:524827/150‑1 (MQ=255)
                                                                                                                                          ggCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTggg         <  2:808602/150‑1 (MQ=255)
                                                                                                                                           gCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTgggg        <  2:1253232/150‑1 (MQ=255)
                                                                                                                                                 gcCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTGGGGTTTCgg  <  1:2009256/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCG‑CAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTGGGGTTTCGG  >  NC_002947/4300969‑4301263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑