Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,479,857 A→G 5.2% P388P (CCA→CCG)  ybdR → Zn‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,479,8570AG 5.2% 301.7 / 4.1 97P388P (CCA→CCG) ybdRZn‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (43/48);  new base G (3/2);  total (47/50)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

ACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGA  >  NC_002947/4479709‑4480006
                                                                                                                                                    |                                                                                                                                                     
aCCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAg                                                                                                                                                      <  1:306175/150‑1 (MQ=255)
  cTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGt                                                                                                                                                    >  2:2138358/1‑150 (MQ=255)
   ttCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGtg                                                                                                                                                   <  2:271435/150‑1 (MQ=255)
    tCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGtgc                                                                                                                                                  >  2:334549/1‑150 (MQ=255)
     cccGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGtgct                                                                                                                                                 <  2:947504/150‑1 (MQ=255)
     cccGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGACAGGtgct                                                                                                                                                 <  1:1038489/150‑1 (MQ=255)
       cGAACTGCTGGAGCACATCGCAGCAGGGCGCCTGCAACCCGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCACGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGtgctgc                                                                                                                                               <  2:1026370/150‑1 (MQ=255)
       cGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGtgctgc                                                                                                                                               >  2:923791/1‑150 (MQ=255)
              ctgGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGc                                                                                                                                        <  2:2102038/150‑1 (MQ=255)
                ggAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCAc                                                                                                                                      <  2:254644/150‑1 (MQ=255)
                 gAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCAcc                                                                                                                                     >  2:1588376/1‑150 (MQ=255)
                    cacaTCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTg                                                                                                                                  <  1:274843/150‑1 (MQ=255)
                       aTCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAg                                                                                                                                                      >  1:570081/1‑127 (MQ=255)
                       aTCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAg                                                                                                                                                      <  2:570081/127‑1 (MQ=255)
                       aTCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGc                                                                                                                               >  2:145442/1‑150 (MQ=255)
                        tCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGcc                                                                                                                              >  1:1680231/1‑150 (MQ=255)
                          gAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCt                                                                                                                            >  2:1528824/1‑150 (MQ=255)
                              cAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGacc                                                                                                                        <  2:1935174/150‑1 (MQ=255)
                                       tGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTaga                                                                                                               <  1:1989225/150‑1 (MQ=255)
                                          aaCCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAgagga                                                                                                            <  1:391123/150‑1 (MQ=255)
                                          aaCCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAgagga                                                                                                            >  1:1533796/1‑150 (MQ=255)
                                             cGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCGGGTGCAGCAc                                                                                                                                             >  2:2398364/1‑114 (MQ=255)
                                             cGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCGGGTGCAGCAc                                                                                                                                             <  1:2398364/114‑1 (MQ=255)
                                              ggAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTaga                                                                                                               <  2:544293/143‑1 (MQ=255)
                                              ggAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTaga                                                                                                               >  1:544293/1‑143 (MQ=255)
                                              ggAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGaa                                                                                                        >  1:1678071/1‑150 (MQ=255)
                                              ggAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGaa                                                                                                        <  1:334549/150‑1 (MQ=255)
                                              ggAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGaa                                                                                                        <  1:1588376/150‑1 (MQ=255)
                                                  cTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGAACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGAt                                                                                                    <  2:857499/150‑1 (MQ=255)
                                                   tGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTTCCAGGTGCTGCACCCTGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATc                                                                                                   >  1:398324/1‑150 (MQ=255)
                                                    gATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCa                                                                                                  >  2:623267/1‑150 (MQ=255)
                                                        gTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCGGGTGCAGCACCTGGCAcga                                                                                                                                    >  1:2124894/1‑110 (MQ=255)
                                                        gTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCGGGTGCAGCACCTGGCAcga                                                                                                                                    <  2:2124894/112‑3 (MQ=255)
                                                         ttACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAAc                                                                                             <  2:364380/150‑1 (MQ=255)
                                                          tACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACg                                                                                            <  1:145442/150‑1 (MQ=255)
                                                             tCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACct                                                                                                                    <  2:710864/123‑1 (MQ=255)
                                                             tCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACct                                                                                                                    >  1:710864/1‑123 (MQ=255)
                                                               aCCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGtctc                                                                                       <  2:1680231/150‑1 (MQ=255)
                                                                       gCCCGAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGCTa                                                                               <  1:2401613/150‑1 (MQ=255)
                                                                        cccTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTagag                                                                                                              >  2:2232641/1‑118 (MQ=255)
                                                                        cccTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTagag                                                                                                              <  1:2232641/118‑1 (MQ=255)
                                                                         ccTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCGGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACt                                                                             >  2:1270323/1‑150 (MQ=255)
                                                                          cTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTg                                                                                                                                  <  1:449168/96‑1 (MQ=255)
                                                                          cTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTg                                                                                                                                  >  2:449168/1‑96 (MQ=255)
                                                                          cTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACtt                                                                            >  2:1691481/1‑150 (MQ=255)
                                                                                 agGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGAtt                                                                     >  2:2396799/1‑150 (MQ=255)
                                                                                        cATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAg                                                              <  1:177882/150‑1 (MQ=255)
                                                                                         aTGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGc                                                             >  2:2162753/1‑150 (MQ=255)
                                                                                              cTAAAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCATCCGGCACCTTGGGCCTTCACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCaa                                                        <  2:77832/150‑1 (MQ=255)
                                                                                               tACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGaa                                                                                              >  1:982583/1‑111 (MQ=255)
                                                                                               tACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGaa                                                                                              <  2:982583/111‑1 (MQ=255)
                                                                                               tACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAAt                                                       >  2:1984012/1‑150 (MQ=255)
                                                                                                    aaTGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAAttcttc                                                  <  1:1691481/150‑1 (MQ=255)
                                                                                                      tGTTCGATCAAAAGCAGGATAACTGCCGCAAGACAATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTg                                                >  1:1397326/1‑150 (MQ=255)
                                                                                                       gTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGc                                               <  1:623267/150‑1 (MQ=255)
                                                                                                            aTCAAACGCAGGATAACTGCCGCAAGGGCATCCCAGTCCCCGGTGCTGCACCCGGCACCTTGAGCCCTAAGCGCCTCTCGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTGCTGGATTGCCGTAGCGGCAACTCTTCTGCTCATc                                          >  2:233387/1‑150 (MQ=255)
                                                                                                            aTCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATc                                          >  2:2277261/1‑150 (MQ=255)
                                                                                                             tCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCg                                         >  1:1515477/1‑150 (MQ=255)
                                                                                                              cAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGa                                        <  2:1533796/150‑1 (MQ=255)
                                                                                                               aaaaGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGAc                                       <  1:2162753/150‑1 (MQ=255)
                                                                                                                aaaGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATGGCCGTAGCGGCAATTCTTCTGCTCATCGAcc                                      <  1:923791/150‑1 (MQ=255)
                                                                                                                 aaGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAAttctt                                                   >  2:2042493/1‑136 (MQ=255)
                                                                                                                 aaGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAAttctt                                                   <  1:2042493/136‑1 (MQ=255)
                                                                                                                    cAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTCACGGTAATTCTTCTGCTCATCGACCtgtg                                  <  1:1270323/150‑1 (MQ=255)
                                                                                                                     aGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTgg                                                                                                                                 >  1:1999605/1‑54 (MQ=255)
                                                                                                                     aGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTgg                                                                                                                                 <  2:1999605/54‑1 (MQ=255)
                                                                                                                      ggATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTggg                                >  1:2228239/1‑150 (MQ=255)
                                                                                                                        aTAACTGCCGCAAGGTGATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGCTCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGTGcc                              <  2:741024/150‑1 (MQ=255)
                                                                                                                         tAACTGCAGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCTTTAAGTCTTCTGCTCATGGATCTGTGGGCCa                             <  2:398324/150‑1 (MQ=255)
                                                                                                                           aCTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATc                           <  2:1678071/150‑1 (MQ=255)
                                                                                                                           aCTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATc                           <  1:180550/150‑1 (MQ=255)
                                                                                                                           aCTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATc                           >  2:180550/1‑150 (MQ=255)
                                                                                                                           aCTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATc                           >  2:80016/1‑150 (MQ=255)
                                                                                                                           aCTGCCGCAAGGTCATCCTCGTGCCAGGTGCTCCACTCTGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCCTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATc                           <  2:1397326/150‑1 (MQ=255)
                                                                                                                            cTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCtgtg                                  >  1:113052/1‑142 (MQ=255)
                                                                                                                            cTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCtgtg                                  <  2:113052/142‑1 (MQ=255)
                                                                                                                             tGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGtctc                                                                                       <  2:818735/88‑1 (MQ=255)
                                                                                                                             tGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGtctc                                                                                       >  1:818735/1‑88 (MQ=255)
                                                                                                                               ccGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCa                       >  2:1959213/1‑150 (MQ=255)
                                                                                                                                 gCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGc                     <  2:2228239/150‑1 (MQ=255)
                                                                                                                                 gCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGc                     <  1:1959213/150‑1 (MQ=255)
                                                                                                                                   aaGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCgt                   >  2:988981/1‑150 (MQ=255)
                                                                                                                                    aGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCgtg                  <  1:1984012/150‑1 (MQ=255)
                                                                                                                                     ggTCATCCTCGCGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGCACTGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCCACCTGTGGGCCATCGTCAGCgtgt                 >  2:817896/1‑150 (MQ=255)
                                                                                                                                      gTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGtt                >  1:1927971/1‑150 (MQ=255)
                                                                                                                                         aTCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGc                                                                   >  2:240073/1‑96 (MQ=255)
                                                                                                                                         aTCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGc                                                                   <  1:240073/96‑1 (MQ=255)
                                                                                                                                         aTCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATc                                          <  1:1219527/121‑1 (MQ=255)
                                                                                                                                         aTCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATc                                          >  2:1219527/1‑121 (MQ=255)
                                                                                                                                          tCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCgg            >  2:1019947/1‑150 (MQ=255)
                                                                                                                                          ccaaggTGCCGGGTGCAGCACCTGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTAATCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCgg            >  1:23047/6‑150 (MQ=255)
                                                                                                                                            cTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAg          >  1:837115/1‑150 (MQ=255)
                                                                                                                                             tCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCt                                                                                                                            <  1:2183190/35‑1 (MQ=255)
                                                                                                                                             tCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCt                                                                                                                            >  2:2183190/1‑35 (MQ=255)
                                                                                                                                             tCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGc         <  1:2277261/150‑1 (MQ=255)
                                                                                                                                             tCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGc         >  1:140918/1‑150 (MQ=255)
                                                                                                                                                 gCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACa     >  1:993077/1‑150 (MQ=255)
                                                                                                                                                    aGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGa  <  1:1528824/150‑1 (MQ=255)
                                                                                                                                                    aGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGa  >  2:1860520/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGA  >  NC_002947/4479709‑4480006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑