Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,490,333 C→A 8.0% Q525H (CAG→CAT) ‡ PP_3982 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,490,3330CA 8.0% 276.8 / 10.1 87Q525H (CAG→CAT) ‡PP_3982hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (39/41);  new base A (3/4);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

CTTCGCAACAAGGCCAAAAACTCGGCATAAGCCGCCTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGAACCAGCCCAATCCACCGC  >  NC_002947/4490184‑4490482
                                                                                                                                                     |                                                                                                                                                     
cTTCGCAACAAGGCCAAAAACTCGGCATAAGCCGCCTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCAcc                                                                                                                                                       >  2:1292052/1‑150 (MQ=255)
 ttCGCAACAAGGCCAAAAACTCGGCATAAGCCGCCTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCAccc                                                                                                                                                      <  2:420569/150‑2 (MQ=255)
 ttCGCAACAAGGCCAAAAACTCGGCATAAGCCGCCTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCt                                                                                                                                                      >  1:560611/1‑150 (MQ=255)
        caaGGCCAAAAACTCGGCATAAGCCGCCTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGtgcttg                                                                                                                                               >  2:61412/1‑150 (MQ=255)
         aaGGCCAAAAACTCGGCATAAGCCGCCTGGATTCGCCCCGCAGCGGCAATCAGGTCGCGATCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACAGGtgcttgt                                                                                                                                              <  2:1770255/150‑2 (MQ=255)
         aaGGCCAAAAACTCGGCATAAGCCGCCTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGtgcttgc                                                                                                                                              >  2:1924216/1‑150 (MQ=255)
         aaGGCCAAAAACTCGGCATAAGCCGCCTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACAGGtgcttgt                                                                                                                                              <  2:1273553/150‑2 (MQ=255)
               aaaaaCTCGGCATAAGCCGCCTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGcctc                                                                                                                                        <  1:743204/150‑1 (MQ=255)
               aaaaaCTCGGCATAAGCCGCCTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGcctc                                                                                                                                        <  1:1544115/150‑1 (MQ=255)
                               ccgccTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTg                                                                                                                        >  2:1163985/1‑150 (MQ=255)
                               ccgccTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTg                                                                                                                        >  1:2042875/1‑150 (MQ=255)
                                     ggATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGCACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTGGCTGCCTCCTGGCCGCATGCTCTGCCAGTg                                                                                                                  >  1:2340056/1‑150 (MQ=255)
                                        tgcGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTa                                                                                                               >  1:1985950/3‑150 (MQ=255)
                                         tCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTAt                                                                                                              <  1:1924216/150‑1 (MQ=255)
                                          cGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTAtt                                                                                                             <  2:2042875/150‑1 (MQ=255)
                                           gCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGtgcttgct                                                                                                                                             <  2:2128654/117‑1 (MQ=255)
                                           gCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGtgcttgct                                                                                                                                             >  1:2128654/1‑117 (MQ=255)
                                           gCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGtgcttg                                                                                                                                               <  2:1075694/115‑1 (MQ=255)
                                           gCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGtgcttg                                                                                                                                               >  1:1075694/1‑115 (MQ=255)
                                           gCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGAGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTc                                                                                                            <  2:2401004/150‑1 (MQ=255)
                                             aCCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTg                                                                                                                  <  2:807309/142‑1 (MQ=255)
                                             aCCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTg                                                                                                                  >  1:807309/1‑142 (MQ=255)
                                               cTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCAATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTCGCCACATGCTCTGCCAGTGCTATTCAAgc                                                                                                        >  2:141584/1‑150 (MQ=255)
                                                tCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAgc                                                                                                        >  2:2290527/1‑149 (MQ=255)
                                                tCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAgc                                                                                                        <  1:2290527/149‑1 (MQ=255)
                                                     ggCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGtt                                                                                                  >  1:358196/1‑150 (MQ=255)
                                                         aTCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGtgcttg                                                                                                                                               >  1:1223491/1‑101 (MQ=255)
                                                         aTCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGtgcttg                                                                                                                                               <  2:1223491/101‑1 (MQ=255)
                                                         aTCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGa                                                                                              <  1:2042479/150‑1 (MQ=255)
                                                            aGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGa                                                                                           <  1:1292052/150‑1 (MQ=255)
                                                            aGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGa                                                                                           <  2:1452723/150‑1 (MQ=255)
                                                              gTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGAc                                                                                             >  2:546779/1‑146 (MQ=255)
                                                              gTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGAc                                                                                             <  1:546779/146‑1 (MQ=255)
                                                                 gcgcTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGAt                                                                                          >  1:2402251/1‑146 (MQ=255)
                                                                 gcgcTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGAt                                                                                          <  2:2402251/146‑1 (MQ=255)
                                                                    ctctACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTc                                                                                   <  1:1516745/150‑1 (MQ=255)
                                                                       tACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGa                                                                                                          >  2:311630/1‑124 (MQ=255)
                                                                       tACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGa                                                                                                          <  1:311630/124‑1 (MQ=255)
                                                                       tACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGAtctc                                                                                       >  1:690056/1‑143 (MQ=255)
                                                                       tACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGAtctc                                                                                       <  2:690056/143‑1 (MQ=255)
                                                                       tACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGc                                                                                >  1:554034/1‑150 (MQ=255)
                                                                       tACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGc                                                                                <  1:476036/150‑1 (MQ=255)
                                                                          agcagcCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCGTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCACTGCTATTCGAGCGCTGCTCCGACGATCTCGTTCAGCGAc                                                                             >  1:735328/1‑150 (MQ=255)
                                                                           gcagcCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGAc                                                                                             <  2:1235838/133‑1 (MQ=255)
                                                                           gcagcCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGAc                                                                                             >  1:1235838/1‑133 (MQ=255)
                                                                               ccGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAggg                                                                        <  2:554034/150‑1 (MQ=255)
                                                                                   tGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGtgctt                                                                                                                                                >  1:1785685/1‑74 (MQ=255)
                                                                                   tGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGtgctt                                                                                                                                                <  2:1785685/74‑1 (MQ=255)
                                                                                    gCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGc                                                                   <  2:829529/150‑1 (MQ=255)
                                                                                        tCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTAt                                                               >  2:1091494/1‑150 (MQ=255)
                                                                                        tCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGCGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTAt                                                               <  1:1803473/150‑1 (MQ=255)
                                                                                            cacaGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGc                                                           <  2:1985950/150‑1 (MQ=255)
                                                                                                      tGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGAATTCTGGCCGCATGCTCTGCAAGTGGTATTCGCGCGATGTTCACAACGTCTAGTTGAGAGAGCAGGGTCTGCCTAAGTTCAAGACTctgc                                                 >  2:758669/1‑150 (MQ=255)
                                                                                                         aCCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGa                                              <  2:358196/150‑1 (MQ=255)
                                                                                                         aCCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGa                                              <  2:560611/150‑1 (MQ=255)
                                                                                                           cTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGAtct                                                                                        >  1:1249404/1‑106 (MQ=255)
                                                                                                           cTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGAtct                                                                                        <  2:1249404/106‑1 (MQ=255)
                                                                                                           cTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGt                                            >  1:1157692/1‑150 (MQ=255)
                                                                                                            tCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGGTCTCGTTCAGCGACTAGGGTCGGCCTATGTGCAAGACTCTGCTGAGtg                                           >  1:2322895/1‑150 (MQ=255)
                                                                                                                 cTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGAc                                      >  1:1722955/1‑150 (MQ=255)
                                                                                                                  tCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTATTGTCTGCCTATGTGCAAGACTCTGCTGAGt                                            >  1:1330455/1‑143 (MQ=255)
                                                                                                                  tCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGt                                            <  2:1330455/143‑1 (MQ=255)
                                                                                                                    aCCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGc                                                                                                                                   <  1:798183/54‑1 (MQ=255)
                                                                                                                    aCCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGc                                                                                                                                   >  2:798183/1‑54 (MQ=255)
                                                                                                                    aCCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGa                                                                                                          >  2:1075316/1‑79 (MQ=255)
                                                                                                                    aCCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGa                                                                                                          <  1:1075316/79‑1 (MQ=255)
                                                                                                                    aCCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTAtgt                                                             <  2:1789946/124‑1 (MQ=255)
                                                                                                                    aCCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTAtgt                                                             >  1:1789946/1‑124 (MQ=255)
                                                                                                                      cAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCa                                                                                  <  1:1965299/101‑1 (MQ=255)
                                                                                                                      cAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCa                                                                                  >  2:1965299/1‑101 (MQ=255)
                                                                                                                       aaTGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTGCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGCTCTCGTCCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACAtcttc                                <  2:147034/150‑1 (MQ=255)
                                                                                                                        aTGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTCGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTGCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACAtcttct                               <  1:758669/150‑1 (MQ=255)
                                                                                                                             ttGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTc                          >  1:973227/1‑150 (MQ=255)
                                                                                                                                  ttGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGa                                              <  1:1197072/125‑1 (MQ=255)
                                                                                                                                  ttGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGa                                              >  2:1197072/1‑125 (MQ=255)
                                                                                                                                   tGCCGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGGCGATCTCGTTCTGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGa                    <  2:1073660/150‑1 (MQ=255)
                                                                                                                                       gCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACAt                                    >  2:964751/1‑130 (MQ=255)
                                                                                                                                       gCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACAt                                    <  1:964751/130‑1 (MQ=255)
                                                                                                                                          tCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGAACCAGcc             <  2:973227/150‑1 (MQ=255)
                                                                                                                                            gACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGc                                                                                                                       <  1:2377589/42‑1 (MQ=255)
                                                                                                                                            gACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGc                                                                                                                       >  2:2377589/1‑42 (MQ=255)
                                                                                                                                            agcAAGCACAGGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATcttctt                              >  2:1429067/3‑131 (MQ=255)
                                                                                                                                            agcAAGCACAGGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATcttctt                              <  1:1429067/129‑1 (MQ=255)
                                                                                                                                            agcAAGCACAGGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGaa                   <  1:2244172/140‑1 (MQ=255)
                                                                                                                                            agcAAGCACAGGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGaa                   >  2:2244172/3‑142 (MQ=255)
                                                                                                                                             gcAAGCACAGGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGAACCAGCCCaa          >  2:2164063/2‑150 (MQ=255)
                                                                                                                                             aCAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGAACCAGCCCaa          >  2:1426994/1‑150 (MQ=255)
                                                                                                                                             aCAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGAACCAGCCCaa          >  2:108000/1‑150 (MQ=255)
                                                                                                                                                  cACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGAACCAGCCCAATCCAc     <  1:1404525/150‑1 (MQ=255)
                                                                                                                                                     cTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAgg                                                                         <  2:2223006/79‑1 (MQ=255)
                                                                                                                                                     cTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGAACCAGCCCAATCCACCGc  >  1:890798/1‑150 (MQ=255)
                                                                                                                                                     cTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGAACCAGCCCAATCCACCGc  >  1:1838111/1‑150 (MQ=255)
                                                                                                                                                     cTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGAACCAGCCCAATCCACCGc  <  2:1838111/150‑1 (MQ=255)
                                                                                                                                                     cTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGAATAgg                                                                         >  1:2223006/1‑79 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTCGCAACAAGGCCAAAAACTCGGCATAAGCCGCCTGGATTCGCACCTCAGCGGCAATCAGGTCGCGCTCTACAGCAGCCGCTGCGATCAGCACAGCGTTCTGCACCTCGAACTCACCAATGCGTTGTCTTGCAGCGTCGACAAGCACCTGTGCTTGCTGCCTCCTGGCCGCATGCTCTGCCAGTGCTATTCGAGCGCTGTTCAGACGATCTCGTTCAGCGACTAGGGTCTGCCTATGTGCAAGACTCTGCTGAGTGATGACATCTTCTTGCTCCAATGAACCAGCCCAATCCACCGC  >  NC_002947/4490184‑4490482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑