Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,512,509 T→G 10.2% N8T (AAC→ACC)  lolA ← outer membrane lipoprotein carrier protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,512,5090TG10.2% 274.9 / 15.7 102N8T (AAC→ACC) lolAouter membrane lipoprotein carrier protein
Reads supporting (aligned to +/- strand):  ref base T (46/45);  new base G (6/5);  total (52/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GCGACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAGCTTGC  >  NC_002947/4512360‑4512658
                                                                                                                                                     |                                                                                                                                                     
gCGACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGt                                                                                                                                                       >  2:1954147/1‑150 (MQ=255)
  gACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTg                                                                                                                                                     >  2:115805/1‑150 (MQ=255)
        tCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCTCCAACGGTGGCGCca                                                                                                                                               <  2:2153639/150‑1 (MQ=255)
        tCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCca                                                                                                                                               <  2:1905506/150‑1 (MQ=255)
        tCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCca                                                                                                                                               <  1:1763812/150‑1 (MQ=255)
         ccAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcag                                                                                                                                              <  1:2054524/150‑1 (MQ=255)
         ccAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcag                                                                                                                                              <  2:1604804/150‑1 (MQ=255)
          cAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcagg                                                                                                                                             <  1:601678/150‑1 (MQ=255)
           aGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcaggc                                                                                                                                            >  2:1638824/1‑150 (MQ=255)
           aGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcag                                                                                                                                              >  1:1819523/1‑148 (MQ=255)
           aGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcag                                                                                                                                              <  2:1819523/148‑1 (MQ=255)
            gCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcaggcc                                                                                                                                           >  1:1407550/1‑149 (MQ=255)
              aaCTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcaggcagg                                                                                                                                         <  1:229655/150‑1 (MQ=255)
              aaCTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcaggcctt                                                                                                                                         <  2:1407550/150‑4 (MQ=255)
               aCTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCca                                                                                                                                               <  2:1302470/143‑1 (MQ=255)
               aCTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCca                                                                                                                                               >  1:1302470/1‑143 (MQ=255)
                      cAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTtcat                                                                                                                                 <  1:1597709/150‑1 (MQ=255)
                        ggCGTTGTACGTCTTGCTCCCCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTtcatca                                                                                                                               <  2:1587232/150‑1 (MQ=255)
                               tACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTcgcgc                                                                                                                        >  2:836115/1‑150 (MQ=255)
                                  gTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCggg                                                                                                                     >  2:1413939/1‑150 (MQ=255)
                                    cTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGgcc                                                                                                                   >  2:308097/1‑150 (MQ=255)
                                         tCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGTGAATCCTACTTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCg                                                                                                              >  1:654180/1‑150 (MQ=255)
                                         tCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCg                                                                                                              >  1:1617730/1‑150 (MQ=255)
                                          cACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGAGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCgg                                                                                                             <  2:2259763/150‑1 (MQ=255)
                                           aacGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCggg                                                                                                            >  2:91172/3‑150 (MQ=255)
                                           aCCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCggg                                                                                                            <  2:2123036/150‑1 (MQ=255)
                                            ccGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCgggg                                                                                                           <  1:115805/150‑1 (MQ=255)
                                            ccGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCgggg                                                                                                           <  1:1121366/150‑1 (MQ=255)
                                               gCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTa                                                                                                        >  2:2065556/1‑150 (MQ=255)
                                                 aTAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATc                                                                                                      >  2:490984/1‑150 (MQ=255)
                                                      cAGTAACGGAGCCCAGGGTCAGGGCAGAAACCAACAGAATGCAAATCGCGCGCATGGGAATCTTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGACCGCCCGGGGCTATCACTTc                                                                                                 >  2:1892058/1‑150 (MQ=255)
                                                           aCCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTtcat                                                                                                                                 <  2:169913/113‑1 (MQ=255)
                                                           aCCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTtcat                                                                                                                                 >  1:169913/1‑113 (MQ=255)
                                                                 cccAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGt                                                                                      <  1:490984/150‑1 (MQ=255)
                                                                 cccAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCACGCAAGACGt                                                                                      >  1:2213133/1‑150 (MQ=255)
                                                                  ccAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGtt                                                                                     >  2:316163/1‑150 (MQ=255)
                                                                  ccAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGtt                                                                                     >  2:1068413/1‑150 (MQ=255)
                                                                  ccAGCGTCAGGGCAGAAACCAACGGCCTGCGAATCGCGCGCACGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCTCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGtt                                                                                     <  1:1624628/150‑1 (MQ=255)
                                                                   cAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCCCTTCCCGCGAGCCGTTg                                                                                    >  1:529042/1‑150 (MQ=255)
                                                                      ggTCAGGGCAGAAACCAACCGCATGCGAATCGAGCGCATGGGAATCCTTATTTGGCGTGTAAAAGGCCTTGCGCCACCGTTGGCGCCAGGCAGGGTGTTCCCCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTg                                                                                 <  2:529042/150‑1 (MQ=255)
                                                                       gTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCACTGCCAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGTCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGt                                                                                <  1:1954147/150‑1 (MQ=255)
                                                                           gggCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCAt                                                                            >  1:2115534/1‑150 (MQ=255)
                                                                              cAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTAGAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCgg                                                                         >  1:2351856/1‑150 (MQ=255)
                                                                                 aaaCCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGt                                                                      <  1:836115/150‑1 (MQ=255)
                                                                                   aCCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGa                                                                    >  2:1794018/1‑150 (MQ=255)
                                                                                     cAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcag                                                                                                                                              <  2:2348503/74‑1 (MQ=38)
                                                                                     cAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcag                                                                                                                                              >  1:2348503/1‑74 (MQ=255)
                                                                                       aCAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCTCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAAc                                                                <  2:1158433/150‑1 (MQ=255)
                                                                                           cATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCg                                                            <  1:316163/150‑1 (MQ=255)
                                                                                           cATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCg                                                            <  1:91172/150‑1 (MQ=255)
                                                                                            aTGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTcgcgc                                                                                                                        <  1:2276058/89‑1 (MQ=255)
                                                                                            aTGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTcgcgc                                                                                                                        >  2:2276058/1‑89 (MQ=255)
                                                                                            aTGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCgg                                                           <  1:2065556/150‑1 (MQ=255)
                                                                                                  aTCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGgtg                                                                                                                                      >  2:2096066/1‑69 (MQ=255)
                                                                                                  aTCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGgtg                                                                                                                                      <  1:2096066/69‑1 (MQ=255)
                                                                                                  aTCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcag                                                                                                                                              <  2:1870319/61‑1 (MQ=37)
                                                                                                  aTCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcag                                                                                                                                              >  1:1870319/1‑61 (MQ=37)
                                                                                                   tCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGa                                                                                           >  2:1348729/1‑111 (MQ=255)
                                                                                                   tCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGa                                                                                           <  1:1348729/111‑1 (MQ=255)
                                                                                                    cgcgcgCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTcc                                                   <  2:2115534/150‑1 (MQ=255)
                                                                                                      cgcgcATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCAt                                                 <  1:1638824/150‑1 (MQ=255)
                                                                                                       gcgcATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATc                                                >  2:2273623/1‑150 (MQ=255)
                                                                                                         gcATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGa                                              >  1:10944/1‑150 (MQ=255)
                                                                                                          cATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGAc                                             <  2:2033240/150‑1 (MQ=255)
                                                                                                          cATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGAc                                             <  2:426737/150‑1 (MQ=255)
                                                                                                           aTGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGa                                                                    <  1:1607055/126‑1 (MQ=255)
                                                                                                           aTGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGa                                                                    >  2:1607055/1‑126 (MQ=255)
                                                                                                             gggAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCg                                          >  2:431446/1‑150 (MQ=255)
                                                                                                                aaTCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCg                                                            <  1:1868409/129‑1 (MQ=255)
                                                                                                                aaTCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCg                                                            >  2:1868409/1‑129 (MQ=255)
                                                                                                                  tCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCAt                                     <  1:1794018/150‑1 (MQ=255)
                                                                                                                   ccTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGCAATTGACTTTCCGCGAGCCGTTGCTGTTCATCGGGGCGACAACGCCGGCCATCTCCATCGACTCGATCATg                                    >  1:1621344/1‑150 (MQ=255)
                                                                                                                       aTTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgcgcg                                <  2:1196054/150‑1 (MQ=255)
                                                                                                                       aTTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgcgcg                                >  1:1196054/1‑150 (MQ=255)
                                                                                                                         tGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGcgcgcg                              <  2:654180/150‑1 (MQ=255)
                                                                                                                         tGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGcgcgcg                              <  1:431446/150‑1 (MQ=255)
                                                                                                                            gCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCa                           >  2:2348813/1‑150 (MQ=255)
                                                                                                                               ttGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACgg                        >  1:158300/1‑150 (MQ=255)
                                                                                                                               ttGAAAAGGCCTGCCGCCACCGTTCCCACCAGGCAGGGTTTTCAGCAGTCGCGCGGACCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGcgccg                        <  1:1892058/150‑5 (MQ=255)
                                                                                                                                tgtacaGGCCGGGCGCCACCGTTGTGGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGt                       <  2:390423/145‑1 (MQ=255)
                                                                                                                                 gAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGtt                      <  1:308097/150‑1 (MQ=255)
                                                                                                                                 gAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGtt                      >  1:2111109/1‑150 (MQ=255)
                                                                                                                                  aaaaGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGa                                         <  2:2228778/130‑1 (MQ=255)
                                                                                                                                  aaaaGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGa                                         >  1:2228778/1‑130 (MQ=255)
                                                                                                                                  aaaaGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgc                                   <  2:218730/136‑1 (MQ=255)
                                                                                                                                  aaaaGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgc                                   >  1:218730/1‑136 (MQ=255)
                                                                                                                                    aaGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACACCGcc                                                             <  2:645679/108‑1 (MQ=255)
                                                                                                                                    aaGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGcc                                                             >  1:645679/1‑108 (MQ=255)
                                                                                                                                    aaGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGttaga                   >  1:390423/1‑147 (MQ=255)
                                                                                                                                         cTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGa              <  2:1617730/150‑1 (MQ=255)
                                                                                                                                         cTGGCGCCAACGGTGGCGCCAGGCCGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGa              >  1:1257168/1‑150 (MQ=255)
                                                                                                                                         cTGGCGCCAACGGTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGGGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACCGTTGTAGCCGa              >  1:2102359/1‑150 (MQ=255)
                                                                                                                                         cTGGCGCCAACGGTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGa              >  2:367658/1‑150 (MQ=255)
                                                                                                                                         cTGGCGCCAACGGTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGa              <  1:367658/150‑1 (MQ=255)
                                                                                                                                          tGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGGTCGGCACGGTTGTAGCCGAt             >  2:1373114/1‑150 (MQ=255)
                                                                                                                                          tGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGAt             >  2:1754496/1‑150 (MQ=255)
                                                                                                                                                cACCGTTGGCGCCAGGCAGGTTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGGACGGTTGTAGCCGATCTTCAg       >  2:1241628/1‑150 (MQ=255)
                                                                                                                                                 aCCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAGc      >  1:1061550/1‑150 (MQ=255)
                                                                                                                                                    gTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAGCTTg   >  2:1073463/1‑150 (MQ=255)
                                                                                                                                                     ttGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAGCTTGc  >  1:1591409/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAGCTTGC  >  NC_002947/4512360‑4512658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑