Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 358,099 C→T 10.9% intergenic (+64/‑66) tdcG‑I → / → PP_5441 L‑serine dehydratase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947358,0990CT10.9% 121.6 / 6.1 46intergenic (+64/‑66)tdcG‑I/PP_5441L‑serine dehydratase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/21);  new base T (2/3);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

CATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGG  >  NC_002947/357951‑358240
                                                                                                                                                    |                                                                                                                                             
cATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTGCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATcc                                                                                                                                              <  2:177866/150‑1 (MQ=255)
   gcgcGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTg                                                                                                                                           <  1:1014904/150‑1 (MQ=255)
   gcgcGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGAGCAATCCCTg                                                                                                                                           <  2:607349/150‑1 (MQ=255)
     gcgATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTa                                                                                                                                         <  1:98193/150‑1 (MQ=255)
      cgATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCACGCGAAGCGGACTGACGGTTAGCGTTATCGTGTGCTGATTTTCTGATCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAg                                                                                                                                        <  2:1476506/150‑1 (MQ=255)
       gATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAg                                                                                                                                        >  2:153591/1‑149 (MQ=255)
       gATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAg                                                                                                                                        <  1:153591/149‑1 (MQ=255)
             ggCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCggg                                                                                                                                 <  1:2387121/150‑1 (MQ=25)
             ggCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCggg                                                                                                                                 <  1:560558/150‑1 (MQ=25)
                    atatGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCAGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGccc                                                                                                                          <  1:874953/150‑1 (MQ=21)
                      atGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAACCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCcgg                                                                                                                        <  2:1652797/150‑2 (MQ=255)
                        gCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCt                                                                                                                                            <  2:2113711/128‑1 (MQ=255)
                        gCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCt                                                                                                                                            >  1:2113711/1‑128 (MQ=255)
                          aCGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATccc                                                                                                                                             <  2:511904/125‑1 (MQ=255)
                          aCGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATccc                                                                                                                                             >  1:511904/1‑125 (MQ=255)
                          aCGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAag                                                                                                                    >  2:595312/1‑150 (MQ=255)
                                       agagACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGaa                                                                                                                     >  2:2404845/1‑136 (MQ=255)
                                       agagACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCCaa                                                                                                                     <  1:2404845/136‑1 (MQ=255)
                                         agaCCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTg                                                                                                     <  1:595312/150‑1 (MQ=255)
                                                           tGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTa                                                                                                                                         >  1:1034845/1‑96 (MQ=255)
                                                           tGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTa                                                                                                                                         <  2:1034845/96‑1 (MQ=255)
                                                                                             gCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGgcgc                                                 >  1:284120/1‑150 (MQ=255)
                                                                                               tGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGccc                                                                                                              >  1:1663172/1‑87 (MQ=255)
                                                                                               tGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGccc                                                                                                              <  2:1663172/87‑1 (MQ=255)
                                                                                                 aCCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCg                                             >  1:1779549/1‑150 (MQ=255)
                                                                                                     tCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTa                                         <  2:1779549/150‑1 (MQ=255)
                                                                                                            ctctTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGAtcg                                  >  1:1968387/1‑150 (MQ=255)
                                                                                                               ttCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGc                                                                                                                                    <  1:2024836/49‑1 (MQ=255)
                                                                                                               ttCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGc                                                                                                                                    >  2:2024836/1‑49 (MQ=255)
                                                                                                                    gggCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGGGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGt                          >  1:1907777/1‑150 (MQ=255)
                                                                                                                    gggCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGt                          >  2:1338911/1‑150 (MQ=255)
                                                                                                                          gCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGAt                    >  1:350643/1‑150 (MQ=25)
                                                                                                                          gCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGAt                                                                                                   <  1:2174430/71‑1 (MQ=255)
                                                                                                                          gCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGAt                                                                                                   >  2:2174430/1‑71 (MQ=255)
                                                                                                                           cccGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGCGGATGGCCGCCCCGCCTACCGATCGTCGGATGTCGCGATc                   >  2:528622/1‑150 (MQ=255)
                                                                                                                            ccGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAgg                                                                                              <  2:245219/74‑1 (MQ=255)
                                                                                                                            ccGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAgg                                                                                              >  1:245219/1‑74 (MQ=255)
                                                                                                                              gCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAgt                >  2:1610277/1‑150 (MQ=37)
                                                                                                                              gCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGAtc                                   <  1:724662/131‑1 (MQ=255)
                                                                                                                              gCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGAtc                                   >  2:724662/1‑131 (MQ=255)
                                                                                                                                  cTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTgg            <  1:1338911/150‑1 (MQ=255)
                                                                                                                                     cAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGgc                                                   >  2:1123010/1‑108 (MQ=255)
                                                                                                                                     cAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGgc                                                   <  1:1123010/108‑1 (MQ=255)
                                                                                                                                      aGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGGGTGGGCGt        >  1:131709/1‑150 (MQ=255)
                                                                                                                                           cTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCg   <  2:1968387/150‑1 (MQ=255)
                                                                                                                                              tgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTAt                                                                            >  1:1409932/1‑74 (MQ=255)
                                                                                                                                              tgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTAt                                                                            <  2:1409932/74‑1 (MQ=255)
                                                                                                                                              tgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCgg  <  1:789445/148‑1 (MQ=255)
                                                                                                                                              tgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCgg  >  2:789445/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGG  >  NC_002947/357951‑358240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑