Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,741,284	A→G	13.2%	intergenic (+475/+277)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,284	0	A	G	13.2%	172.8 / 14.3	68	intergenic (+475/+277)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (28/31);  new base G (5/4);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CC‑TCC‑GCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAC  >  NC_002947/4741155‑4741409                                                                                                                                      |                                                                                                                                gTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGaatccag                                                                                                             <  1:1708476/150‑6 (MQ=255)              ccGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCgg                                                                                               <  2:2073021/150‑1 (MQ=255)               cGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGa                                                                                              >  1:1357010/1‑150 (MQ=255)                gCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGa                                                                                              >  1:2187782/1‑149 (MQ=255)                gCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGa                                                                                              <  2:2187782/149‑1 (MQ=255)                gCCTCCTGGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAT‑CA‑GC‑TTC‑TGCGACTTGCATTCGTGAA‑TCGAGGCACAGGTCCCGGac                                                                                             >  2:598509/1‑150 (MQ=255)                  ctcctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCCCCGTCTATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGa                                                                                              <  1:1952974/147‑1 (MQ=255)                  ctcctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGa                                                                                              >  2:1952974/1‑147 (MQ=255)                          tGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTcctcct                                                                                   <  1:1693817/150‑1 (MQ=255)                            gTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCCCAGGCCCCGGACACGTCCTCCTGc                                                                                 <  2:906137/150‑1 (MQ=255)                                 aaTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGa                                                                                              >  2:1411754/1‑132 (MQ=255)                                 aaTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGa                                                                                              <  1:1411754/132‑1 (MQ=255)                                     tgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCg                                                                        >  1:1217161/1‑150 (MQ=255)                                     tgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCg                                                                        >  2:2144728/1‑150 (MQ=255)                                           aCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg                                                                  <  1:1304368/150‑1 (MQ=255)                                           aCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg                                                                  >  2:1101619/1‑150 (MQ=255)                                            cGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTgg                                                                 <  2:1451298/150‑1 (MQ=255)                                             ggAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGTTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGACTCGACCTTCGc                                                                >  2:250916/1‑150 (MQ=255)                                                             aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATc                                                >  2:732192/1‑150 (MQ=255)                                                              ccGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CC‑TCT‑GCGACT‑TGCATTCGt                                                                                                                     <  1:1637391/81‑1 (MQ=255)                                                                ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg                                                                  >  1:1011619/1‑129 (MQ=255)                                                                ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg                                                                  <  2:1011619/129‑1 (MQ=255)                                                                ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                             >  2:1196805/1‑150 (MQ=255)                                                                 ggcATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTCGCCAGCGGCGCCTTGATCACgg                                            <  2:2286500/148‑1 (MQ=255)                                                                  ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACggg                                           >  2:1175744/1‑149 (MQ=255)                                                                   cATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg                                                                  <  2:1654182/126‑1 (MQ=255)                                                                   cATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg                                                                  >  1:1654182/1‑126 (MQ=255)                                                                    aTGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGccc                                                                                                  <  2:2401122/93‑1 (MQ=255)                                                                        cGTATTGAACTGGGTATAGTTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACATGCCCCGTACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCCCAcc                                     <  2:1021797/150‑1 (MQ=255)                                                                        cGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACAcc                                     >  1:747315/1‑150 (MQ=255)                                                                          tATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                   >  1:814738/1‑150 (MQ=255)                                                                          tATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                   <  2:1095791/150‑1 (MQ=255)                                                                            ttGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                 <  1:983223/150‑1 (MQ=255)                                                                              gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                               <  1:2144728/150‑1 (MQ=255)                                                                              gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                               <  1:178097/150‑1 (MQ=255)                                                                              gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                               >  1:454025/1‑150 (MQ=255)                                                                               aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                              >  1:1839477/1‑150 (MQ=255)                                                                               aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                              >  2:1858953/1‑150 (MQ=255)                                                                                 ccggggATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                            <  1:121060/148‑1 (MQ=255)                                                                                 cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                            >  2:1170795/1‑150 (MQ=255)                                                                                 cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                            <  1:2096064/134‑1 (MQ=255)                                                                                 cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                            >  2:2096064/1‑134 (MQ=255)                                                                                  tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                           <  2:747315/150‑1 (MQ=255)                                                                                   gggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                          <  2:2247841/150‑1 (MQ=255)                                                                                    ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                         >  1:1720218/1‑150 (MQ=255)                                                                                          ggTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                                  <  1:1710530/119‑1 (MQ=255)                                                                                          ggTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                                  >  2:1710530/1‑119 (MQ=255)                                                                                          ggTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                   <  1:311278/150‑1 (MQ=255)                                                                                           gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg                                                                  <  1:1848647/102‑1 (MQ=255)                                                                                           gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg                                                                  >  2:1848647/1‑102 (MQ=255)                                                                                           gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                           <  2:1567653/141‑1 (MQ=255)                                                                                           gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                           >  1:1567653/1‑141 (MQ=255)                                                                                           gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGAGCCTCGACCTTGTCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                  <  1:903847/150‑1 (MQ=255)                                                                                           gTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                  >  2:715712/1‑150 (MQ=255)                                                                                           gTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                  >  1:75369/1‑150 (MQ=255)                                                                                            tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACCCCGCGATAGGCCTTGTACTTgg                 >  2:1491777/1‑150 (MQ=255)                                                                                              cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAc               <  1:1175744/150‑1 (MQ=255)                                                                                                 ctctGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCg            >  1:368712/1‑150 (MQ=255)                                                                                                    tGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGcc         >  1:528202/1‑150 (MQ=255)                                                                                                    tGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGcc         <  1:2307220/150‑1 (MQ=255)                                                                                                     ggTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGAc                                                                      >  2:991212/1‑88 (MQ=255)                                                                                                     ggTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGAc                                                                      <  1:991212/88‑1 (MQ=255)                                                                                                      gTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAc       <  2:1357010/150‑1 (MQ=255)                                                                                                      gTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCATGGGTCGCCTCGACCTTGGACAGAGCCGCCTGAAAGACGTGCTCGCCGCGATAGGCCTTGTACTTGGACCCGGCCAc       >  1:541284/1‑150 (MQ=255)                                                                                                         aCCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                                  <  1:1971198/104‑1 (MQ=255)                                                                                                           cTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAc       <  1:510441/145‑1 (MQ=255)                                                                                                           cTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                               <  2:1111074/121‑1 (MQ=255)                                                                                                           cTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTTGCATTCGTGAA‑TCCAGGCACAGTCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc  >  1:7007/1‑150 (MQ=255)                                                                                                                                      |                                                                                                                                GTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CC‑TCC‑GCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAC  >  NC_002947/4741155‑4741409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑