Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	377,527	A→G	5.2%	R67G (AGG→GGG)	PP_0314 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	377,527	0	A	G	5.2%	269.2 / 2.2	88	R67G (AGG→GGG)	PP_0314	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (44/39);  new base G (2/3);  total (46/42)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

GATATCACGAGCCTCGCGCCTACTCGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCGCGCCAGTCCGAAACCTTGTCACGGGA  >  NC_002947/377381‑377676                                                                                                                                                   |                                                                                                                                                      gATATCACGAGCCTCGCGCCTACTCGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGgc                                                                                                                                                    <  1:932270/150‑1 (MQ=255)    atCACGAGCCTCGCGCCTACTCGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGGCTCGGgcggt                                                                                                                                                 <  2:2260511/150‑3 (MQ=255)       aCGAGCCTCGCGCCTACTCGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCa                                                                                                                                              >  1:2312455/1‑150 (MQ=255)         gAGCCTCGCGCCTACTCGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATa                                                                                                                                            <  1:1980283/150‑1 (MQ=255)             cTCGCGCCTACTCGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGcc                                                                                                                                        >  2:539005/1‑150 (MQ=255)             cTCGCGCCTACTCGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGAGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGcc                                                                                                                                        >  2:1612638/1‑150 (MQ=255)              tCGCGCCTACTCGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGccc                                                                                                                                       >  1:2039879/1‑150 (MQ=255)                    ctactaGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGt                                                                                                                                 >  1:275573/1‑150 (MQ=255)                    cTACTCGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGt                                                                                                                                 <  2:87551/150‑1 (MQ=255)                      aCTCGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGc                                                                                                                               <  2:2065637/150‑1 (MQ=255)                          gCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCt                                                                                                                                      <  1:89463/139‑1 (MQ=255)                          gCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCt                                                                                                                                      >  2:89463/1‑139 (MQ=255)                                cGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGtt                                                                                                                     <  2:2312455/150‑1 (MQ=255)                                            tgtAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAgggggg                                                                                                         <  1:704913/150‑1 (MQ=255)                                             gtAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAggggggg                                                                                                        >  2:358053/1‑150 (MQ=255)                                                gTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTc                                                                                                     >  1:898083/1‑150 (MQ=255)                                                gTCCCGGCATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTAATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTc                                                                                                     >  1:397907/1‑150 (MQ=255)                                                 tCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCa                                                                                                    >  2:992740/1‑150 (MQ=255)                                                  cccGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAg                                                                                                                                                      <  2:433136/99‑1 (MQ=255)                                                  cccGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAg                                                                                                                                                      >  1:433136/1‑99 (MQ=255)                                                   ctggTATCAGGGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAAt                                                                                                  <  2:539932/148‑1 (MQ=255)                                                   ccGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAAt                                                                                                  >  2:1804717/1‑150 (MQ=255)                                                    cGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAg                                                                                                                                                      <  2:1066076/97‑1 (MQ=255)                                                    cGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAg                                                                                                                                                      >  1:1066076/1‑97 (MQ=255)                                                        aTCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTa                                                                                             <  2:406957/150‑1 (MQ=255)                                                         tCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTAc                                                                                            <  2:898083/150‑1 (MQ=255)                                                                  gCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGc                                                                                   <  1:1678884/150‑1 (MQ=255)                                                                   cTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCTGAGGCTCGGgcggt                                                                                                                                                 >  2:919966/1‑85 (MQ=255)                                                                   cTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCTGAGGCTCGGgcggt                                                                                                                                                 <  1:919966/87‑3 (MQ=255)                                                                    tttCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCa                                                                                 >  2:1413447/1‑150 (MQ=255)                                                                    tttCTGTGGCCAGCACCTGGATCCGTTGACAAGTAGCTAAGTCATGTACGTGTGCGGTCGCTTCGACCGCCCGCGACTTGCACAGCTGATAACCGCTGGTGTGCTAAGCCCGTTCGGAAAGGGGGGGCTAAAAGATTACGCCTACTGCCa                                                                                 >  1:1151161/1‑150 (MQ=255)                                                                     ttCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTa                                                                                       >  1:502231/1‑143 (MQ=255)                                                                     ttCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTa                                                                                       <  2:502231/143‑1 (MQ=255)                                                                        tgtgGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGt                                                                             >  1:644498/1‑150 (MQ=255)                                                                         gtgGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCGTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTg                                                                            <  2:1151161/150‑1 (MQ=255)                                                                            gCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGAcc                                                                         >  2:722344/1‑150 (MQ=255)                                                                            gCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGAcc                                                                         <  1:956051/150‑1 (MQ=255)                                                                             ccAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGAccc                                                                        >  1:1821100/1‑150 (MQ=255)                                                                                    cTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAAc                                                                 >  1:2354733/1‑150 (MQ=255)                                                                                      ggATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAAt                                                                                                  >  1:243865/1‑115 (MQ=255)                                                                                      ggATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAAt                                                                                                  <  2:243865/115‑1 (MQ=255)                                                                                      ggATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACtt                                                               <  2:2354733/150‑1 (MQ=255)                                                                                        aTCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTg                                                             <  2:275573/150‑1 (MQ=255)                                                                                         tCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACttt                                                              <  2:1640153/148‑1 (MQ=255)                                                                                         tCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACttt                                                              >  1:1640153/1‑148 (MQ=255)                                                                                         tCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGc                                                            <  1:1612638/150‑1 (MQ=255)                                                                                          ccGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCt                                                           <  2:644498/150‑1 (MQ=255)                                                                                            gTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGa                                                         >  1:1264965/1‑150 (MQ=255)                                                                                             ttGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGAc                                                        >  1:793297/1‑150 (MQ=255)                                                                                             ttGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGAc                                                        >  2:1096188/1‑150 (MQ=255)                                                                                                aCAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCAt                                                                                                                                             <  1:2258269/62‑1 (MQ=255)                                                                                                aCAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCAt                                                                                                                                             >  2:2258269/1‑62 (MQ=255)                                                                                                  aGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAg                                                   <  1:992740/150‑1 (MQ=255)                                                                                                  aGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCCGTCAACTTTGCTGACCCTAg                                                   <  2:793297/150‑1 (MQ=255)                                                                                                    gTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCaa                                                                                                   >  1:1001888/1‑100 (MQ=255)                                                                                                    gTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCaa                                                                                                   <  2:1001888/100‑1 (MQ=255)                                                                                                    gTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTg                                                 >  1:2142173/1‑150 (MQ=255)                                                                                                     tAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTgg                                                >  2:686984/1‑150 (MQ=255)                                                                                                        cTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCg                                             >  1:1898264/1‑150 (MQ=255)                                                                                                        cTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCg                                             >  2:551683/1‑150 (MQ=255)                                                                                                         tATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGt                                            <  2:2039879/150‑1 (MQ=255)                                                                                                          aTCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGt                                            <  2:510362/149‑1 (MQ=255)                                                                                                          aTCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGt                                            >  1:510362/1‑149 (MQ=255)                                                                                                             cATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTcc                                        >  2:2356682/1‑150 (MQ=255)                                                                                                             cATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGATTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGt                                                                             <  2:1973961/113‑1 (MQ=255)                                                                                                             cATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGATTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGt                                                                             >  1:1973961/1‑113 (MQ=255)                                                                                                               ttGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTcccc                                      <  2:1821100/150‑1 (MQ=255)                                                                                                               gtgttcAATTGCCATCGCAGCTACCGCCCCAGCCTAAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTcccc                                      <  2:397907/145‑1 (MQ=255)                                                                                                                   gCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTccc                                       <  2:1385829/145‑1 (MQ=255)                                                                                                                   gCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTccc                                       >  1:1385829/1‑145 (MQ=255)                                                                                                                   gCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCaa                                  >  2:134524/1‑150 (MQ=255)                                                                                                                     aaTGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCt                                                                       >  1:2021534/1‑111 (MQ=255)                                                                                                                     aaTGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCt                                                                       <  2:2021534/111‑1 (MQ=255)                                                                                                                       tGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAatcat                              <  1:722344/150‑1 (MQ=255)                                                                                                                       tGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAatcat                              <  1:686984/150‑1 (MQ=255)                                                                                                                        ggCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAatcatc                             <  2:2019008/150‑1 (MQ=255)                                                                                                                           cATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATcgcg                          >  1:619921/1‑150 (MQ=255)                                                                                                                            aTCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCgcgc                         >  1:2048423/1‑150 (MQ=255)                                                                                                                            aTCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCgcgc                         <  1:1096188/150‑1 (MQ=255)                                                                                                                            aTCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCgcgc                         >  2:2078586/1‑150 (MQ=255)                                                                                                                             tCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACACTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCGCGcc                        >  1:1470158/1‑150 (MQ=255)                                                                                                                                 ttCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCGCGCCAGTc                    >  1:1082114/1‑150 (MQ=255)                                                                                                                                      ccGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCCTCGCGCCAGTCCGaaa               >  2:1868877/1‑150 (MQ=255)                                                                                                                                         cccGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAACGCGGGGGCTCAATGCTTACGCCTACTCCCAAGGTGACCCTGTCAACTGTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCGCGCCAGTCCGAAACCt            >  2:573267/1‑150 (MQ=255)                                                                                                                                          ctgAGGCTCGGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCGCGCCAGGCCGAAACCtt           <  2:832997/148‑1 (MQ=255)                                                                                                                                                cTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCGCGCCAGTCCGAAACCTTGTCACg     >  2:1243616/1‑150 (MQ=255)                                                                                                                                                 tCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCGCGCCAGTCCGAAACCTTGTCACgg    <  1:134524/150‑1 (MQ=255)                                                                                                                                                 tCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCGCGCCAGTCCGAAACCTTGTCACgg    <  1:1804717/150‑1 (MQ=255)                                                                                                                                                   aGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCGCGCCAGTCCGAAACCTTGTCACGGGa  <  2:2142173/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GATATCACGAGCCTCGCGCCTACTCGCCGTACGGTGGCATGCGTGTAGTCCCGGTATCAGCGCTGGCTTTCTGTGGCCAGCACCTGGATCCGTTGACAGGTAGCTATCCATTGGGCAATGGCCATCGCTTCTACCGCCCGAGCCTCAGGCGCTTCATAAGCCCTGACGTGCTAAGCCCGTTCGGAAAGGGGGGGCTCAATGCTTACGCCTACTGCCAAGGTGACCCTGTCAACTTTGCTGACCCTAGTGGTCGTTTCCCCGCAATCATCGCGCCAGTCCGAAACCTTGTCACGGGA  >  NC_002947/377381‑377676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑