Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,744,788 C→G 9.5% S28T (AGT→ACT)  PP_4198 ← OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,744,7880CG 9.5% 322.5 / 22.1 105S28T (AGT→ACT) PP_4198OmpA family protein
Reads supporting (aligned to +/- strand):  ref base C (54/41);  new base G (5/5);  total (59/46)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01

Only 100 of 109 total aligned reads displayed.

ATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTGCTCTTCG  >  NC_002947/4744640‑4744936
                                                                                                                                                    |                                                                                                                                                    
aTCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCAct                                                                                                                                                     >  1:2344462/1‑150 (MQ=255)
   ggAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActgct                                                                                                                                                  <  1:1997542/150‑1 (MQ=255)
   ggAACCGGTACACATATAACGGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActgct                                                                                                                                                  >  2:712148/1‑150 (MQ=255)
        cGGTGCCGATGTAACTGGACAGGCGCTTTGCCCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCt                                                                                                                                             <  1:1811163/150‑1 (MQ=255)
            gCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACt                                                                                                                                         >  1:1293113/1‑150 (MQ=255)
              cGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGAcggc                                                                                                                                       >  1:1508658/1‑147 (MQ=255)
              cGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTgg                                                                                                                                       >  1:1102004/1‑150 (MQ=255)
              cGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTgg                                                                                                                                       >  1:1102023/1‑150 (MQ=255)
               gATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgc                                                                                                                                      >  1:1646943/1‑150 (MQ=255)
                aTGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTggatgagg                                                                                                                                     <  2:1545776/150‑8 (MQ=255)
                 tGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgcgc                                                                                                                                    >  2:379048/1‑150 (MQ=255)
                  gTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCa                                                                                                                                   >  1:1606177/1‑150 (MQ=255)
                    aaCTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAAc                                                                                                                                 >  2:476131/1‑150 (MQ=255)
                      cTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAGTTGCCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCt                                                                                                                               >  1:1620963/1‑150 (MQ=255)
                       tGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCtt                                                                                                                              >  2:1010692/1‑150 (MQ=255)
                       tGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGCCCTACTCTTTGAACCCCTTCAGGGGNANTGGGTCNGCGTGCAGCGCGNCACTGCTGCGCTGACTGGCGCAACNtt                                                                                                                              <  1:1252597/150‑1 (MQ=255)
                        ggACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTg                                                                                                                             <  1:1010692/150‑1 (MQ=255)
                               cgcTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCt                                                                                                                      <  2:1293113/150‑1 (MQ=255)
                               cgcTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCt                                                                                                                      <  2:1813198/150‑1 (MQ=255)
                                  tCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGcc                                                                                                                   >  2:1116337/1‑150 (MQ=255)
                                             cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAg                                                                                                        >  1:216432/1‑150 (MQ=255)
                                             cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGAGCGCGTGCAAACCTGCCGTCAGCGCCAg                                                                                                        >  1:611139/1‑150 (MQ=255)
                                             cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCAAATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTggatga                                                                                                                                       <  2:1538685/119‑6 (MQ=255)
                                             cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCAAATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTggatga                                                                                                                                       >  1:1538685/1‑114 (MQ=255)
                                              aGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGc                                                                                                       >  2:1966373/1‑150 (MQ=255)
                                               gCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgc                                                                                                                                      <  1:740713/118‑1 (MQ=255)
                                               gCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgc                                                                                                                                      >  2:740713/1‑118 (MQ=255)
                                                cGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCaa                                                                                                     <  2:1646943/150‑1 (MQ=255)
                                                      aCCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGAACTGATGCTTTCACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAg                                                                                               >  2:1871379/1‑150 (MQ=255)
                                                       cccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACGCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTCCGCGGACGGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGt                                                                                              <  1:1641907/150‑1 (MQ=255)
                                                       cccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGt                                                                                              <  1:1897623/150‑1 (MQ=255)
                                                       cccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGt                                                                                              <  2:145644/150‑1 (MQ=255)
                                                       cccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGt                                                                                              <  1:614640/150‑1 (MQ=255)
                                                        ccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACGCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCAGTAAGCACCAGCAATGCCAGTg                                                                                             >  2:1641907/1‑150 (MQ=255)
                                                        ccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTg                                                                                                                             <  1:922696/118‑1 (MQ=255)
                                                        ccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTg                                                                                             <  2:747110/150‑1 (MQ=255)
                                                          gcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCCACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTgga                                                                                                                                          <  1:1952147/103‑3 (MQ=255)
                                                          gcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTgga                                                                                                                                          >  2:1952147/1‑101 (MQ=255)
                                                              gAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCt                                                                                                                      >  1:1856571/1‑119 (MQ=255)
                                                              gAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCt                                                                                                                      <  2:1856571/119‑1 (MQ=255)
                                                                 tcacgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAg                                                                                    <  2:2344462/150‑1 (MQ=255)
                                                                 tcacgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAg                                                                                    <  2:419344/150‑1 (MQ=255)
                                                                 tcacgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCAGGCGATGCCAGCAATGCCAGTGCAGCCAAAg                                                                                    <  1:1871379/150‑1 (MQ=255)
                                                                   acgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGTAGCCAAAGGc                                                                                  >  1:1101615/1‑150 (MQ=255)
                                                                     gtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGt                                                                                >  2:362701/1‑150 (MQ=255)
                                                                          gcggcgCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATTCTAGTTAAGCCAAAGGCGTacgac                                                                           <  2:2301058/150‑1 (MQ=255)
                                                                              cgcgcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGAt                                                                       <  2:216432/150‑1 (MQ=255)
                                                                                 gcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCAt                                                                    <  2:1102023/150‑1 (MQ=255)
                                                                                 gcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCAt                                                                    <  2:1102004/150‑1 (MQ=255)
                                                                                  cTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATg                                                                   <  2:793026/150‑1 (MQ=255)
                                                                                   tGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATgg                                                                  >  2:1066664/1‑150 (MQ=255)
                                                                                   tGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATgg                                                                  <  1:209884/150‑1 (MQ=255)
                                                                                      gcagcaCATCGGAATCATCTTTGAACCGCTGGAAGGCAACGGTGAACTCATACAGCGCGGCACTGCTGCGCTGACTGGCGCAACCGTGCCAACCTGCCGTCGTCGCCAGCAATGCCCGTGCATCCAAAGGCGTACGACGGATCCTAGCTg                                                               >  2:770817/1‑150 (MQ=255)
                                                                                             aTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTcc                                                        >  2:132713/1‑150 (MQ=255)
                                                                                             aTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTcc                                                        >  1:278426/1‑150 (MQ=255)
                                                                                             aTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTg                                                                                                                                            >  2:1200732/1‑66 (MQ=38)
                                                                                             aTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTg                                                                                                                                            <  1:1200732/66‑1 (MQ=38)
                                                                                              tCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCa                                                                                                <  1:100140/109‑1 (MQ=255)
                                                                                              tCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCa                                                                                                >  2:100140/1‑109 (MQ=255)
                                                                                                 gAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGCTCATGGCTGCACCTCCAGtt                                                    >  2:744805/1‑150 (MQ=255)
                                                                                                 gAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGtt                                                    <  1:1066664/150‑1 (MQ=255)
                                                                                                   atcatcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgc                                                  <  2:1606177/150‑1 (MQ=255)
                                                                                                     catcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgctt                                                >  1:1572592/1‑150 (MQ=255)
                                                                                                      atcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTgg                                                                                                                                       >  2:1319662/1‑62 (MQ=255)
                                                                                                      atcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCAc                                                            <  2:216989/137‑1 (MQ=255)
                                                                                                      atcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCAc                                                            >  1:216989/1‑137 (MQ=255)
                                                                                                       tcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCCATGCCAGTGCAGCCAAAGACGTACGACGGATCATGGCTGCACCTCCAGttgcttgc                                              >  2:571412/1‑150 (MQ=255)
                                                                                                         tttGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCaaa                                                                                                                         >  2:197869/1‑73 (MQ=255)
                                                                                                         tttGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCaaa                                                                                                                         <  1:197869/73‑1 (MQ=255)
                                                                                                         tttGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCa                                                                                                         >  2:2088167/1‑89 (MQ=255)
                                                                                                         tttGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTgcgc                                            >  1:245936/1‑150 (MQ=255)
                                                                                                               ccccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAgcgc                                      >  2:920298/1‑150 (MQ=255)
                                                                                                               ccccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTGGCGCaacagg                                      >  2:633547/1‑146 (MQ=255)
                                                                                                                cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgctt                                                >  1:1740675/1‑139 (MQ=255)
                                                                                                                cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                     <  1:476131/150‑1 (MQ=255)
                                                                                                                cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                     >  1:508485/1‑150 (MQ=255)
                                                                                                                cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                     >  1:1700921/1‑150 (MQ=255)
                                                                                                                cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGCCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgctt                                                <  2:1740675/139‑1 (MQ=255)
                                                                                                                 ccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCtt                                    <  1:1986327/150‑1 (MQ=255)
                                                                                                                        ggCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCC‑AAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCcgcg                            >  1:1478672/1‑150 (MQ=255)
                                                                                                                         gCAATGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCcgcg                            >  1:2056491/1‑150 (MQ=255)
                                                                                                                               gTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTgca                      <  2:745116/150‑1 (MQ=255)
                                                                                                                                tGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTgcag                     >  2:1374829/1‑150 (MQ=255)
                                                                                                                                tGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTgcag                     <  1:1116337/150‑1 (MQ=255)
                                                                                                                                   ccTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCAc                  >  2:847311/1‑150 (MQ=255)
                                                                                                                                   ccTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCAc                  >  1:870082/1‑150 (MQ=255)
                                                                                                                                   ccTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCAc                  >  1:1367963/1‑150 (MQ=255)
                                                                                                                                   ccTCATCCAGCGCGGCACTGCTCCCCTGACTGGCGCAACCTCGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAATGCGTACGACGGATCATGGCTGCACCTATAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCAc                  <  1:1820115/150‑1 (MQ=255)
                                                                                                                                    cTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATgg                                                                  >  1:1078265/1‑101 (MQ=255)
                                                                                                                                    cTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATgg                                                                  <  2:1078265/101‑1 (MQ=255)
                                                                                                                                    cTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCGAGCGCTTGACCCGCGACTGCAGCGcc                 >  2:394252/1‑150 (MQ=255)
                                                                                                                                    cTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCAcc                 >  1:821730/1‑150 (MQ=255)
                                                                                                                                       agtcAGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGc              >  2:429896/4‑150 (MQ=255)
                                                                                                                                       aTCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGtt                                                    <  1:1170383/112‑1 (MQ=255)
                                                                                                                                       aTCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGtt                                                    >  2:1170383/1‑112 (MQ=255)
                                                                                                                                        tCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCa             >  2:1255192/1‑150 (MQ=255)
                                                                                                                                        gtcAGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGa                                                                        <  1:1884416/89‑1 (MQ=255)
                                                                                                                                        gtcAGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGa                                                                        >  2:1884416/3‑91 (MQ=255)
                                                                                                                                         ccAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGTAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCATCACCTGCAg            <  2:1101615/150‑1 (MQ=255)
                                                                                                                                                   aCTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTgctcttcg  <  2:784392/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTGCTCTTCG  >  NC_002947/4744640‑4744936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑