Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,744,792 T→C 9.5% S27G (AGC→GGC)  PP_4198 ← OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,744,7920TC 9.5% 293.4 / 16.5 105S27G (AGC→GGC) PP_4198OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (54/41);  new base C (5/5);  total (59/46)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01

Only 100 of 108 total aligned reads displayed.

GGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTGCTCTTCGCTC  >  NC_002947/4744643‑4744939
                                                                                                                                                     |                                                                                                                                                   
ggAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActgct                                                                                                                                                     <  1:1997542/150‑1 (MQ=255)
ggAACCGGTACACATATAACGGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActgct                                                                                                                                                     >  2:712148/1‑150 (MQ=255)
     cGGTGCCGATGTAACTGGACAGGCGCTTTGCCCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCt                                                                                                                                                <  1:1811163/150‑1 (MQ=255)
         gCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACt                                                                                                                                            >  1:1293113/1‑150 (MQ=255)
           cGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGAcggc                                                                                                                                          >  1:1508658/1‑147 (MQ=255)
           cGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTgg                                                                                                                                          >  1:1102004/1‑150 (MQ=255)
           cGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTgg                                                                                                                                          >  1:1102023/1‑150 (MQ=255)
            gATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgc                                                                                                                                         >  1:1646943/1‑150 (MQ=255)
             aTGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTggatgagg                                                                                                                                        <  2:1545776/150‑8 (MQ=255)
              tGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgcgc                                                                                                                                       >  2:379048/1‑150 (MQ=255)
               gTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCa                                                                                                                                      >  1:1606177/1‑150 (MQ=255)
                   cTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAGTTGCCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCt                                                                                                                                  >  1:1620963/1‑150 (MQ=255)
                    tGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGCCCTACTCTTTGAACCCCTTCAGGGGNANTGGGTCNGCGTGCAGCGCGNCACTGCTGCGCTGACTGGCGCAACNtt                                                                                                                                 <  1:1252597/150‑1 (MQ=255)
                     ggACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTg                                                                                                                                <  1:1010692/150‑1 (MQ=255)
                            cgcTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCt                                                                                                                         <  2:1293113/150‑1 (MQ=255)
                            cgcTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCt                                                                                                                         <  2:1813198/150‑1 (MQ=255)
                               tCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGcc                                                                                                                      >  2:1116337/1‑150 (MQ=255)
                                          cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAg                                                                                                           >  1:216432/1‑150 (MQ=255)
                                          cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGAGCGCGTGCAAACCTGCCGTCAGCGCCAg                                                                                                           >  1:611139/1‑150 (MQ=255)
                                          cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCAAATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTggatga                                                                                                                                          >  1:1538685/1‑114 (MQ=255)
                                          cAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCAAATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTggatga                                                                                                                                          <  2:1538685/119‑6 (MQ=255)
                                           aGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGc                                                                                                          >  2:1966373/1‑150 (MQ=255)
                                            gCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgc                                                                                                                                         <  1:740713/118‑1 (MQ=255)
                                            gCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgc                                                                                                                                         >  2:740713/1‑118 (MQ=255)
                                             cGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCaa                                                                                                        <  2:1646943/150‑1 (MQ=255)
                                                   aCCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGAACTGATGCTTTCACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAg                                                                                                  >  2:1871379/1‑150 (MQ=255)
                                                    cccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACGCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTCCGCGGACGGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGt                                                                                                 <  1:1641907/150‑1 (MQ=255)
                                                    cccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGt                                                                                                 <  1:614640/150‑1 (MQ=255)
                                                    cccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGt                                                                                                 <  2:145644/150‑1 (MQ=255)
                                                    cccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGt                                                                                                 <  1:1897623/150‑1 (MQ=255)
                                                     ccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACGCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCAGTAAGCACCAGCAATGCCAGTg                                                                                                >  2:1641907/1‑150 (MQ=255)
                                                     ccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTg                                                                                                                                <  1:922696/118‑1 (MQ=255)
                                                     ccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTg                                                                                                <  2:747110/150‑1 (MQ=255)
                                                       gcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCCACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTgga                                                                                                                                             <  1:1952147/103‑3 (MQ=255)
                                                           gAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCt                                                                                                                         <  2:1856571/119‑1 (MQ=255)
                                                           gAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCt                                                                                                                         >  1:1856571/1‑119 (MQ=255)
                                                           gAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCa                                                                                          >  2:1641618/1‑150 (MQ=255)
                                                              tcacgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAg                                                                                       <  2:419344/150‑1 (MQ=255)
                                                              tcacgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAg                                                                                       <  2:2344462/150‑1 (MQ=255)
                                                              tcacgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCAGGCGATGCCAGCAATGCCAGTGCAGCCAAAg                                                                                       <  1:1871379/150‑1 (MQ=255)
                                                                acgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGTAGCCAAAGGc                                                                                     >  1:1101615/1‑150 (MQ=255)
                                                                  gtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGt                                                                                   >  2:362701/1‑150 (MQ=255)
                                                                       gcggcgCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATTCTAGTTAAGCCAAAGGCGTacgac                                                                              <  2:2301058/150‑1 (MQ=255)
                                                                           cgcgcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGAt                                                                          <  2:216432/150‑1 (MQ=255)
                                                                              gcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCAt                                                                       <  2:1102023/150‑1 (MQ=255)
                                                                              gcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCAt                                                                       <  2:1102004/150‑1 (MQ=255)
                                                                               cTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATg                                                                      <  2:793026/150‑1 (MQ=255)
                                                                                tGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATgg                                                                     >  2:1066664/1‑150 (MQ=255)
                                                                                tGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATgg                                                                     <  1:209884/150‑1 (MQ=255)
                                                                                   gcagcaCATCGGAATCATCTTTGAACCGCTGGAAGGCAACGGTGAACTCATACAGCGCGGCACTGCTGCGCTGACTGGCGCAACCGTGCCAACCTGCCGTCGTCGCCAGCAATGCCCGTGCATCCAAAGGCGTACGACGGATCCTAGCTg                                                                  >  2:770817/1‑150 (MQ=255)
                                                                                          aTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTcc                                                           >  1:278426/1‑150 (MQ=255)
                                                                                          aTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTcc                                                           >  2:132713/1‑150 (MQ=255)
                                                                                          aTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTg                                                                                                                                               >  2:1200732/1‑66 (MQ=38)
                                                                                          aTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTg                                                                                                                                               <  1:1200732/66‑1 (MQ=38)
                                                                                           tCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCa                                                                                                   >  2:100140/1‑109 (MQ=255)
                                                                                           tCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCa                                                                                                   <  1:100140/109‑1 (MQ=255)
                                                                                           tCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCa                                                          >  2:1159934/1‑150 (MQ=255)
                                                                                              gAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGCTCATGGCTGCACCTCCAGtt                                                       >  2:744805/1‑150 (MQ=255)
                                                                                              gAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGtt                                                       <  1:1066664/150‑1 (MQ=255)
                                                                                                atcatcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgc                                                     <  2:1606177/150‑1 (MQ=255)
                                                                                                  catcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgctt                                                   >  1:1572592/1‑150 (MQ=255)
                                                                                                   atcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTgg                                                                                                                                          <  1:1319662/62‑1 (MQ=255)
                                                                                                   atcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTgg                                                                                                                                          >  2:1319662/1‑62 (MQ=255)
                                                                                                   atcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCAc                                                               >  1:216989/1‑137 (MQ=255)
                                                                                                   atcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCAc                                                               <  2:216989/137‑1 (MQ=255)
                                                                                                    tcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCCATGCCAGTGCAGCCAAAGACGTACGACGGATCATGGCTGCACCTCCAGttgcttgc                                                 >  2:571412/1‑150 (MQ=255)
                                                                                                      tttGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCaaa                                                                                                                            >  2:197869/1‑73 (MQ=255)
                                                                                                      tttGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCaaa                                                                                                                            <  1:197869/73‑1 (MQ=255)
                                                                                                      tttGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCa                                                                                                            <  1:2088167/89‑1 (MQ=255)
                                                                                                      tttGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCa                                                                                                            >  2:2088167/1‑89 (MQ=255)
                                                                                                          aCCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAgc                                           >  2:2162237/1‑150 (MQ=255)
                                                                                                            ccccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAgcgc                                         >  2:920298/1‑150 (MQ=255)
                                                                                                            ccccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTGGCGCaacagg                                         >  2:633547/1‑146 (MQ=255)
                                                                                                             cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgctt                                                   >  1:1740675/1‑139 (MQ=255)
                                                                                                             cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                        <  1:476131/150‑1 (MQ=255)
                                                                                                             cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                        >  1:1700921/1‑150 (MQ=255)
                                                                                                             cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                        >  1:508485/1‑150 (MQ=255)
                                                                                                             cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGCCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgctt                                                   <  2:1740675/139‑1 (MQ=255)
                                                                                                              ccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCtt                                       <  1:1986327/150‑1 (MQ=255)
                                                                                                                tGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGAGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGa                                     <  1:773935/150‑1 (MQ=255)
                                                                                                                     ggCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCC‑AAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCcgcg                               >  1:1478672/1‑150 (MQ=255)
                                                                                                                      gCAATGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCcgcg                               >  1:2056491/1‑150 (MQ=255)
                                                                                                                        aaCGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGAc                             >  1:1792427/1‑150 (MQ=255)
                                                                                                                            gTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTgca                         <  2:745116/150‑1 (MQ=255)
                                                                                                                             tGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTgcag                        <  1:1116337/150‑1 (MQ=255)
                                                                                                                                ccTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCAc                     >  1:870082/1‑150 (MQ=255)
                                                                                                                                ccTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCAc                     >  2:847311/1‑150 (MQ=255)
                                                                                                                                 cTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATgg                                                                     >  1:1078265/1‑101 (MQ=255)
                                                                                                                                 cTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATgg                                                                     <  2:1078265/101‑1 (MQ=255)
                                                                                                                                 cTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCGAGCGCTTGACCCGCGACTGCAGCGcc                    >  2:394252/1‑150 (MQ=255)
                                                                                                                                 cTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCAcc                    >  1:821730/1‑150 (MQ=255)
                                                                                                                                    agtcAGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGc                 >  2:429896/4‑150 (MQ=255)
                                                                                                                                    aTCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGtt                                                       >  2:1170383/1‑112 (MQ=255)
                                                                                                                                    aTCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGtt                                                       <  1:1170383/112‑1 (MQ=255)
                                                                                                                                     tCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCa                >  2:1255192/1‑150 (MQ=255)
                                                                                                                                     gtcAGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGa                                                                           >  2:1884416/3‑91 (MQ=255)
                                                                                                                                     gtcAGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGa                                                                           <  1:1884416/89‑1 (MQ=255)
                                                                                                                                      ccAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGTAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCATCACCTGCAg               <  2:1101615/150‑1 (MQ=255)
                                                                                                                                                aCTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTgctcttcg     <  2:784392/150‑1 (MQ=255)
                                                                                                                                                   gctgcGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTgctcttcgctc  >  1:399854/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTGCTCTTCGCTC  >  NC_002947/4744643‑4744939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑