Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,745,183 A→T 7.6% L13H (CTC→CAC)  PP_4199 ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,745,1830AT 7.6% 358.7 / 13.7 117L13H (CTC→CAC) PP_4199lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (47/60);  new base T (6/3);  total (54/63)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

TGGACTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTGT  >  NC_002947/4745034‑4745331
                                                                                                                                                     |                                                                                                                                                    
tGGACTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGa                                                                                                                                                      <  2:510775/150‑1 (MQ=255)
tGGACTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGa                                                                                                                                                      <  1:1391840/150‑1 (MQ=255)
  gACTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGc                                                                                                                                                    >  2:1218466/1‑150 (MQ=255)
          gcgGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAg                                                                                                                                            <  2:665046/150‑1 (MQ=255)
              gCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTGGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCa                                                                                                                                        >  2:1485661/1‑150 (MQ=255)
               ccAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGAGTTGCTCATTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGACATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACTGCCAGGGCaa                                                                                                                                       >  1:1375525/1‑150 (MQ=255)
               ccAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCa                                                                                                                                             <  1:1421557/144‑1 (MQ=255)
               ccAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCa                                                                                                                                             >  2:1421557/1‑144 (MQ=255)
               ccAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCaa                                                                                                                                       <  1:16974/150‑1 (MQ=255)
                cAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGCCTGGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAg                                                                                                                                      >  1:151289/1‑150 (MQ=255)
                 aGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACATACTCAGGGGCGCCGGCGGCCTTCGCCTGGTCCAGTGCCTGGTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAgg                                                                                                                                     <  1:770817/150‑1 (MQ=255)
                   cTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGAt                                                                                                                                   <  1:2067317/150‑1 (MQ=255)
                     tGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTa                                                                                                                                 <  2:446302/150‑1 (MQ=255)
                      gTCTTCAGCCTGTTTGAGCGTTTTGACCTGCTCAGTGGCGCCGACGGCCTTGGACTGGGCCGGTGCCTGTTCTGAAATGCGCCTCGCCTCCTCAGGGTCCGGATCAGTGGCGCGGCCAGCAACGCCGAGGCCGGGGAGGGCACGGACTAg                                                                                                                                >  2:417104/1‑150 (MQ=255)
                      gTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAg                                                                                                                                <  1:1121919/150‑1 (MQ=255)
                         ttCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATTCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTgg                                                                                                                             >  1:901782/1‑150 (MQ=255)
                         ttCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTgg                                                                                                                             >  2:1597308/1‑150 (MQ=255)
                         ttCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTgg                                                                                                                             >  1:478504/1‑150 (MQ=255)
                          tCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGt                                                                                                                            <  1:1597308/150‑1 (MQ=255)
                           cAGCCCGTTTCAGCGTTGCGCGCTGCTCATTGGCGCCGACGGCCTTGGCCTGGTCCTGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGtt                                                                                                                           <  1:417104/150‑1 (MQ=255)
                           cAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGtt                                                                                                                           <  2:1375525/150‑1 (MQ=255)
                             gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGAt                                                                                                                                   >  1:1433899/1‑140 (MQ=255)
                             gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGAt                                                                                                                                   <  2:1433899/140‑1 (MQ=255)
                             gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAg                                                                                                                                >  2:552130/1‑143 (MQ=255)
                             gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAg                                                                                                                                <  1:552130/143‑1 (MQ=255)
                             gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTgg                                                                                                                         <  1:1570624/150‑1 (MQ=255)
                             gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTgg                                                                                                                         <  2:480364/150‑1 (MQ=255)
                              ccAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGTGCTCGGcctgg                                                                                                                                           >  2:1011671/1‑128 (MQ=255)
                              ccAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGTGCTCGGCCTgg                                                                                                                                           <  1:1011671/131‑1 (MQ=255)
                              ccAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTggg                                                                                                                        >  1:1341995/1‑150 (MQ=255)
                              ccAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTggg                                                                                                                        >  1:416185/1‑150 (MQ=255)
                              ccAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTggg                                                                                                                        <  2:2375441/150‑1 (MQ=255)
                                    ccAGCGTTGCGCCCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCa                                                                                                                  <  2:364053/149‑1 (MQ=255)
                                     cAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCAc                                                                                                                 <  1:771150/150‑1 (MQ=255)
                                       gCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAg                                                                                                                                            <  1:1820355/121‑1 (MQ=255)
                                       gCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAg                                                                                                                                            >  2:1820355/1‑121 (MQ=255)
                                        cGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCa                                                                                                              >  1:940315/1‑150 (MQ=255)
                                           ttCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATc                                                                                                           <  1:475126/150‑1 (MQ=255)
                                               aCCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTcc                                                                                                       <  2:699104/150‑1 (MQ=255)
                                                ccTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCGGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCt                                                                                                      <  2:417842/150‑1 (MQ=255)
                                                 cTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTcc                                                                                                       <  2:1382668/148‑1 (MQ=255)
                                                  tGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACg                                                                                                                                                 <  2:306639/105‑1 (MQ=255)
                                                  tGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACg                                                                                                                                                 >  1:306639/1‑105 (MQ=255)
                                                   gCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAgt                                                                                                   <  1:706587/150‑1 (MQ=255)
                                                       aGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGttt                                                                                               <  1:2151540/150‑1 (MQ=255)
                                                       aGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGttt                                                                                               <  1:1390058/150‑1 (MQ=255)
                                                        gTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTg                                                                                              <  1:1176503/150‑1 (MQ=255)
                                                         tGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTgg                                                                                             <  2:1341995/150‑1 (MQ=255)
                                                          ggCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTggg                                                                                            <  2:2204348/150‑1 (MQ=255)
                                                          ggCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTggg                                                                                            <  1:1218466/150‑1 (MQ=255)
                                                                   ggCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGTATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTccgc                                                                                   >  1:2237484/1‑150 (MQ=255)
                                                                   ggCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTccgc                                                                                   <  2:1525162/150‑1 (MQ=255)
                                                                   ggCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTccgc                                                                                   <  2:901782/150‑1 (MQ=255)
                                                                     ccTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTgg                                                                                                                             >  2:713628/1‑106 (MQ=255)
                                                                     ccTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTgg                                                                                                                             <  1:713628/106‑1 (MQ=255)
                                                                     ccTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTccgccg                                                                                 >  1:89741/1‑150 (MQ=255)
                                                                      cTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAgg                                                                                                                                           <  1:1010388/91‑1 (MQ=255)
                                                                      cTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATCGGCGCAGCCAGCCAGGCCGTGCTCGGcc                                                                                                                                              >  1:825038/1‑88 (MQ=255)
                                                                      cTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATCGGCGCAGCCAGCCAGGCCGTGCTCGGcc                                                                                                                                              <  2:825038/88‑1 (MQ=255)
                                                                          gCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTAcc                                                                            >  1:1909358/1‑150 (MQ=255)
                                                                               gTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCAt                                                                       >  2:389969/1‑150 (MQ=255)
                                                                               gTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCAt                                                                       <  1:546192/150‑1 (MQ=255)
                                                                                   aGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTgc                                                                   <  2:478504/150‑1 (MQ=255)
                                                                                    gTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTgcg                                                                  >  1:558313/1‑150 (MQ=255)
                                                                                    gTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTgcg                                                                  >  2:1305920/1‑150 (MQ=255)
                                                                                       ccggTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGGGGTTGGGTTCTCACGCAATCTTCCTAGTGttt                                                                                               <  1:988435/115‑1 (MQ=255)
                                                                                         tGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTc                                                             <  1:1485661/150‑1 (MQ=255)
                                                                                           ttCGGGAATTCGCATCTGCTCTTCAGGGGCGGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCa                                                           <  2:508458/150‑1 (MQ=255)
                                                                                           ttCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGt                                                                                       <  1:2408962/122‑1 (MQ=255)
                                                                                           ttCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGt                                                                                       >  2:2408962/1‑122 (MQ=255)
                                                                                              ggAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGCGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAgcg                                                        >  2:1790430/1‑150 (MQ=255)
                                                                                              ggAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAgcg                                                        >  1:1420542/1‑150 (MQ=255)
                                                                                                      gcATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCt                                                                     <  1:1316490/129‑1 (MQ=255)
                                                                                                        aTCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACAttt                                              >  2:1060638/1‑150 (MQ=255)
                                                                                                             ctctTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTg                                         >  2:2161822/1‑150 (MQ=255)
                                                                                                             ctctTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTg                                         <  1:1305920/150‑1 (MQ=255)
                                                                                                              tctTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgc                                        >  2:2225516/1‑150 (MQ=255)
                                                                                                               ctTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgca                                       >  2:242968/1‑150 (MQ=255)
                                                                                                                ttCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgcag                                      >  2:1044125/1‑150 (MQ=255)
                                                                                                                ttCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgcag                                      >  2:9561/1‑150 (MQ=255)
                                                                                                                  cAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgcagca                                    <  2:151289/150‑1 (MQ=255)
                                                                                                                     gggCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTAc                                                                             <  2:1795057/106‑1 (MQ=255)
                                                                                                                     gggCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTAc                                                                             >  1:1795057/1‑106 (MQ=255)
                                                                                                                     gggCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAg                                 >  1:678484/1‑150 (MQ=255)
                                                                                                                        ccGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAg                                                                                                                                <  1:562101/52‑1 (MQ=255)
                                                                                                                        ccGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAg                                                                                                                                >  2:562101/1‑52 (MQ=255)
                                                                                                                         cGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTc                             <  2:416185/150‑1 (MQ=255)
                                                                                                                         cGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTc                             <  2:940315/150‑1 (MQ=255)
                                                                                                                            aTCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGa                          <  1:389969/150‑1 (MQ=255)
                                                                                                                            aTCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGa                          <  1:1060638/150‑1 (MQ=255)
                                                                                                                             tCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGAt                         <  2:89741/150‑1 (MQ=255)
                                                                                                                                ttGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCa                      >  1:1830396/1‑150 (MQ=255)
                                                                                                                                   gcgcAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGAGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCAccc                   >  1:157374/1‑149 (MQ=255)
                                                                                                                                         ttgccctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATc             >  1:222209/8‑150 (MQ=255)
                                                                                                                                          cagccagGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCg            <  1:1624932/150‑1 (MQ=255)
                                                                                                                                             cctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTgg                                                                                             >  1:1352790/4‑66 (MQ=255)
                                                                                                                                             cctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTgg                                                                                             <  2:1352790/63‑1 (MQ=255)
                                                                                                                                             cctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCa         >  2:1524676/4‑150 (MQ=255)
                                                                                                                                              cagGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGc          >  2:791707/1‑148 (MQ=255)
                                                                                                                                                    gAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTGt  <  1:2161822/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGGACTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTGT  >  NC_002947/4745034‑4745331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑