Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,745,186 A→T 7.8% V12E (GTG→GAG)  PP_4199 ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,745,1860AT 7.8% 363.5 / 16.3 116V12E (GTG→GAG) PP_4199lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (46/60);  new base T (6/3);  total (53/63)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

Only 100 of 119 total aligned reads displayed.

ACTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTGTCAGT  >  NC_002947/4745037‑4745335
                                                                                                                                                     |                                                                                                                                                     
aCTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCa                                                                                                                                                       >  2:475126/1‑150 (MQ=255)
  ttGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACg                                                                                                                                                     <  1:2265922/150‑1 (MQ=255)
       gcgGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAg                                                                                                                                                <  2:665046/150‑1 (MQ=255)
           gCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTGGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCa                                                                                                                                            >  2:1485661/1‑150 (MQ=255)
            ccAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGAGTTGCTCATTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGACATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACTGCCAGGGCaa                                                                                                                                           >  1:1375525/1‑150 (MQ=255)
            ccAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCa                                                                                                                                                 <  1:1421557/144‑1 (MQ=255)
            ccAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCa                                                                                                                                                 >  2:1421557/1‑144 (MQ=255)
             cAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGCCTGGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAg                                                                                                                                          >  1:151289/1‑150 (MQ=255)
              aGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACATACTCAGGGGCGCCGGCGGCCTTCGCCTGGTCCAGTGCCTGGTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAgg                                                                                                                                         <  1:770817/150‑1 (MQ=255)
               gCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGa                                                                                                                                        >  1:446302/1‑150 (MQ=255)
                   gTCTTCAGCCTGTTTGAGCGTTTTGACCTGCTCAGTGGCGCCGACGGCCTTGGACTGGGCCGGTGCCTGTTCTGAAATGCGCCTCGCCTCCTCAGGGTCCGGATCAGTGGCGCGGCCAGCAACGCCGAGGCCGGGGAGGGCACGGACTAg                                                                                                                                    >  2:417104/1‑150 (MQ=255)
                   gTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAg                                                                                                                                    <  1:1121919/150‑1 (MQ=255)
                      ttCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATTCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTgg                                                                                                                                 >  1:901782/1‑150 (MQ=255)
                      ttCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTgg                                                                                                                                 >  1:478504/1‑150 (MQ=255)
                       tCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGt                                                                                                                                <  1:1597308/150‑1 (MQ=255)
                        cAGCCCGTTTCAGCGTTGCGCGCTGCTCATTGGCGCCGACGGCCTTGGCCTGGTCCTGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGtt                                                                                                                               <  1:417104/150‑1 (MQ=255)
                        cAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGtt                                                                                                                               <  2:1375525/150‑1 (MQ=255)
                          gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGAt                                                                                                                                       >  1:1433899/1‑140 (MQ=255)
                          gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAg                                                                                                                                    >  2:552130/1‑143 (MQ=255)
                          gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAg                                                                                                                                    <  1:552130/143‑1 (MQ=255)
                          gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTgg                                                                                                                             <  2:480364/150‑1 (MQ=255)
                          gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTgg                                                                                                                             <  1:1570624/150‑1 (MQ=255)
                          gCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTgg                                                                                                                             >  2:1650084/1‑150 (MQ=255)
                           ccAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGTGCTCGGcctgg                                                                                                                                               >  2:1011671/1‑128 (MQ=255)
                           ccAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGTGCTCGGCCTgg                                                                                                                                               <  1:1011671/131‑1 (MQ=255)
                           ccAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTggg                                                                                                                            <  2:2375441/150‑1 (MQ=255)
                           ccAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTggg                                                                                                                            >  1:1341995/1‑150 (MQ=255)
                           ccAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTggg                                                                                                                            >  1:416185/1‑150 (MQ=255)
                             aGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGtt                                                                                                                          <  1:1650084/150‑1 (MQ=255)
                                 tCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCa                                                                                                                      <  1:2053101/150‑1 (MQ=255)
                                 ccAGCGTTGCGCCCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCa                                                                                                                      <  2:364053/149‑1 (MQ=255)
                                  cAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCAc                                                                                                                     <  1:771150/150‑1 (MQ=255)
                                    gCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAg                                                                                                                                                >  2:1820355/1‑121 (MQ=255)
                                    gCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAg                                                                                                                                                <  1:1820355/121‑1 (MQ=255)
                                     cGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCa                                                                                                                  >  1:940315/1‑150 (MQ=255)
                                            aCCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTcc                                                                                                           <  2:699104/150‑1 (MQ=255)
                                             ccTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCGGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCt                                                                                                          <  2:417842/150‑1 (MQ=255)
                                              cTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTcc                                                                                                           <  2:1382668/148‑1 (MQ=255)
                                               tGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACg                                                                                                                                                     >  1:306639/1‑105 (MQ=255)
                                                gCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAgt                                                                                                       <  1:706587/150‑1 (MQ=255)
                                                    aGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGttt                                                                                                   <  1:1390058/150‑1 (MQ=255)
                                                    aGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGttt                                                                                                   <  1:2151540/150‑1 (MQ=255)
                                                     gTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTg                                                                                                  <  1:1176503/150‑1 (MQ=255)
                                                       ggCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTggg                                                                                                <  1:1218466/150‑1 (MQ=255)
                                                                ggCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGTATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTccgc                                                                                       >  1:2237484/1‑150 (MQ=255)
                                                                ggCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTccgc                                                                                       <  2:901782/150‑1 (MQ=255)
                                                                ggCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTccgc                                                                                       <  2:1525162/150‑1 (MQ=255)
                                                                  ccTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTgg                                                                                                                                 <  1:713628/106‑1 (MQ=255)
                                                                  ccTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTgg                                                                                                                                 >  2:713628/1‑106 (MQ=255)
                                                                  ccTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTccgccg                                                                                     >  1:89741/1‑150 (MQ=255)
                                                                   cTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAgg                                                                                                                                               <  1:1010388/91‑1 (MQ=255)
                                                                   cTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATCGGCGCAGCCAGCCAGGCCGTGCTCGGcc                                                                                                                                                  >  1:825038/1‑88 (MQ=255)
                                                                   cTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATCGGCGCAGCCAGCCAGGCCGTGCTCGGcc                                                                                                                                                  <  2:825038/88‑1 (MQ=255)
                                                                       gCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTAcc                                                                                >  1:1909358/1‑150 (MQ=255)
                                                                            gTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCAt                                                                           >  2:389969/1‑150 (MQ=255)
                                                                            gTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCAt                                                                           <  1:546192/150‑1 (MQ=255)
                                                                                aGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTgc                                                                       <  2:478504/150‑1 (MQ=255)
                                                                                aGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTgc                                                                       <  1:568847/150‑1 (MQ=255)
                                                                                 gTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTgcg                                                                      >  2:1305920/1‑150 (MQ=255)
                                                                                 gTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTgcg                                                                      >  1:558313/1‑150 (MQ=255)
                                                                                    ccggTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGGGGTTGGGTTCTCACGCAATCTTCCTAGTGttt                                                                                                   <  1:988435/115‑1 (MQ=255)
                                                                                        ttCGGGAATTCGCATCTGCTCTTCAGGGGCGGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCa                                                               <  2:508458/150‑1 (MQ=255)
                                                                                        ttCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGt                                                                                           >  2:2408962/1‑122 (MQ=255)
                                                                                           ggAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGCGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAgcg                                                            >  2:1790430/1‑150 (MQ=255)
                                                                                           ggAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAgcg                                                            >  1:1420542/1‑150 (MQ=255)
                                                                                                   gcATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCt                                                                         >  2:1316490/1‑129 (MQ=255)
                                                                                                   gcATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCt                                                                         <  1:1316490/129‑1 (MQ=255)
                                                                                                     aTCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACAttt                                                  >  2:1060638/1‑150 (MQ=255)
                                                                                                          ctctTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTg                                             <  1:1305920/150‑1 (MQ=255)
                                                                                                          ctctTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTg                                             >  2:2161822/1‑150 (MQ=255)
                                                                                                           tctTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgc                                            >  2:2225516/1‑150 (MQ=255)
                                                                                                            ctTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgca                                           >  2:242968/1‑150 (MQ=255)
                                                                                                             ttCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgcag                                          >  2:9561/1‑150 (MQ=255)
                                                                                                             ttCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgcag                                          >  2:1044125/1‑150 (MQ=255)
                                                                                                               cAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgcagca                                        <  2:151289/150‑1 (MQ=255)
                                                                                                                  gggCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTAc                                                                                 <  2:1795057/106‑1 (MQ=255)
                                                                                                                  gggCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTAc                                                                                 >  1:1795057/1‑106 (MQ=255)
                                                                                                                  gggCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAg                                     >  1:678484/1‑150 (MQ=255)
                                                                                                                     ccGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAg                                                                                                                                    >  2:562101/1‑52 (MQ=255)
                                                                                                                     ccGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAg                                                                                                                                    <  1:562101/52‑1 (MQ=255)
                                                                                                                      cGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTc                                 <  2:940315/150‑1 (MQ=255)
                                                                                                                      cGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTc                                 <  2:416185/150‑1 (MQ=255)
                                                                                                                         aTCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGa                              <  1:389969/150‑1 (MQ=255)
                                                                                                                         aTCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGa                              <  1:1060638/150‑1 (MQ=255)
                                                                                                                          tCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGAt                             <  2:89741/150‑1 (MQ=255)
                                                                                                                             ttGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCa                          >  1:1830396/1‑150 (MQ=255)
                                                                                                                                gcgcAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGAGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCAccc                       >  1:157374/1‑149 (MQ=255)
                                                                                                                                     gccagccaGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGAt                  >  2:2223390/1‑150 (MQ=255)
                                                                                                                                      ttgccctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATc                 >  2:333245/8‑150 (MQ=255)
                                                                                                                                      ttgccctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATc                 >  1:222209/8‑150 (MQ=255)
                                                                                                                                       cagccagGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCg                <  1:1624932/150‑1 (MQ=255)
                                                                                                                                          cctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTgg                                                                                                 <  2:1352790/63‑1 (MQ=255)
                                                                                                                                          cctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTgg                                                                                                 >  1:1352790/4‑66 (MQ=255)
                                                                                                                                          cctggCCGTGCTCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCa             >  2:1524676/4‑150 (MQ=255)
                                                                                                                                           cagGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGc              >  2:791707/1‑148 (MQ=255)
                                                                                                                                                cGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgc                                            <  1:1448799/113‑1 (MQ=255)
                                                                                                                                                cGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTgc                                            >  2:1448799/1‑113 (MQ=255)
                                                                                                                                                 gAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTGt      <  1:2161822/150‑1 (MQ=255)
                                                                                                                                                   gCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTGTCa    >  2:1365488/1‑150 (MQ=255)
                                                                                                                                                     aCGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGAACCAGATATCGGCAATTCTGTCAGt  <  2:1420542/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTTGGCGCGGGCCAGCTTGTCTTCAGCCAGTTTCAGCGTTTCGACCTGCTCAGTGGCGCCGACGGCCTTGGCCTGGTCCAGTGCCTGTTCGGAAATGCGCATCTGCTCTTCAGGGGCCGGATCATTGGCGCAGCCAGCCAGGCCGAGCACGGCCAGGGCAAGGATTAGTGGTTGGGTTCTCACGCAATCTTCCTAGTGTTTGGGGGCGTCCGCCGGTACCTGCATCTGCGCTTTCCAGCGCTCGACATTTCGTTGCAGCACAGCTTCGGATGCACCAGAGATCGGCAATTCTGTCAGT  >  NC_002947/4745037‑4745335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑