Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,760,265 C→A 5.7% D94E (GAC→GAA)  pvdM → dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,760,2650CA 5.7% 390.5 / 5.9 119D94E (GAC→GAA) pvdMdipeptidase
Reads supporting (aligned to +/- strand):  ref base C (61/51);  new base A (3/4);  total (64/55)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 124 total aligned reads displayed.

TTCGACAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGAAGTGCGCTACAAC  >  NC_002947/4760116‑4760412
                                                                                                                                                     |                                                                                                                                                   
ttCGACAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGAc                                                                                                                                                     >  2:1839519/1‑150 (MQ=255)
ttCGACAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCATTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGAGACGAGGGCCGCGAAGCGGACAAGGATGGCCCGGGCCAGATCGAc                                                                                                                                                     >  1:1121228/1‑150 (MQ=255)
   gACAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTg                                                                                                                                                  >  1:1765862/1‑150 (MQ=255)
   gACAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTg                                                                                                                                                  >  1:1442599/1‑150 (MQ=255)
    aCAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTgg                                                                                                                                                 <  2:2227708/150‑1 (MQ=255)
     cAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGc                                                                                                                                                >  2:2294544/1‑150 (MQ=255)
         gACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCaaa                                                                                                                                            <  2:1765862/150‑1 (MQ=255)
         gACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGGTCGAACTGGcccgg                                                                                                                                            <  2:330927/150‑4 (MQ=255)
          aCGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCCGCGAAGGCCGCGAAGCGGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAg                                                                                                                                           <  1:1378655/150‑1 (MQ=255)
          aCGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAg                                                                                                                                           <  1:2130441/150‑1 (MQ=255)
          aCGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGGTCGAACTGGcccggg                                                                                                                                           <  2:2023343/150‑5 (MQ=255)
              cTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCcgc                                                                                                                                       <  1:1387556/150‑1 (MQ=255)
              cTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCcgc                                                                                                                                       >  1:2061025/1‑150 (MQ=255)
                 ccccATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCcgcgcg                                                                                                                                    >  1:365641/1‑150 (MQ=255)
                 ccccATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGCCACGGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCcgcgcg                                                                                                                                    >  2:74768/1‑150 (MQ=255)
                  cccATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCcgcgcgc                                                                                                                                   >  2:452760/1‑150 (MQ=255)
                     aTAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCggg                                                                                                                                <  2:632160/150‑1 (MQ=255)
                     aTAGAGGTCGTGCGCCAGGCAAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCggg                                                                                                                                <  2:1161922/150‑1 (MQ=255)
                       agagGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCg                                                                                                                              >  1:864669/1‑150 (MQ=255)
                           gTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTg                                                                                                                          >  1:1283655/1‑150 (MQ=255)
                                   ccAGGCCAATGATCCGAAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGgcgc                                                                                                                  >  2:1792656/1‑150 (MQ=255)
                                      ggCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCcgc                                                                                                                                       >  2:1660707/1‑126 (MQ=255)
                                       gCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGcc                                                                                                              >  2:733401/1‑150 (MQ=255)
                                       cccAATGATCTGCAAGCGCGGATCATTTCGTTCGACCGCCATCTCCCCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGcc                                                                                                              <  2:1331784/149‑1 (MQ=255)
                                           aTGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGa                                                                                                          <  2:1185327/150‑1 (MQ=255)
                                           aTGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGa                                                                                                          >  2:1564024/1‑150 (MQ=255)
                                           aTGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGa                                                                                                          >  2:1223894/1‑150 (MQ=255)
                                                cTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCATAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTc                                                                                                     <  1:2294544/150‑1 (MQ=255)
                                                  gCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCtt                                                                                                   <  2:2061025/150‑1 (MQ=255)
                                                  gCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCGGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTAGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCtt                                                                                                   >  2:2353616/1‑150 (MQ=255)
                                                   cAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTc                                                                                                  <  2:1442599/150‑1 (MQ=255)
                                                   cAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTc                                                                                                  >  2:378656/1‑150 (MQ=255)
                                                        gCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTg                                                                                             <  1:381544/150‑1 (MQ=255)
                                                          ggATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGc                                                                                           >  2:1880869/1‑150 (MQ=255)
                                                            aTCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGccc                                                                                         <  1:1564024/150‑1 (MQ=255)
                                                               aTTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAg                                                                                      <  1:841676/150‑1 (MQ=255)
                                                                tttCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGAc                                                                                                                                                     >  2:1560312/1‑86 (MQ=255)
                                                                tttCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGAc                                                                                                                                                     <  1:1560312/86‑1 (MQ=255)
                                                                tttCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGt                                                                                     >  2:1320057/1‑150 (MQ=255)
                                                                   cgttcgACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCt                                                                                  <  1:1839519/150‑1 (MQ=255)
                                                                    gttcgACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCgg                                                                                                                                 >  1:1878368/1‑102 (MQ=255)
                                                                    gttcgACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCgg                                                                                                                                 <  2:1878368/102‑1 (MQ=255)
                                                                    gttcgACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTg                                                                                 >  2:56948/1‑150 (MQ=255)
                                                                    gttcgACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGAATGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTg                                                                                 >  1:344163/1‑150 (MQ=255)
                                                                     ttcgACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTgg                                                                                >  1:664439/1‑150 (MQ=255)
                                                                       cgACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTg                                                                                             <  1:1851203/135‑1 (MQ=255)
                                                                       cgACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTg                                                                                             >  2:1851203/1‑135 (MQ=255)
                                                                           aGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAg                                                                                      >  2:1829292/1‑138 (MQ=255)
                                                                           aGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAg                                                                                      <  1:1829292/138‑1 (MQ=255)
                                                                           aGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGc                                                                          >  1:669950/1‑150 (MQ=255)
                                                                             ccATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGa                                                                                                          >  1:118114/1‑116 (MQ=255)
                                                                             ccATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCCCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGa                                                                        <  1:739223/150‑1 (MQ=255)
                                                                              cATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGAc                                                                       >  1:2306631/1‑150 (MQ=255)
                                                                               aTCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCt                                                                                  <  1:2096998/138‑1 (MQ=255)
                                                                               aTCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACa                                                                      >  1:1538778/1‑150 (MQ=255)
                                                                                tctcACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGc                                                                          >  2:1219546/1‑145 (MQ=255)
                                                                                tctcACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGc                                                                          <  1:1219546/145‑1 (MQ=255)
                                                                                tctcACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACaa                                                                     <  1:1223894/150‑1 (MQ=255)
                                                                                  tcACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACg                                                                   <  2:365641/150‑1 (MQ=255)
                                                                                    aCCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGc                                                                 >  1:915230/1‑150 (MQ=255)
                                                                                    aCCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGc                                                                 >  1:914950/1‑150 (MQ=255)
                                                                                      ccTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGccc                                                               >  1:2307655/1‑150 (MQ=255)
                                                                                        tGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGc                                                             <  2:914950/150‑1 (MQ=255)
                                                                                             cTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGcaccac                                                        <  2:664439/150‑1 (MQ=255)
                                                                                             cTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGcaccac                                                        <  1:733401/150‑1 (MQ=255)
                                                                                             cTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGcaccac                                                        <  2:2180997/150‑1 (MQ=255)
                                                                                                   ttCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCAccaccccacc                                                  >  1:1798667/1‑150 (MQ=255)
                                                                                                       gCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACg                                              >  1:1028065/1‑150 (MQ=255)
                                                                                                         aCCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGt                                            >  2:609137/1‑150 (MQ=255)
                                                                                                           cGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCt                                                                                  <  2:707145/110‑1 (MQ=255)
                                                                                                           cGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGc                                                                          >  2:20539/1‑118 (MQ=255)
                                                                                                              aGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTtcgt                                       <  2:864669/150‑1 (MQ=255)
                                                                                                                  cgcgAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGAt                                   <  2:1919621/150‑1 (MQ=255)
                                                                                                                   gcgAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGCCCTGGACCGGAGAGAACGGGGCGGGCCACCCCACCGACGGTTTCGTCGATg                                  >  2:1468517/1‑150 (MQ=255)
                                                                                                                     gAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGcc                                >  1:1943675/1‑150 (MQ=255)
                                                                                                                         cTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGc                            <  2:1112995/150‑1 (MQ=255)
                                                                                                                           gACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGCCGACCACGGCCCGCACCACCCCACCGCCGGTTTCGTCGATGCCGGGCgg                          >  2:2340387/1‑149 (MQ=255)
                                                                                                                            aCAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGAc                         >  2:1176122/1‑150 (MQ=255)
                                                                                                                            aCAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGCTCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGAc                         >  1:559838/1‑150 (MQ=255)
                                                                                                                              aaGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCg                                                 >  2:2325706/1‑124 (MQ=255)
                                                                                                                              aaGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCg                                                 <  1:2325706/124‑1 (MQ=255)
                                                                                                                              aaGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACAt                       >  2:1134707/1‑150 (MQ=255)
                                                                                                                                ggATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGc                                                                 <  1:2142290/106‑1 (MQ=255)
                                                                                                                                ggATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGc                                                                 >  2:2142290/1‑106 (MQ=255)
                                                                                                                                ggATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGc                     >  1:970525/1‑150 (MQ=255)
                                                                                                                                 gATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAATCATGGACCGGCGACAACGACCGGCACCACCCCACCGACGGTTTCGGCGTTGCCGGGCGACATGcc                    >  2:793202/1‑150 (MQ=255)
                                                                                                                                   tGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCa                  <  1:828112/150‑1 (MQ=255)
                                                                                                                                      ctttggCCAGGTCGAACTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGGCCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGa               >  1:1987173/5‑150 (MQ=255)
                                                                                                                                      cccGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGa               >  2:87025/1‑150 (MQ=255)
                                                                                                                                       tttggCCAGGTCGCACTGGCGAAAGCGGGGCGCGGGCGACTGTCCGGCGCTGCCCTGGCCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGaa              <  2:1987173/147‑1 (MQ=255)
                                                                                                                                       ccGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGaa              <  1:609137/150‑1 (MQ=255)
                                                                                                                                       ccGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGaa              >  2:252661/1‑150 (MQ=255)
                                                                                                                                       ccGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGaa              >  2:854181/1‑150 (MQ=255)
                                                                                                                                            ccAGGTCGAACTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGAcc                                                                                                        <  1:2188838/55‑1 (MQ=255)
                                                                                                                                            ccAGGTCGAACTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGAcc                                                                                                        >  2:2188838/1‑55 (MQ=255)
                                                                                                                                             cAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGAAGTgcgc        <  1:378656/150‑1 (MQ=255)
                                                                                                                                             cAGGTCGAACTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGcc                                                                                                              >  2:1492861/1‑48 (MQ=255)
                                                                                                                                                   gACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGCAGTGCGCGACAAc  <  2:1354648/150‑1 (MQ=255)
                                                                                                                                                    aCCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCTGGGCGACAt                       <  1:1656985/128‑1 (MQ=255)
                                                                                                                                                    aCCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCTGGGCGACAt                       >  2:1656985/1‑128 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTCGACAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGGCACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGAAGTGCGCTACAAC  >  NC_002947/4760116‑4760412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑