Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 384,786 C→G 5.8% R421G (CGC→GGC)  PP_0320 → methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947384,7860CG 5.8% 419.1 / 11.3 131R421G (CGC→GGC) PP_0320methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base C (68/55);  new base G (5/3);  total (73/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

AGTACCTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCCGAC  >  NC_002947/384639‑384935
                                                                                                                                                   |                                                                                                                                                     
     cTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCGCTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGc                                                                                                                                                >  1:2110875/1‑150 (MQ=255)
        cGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCgg                                                                                                                                             >  2:801740/1‑150 (MQ=255)
               cTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCg                                                                                                                                      >  1:869959/1‑150 (MQ=255)
                tGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCgg                                                                                                                                     >  1:142781/1‑150 (MQ=255)
                        gCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCCCGCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCg                                                                                                                             >  1:1159493/1‑150 (MQ=255)
                         ccGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGt                                                                                                                            >  2:170563/1‑150 (MQ=255)
                          cGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTc                                                                                                                           <  2:2122986/150‑1 (MQ=255)
                          cGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTc                                                                                                                           <  1:2391302/150‑1 (MQ=255)
                               aGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGGCATCGCCGGCATTGCGGGCGATgtcct                                                                                                                                    <  1:1145400/136‑5 (MQ=255)
                               aGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGGCATCGCCGGCATTGCGGGCGATgtcct                                                                                                                                    >  2:1145400/1‑132 (MQ=255)
                                      aaCCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGccc                                                                                                                                                       <  1:1566996/110‑1 (MQ=255)
                                       aCCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacga                                                                                                              <  1:170563/150‑1 (MQ=255)
                                         ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgaga                                                                                                            >  1:1042904/1‑150 (MQ=255)
                                         ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgaga                                                                                                            >  2:946954/1‑150 (MQ=255)
                                         ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgaga                                                                                                            >  2:708718/1‑150 (MQ=255)
                                         ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgaga                                                                                                            <  1:597404/150‑1 (MQ=255)
                                         ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgaga                                                                                                            >  1:1535278/1‑150 (MQ=255)
                                                gtggACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCg                                                                                                     >  2:1097937/1‑150 (MQ=255)
                                                gtggACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCg                                                                                                     >  2:2017431/1‑150 (MQ=255)
                                                 tggACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGt                                                                                                    >  2:234593/1‑150 (MQ=255)
                                                   gACAACACCGCCGAGCGCTCCGAGCGACAGCAGGAGATGAAAGCAATCTTCTCCACTGCGGTAGTCGAGATTGGCCTGGCCATACAGGACATCGCCCGCAATGCCGGCGATGCGGCGCAGGCGTCGCAGGCGGCACGAGACGAGGCGTTg                                                                                                  >  2:276841/1‑150 (MQ=255)
                                                     caacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGcg                                                                                                <  2:1655151/150‑2 (MQ=255)
                                                      aacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACTGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAg                                                                                               >  2:1389936/1‑150 (MQ=255)
                                                      aacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAg                                                                                               >  1:313854/1‑150 (MQ=255)
                                                      aacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAg                                                                                               >  2:1464081/1‑150 (MQ=255)
                                                         acCGCTGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGcc                                                                                            >  2:401948/1‑150 (MQ=255)
                                                           cgccgAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCg                                                                                                                          >  1:1360444/1‑118 (MQ=255)
                                                           cgccgAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGACGCCACTGCTGTACACGAGATGAGCCTGACCGTGCAAGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGCCACGCGTCGAGGCGTTGCAGGcgcg                                                                                          >  1:152972/1‑150 (MQ=255)
                                                                aGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCCGTCGGCACGAGACGAGTCGTTGCAGGCCCGCGatg                                                                                     <  2:1352514/150‑3 (MQ=255)
                                                                 gcgcTCCGGGCGCCAGCAGGAGATGACCGAAATGGTTGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGGCGAGGCGTTGCAGGCCCGCGAggt                                                                                    >  2:197825/1‑150 (MQ=255)
                                                                 gcgcTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggt                                                                                    <  2:1545973/150‑1 (MQ=255)
                                                                  cgcTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggtg                                                                                   <  1:2034923/150‑1 (MQ=255)
                                                                    cTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggtggt                                                                                 <  2:2072646/150‑1 (MQ=255)
                                                                     tCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGgtggtg                                                                                >  2:1581813/1‑150 (MQ=255)
                                                                       cGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCg                                                                                                                                              <  2:1534931/86‑1 (MQ=255)
                                                                       cGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCa                                                                              >  1:2112535/1‑150 (MQ=255)
                                                                        gggCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCaa                                                                             <  2:313854/150‑1 (MQ=255)
                                                                        gggCGCCAGCAAGAGAAGACCCAAATGCGCGGCACTGCTGTACACGAGATGGGCATGACCGTGCAGGACATCGCCCGCAATGCCGGTGATACGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCTCGCGAGGTGTTGCaa                                                                             <  2:1932946/150‑1 (MQ=255)
                                                                              cagcagGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATcc                                                                       >  1:2227121/1‑150 (MQ=255)
                                                                                  agGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTc                                                                   <  2:496619/150‑1 (MQ=255)
                                                                                    gagaTGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACCTCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGt                                                                 >  2:1443826/1‑150 (MQ=255)
                                                                                    gagaTGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGt                                                                 >  2:1034179/1‑150 (MQ=255)
                                                                                     agaTGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTg                                                                <  1:1237508/150‑1 (MQ=255)
                                                                                          aCCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAg                                                                                               <  1:1867061/114‑1 (MQ=255)
                                                                                            cGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGCTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGa                                                         >  1:1845456/1‑150 (MQ=255)
                                                                                             gAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGCTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAg                                                        <  2:1845456/150‑1 (MQ=255)
                                                                                               aaTGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggtgg                                                                                  >  2:246040/1‑122 (MQ=255)
                                                                                                  ggTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCAt                                                   <  2:1072185/150‑1 (MQ=255)
                                                                                                   gTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGAt                                                                         <  1:116385/127‑1 (MQ=255)
                                                                                                   gTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGAt                                                                         >  2:116385/1‑127 (MQ=255)
                                                                                                    tCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTgg                                                               <  1:318670/136‑1 (MQ=255)
                                                                                                    tCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTgg                                                               >  2:318670/1‑136 (MQ=255)
                                                                                                       gatctgctgTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAAATGCTGCGATGCTGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCgg                                              <  1:1353465/147‑1 (MQ=255)
                                                                                                       ccACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCa                                                                                                                 <  1:806433/83‑1 (MQ=255)
                                                                                                       ccACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCa                                                                                                                 >  2:806433/1‑83 (MQ=255)
                                                                                                       ccACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCgg                                              <  1:946954/150‑1 (MQ=255)
                                                                                                        cACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCac                                                                                                                <  2:2399550/83‑1 (MQ=255)
                                                                                                        cACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCac                                                                                                                >  1:2399550/1‑83 (MQ=255)
                                                                                                        cACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCggg                                             >  1:197348/1‑150 (MQ=255)
                                                                                                         aCTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGGGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGAATCGAGGACATGTCGGGc                                            <  1:83127/150‑1 (MQ=255)
                                                                                                         aCTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGc                                            <  2:197348/150‑1 (MQ=255)
                                                                                                         aCTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGc                                            <  2:1159493/150‑1 (MQ=255)
                                                                                                           tgctgTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTg                                                                >  1:1037137/1‑128 (MQ=255)
                                                                                                           tgctgTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTg                                                                <  2:1037137/128‑1 (MQ=255)
                                                                                                             ctgTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGAAGAGGCGTTGCAGGCCCGAGAGGAGCTGCAACGATCCATTCGTGGCATCGAGGGCAGGTCGGgagatc                                        >  2:1353465/1‑148 (MQ=255)
                                                                                                                tACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCg                                               >  2:459041/1‑140 (MQ=255)
                                                                                                                tACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCg                                     <  1:234593/150‑1 (MQ=255)
                                                                                                                  caagagaTGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGc                                   <  1:855448/147‑1 (MQ=255)
                                                                                                                   acGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCg                                     <  1:2014632/147‑1 (MQ=255)
                                                                                                                   acGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCg                                     >  2:2014632/1‑147 (MQ=255)
                                                                                                                   acGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCa                                  >  1:73971/1‑150 (MQ=255)
                                                                                                                    cGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCaa                                 <  1:2317200/150‑1 (MQ=255)
                                                                                                                        aTGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAggcg                             >  2:1326084/1‑150 (MQ=255)
                                                                                                                             ccTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgagacga                                                                                                         >  2:2159615/1‑69 (MQ=255)
                                                                                                                             ccTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGa                        >  1:1959132/1‑150 (MQ=255)
                                                                                                                                 aCCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCg                    >  1:596914/1‑150 (MQ=255)
                                                                                                                                  ccGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCgg                   >  1:1935722/1‑150 (MQ=255)
                                                                                                                                       cAGGACATCGCCCGCAATGCCGGCGATGCGGCCCCCCGGTGGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCCAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagc              <  1:1330625/150‑1 (MQ=255)
                                                                                                                                        aGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGAt                       >  1:2129681/1‑140 (MQ=255)
                                                                                                                                        aGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGAt                       <  2:2129681/140‑1 (MQ=255)
                                                                                                                                        aGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagcc             >  2:1257020/1‑150 (MQ=255)
                                                                                                                                        aGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagcc             >  1:1072824/1‑150 (MQ=255)
                                                                                                                                         ccgcATCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagcca            >  2:1919872/4‑150 (MQ=255)
                                                                                                                                           gcATCGCCGGCATTGCGGGCGATGTGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTg                                                                <  1:1266502/95‑1 (MQ=38)
                                                                                                                                           gcATCGCCGGCATTGCGGGCGATGTGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTg                                                                >  2:1266502/2‑96 (MQ=38)
                                                                                                                                           gcATCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagccagc          >  1:1692349/2‑150 (MQ=255)
                                                                                                                                           gcATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagccagc          >  2:380893/2‑150 (MQ=255)
                                                                                                                                           aCATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagccagc          <  2:1846048/150‑1 (MQ=255)
                                                                                                                                           aCATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagccagc          <  2:1935722/150‑1 (MQ=255)
                                                                                                                                             aTCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTca                <  2:2344829/142‑1 (MQ=255)
                                                                                                                                             aTCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGc                     >  1:1620957/1‑137 (MQ=255)
                                                                                                                                             aTCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGc                     <  2:1620957/137‑1 (MQ=255)
                                                                                                                                              tCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATg                      >  2:1788201/1‑135 (MQ=255)
                                                                                                                                               tgaccGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGGTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGTGCATGTCGGGCGAGATTGGCAAGGCGTCCGATTCGGTGAACGACCTGGc      >  2:509507/4‑150 (MQ=255)
                                                                                                                                               cGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATc                                                                        >  2:1833740/1‑84 (MQ=255)
                                                                                                                                               cGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATc                                                                        <  1:1833740/84‑1 (MQ=255)
                                                                                                                                                  ccGCATTGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCcga   <  2:869959/150‑1 (MQ=255)
                                                                                                                                                  ccGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCcga   >  2:1883582/1‑150 (MQ=255)
                                                                                                                                                   cGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGc                                                                                                                  >  2:1876645/1‑38 (MQ=255)
                                                                                                                                                   cGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCcgac  >  1:832942/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGTACCTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCCGAC  >  NC_002947/384639‑384935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑