Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 384,790 A→T 6.4% N422I (AAT→ATT)  PP_0320 → methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947384,7900AT 6.4% 438.9 / 12.6 136N422I (AAT→ATT) PP_0320methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base A (71/56);  new base T (5/4);  total (76/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

CTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCCGACGAA  >  NC_002947/384644‑384938
                                                                                                                                                  |                                                                                                                                                    
cTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCGCTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGc                                                                                                                                                   >  1:2110875/1‑150 (MQ=255)
   cGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCgg                                                                                                                                                >  2:801740/1‑150 (MQ=255)
   cGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCgg                                                                                                                                                <  1:2097593/150‑1 (MQ=255)
           tGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCgg                                                                                                                                        >  1:142781/1‑150 (MQ=255)
                   gCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCCCGCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCg                                                                                                                                >  1:1159493/1‑150 (MQ=255)
                    ccGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGt                                                                                                                               >  2:170563/1‑150 (MQ=255)
                     cGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTc                                                                                                                              <  2:2122986/150‑1 (MQ=255)
                     cGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTc                                                                                                                              <  1:801740/150‑1 (MQ=255)
                     cGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTc                                                                                                                              <  1:2391302/150‑1 (MQ=255)
                          aGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGGCATCGCCGGCATTGCGGGCGATgtcct                                                                                                                                       <  1:1145400/136‑5 (MQ=255)
                          aGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGGCATCGCCGGCATTGCGGGCGATgtcct                                                                                                                                       >  2:1145400/1‑132 (MQ=255)
                                tAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCac                                                                                                                   >  1:92070/1‑150 (MQ=255)
                                  aCCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacga                                                                                                                 <  1:170563/150‑1 (MQ=255)
                                    ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgaga                                                                                                               >  2:946954/1‑150 (MQ=255)
                                    ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgaga                                                                                                               >  2:708718/1‑150 (MQ=255)
                                    ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgaga                                                                                                               <  1:597404/150‑1 (MQ=255)
                                    ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgaga                                                                                                               >  1:1042904/1‑150 (MQ=255)
                                    ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgaga                                                                                                               >  1:1535278/1‑150 (MQ=255)
                                           gtggACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCg                                                                                                        >  2:2017431/1‑150 (MQ=255)
                                              gACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTg                                                                                                     >  2:130374/1‑150 (MQ=255)
                                              gACAACACCGCCGAGCGCTCCGAGCGACAGCAGGAGATGAAAGCAATCTTCTCCACTGCGGTAGTCGAGATTGGCCTGGCCATACAGGACATCGCCCGCAATGCCGGCGATGCGGCGCAGGCGTCGCAGGCGGCACGAGACGAGGCGTTg                                                                                                     >  2:276841/1‑150 (MQ=255)
                                               acaacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGc                                                                                                    <  2:1917367/150‑1 (MQ=255)
                                                caacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGcg                                                                                                   <  2:1655151/150‑2 (MQ=255)
                                                 aacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACTGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAg                                                                                                  >  2:1389936/1‑150 (MQ=255)
                                                 aacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAg                                                                                                  >  1:313854/1‑150 (MQ=255)
                                                 aacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAg                                                                                                  >  2:1464081/1‑150 (MQ=255)
                                                    acCGCTGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGcc                                                                                               >  2:401948/1‑150 (MQ=255)
                                                      cgccgAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCg                                                                                                                             <  2:1360444/118‑1 (MQ=255)
                                                           aGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCCGTCGGCACGAGACGAGTCGTTGCAGGCCCGCGatg                                                                                        <  2:1352514/150‑3 (MQ=255)
                                                                tCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGgtggtg                                                                                   >  2:1581813/1‑150 (MQ=255)
                                                                  cGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCg                                                                                                                                                 <  2:1534931/86‑1 (MQ=255)
                                                                  cGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCg                                                                                                                                                 >  1:1534931/1‑86 (MQ=255)
                                                                  cGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCa                                                                                 >  1:2112535/1‑150 (MQ=255)
                                                                   gggCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCaa                                                                                <  2:313854/150‑1 (MQ=255)
                                                                   gggCGCCAGCAAGAGAAGACCCAAATGCGCGGCACTGCTGTACACGAGATGGGCATGACCGTGCAGGACATCGCCCGCAATGCCGGTGATACGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCTCGCGAGGTGTTGCaa                                                                                <  2:1932946/150‑1 (MQ=255)
                                                                         cagcagGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATcc                                                                          >  1:2227121/1‑150 (MQ=255)
                                                                             agGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTc                                                                      <  2:496619/150‑1 (MQ=255)
                                                                               gagaTGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGt                                                                    >  2:1034179/1‑150 (MQ=255)
                                                                                agaTGACCGAAATGGTTGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGGCGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTg                                                                   <  1:197825/150‑1 (MQ=255)
                                                                                agaTGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTg                                                                   <  1:1237508/150‑1 (MQ=255)
                                                                                     aCCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAg                                                                                                  <  1:1867061/114‑1 (MQ=255)
                                                                                       cGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGCTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGa                                                            >  1:1845456/1‑150 (MQ=255)
                                                                                        gAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGGCCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGGCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAg                                                           >  1:1112107/1‑150 (MQ=255)
                                                                                        gAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGCTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAg                                                           <  2:1845456/150‑1 (MQ=255)
                                                                                         aaaTGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAgg                                                          >  2:2173062/1‑150 (MQ=255)
                                                                                         aaaTGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAgg                                                          <  2:2110875/150‑1 (MQ=255)
                                                                                          aaTGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggtgg                                                                                     <  1:246040/122‑1 (MQ=255)
                                                                                          aaTGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggtgg                                                                                     >  2:246040/1‑122 (MQ=255)
                                                                                          aaTGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAggg                                                         <  2:1448153/150‑1 (MQ=255)
                                                                                             ggTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCAt                                                      <  2:1072185/150‑1 (MQ=255)
                                                                                               tCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTgg                                                                  >  2:318670/1‑136 (MQ=255)
                                                                                               tCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGt                                                    >  1:1363661/1‑150 (MQ=255)
                                                                                               tCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGt                                                    >  2:1052327/1‑150 (MQ=255)
                                                                                                  gatctgctgTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAAATGCTGCGATGCTGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCgg                                                 <  1:1353465/147‑1 (MQ=255)
                                                                                                  ccACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCa                                                                                                                    <  1:806433/83‑1 (MQ=255)
                                                                                                  ccACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCa                                                                                                                    >  2:806433/1‑83 (MQ=255)
                                                                                                  ccACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCgg                                                 <  1:946954/150‑1 (MQ=255)
                                                                                                   cACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCac                                                                                                                   <  2:2399550/83‑1 (MQ=255)
                                                                                                    aCTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGGGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGAATCGAGGACATGTCGGGc                                               <  1:83127/150‑1 (MQ=255)
                                                                                                    aCTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGc                                               <  2:1159493/150‑1 (MQ=255)
                                                                                                    aCTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGc                                               <  2:197348/150‑1 (MQ=255)
                                                                                                      tgctgTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTg                                                                   >  1:1037137/1‑128 (MQ=255)
                                                                                                         tgTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACAt                                          <  1:130374/150‑1 (MQ=255)
                                                                                                           tACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCg                                                  <  1:459041/140‑1 (MQ=255)
                                                                                                           tACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCg                                                  >  2:459041/1‑140 (MQ=255)
                                                                                                           tACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCg                                        <  1:234593/150‑1 (MQ=255)
                                                                                                           tACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCAAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCg                                        <  2:2013819/150‑1 (MQ=255)
                                                                                                             cacGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGc                                      >  2:1947135/1‑150 (MQ=255)
                                                                                                             caagagaTGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGc                                      <  1:855448/147‑1 (MQ=255)
                                                                                                              acGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCg                                        <  1:2014632/147‑1 (MQ=255)
                                                                                                              acGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCg                                        >  2:2014632/1‑147 (MQ=255)
                                                                                                              acGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCa                                     >  1:840377/1‑150 (MQ=255)
                                                                                                                  gaTGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAggc                                 <  2:1136848/150‑1 (MQ=255)
                                                                                                                   aTGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAggcg                                >  2:1326084/1‑150 (MQ=255)
                                                                                                                        ccTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgagacga                                                                                                            <  1:2159615/69‑1 (MQ=255)
                                                                                                                        ccTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGa                           >  1:1959132/1‑150 (MQ=255)
                                                                                                                            aCCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCg                       >  1:596914/1‑150 (MQ=255)
                                                                                                                             ccGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCgg                      >  1:1935722/1‑150 (MQ=255)
                                                                                                                                  cAGGACATCGCCCGCAATGCCGGCGATGCGGCCCCCCGGTGGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCCAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagc                 <  1:1330625/150‑1 (MQ=255)
                                                                                                                                   aGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTgg                                                                  <  1:122483/100‑1 (MQ=255)
                                                                                                                                   aGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTgg                                                                  >  2:122483/1‑100 (MQ=255)
                                                                                                                                   aGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGAt                          <  2:2129681/140‑1 (MQ=255)
                                                                                                                                   aGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagcc                >  2:1257020/1‑150 (MQ=255)
                                                                                                                                    ccgcATCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagcca               >  2:1919872/4‑150 (MQ=255)
                                                                                                                                      gcATCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagccagc             >  1:1692349/2‑150 (MQ=255)
                                                                                                                                      gcATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagccagc             >  2:380893/2‑150 (MQ=255)
                                                                                                                                      aCATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagccagc             <  2:1846048/150‑1 (MQ=255)
                                                                                                                                        aTCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTca                   <  2:2344829/142‑1 (MQ=255)
                                                                                                                                        aTCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTca                   >  1:2344829/1‑142 (MQ=255)
                                                                                                                                        aTCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGc                        >  1:1620957/1‑137 (MQ=255)
                                                                                                                                        aTCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGc                        <  2:1620957/137‑1 (MQ=255)
                                                                                                                                         tCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATg                         >  2:1788201/1‑135 (MQ=255)
                                                                                                                                          tgaccGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGGTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGTGCATGTCGGGCGAGATTGGCAAGGCGTCCGATTCGGTGAACGACCTGGc         >  2:509507/4‑150 (MQ=255)
                                                                                                                                          cGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATc                                                                           >  2:1833740/1‑84 (MQ=255)
                                                                                                                                          cGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATc                                                                           <  1:1833740/84‑1 (MQ=255)
                                                                                                                                             ccGCATTGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCcga      <  2:869959/150‑1 (MQ=255)
                                                                                                                                              cGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGc                                                                                                                     >  2:1876645/1‑38 (MQ=255)
                                                                                                                                                 aaTGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCgg                                                 <  2:1876842/103‑1 (MQ=255)
                                                                                                                                                 aaTGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCgg                                                 >  1:1876842/1‑103 (MQ=255)
                                                                                                                                                 aaTGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCCGACGaa  <  1:1034179/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCCGACGAA  >  NC_002947/384644‑384938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑