Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 391,002 A→C 6.4% S425R (AGC→CGC)  soxA → sarcosine oxidase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947391,0020AC 6.4% 413.4 / 14.5 135S425R (AGC→CGC) soxAsarcosine oxidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (71/55);  new base C (3/6);  total (74/61)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 138 total aligned reads displayed.

GCATACTCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGATGTCATTGCCGAT  >  NC_002947/390853‑391151
                                                                                                                                                     |                                                                                                                                                     
gCATACTCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACa                                                                                                                                                       >  2:1974383/1‑150 (MQ=255)
gCATACTCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACa                                                                                                                                                       <  1:556692/150‑1 (MQ=255)
   tACTCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGcc                                                                                                                                                    <  1:2133531/150‑1 (MQ=255)
      tCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCTGTAACCGCCCGa                                                                                                                                                 <  1:715777/150‑1 (MQ=255)
      tCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGa                                                                                                                                                 >  1:1188672/1‑150 (MQ=255)
        aCCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCTGGCCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATc                                                                                                                                               <  1:1330573/150‑1 (MQ=255)
         ccTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCTGTAACCGCCCGagga                                                                                                                                              <  2:606971/150‑4 (MQ=255)
           tCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCTGTAACCGCCCGaggagg                                                                                                                                            <  1:1540409/150‑6 (MQ=255)
             cAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGACTCCTCGGGCGGTTACAGCCCGATCGTGcc                                                                                                                                          >  2:745738/1‑149 (MQ=255)
                  ccGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTgg                                                                                                                                     <  2:770012/150‑1 (MQ=255)
                  ccGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCAGATAGTGCACCTgg                                                                                                                                     <  2:2210933/150‑1 (MQ=255)
                       aTCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCg                                                                                                                                <  2:1800760/150‑1 (MQ=255)
                          gAGGCCAAAGGCAGCAAGCACATCACCGGCGCCCGTGTGACGACGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCAGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCAc                                                                                                                             >  2:130189/1‑150 (MQ=255)
                             gCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTg                                                                                                                          >  1:1776263/1‑150 (MQ=255)
                               cAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGCCCTGATCGCCGCCTCGGGCTGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTggg                                                                                                                        >  1:1142897/1‑150 (MQ=255)
                               cAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCCCCTGGCTTCGCACCTggg                                                                                                                        >  1:1112569/1‑150 (MQ=255)
                               cAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTggg                                                                                                                        >  1:1102844/1‑150 (MQ=255)
                                aaaGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGc                                                                                                                       >  1:268589/1‑150 (MQ=255)
                                aaaGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGc                                                                                                                       >  2:1551891/1‑150 (MQ=255)
                                aaaGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGc                                                                                                                       >  1:856512/1‑150 (MQ=255)
                                 aaGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGTGCg                                                                                                                      >  2:552238/1‑150 (MQ=255)
                                 aaGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCg                                                                                                                      >  2:510642/1‑150 (MQ=255)
                                    gcagcaAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCAc                                                                                                                                         >  2:558566/1‑128 (MQ=255)
                                    gcagcaAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCAc                                                                                                                                         <  1:558566/128‑1 (MQ=255)
                                      agcaagcaCGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGc                                                                                                                 <  2:1102844/150‑1 (MQ=255)
                                      agcaagcaCGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGc                                                                                                                 >  2:1080805/1‑150 (MQ=255)
                                      agcaagcaCGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGc                                                                                                                 >  2:1411861/1‑150 (MQ=255)
                                        caagcaCGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGccc                                                                                                               <  1:1510252/150‑1 (MQ=255)
                                         aagcaCGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGcc                                                                                                                                                    <  2:52153/112‑1 (MQ=255)
                                         aagcaCGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGcc                                                                                                                                                    >  1:52153/1‑112 (MQ=255)
                                               gTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTgg                                                                                                                         >  2:1007570/1‑133 (MQ=255)
                                               gTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTgg                                                                                                                         <  1:1007570/133‑1 (MQ=255)
                                               gTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGACCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATgg                                                                                                        <  2:811069/150‑1 (MQ=255)
                                                tCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGc                                                                                                       >  2:1859909/1‑150 (MQ=255)
                                                  aCCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGt                                                                                                     <  1:2049722/150‑1 (MQ=255)
                                                    cGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTga                                                                                                   <  1:756397/150‑1 (MQ=255)
                                                    cGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTga                                                                                                   <  1:790026/150‑1 (MQ=255)
                                                     ggCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTgac                                                                                                  <  2:1708777/150‑1 (MQ=255)
                                                         cccGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACa                                                                                              <  1:258148/150‑1 (MQ=255)
                                                          ccGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACAt                                                                                             >  1:1483710/1‑150 (MQ=255)
                                                               tgGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGGCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTg                                                                                        <  1:739003/150‑1 (MQ=255)
                                                                    gcgATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTc                                                                                   <  1:2279057/150‑1 (MQ=255)
                                                                    gcgATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTc                                                                                   <  1:1991609/150‑1 (MQ=255)
                                                                      gatcgatGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGt                                                                                 >  2:776082/1‑150 (MQ=255)
                                                                      gatcgatGTGCAGGCGCACAAAGTCACCAACCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGt                                                                                 >  1:1872407/1‑150 (MQ=255)
                                                                          gatGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCg                                                                             >  1:958528/1‑150 (MQ=255)
                                                                                 aGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCTGTAACCGCCCGagga                                                                                                                                              >  1:1723218/1‑75 (MQ=38)
                                                                                 aGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCTGTAACCGCCCGagga                                                                                                                                              <  2:1723218/78‑4 (MQ=38)
                                                                                 aGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATg                                                                      >  1:1806511/1‑150 (MQ=255)
                                                                                   gcgcACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATgcg                                                                    >  2:2172151/1‑150 (MQ=255)
                                                                                     gcACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCgg                                                                            <  2:1515169/140‑1 (MQ=255)
                                                                                        caAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCa                                                               <  2:1776263/150‑1 (MQ=255)
                                                                                         aaaGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAg                                                              >  2:885873/1‑150 (MQ=255)
                                                                                          aaGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGa                                                             >  2:105561/1‑150 (MQ=255)
                                                                                          aaGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGa                                                             >  2:437926/1‑150 (MQ=255)
                                                                                            gTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTgac                                                                                                  <  1:646824/111‑1 (MQ=255)
                                                                                            gTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTgac                                                                                                  >  2:646824/1‑111 (MQ=255)
                                                                                            gTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGACCGATCGTGCACCTGACTGCGCACCTGTGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAg                                                           >  1:900082/1‑150 (MQ=255)
                                                                                             tCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGc                                                          <  1:105561/150‑1 (MQ=255)
                                                                                               aCCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGcgcc                                                                  <  2:952454/140‑1 (MQ=255)
                                                                                               aCCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGcgcc                                                                  >  1:952454/1‑140 (MQ=255)
                                                                                               aCCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGt                                                        <  2:1142897/150‑1 (MQ=255)
                                                                                                 cAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGa                                                      <  1:1080805/150‑1 (MQ=255)
                                                                                                  aGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAg                                                     <  1:2103628/150‑1 (MQ=255)
                                                                                                    cccAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTg                                                   >  1:1736764/1‑150 (MQ=255)
                                                                                                     ccAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGc                                                  <  1:2106505/150‑1 (MQ=255)
                                                                                                     ccAGGCGAAGTCCTTGAGTGCGACCTGATCGACTCATCGGGCGGTTAACGGCCGATCGTGCACCTGGCTTCGCACCTGGGCGATCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGc                                                  >  1:658838/1‑150 (MQ=255)
                                                                                                       aGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCtt                                                                                         <  2:1810033/109‑1 (MQ=255)
                                                                                                         gCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCg                                              >  2:1258183/1‑150 (MQ=255)
                                                                                                           gAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTAGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggt                                            <  1:1974383/150‑1 (MQ=255)
                                                                                                           gAAGTCCTTGAGTGCGACCTGATCACCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggt                                            >  2:232244/1‑150 (MQ=255)
                                                                                                                ccTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGATCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTAt                                       <  2:658838/150‑1 (MQ=255)
                                                                                                                  ttGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCa                                     >  2:614330/1‑150 (MQ=255)
                                                                                                                  ttGAGTACAACATGACCGCCTCCTCGGTCGTTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGCGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggtcggagca                                     >  2:150564/1‑143 (MQ=255)
                                                                                                                     aGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGATTGCGTCGGTGGTATCAACg                                  <  1:552238/150‑1 (MQ=255)
                                                                                                                      gTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACgg                                 <  1:510642/150‑1 (MQ=255)
                                                                                                                       tGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGc                                >  1:2328755/1‑150 (MQ=255)
                                                                                                                            ccTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGTTATCAACGGCGTGTa                           <  2:958528/150‑1 (MQ=255)
                                                                                                                             cTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGAGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTAt                          >  2:1559911/1‑150 (MQ=255)
                                                                                                                               gATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGc                        <  2:205778/150‑1 (MQ=255)
                                                                                                                                aTCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGcc                       >  2:2327334/1‑150 (MQ=255)
                                                                                                                                 tCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCt                      >  1:315495/1‑150 (MQ=255)
                                                                                                                                    cctcctCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTga                                                                                                   <  1:1003610/70‑1 (MQ=255)
                                                                                                                                    cctcctCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTga                                                                                                   >  2:1003610/1‑70 (MQ=255)
                                                                                                                                    cacgatcGGGCTGTAACCGCCCGATCGTGCGCCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTg                    >  2:1278807/6‑149 (MQ=255)
                                                                                                                                    cacgatcGGGCTGTAACCGCCCGATCGTGCGCCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTg                    <  1:1278807/144‑1 (MQ=255)
                                                                                                                                     ctcctcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATgg                                                                                                        >  2:1675933/1‑64 (MQ=255)
                                                                                                                                     ctcctcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATgg                                                                                                        <  1:1675933/64‑1 (MQ=255)
                                                                                                                                     ctcctcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGa                                                                        >  2:719989/1‑96 (MQ=255)
                                                                                                                                      tcctcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCTCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGc                 >  2:88706/1‑150 (MQ=255)
                                                                                                                                      tcctcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGc                 >  1:1506733/1‑150 (MQ=255)
                                                                                                                                        ctcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGa               >  1:1837392/1‑150 (MQ=255)
                                                                                                                                        ctcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGa               >  1:1526037/1‑150 (MQ=255)
                                                                                                                                        ctcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGa               >  1:1386084/1‑150 (MQ=255)
                                                                                                                                         tcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGAt              <  2:2012982/150‑1 (MQ=255)
                                                                                                                                          cGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGATg             <  2:1143673/150‑1 (MQ=255)
                                                                                                                                          cGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGATg             <  2:2328755/150‑1 (MQ=255)
                                                                                                                                            ggCGGTTACAGCCCGATCGTGCACCTGGCCTCGCACGTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGATGTc           <  2:1112569/150‑1 (MQ=255)
                                                                                                                                             gCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGa                                                      <  2:1785185/106‑1 (MQ=255)
                                                                                                                                             gCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGa                                                      >  1:1785185/1‑106 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCATACTCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGCGGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGATGTCATTGCCGAT  >  NC_002947/390853‑391151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑