Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,927,140 T→G 5.1% K263Q (AAG→CAG)  cheBA ← chemotaxis response regulator protein‑glutamate methylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,927,1400TG 5.1% 356.5 / 8.3 117K263Q (AAG→CAG) cheBAchemotaxis response regulator protein‑glutamate methylesterase
Reads supporting (aligned to +/- strand):  ref base T (51/60);  new base G (2/4);  total (53/64)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 121 total aligned reads displayed.

GCCCATGCCGGTGAGCACCACCGACAACACCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGATCAACACGATCGGCGC  >  NC_002947/4926991‑4927287
                                                                                                                                                     |                                                                                                                                                   
gcCCATGCCGGTGAGCACCACCGACAACACCTTGTCACCGTAAGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCtt                                                                                                                                                     >  1:277083/1‑150 (MQ=255)
  ccATGCCGGTGATCACCACCGACAGCACCTTGTCACCGTACGACTTGGCCACCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGc                                                                                                                                                   <  2:1038813/150‑1 (MQ=255)
        cGGTGAGCACCACCGACAACACCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCCCCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCgg                                                                                                                                             >  1:831008/1‑150 (MQ=255)
        cGGTGAGCACCACCGACAACACCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCgg                                                                                                                                             <  2:1188702/150‑1 (MQ=255)
                  caccGTCAACACCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCcagcag                                                                                                                                   >  2:603396/1‑150 (MQ=255)
                  caccGACAACACCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCcagcag                                                                                                                                   >  1:13117/1‑150 (MQ=255)
                   accGACAACACCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAgg                                                                                                                                  <  1:2184078/150‑1 (MQ=255)
                    ccGACAACACCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCCGCAggg                                                                                                                                 >  2:1103162/1‑150 (MQ=255)
                    ccGACAACACCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAggg                                                                                                                                 <  2:1918704/150‑1 (MQ=255)
                        caacaCCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAg                                                                                                                             <  2:1065799/150‑1 (MQ=255)
                         aacaCCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCcagca                                                                                                                                    <  1:278719/142‑1 (MQ=255)
                         aacaCCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAgg                                                                                                                            <  2:13117/150‑1 (MQ=255)
                           cacCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGAGTATCGTCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGcc                                                                                                                          >  1:262271/1‑150 (MQ=255)
                            acCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGcccc                                                                                                                         >  1:944178/1‑150 (MQ=255)
                            acCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTGGCCCCCGGTGGCaagc                                                                                                                                     >  1:1562606/1‑138 (MQ=255)
                            acCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTGGCCCCCGGTGGCaagc                                                                                                                                     <  2:1562606/138‑1 (MQ=255)
                              cTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATTATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCgg                                                                                                                       <  2:1394957/150‑1 (MQ=255)
                              cTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTGGCCCCCGGTGGCaagcagatg                                                                                                                                <  1:1018678/141‑4 (MQ=255)
                              cTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTGGCCCCCGGTGGCaagcagatg                                                                                                                                >  2:1018678/1‑138 (MQ=255)
                               ttGTCACCGTACGACTTGGCCGCAGAACCGAAGGTGATTTCCACGCACGGCTTGTAGTTAATGAAGTCGCACGCTGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCggg                                                                                                                      <  1:1251754/150‑1 (MQ=255)
                                 gTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGGAGGATCTTCACCGTAGAAAGGCCAATCTTCACCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGgcg                                                                                                                    >  1:1741751/1‑150 (MQ=255)
                                     ccggatcaCTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCgcagc                                                                                                                <  2:262271/143‑1 (MQ=255)
                                       gTACGACTTGGCCGCCGCACAGAAGGTGATGGCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCAGAGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCAt                                                                                                              <  1:1731212/150‑1 (MQ=255)
                                            aCTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGCTCACAGGGTGCGTTGCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGc                                                                                                         >  2:65061/1‑150 (MQ=255)
                                                  ccgccgAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAgg                                                                                                                                  <  2:2235779/119‑1 (MQ=255)
                                                  ccgccgAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAgg                                                                                                                                  >  1:2235779/1‑119 (MQ=255)
                                                   cgccgAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTc                                                                                                  <  2:1142287/150‑1 (MQ=255)
                                                    gccgAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCTTTGGGCAGCATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCg                                                                                                 <  1:1132086/150‑1 (MQ=255)
                                                    gccgAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCg                                                                                                 <  1:59434/150‑1 (MQ=255)
                                                    gccgAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTCCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCg                                                                                                 <  2:277083/150‑1 (MQ=255)
                                                        aaccgaaGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCtt                                                                                             >  2:2388761/1‑150 (MQ=255)
                                                         accgaaGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTc                                                                                                  >  1:1694960/1‑144 (MQ=255)
                                                         accgaaGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTc                                                                                                  <  2:1694960/144‑1 (MQ=255)
                                                           cgaaGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCgggg                                                                                                                                           <  2:2061638/101‑1 (MQ=255)
                                                           cgaaGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCgggg                                                                                                                                           >  1:2061638/1‑101 (MQ=255)
                                                           cgaaGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAACGCGAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCt                                                                                          >  1:1833251/1‑150 (MQ=255)
                                                            gaaGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCtt                                                                                         <  2:1065376/150‑1 (MQ=255)
                                                              aGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGa                                                                                       <  1:2388761/150‑1 (MQ=255)
                                                               ggTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGAc                                                                                      <  1:861631/150‑1 (MQ=255)
                                                                 tGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGc                                                                                    >  2:2389187/1‑150 (MQ=255)
                                                                  gATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCggg                                                                                                                                            >  1:370247/1‑93 (MQ=255)
                                                                  gATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCggg                                                                                                                                            <  2:370247/93‑1 (MQ=255)
                                                                       ccACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATg                                                                               <  2:2200139/149‑1 (MQ=255)
                                                                       ccACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATg                                                                               >  1:2200139/1‑149 (MQ=255)
                                                                       ccACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGc                                                                              <  2:557140/150‑1 (MQ=255)
                                                                        cacgcacgGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTcc                                                                                           <  1:932191/136‑1 (MQ=255)
                                                                        cacgcacgGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTcc                                                                                           >  2:932191/1‑136 (MQ=255)
                                                                        cacgcacgGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCg                                                                             >  1:592241/1‑150 (MQ=255)
                                                                             acgGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCggg                                                                                                                      <  1:488298/104‑1 (MQ=255)
                                                                             acgGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCggg                                                                                                                      >  2:488298/1‑104 (MQ=255)
                                                                              cgGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAg                                                                       <  2:961191/150‑1 (MQ=255)
                                                                                  ttGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCgg                                                                            >  2:536574/1‑141 (MQ=255)
                                                                                  ttGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCgg                                                                            <  1:536574/141‑1 (MQ=255)
                                                                                    gTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTTCCACCGGGCGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTc                                                                 <  2:74769/150‑1 (MQ=255)
                                                                                    gTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCgg                                                                                                                       >  1:100354/1‑96 (MQ=255)
                                                                                    gTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTc                                                                 >  1:824470/1‑150 (MQ=255)
                                                                                    gTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTc                                                                 <  2:1362957/150‑1 (MQ=255)
                                                                                      aGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGGCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCa                                                               <  2:559745/150‑1 (MQ=255)
                                                                                       gTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGa                                                                                 >  2:2370836/1‑131 (MQ=255)
                                                                                       gTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGa                                                                                 <  1:2370836/131‑1 (MQ=255)
                                                                                         tCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGc                                                            <  1:1396172/150‑1 (MQ=255)
                                                                                          cAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCAt                                                                                                              >  1:332298/1‑99 (MQ=255)
                                                                                          cAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCAt                                                                                                              <  2:332298/99‑1 (MQ=255)
                                                                                             gcgcgcGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGCTGCGGGACAGCTTGTCCAGGCGTTc                                                        <  2:1421269/150‑1 (MQ=255)
                                                                                             gcgcTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCgca                                                                                                                  <  2:187931/92‑1 (MQ=255)
                                                                                             gcgcTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCgca                                                                                                                  >  1:187931/1‑92 (MQ=255)
                                                                                                 tcgtcgCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCa                                                    >  1:2283831/1‑150 (MQ=255)
                                                                                                   gtcgCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAg                                                              >  1:1525399/1‑138 (MQ=255)
                                                                                                         gggCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCttt                                            <  2:824470/150‑1 (MQ=255)
                                                                                                                aTCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCGTGTCGCCGTCTTCGGCTTCCTTGACGCTGATGc                                                                              <  2:801940/109‑1 (MQ=255)
                                                                                                                aTCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCGTGTCGCCGTCTTCGGCTTCCTTGACGCTGATGc                                                                              >  1:801940/1‑109 (MQ=255)
                                                                                                                aTCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAg                                     >  1:485323/1‑150 (MQ=255)
                                                                                                                 tCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATCAGCCACAGCATATCTAAGCGTTCTGCAAACGCTCTGGGGaatg                                    >  2:522436/1‑148 (MQ=255)
                                                                                                                 tCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCATTGAGGCTTATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAgg                                    <  2:944178/150‑1 (MQ=255)
                                                                                                                   ttCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGc                                                            >  2:1061180/1‑124 (MQ=255)
                                                                                                                   ttCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGc                                                            <  1:1061180/124‑1 (MQ=255)
                                                                                                                   ttCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGgcc                                  >  1:1970372/1‑150 (MQ=255)
                                                                                                                         gTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGc                            >  2:1167301/1‑150 (MQ=255)
                                                                                                                           gCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCAt                          <  2:381582/150‑1 (MQ=255)
                                                                                                                             cACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGTAAACGCTTTGGTGAAGGCCGCCGGCAtgt                        <  1:65061/150‑1 (MQ=255)
                                                                                                                              aCGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCAtgtg                       >  1:1945848/1‑150 (MQ=255)
                                                                                                                               cGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGc                      <  2:2156535/150‑1 (MQ=255)
                                                                                                                                 gCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTg                    <  2:2283831/150‑1 (MQ=255)
                                                                                                                                  ccATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAg                                     >  1:2265681/1‑132 (MQ=255)
                                                                                                                                  ccATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAg                                     <  2:2265681/132‑1 (MQ=255)
                                                                                                                                   cATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGa                  >  1:18272/1‑150 (MQ=255)
                                                                                                                                     tCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACTCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGATc                >  1:13985/1‑150 (MQ=255)
                                                                                                                                     tCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGATc                <  2:1970372/150‑1 (MQ=255)
                                                                                                                                       gATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGgccgcc                               >  1:762182/1‑133 (MQ=255)
                                                                                                                                       gATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGgccgcc                               <  2:762182/133‑1 (MQ=255)
                                                                                                                                         tcatcatcTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTg                                           <  2:174430/119‑1 (MQ=255)
                                                                                                                                         tcatcatcTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGATCAaca            <  1:1079182/150‑1 (MQ=255)
                                                                                                                                             catcTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCAt                          >  1:1869185/1‑132 (MQ=255)
                                                                                                                                              atcTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGATCAACACGATc       <  2:18272/150‑1 (MQ=255)
                                                                                                                                              atcTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGATCAACACGATc       >  2:734073/1‑150 (MQ=255)
                                                                                                                                               tcTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGATCAACACGATCg      <  2:902338/150‑1 (MQ=255)
                                                                                                                                               cctgctgGCCCCCGGTGGCAAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTg                                                                   >  1:650986/2‑89 (MQ=38)
                                                                                                                                               cctgctgGCCCCCGGTGGCAAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGATCAACACGATCg      >  2:1396302/2‑150 (MQ=255)
                                                                                                                                                 tGCTGGCCCCCGGTGGCAAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGATCAACACGATCGgc    <  1:1396302/150‑1 (MQ=255)
                                                                                                                                                   cTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGATCAACACGATCGgcgc  >  2:1753475/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCCCATGCCGGTGAGCACCACCGACAACACCTTGTCACCGTACGACTTGGCCGCCGAACCGAAGGTGATGTCCACGCACGGCTTGTAGTTCAGGCGCTCGTCGCCGGGCAGGATCTTCACCGTGCCACGGCCATCGATCATCATCTGCTTGCCACCGGGGGCCAGCAGGGCCAGGCCCGGGCGCAGCATGTCGCCGTCTTCGGCTTCCTTGACGCTGATGCGGCACAGCTTGTCCAGGCGTTCGGCAAACGCTTTGGTGAAGGCCGCCGGCATGTGCTGGATCAACACGATCGGCGC  >  NC_002947/4926991‑4927287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑