Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,970,038	T→G	8.3%	T84P (ACT→CCT)	fliC ←	flagellin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,970,038	0	T	G	8.3%	232.0 / 11.4	86	T84P (ACT→CCT)	fliC	flagellin
Reads supporting (aligned to +/- strand):  ref base T (40/38);  new base G (4/3);  total (45/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

CCGAAAGTGGTGGTGTTAGCAATACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGATTTTCAG  >  NC_002947/4969890‑4970182                                                                                                                                                     |                                                                                                                                                 ccGAAAGTGGTGGTGTTAGCAATACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTc                                                                                                                                                 <  2:924034/150‑1 (MQ=255) ccGAAAGTGGTGGTGTTAGCAATACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTc                                                                                                                                                 >  1:1393470/1‑150 (MQ=255)   gAAAGTGGTGGTGTTAGCAATACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGc                                                                                                                                               <  1:2169406/150‑1 (MQ=255)        tggtggtgTTAGCAATACGGGTGAGATCGCCGGAGCGTCCAGTAAGTCGGTACTGCCGCTATTGGCGGTTAGCGTGGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATgg                                                                                                                                          >  1:657491/1‑150 (MQ=255)           tggtgTTAGCAATACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGgcgc                                                                                                                                       <  2:510820/150‑1 (MQ=255)                ttAGCAATACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGGCGCCATGGCGACTTca                                                                                                                                  >  1:561022/1‑150 (MQ=255)                 tAGCAATACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGGCGCCATGGCGACTTcag                                                                                                                                 <  2:561022/150‑1 (MQ=255)                 tAGCAATACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCCGCAACATTGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGGCGCCATGGCGACTTcag                                                                                                                                 <  1:1352825/150‑1 (MQ=255)                  aGCAATACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTcagc                                                                                                                                >  2:234903/1‑150 (MQ=255)                   gCAATACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTcagca                                                                                                                               <  1:998584/150‑1 (MQ=255)                      aTACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGtt                                                                                                                            >  1:1427320/1‑150 (MQ=255)                      aTACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGtt                                                                                                                            >  1:2003517/1‑150 (MQ=255)                            gTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCa                                                                                                                      >  2:1700419/1‑150 (MQ=255)                               aGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTCCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATa                                                                                                                   <  1:338767/150‑1 (MQ=255)                                gCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATag                                                                                                                  >  1:2239734/1‑150 (MQ=255)                                  tCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATagag                                                                                                                >  2:1590453/1‑150 (MQ=255)                                  tCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATagag                                                                                                                <  1:1590453/150‑1 (MQ=255)                                    gCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGAt                                                                                                              >  2:1691236/1‑150 (MQ=255)                                      ccGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATa                                                                                                                   >  2:2082804/1‑143 (MQ=255)                                      ccGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATa                                                                                                                   <  1:2082804/143‑1 (MQ=255)                                      ccGACAGCGCGGTAAACTCCTGCTGCATGGAGGACAGGTACCCATTGACATTGGAGCCGTTCGCGGATGTGGTCGCCAGTTCGCGAATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATAc                                                                                                            >  1:45416/1‑150 (MQ=255)                                            gcgcGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGt                                                                                                      >  1:2264316/1‑150 (MQ=255)                                                ggTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGc                                                                                                  <  1:71029/150‑1 (MQ=255)                                                      cTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGc                                                                                                  >  1:1040488/1‑144 (MQ=255)                                                      cTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGc                                                                                                  <  2:1040488/144‑1 (MQ=255)                                                      cTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATACAGATACCACCGTTGGCGTTCtt                                                                                            >  2:224376/1‑150 (MQ=255)                                                       tCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTg                                                                                           >  2:1228730/1‑150 (MQ=255)                                                        ccTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGa                                                                                          <  1:1228730/150‑1 (MQ=255)                                                         ctgctgCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGAc                                                                                         >  1:1100598/1‑150 (MQ=255)                                                          tgctgcATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGAcc                                                                                        >  1:1002308/1‑150 (MQ=255)                                                            ctgcATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGGTTTGACCCTTTGCTCCGATGGCTCCTGCAGCAGTTTGTGCAACAGAGATACCATCGCTGGCGTTCTTGACCGc                                                                                      >  1:1252007/1‑150 (MQ=255)                                                            ctgcATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGgggc                                                                                                                                       <  1:696347/101‑1 (MQ=255)                                                            ctgcATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGgcgc                                                                                                                                       >  2:696347/1‑101 (MQ=255)                                                            ctgcATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGc                                                                                      >  2:2398504/1‑150 (MQ=255)                                                              gcATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGa                                                                                          >  2:1238667/1‑144 (MQ=255)                                                              gcATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGa                                                                                          <  1:1238667/144‑1 (MQ=255)                                                                         cgGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCt                                                                         <  1:1691236/150‑1 (MQ=255)                                                                           gtcgtcATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCttt                                                                       <  2:2003517/150‑1 (MQ=255)                                                                            tcgtcATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCtttg                                                                      >  1:1725970/1‑150 (MQ=255)                                                                              gtcATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAAt                                                                                                                    >  2:70122/1‑102 (MQ=255)                                                                              gtcATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAAt                                                                                                                    <  1:70122/102‑1 (MQ=255)                                                                               tcATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCtttgatt                                                                   <  2:1002308/150‑1 (MQ=255)                                                                                 aTCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCtttgatttg                                                                 >  1:2061471/1‑150 (MQ=255)                                                                                 aTCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGGCGCCATGGCGACTTca                                                                                                                                  <  2:1587802/85‑1 (MQ=255)                                                                                 aTCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGGCGCCATGGCGACTTca                                                                                                                                  >  1:1587802/1‑85 (MQ=255)                                                                                  tCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCtttgatttga                                                                <  1:1282702/150‑1 (MQ=255)                                                                                    gCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGt                                                              <  2:1722094/150‑1 (MQ=255)                                                                                    gCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGt                                                              >  1:88305/1‑150 (MQ=255)                                                                                    gCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGt                                                              <  2:2264316/150‑1 (MQ=255)                                                                                           gAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGa                                                       <  1:2398504/150‑1 (MQ=255)                                                                                                gTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGtt                                                  <  2:2061471/150‑1 (MQ=255)                                                                                                 ttCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGAATTGAGTGGTCAGACGGTTg                                                 >  2:1588656/1‑150 (MQ=255)                                                                                                   cGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACTGTTGga                                               <  2:1100598/150‑1 (MQ=255)                                                                                                     ccGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGgaga                                             >  2:255819/1‑150 (MQ=255)                                                                                                      cGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGAt                                            >  1:1042071/1‑150 (MQ=255)                                                                                                       gATTGCAGGGCCAGTCCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATc                                           <  2:657491/150‑1 (MQ=255)                                                                                                        aTTGCAGGGCCAGTTCACGCATACGCTGCATGAAGTTGCCTGAAGTCGCCATTGCGCCTTCACCAGTTTGGGCAATAGAGATACCATCGTTGGCGTGCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTAAAAGATCt                                          >  2:958722/1‑150 (MQ=255)                                                                                                        aTTGAAGGGCCAGGTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTAAGACGGTTGGAGATCt                                          <  2:45416/150‑1 (MQ=255)                                                                                                          tGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGc                                        <  2:1252007/150‑1 (MQ=255)                                                                                                            cAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAg                                      <  2:88305/150‑1 (MQ=255)                                                                                                            cAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAg                                      <  2:1042071/150‑1 (MQ=255)                                                                                                             aGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGc                                                                                                  <  1:2013680/89‑1 (MQ=255)                                                                                                             aGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGc                                                                                                  >  2:2013680/1‑89 (MQ=255)                                                                                                              gggCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGc                                    <  2:1937304/150‑1 (MQ=255)                                                                                                                     ttCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTgcggc                             <  1:255819/150‑1 (MQ=255)                                                                                                                     ttCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTgcggc                             <  1:1588656/150‑1 (MQ=255)                                                                                                                        aCGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGcgtc                          >  1:1019225/1‑150 (MQ=255)                                                                                                                          gCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATaga                                                                                                                 >  2:1760813/1‑61 (MQ=255)                                                                                                                          gCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATaga                                                                                                                 <  1:1760813/61‑1 (MQ=255)                                                                                                                          gCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGcgtcgt                        >  2:505968/1‑150 (MQ=255)                                                                                                                            aTACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCt                      >  1:1552107/1‑150 (MQ=255)                                                                                                                             tACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCtt                     >  1:1358863/1‑150 (MQ=255)                                                                                                                              aCGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTAAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCttt                    <  1:224376/150‑1 (MQ=255)                                                                                                                                   gCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCAc               >  1:986845/1‑150 (MQ=255)                                                                                                                                    cATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCAt                                                                                                         <  1:2087782/59‑1 (MQ=255)                                                                                                                                    cATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCAt                                                                                                         >  2:2087782/1‑59 (MQ=255)                                                                                                                                         tgTTGCTTGAAGTCGCAATGGCGCCTTCAGCAGCTTGCGAAATAGGGGTACCATCGGTGGCGTTCTTGACCGCACCGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGa         <  1:958722/150‑1 (MQ=255)                                                                                                                                         tgTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGa         <  1:1700419/150‑1 (MQ=255)                                                                                                                                           ttGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGAtt       <  1:886831/150‑1 (MQ=255)                                                                                                                                              ccTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGATTTTc    >  2:578689/1‑150 (MQ=255)                                                                                                                                              ccTGAAGTCGCCATGGCGCCTTCAGCAGCTTGCGCAATAGAGATACCATCGTTGGGCGTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGATTTTc    <  1:636427/150‑1 (MQ=255)                                                                                                                                               cTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGATTTTCa   >  2:320646/1‑150 (MQ=255)                                                                                                                                               cTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGATTTTCa   >  2:1748766/1‑150 (MQ=255)                                                                                                                                               cTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGATCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGATTTTCa   >  2:1559078/1‑150 (MQ=255)                                                                                                                                                tGAAGGCGCCATGGCGACTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGATTTTCAg  >  1:780701/1‑150 (MQ=255)                                                                                                                                                tGAAGGCGCCATGGCGACTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGATTTTCAg  >  1:2252029/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CCGAAAGTGGTGGTGTTAGCAATACGGGTGAGCTCGCCCGACAGCGCGGTAAACTCCTGCTGCATGGAGGCGCGGTCGTCATCGCTGTTCGAGCCGTTCGCCGATTGCAGGGCCAGTTCACGCATACGCTGCATGATGTTGCCTGAAGTCGCCATGGCGCCTTCAGCAGTTTGCGCAATAGAGATACCATCGTTGGCGTTCTTGACCGCAACGCTCAGACCTTTGATTTGAGTGGTCAGACGGTTGGAGATCTGCAGGCCTGCGGCGTCGTCTTTGGCACTGTTGATTTTCAG  >  NC_002947/4969890‑4970182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑