BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,013,335	G→C	6.2%	L36L (CTG→CTC)	PP_5678 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,013,335	0	G	C	6.2%	437.2 / 12.5	143	L36L (CTG→CTC)	PP_5678	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (67/64); new base C (3/6); total (71/72)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

ACCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTTACTCT  >  NC_002947/5013187‑5013483

aCCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGa                                                                                                                                                     >  1:979264/1‑150 (MQ=255)

aCCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGa                                                                                                                                                     >  1:1990931/1‑150 (MQ=255)

aCCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGGCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGa                                                                                                                                                     >  2:1890319/1‑150 (MQ=255)

  ccGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCAACAGCACGCCCTGTGGAgc                                                                                                                                                   >  1:238471/1‑150 (MQ=255)

     aGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGcgcg                                                                                                                                                <  1:1035859/150‑1 (MQ=255)

       cAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCacggc                                                                                                                                              <  1:1483359/150‑1 (MQ=255)

         gTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGt                                                                                                                                            <  1:416215/150‑1 (MQ=255)

         gTGCAACAAGCCTCAAACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGCCCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACTGCACGCCGCGCTCAGCACGGCGt                                                                                                                                            <  2:844074/150‑1 (MQ=255)

            caacaaGCCTCAGAGTTAGGAGAGATCCAATGATCATCAAAGTGTAGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCCCACTTGTCCTTCTGTTGACGGCCATCCACAGCACGCCGTGCTCAGCACGGCGTgct                                                                                                                                         <  2:337476/150‑3 (MQ=255)

            caacaaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGa                                                                                                                                         >  2:1548732/1‑150 (MQ=255)

               caaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACACCACGCCGTGCTGAGCGCGGCGTGGAAAt                                                                                                                                      <  2:689391/150‑1 (MQ=255)

                aaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATc                                                                                                                                     <  1:1342413/150‑1 (MQ=255)

                    cTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGc                                                                                                                                 >  2:2280457/1‑150 (MQ=255)

                    cTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGc                                                                                                                                 >  2:792222/1‑150 (MQ=255)

                     tCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCa                                                                                                                                    >  2:1224105/1‑146 (MQ=255)

                         aCTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCgca                                                                                                                            <  2:242614/150‑1 (MQ=255)

                         aCTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCgca                                                                                                                            <  2:1358802/150‑1 (MQ=255)

                           ttAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCTCAGCACGGCGTGCTgtgga                                                                                                                                                  <  2:2370895/126‑5 (MQ=255)

                           ttAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCTCAGCACGGCGTGCTgtgga                                                                                                                                                  >  1:2370895/1‑122 (MQ=255)

                           ttAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCgcagc                                                                                                                          >  1:1806080/1‑150 (MQ=255)

                                 gagaGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGTTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAAcc                                                                                                                    <  1:805639/150‑1 (MQ=255)

                                    agCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTgc                                                                                                                                          >  1:2398885/1‑124 (MQ=255)

                                      ccAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGaa                                                                                                               <  2:1990931/150‑1 (MQ=255)

                                      ccAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCCCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGaa                                                                                                               >  2:271253/1‑150 (MQ=255)

                                       cAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTAAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAg                                                                                                              >  1:1189958/1‑150 (MQ=255)

                                        aaTGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATTACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGaa                                                                                                               >  2:577613/1‑148 (MQ=255)

                                        aaTGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATTACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGaa                                                                                                               <  1:577613/148‑1 (MQ=255)

                                           gatgatCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTa                                                                                                          <  1:1533477/150‑1 (MQ=255)

                                            atgatCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGccgaa                                                                                                         <  1:1069943/150‑4 (MQ=255)

                                            atgatCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTAt                                                                                                         <  1:926366/150‑1 (MQ=255)

                                              gatCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGCAGCCTATGa                                                                                                       >  2:326456/1‑150 (MQ=255)

                                                  aaaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTc                                                                                                   >  2:1156618/1‑150 (MQ=255)

                                                   aaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTcc                                                                                                  >  1:1369977/1‑150 (MQ=255)

                                                   aaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTcc                                                                                                  >  1:1305400/1‑150 (MQ=255)

                                                   aaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTcc                                                                                                  >  1:1919411/1‑150 (MQ=255)

                                                   aaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTcc                                                                                                  >  2:914568/1‑150 (MQ=255)

                                                   aaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTcc                                                                                                  <  1:1548732/150‑1 (MQ=255)

                                                    agagTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCa                                                                                                 >  1:425983/1‑150 (MQ=255)

                                                     gagTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCAt                                                                                                >  2:80462/1‑150 (MQ=255)

                                                          gACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTg                                                                                           <  2:434299/150‑1 (MQ=255)

                                                            cccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAgg                                                                                                                                  <  1:580059/109‑1 (MQ=255)

                                                            cccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAgg                                                                                                                                  >  2:580059/1‑109 (MQ=255)

                                                             ccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCg                                                                                                                 >  2:1795805/1‑125 (MQ=255)

                                                             ccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCg                                                                                                                 <  1:1795805/125‑1 (MQ=255)

                                                             ccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCga                                                                                        >  2:1112623/1‑150 (MQ=255)

                                                               tCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAg                                                                                                                                   <  2:250168/105‑1 (MQ=255)

                                                               tCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAg                                                                                                                                   >  1:250168/1‑105 (MQ=255)

                                                               tCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGTCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCgaga                                                                                      >  2:963988/1‑150 (MQ=255)

                                                                cGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGagag                                                                                     <  2:1327802/150‑1 (MQ=255)

                                                                   aGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCAAAGCCTATGAACTCCATCTTTGCGAGAgctg                                                                                  >  1:219492/1‑150 (MQ=255)

                                                                    gCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAgctgc                                                                                 <  1:325922/150‑1 (MQ=255)

                                                                    gCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAgctgc                                                                                 <  2:764613/150‑1 (MQ=255)

                                                                           cGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTg                                                                          <  2:1465398/150‑1 (MQ=255)

                                                                            ggTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGc                                                                         <  2:979264/150‑1 (MQ=255)

                                                                             gTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTCGAAATTAGGCGCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGcc                                                                        >  2:1279783/1‑150 (MQ=255)

                                                                                aGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACg                                                                     >  2:1546177/1‑150 (MQ=255)

                                                                                      gTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCa                                                               <  1:956652/150‑1 (MQ=255)

                                                                                       tttGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGCAGCCTATGAACTACATCTCTGCGCATTCTGCTTCTTTGCCACGCTTTCaa                                                              <  1:1261443/150‑1 (MQ=255)

                                                                                               cGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCAAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAg                                                      <  2:219492/150‑1 (MQ=255)

                                                                                                  tGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAGCGATAAAGCGt                                                   <  2:1305400/150‑1 (MQ=255)

                                                                                                      gCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAAc                                               >  1:691080/1‑150 (MQ=255)

                                                                                                      gCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAAc                                               >  1:1393257/1‑150 (MQ=255)

                                                                                                       ccAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACg                                              >  1:1972876/1‑150 (MQ=255)

                                                                                                        cAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGc                                             >  2:1105817/1‑150 (MQ=255)

                                                                                                        cAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGc                                             <  2:1393257/150‑1 (MQ=255)

                                                                                                        cAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGc                                             >  1:1514498/1‑150 (MQ=255)

                                                                                                          gACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTg                                           <  1:1156618/150‑1 (MQ=255)

                                                                                                            cTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTggg                                         <  1:1112623/150‑1 (MQ=255)

                                                                                                                 ccTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCTAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCaa                                    <  2:468003/150‑1 (MQ=255)

                                                                                                                  cTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGt                                                   <  1:1203646/134‑1 (MQ=255)

                                                                                                                  cTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGt                                                   >  2:1203646/1‑134 (MQ=255)

                                                                                                                   ttCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTTCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAAtt                                  >  2:273015/1‑150 (MQ=255)

                                                                                                                   ttCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAAtt                                  >  2:125779/1‑150 (MQ=255)

                                                                                                                     cTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGa                                <  2:1972876/150‑1 (MQ=255)

                                                                                                                     cTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGa                                <  1:273015/150‑1 (MQ=255)

                                                                                                                     cTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGa                                <  1:1890319/150‑1 (MQ=255)

                                                                                                                      tgatgaCGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCga                                                                                        <  2:775741/93‑1 (MQ=255)

                                                                                                                      tgatgaCGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCga                                                                                        >  1:775741/1‑93 (MQ=255)

                                                                                                                      tgatgaCGGCCATCCACAGCACGCCGTGCTAAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGAt                               <  2:1189958/150‑1 (MQ=255)

                                                                                                                       gatgaCGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTg                                                                          >  1:1466343/1‑106 (MQ=255)

                                                                                                                       gatgaCGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTg                                                                          <  2:1466343/106‑1 (MQ=255)

                                                                                                                        atgaCGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTg                                           >  1:763547/1‑136 (MQ=255)

                                                                                                                              gCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATaaa                                                       >  1:686270/1‑118 (MQ=255)

                                                                                                                              gCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATaaa                                                       <  2:686270/118‑1 (MQ=255)

                                                                                                                              gCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTg                       >  2:2300852/1‑150 (MQ=255)

                                                                                                                               ccATCCACAGCACGCCGTGGTGGGCGCGGAGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGa                      <  2:238471/150‑1 (MQ=255)

                                                                                                                               ccATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATTTTCTCTGa                      >  2:285525/1‑150 (MQ=255)

                                                                                                                                    cacaGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAg                 >  1:2260685/1‑150 (MQ=255)

                                                                                                                                      caGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCt               <  1:1062404/150‑1 (MQ=255)

                                                                                                                                       aGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTg              >  1:1646367/1‑150 (MQ=255)

                                                                                                                                        gCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCga                                                                                        >  2:2176761/1‑75 (MQ=255)

                                                                                                                                         cACGCCGCGCTCAGCACGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGa                      >  1:2051555/1‑140 (MQ=255)

                                                                                                                                          aCGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGcttc                                                                              >  1:991695/1‑83 (MQ=255)

                                                                                                                                            gCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTcc         >  2:2044271/1‑150 (MQ=255)

                                                                                                                                              cGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCtt       <  2:1806080/150‑1 (MQ=255)

                                                                                                                                                tGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAAGTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTTAc     <  1:2300852/150‑1 (MQ=255)

                                                                                                                                                 gCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTTAct    >  2:1880965/1‑150 (MQ=255)

                                                                                                                                                   tGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTgg                                          <  2:735450/110‑1 (MQ=255)

                                                                                                                                                   tGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTgg                                          >  1:735450/1‑110 (MQ=255)

                                                                                                                                                   tGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTTActct  >  1:854350/1‑150 (MQ=255)

ACCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTTACTCT  >  NC_002947/5013187‑5013483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`