Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,026,798	A→T	7.9%	T20T (ACA→ACT)	ocd →	ornithine cyclodeaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,026,798	0	A	T	7.9%	277.8 / 9.0	89	T20T (ACA→ACT)	ocd	ornithine cyclodeaminase
Reads supporting (aligned to +/- strand):  ref base A (39/42);  new base T (3/4);  total (42/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGGAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAATTTCG  >  NC_002947/5026650‑5026943                                                                                                                                                      |                                                                                                                                                  ggTGGGAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATacag                                                                                                                                                  <  1:2254350/150‑1 (MQ=255)  gTGGGAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCTTATCGCTGGATacaga                                                                                                                                                 <  2:102066/150‑1 (MQ=255)  gTGGGAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATacaga                                                                                                                                                 >  2:504335/1‑150 (MQ=255)   tGGGAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATacagac                                                                                                                                                >  2:313952/1‑150 (MQ=255)           tCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGa                                                                                                                                        <  2:782637/150‑1 (MQ=255)           tCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGa                                                                                                                                        <  1:850627/150‑1 (MQ=255)            cGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTAAGTGCGTAACGCTCGATACTGTCTGTATCGag                                                                                                                                       <  1:1770418/150‑2 (MQ=255)             gACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCCTGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACTGTCTGTATCGagc                                                                                                                                      <  2:466334/150‑3 (MQ=255)             gACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCCTGAACTGTGAGTGCGTAACGCTCGATACAGACAGTATCGATg                                                                                                                                      >  1:1833705/1‑150 (MQ=255)              aCATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACTGTCTGTATCGa                                                                                                                                        >  2:442247/1‑147 (MQ=255)              aCATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACTGTCTGTATCGa                                                                                                                                        <  1:442247/147‑1 (MQ=255)              aCATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGt                                                                                                                                     >  2:1216503/1‑150 (MQ=255)                aTGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAg                                                                                                                                   <  1:2349034/150‑1 (MQ=255)                  ggCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAACACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTg                                                                                                                                 >  2:1073045/1‑150 (MQ=255)                   gCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTgg                                                                                                                                <  1:711712/150‑1 (MQ=255)                       aaCACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGaaaa                                                                                                                            <  1:504335/150‑1 (MQ=255)                          acCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACTGTCTGTATCGa                                                                                                                                        <  1:90394/135‑1 (MQ=255)                          acCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACTGTCTGTATCGa                                                                                                                                        >  2:90394/1‑135 (MQ=255)                            cAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCAt                                                                                                                       >  2:617286/1‑150 (MQ=255)                              aCGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATAAGTGAGTGCGTAACGCTCGATACAGACAGTATCGCTGTACTGGTTAACTCAttt                                                                                                                     <  2:703315/150‑1 (MQ=255)                                 catcatCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCa                                                                                                                  >  1:1664237/1‑150 (MQ=255)                                      tcCTGGAAGCTGTGCGCGACCGCCGCCCAACACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCt                                                                                                             <  1:1073045/150‑1 (MQ=255)                                       ccTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTg                                                                                                            <  1:236167/150‑1 (MQ=255)                                         tGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGc                                                                                                          <  1:1216503/150‑1 (MQ=255)                                             aGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCaga                                                                                                      <  1:2145521/150‑1 (MQ=255)                                              gCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCagag                                                                                                     <  2:2058041/150‑1 (MQ=255)                                              gCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCagag                                                                                                     <  1:313952/150‑1 (MQ=255)                                                tgtgCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTAt                                                                                                                                           <  1:1606887/110‑1 (MQ=255)                                                tgtgCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTAt                                                                                                                                           >  2:1606887/1‑110 (MQ=255)                                                tgtgCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTCAGCGAAGT‑CCTCACGCTAACCCAACACGACATTCTTCCGTTCTTAACGGTGGACACGGACAGTATCGTGGTAGTGTAAAACGCATTTGCATCGCTGGCCAGAGGc                                                                                                   <  2:274907/150‑1 (MQ=255)                                                       gACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTgg                                                                                            >  1:2075877/1‑150 (MQ=255)                                                         ccgccgCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTc                                                                                          <  2:801935/150‑1 (MQ=255)                                                         cagccgccCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGAAAAGTGGTc                                                                                          >  2:2337981/3‑150 (MQ=255)                                                           gccgccCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGt                                                                                              <  1:113598/144‑1 (MQ=255)                                                           gccgccCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGt                                                                                              >  2:113598/1‑144 (MQ=255)                                                              gccCCGCACGTGCTTAAAGAGGTGCCCTGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGcc                                                                                     >  2:2220216/1‑150 (MQ=255)                                                                ccAGCACGTGCTTAAAGATGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAAAGCGAGATCCGTGAGTGCGCAACGCGCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGt                                                                                   >  1:1499198/1‑150 (MQ=255)                                                                ccAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTGACGCGCGATacaga                                                                                                                                                 >  1:2098120/1‑88 (MQ=255)                                                                ccAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATacaga                                                                                                                                                 <  2:2098120/88‑1 (MQ=255)                                                                   gCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAGCGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCaa                                                                                >  2:596051/1‑150 (MQ=255)                                                                       gTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAAtcct                                                                            >  2:1013120/1‑150 (MQ=255)                                                                       gTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAAtcct                                                                            >  2:112338/1‑150 (MQ=255)                                                                         gCTTAAAGAGGTGCCATGTAAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAAAGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAAtactcc                                                                          >  2:495151/1‑150 (MQ=255)                                                                         gCTTAAAGAGGTGCAATGTGAGCGAAGT‑CCTTCTGCTTAACCACCGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAAtcctcc                                                                          <  1:951607/150‑1 (MQ=255)                                                                          cTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCg                                                                         <  1:617286/150‑1 (MQ=255)                                                                           ttAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATATTGACAGTATCGATGTAGTGGGAAACCCATTTGCATCGCTGGCCAGACGCACAGTGGTCATGCCGTCAATCCTCCgg                                                                        >  2:1389823/1‑150 (MQ=255)                                                                           ttAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCgg                                                                        <  1:112338/150‑1 (MQ=255)                                                                            tAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAAACAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGt                                                                       >  1:440850/1‑150 (MQ=255)                                                                              aaGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTa                                                                     >  1:2067544/1‑150 (MQ=255)                                                                              aaGAGGTGCCATGTGA‑CCAAGTGTTTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTa                                                                     <  1:1328582/150‑1 (MQ=255)                                                                                      ccATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCaa                                                                                <  2:1974836/131‑1 (MQ=255)                                                                                      ccATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCaa                                                                                >  1:1974836/1‑131 (MQ=255)                                                                                         tgtgAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGa                                                          <  2:1664237/150‑1 (MQ=255)                                                                                         tgtgAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGa                                                          >  1:524864/1‑150 (MQ=255)                                                                                         tgtgAGCGAAGT‑CCTTCTGCTTAAACAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGa                                                          <  2:440850/150‑1 (MQ=255)                                                                                          gtgAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCaga                                                                                                      >  2:1122437/1‑105 (MQ=255)                                                                                          gtgAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCaga                                                                                                      <  1:1122437/105‑1 (MQ=255)                                                                                           tgAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGc                                                        >  2:315221/1‑150 (MQ=255)                                                                                              gCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCg                                                                         <  2:1909792/130‑1 (MQ=255)                                                                                              gCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCg                                                                         >  1:1909792/1‑130 (MQ=255)                                                                                               cGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACaa                                                    <  2:1499198/150‑1 (MQ=255)                                                                                                   gT‑CCTTCTTCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGt                                                                                   <  1:1047241/115‑1 (MQ=255)                                                                                                   gT‑CCTTCTTCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGt                                                                                   >  2:1047241/1‑115 (MQ=255)                                                                                                    t‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGtt                                                                      >  1:835236/1‑127 (MQ=255)                                                                                                    t‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGtt                                                                      <  2:835236/127‑1 (MQ=255)                                                                                                    t‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGa                                                                   <  1:734339/130‑1 (MQ=255)                                                                                                    t‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGa                                                                   >  2:734339/1‑130 (MQ=255)                                                                                                          cTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTg                                          <  2:524864/150‑1 (MQ=255)                                                                                                           tGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTACGAGCACAATGGCGAGGTgg                                         >  2:1097213/1‑150 (MQ=255)                                                                                                            gCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGa                                        <  2:1277340/150‑1 (MQ=255)                                                                                                             cTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTa                                                                     <  2:206520/120‑1 (MQ=255)                                                                                                             cTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTa                                                                     >  1:206520/1‑120 (MQ=255)                                                                                                             cTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGAc                                       <  2:2067544/150‑1 (MQ=255)                                                                                                             cTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGAc                                       <  1:596051/150‑1 (MQ=255)                                                                                                                  ccAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAg                                                                                               >  1:109339/1‑89 (MQ=255)                                                                                                                  ccAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAg                                                                                               <  2:109339/89‑1 (MQ=255)                                                                                                                   cAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCaaa                                 >  2:816877/1‑150 (MQ=255)                                                                                                                   cAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCaaa                                 >  2:1349386/1‑150 (MQ=255)                                                                                                                    aaCGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCaaaa                                >  1:1579918/1‑150 (MQ=255)                                                                                                                         gAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCt                           <  1:1013120/150‑1 (MQ=255)                                                                                                                                gTGAGTGCGTAACGGTCAAGACAGACAGTATCGATGTAGTGGAAAACGCGTTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGATTACCTGc                    >  1:695122/1‑150 (MQ=255)                                                                                                                                gTGAGTGCATAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGc                    <  1:816877/150‑1 (MQ=255)                                                                                                                                   aGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGt                                                                                   >  2:1486977/1‑84 (MQ=255)                                                                                                                                   aGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGt                                                                                   <  1:1486977/84‑1 (MQ=255)                                                                                                                                        gTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCtt            >  2:1608634/1‑150 (MQ=255)                                                                                                                                        gTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCtt            >  2:1818040/1‑150 (MQ=255)                                                                                                                                        gTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCAAACTTGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCtt            >  1:634691/1‑150 (MQ=255)                                                                                                                                          aaCGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTga          <  1:1349386/150‑1 (MQ=255)                                                                                                                                               tCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCaa                                                                                >  1:1840567/1‑75 (MQ=255)                                                                                                                                               tCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCaa                                                                                <  2:1840567/75‑1 (MQ=255)                                                                                                                                               tCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGCTCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAAtt     >  1:658982/1‑150 (MQ=255)                                                                                                                                                 gctACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGCCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAACAAGGCGTACCTGCCAGACCACGGGAATTTc   >  1:506680/3‑150 (MQ=255)                                                                                                                                                 gATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAATTTc   <  2:1579918/150‑1 (MQ=255)                                                                                                                                                  aTACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAATTTCg  <  2:2075877/150‑1 (MQ=255)                                                                                                                                                  aTACACACAGTACCGATGTAGTGGAAAACGCATTTCCATCGCTGGCCAGAGGCAAAGTGCTCATGTCGCCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGCACGTCAAAACCGCTTACATGCCAGACCTAGAGACTTTCg  <  2:506680/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  GGTGGGAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGT‑CCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAATTTCG  >  NC_002947/5026650‑5026943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑