Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,205,866 C→G 6.9% R205R (CGG→CGC)  PP_4586 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,205,8660CG 6.9% 230.3 / 7.4 74R205R (CGG→CGC) PP_4586hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (43/25);  new base G (3/2);  total (47/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

TGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGA  >  NC_002947/5205718‑5206014
                                                                                                                                                    |                                                                                                                                                    
tGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGcgcc                                                                                                                                                     >  2:426711/1‑150 (MQ=255)
       gTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCAtgcc                                                                                                                                              >  2:1061659/1‑150 (MQ=255)
       gTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCAtgcc                                                                                                                                              >  2:293867/1‑150 (MQ=255)
             cGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCGGCAtgccctggcc                                                                                                                                        >  1:2218879/1‑150 (MQ=255)
                             aTAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                        <  1:2054881/150‑1 (MQ=255)
                             aTAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                        >  1:1863083/1‑150 (MQ=255)
                                 ccccTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCa                                                                                                                    >  2:235415/1‑150 (MQ=255)
                                    cTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                        >  1:2186922/1‑143 (MQ=255)
                                    cTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                        <  2:2186922/143‑1 (MQ=255)
                                    cTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACtt                                                                                                                 >  2:562640/1‑150 (MQ=255)
                                    cTCGCCACATGCCGTCACGCACCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACtt                                                                                                                 >  2:990697/1‑150 (MQ=255)
                                           caTGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGtctc                                                                                                          <  2:2285225/150‑1 (MQ=255)
                                                 gTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAg                                                                                                    >  2:2325594/1‑150 (MQ=255)
                                                      gcgcCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCAcc                                                                                                                                    >  2:1595251/1‑113 (MQ=255)
                                                      gcgcCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCAcc                                                                                                                                    <  1:1595251/113‑1 (MQ=255)
                                                      gcgcCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAg                                                                                               <  1:293867/150‑1 (MQ=255)
                                                                cAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCt                                                                                     >  2:854176/1‑150 (MQ=255)
                                                                  agagCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGc                                                                                   >  1:501964/1‑150 (MQ=255)
                                                                   gagCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGcc                                                                                  <  2:679105/150‑1 (MQ=255)
                                                                   gagCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCATCTCAGTCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGcc                                                                                  >  1:1354884/1‑150 (MQ=255)
                                                                      cagcTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCTCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAg                                                                               <  2:1182090/150‑1 (MQ=255)
                                                                        gcTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCATACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGt                                                                             >  1:305449/1‑150 (MQ=255)
                                                                             ggCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCg                                                                        >  1:2027697/1‑150 (MQ=255)
                                                                              gCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCgg                                                                       >  1:1997532/1‑150 (MQ=255)
                                                                              gCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCgg                                                                       >  1:697694/1‑150 (MQ=255)
                                                                                gggTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGcc                                                                     >  2:469734/1‑149 (MQ=255)
                                                                                 ggTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCaa                                                                    <  1:1566577/150‑1 (MQ=255)
                                                                                    tACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCa                                                                 >  2:1114885/1‑150 (MQ=255)
                                                                                        cgGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCa                                                             >  2:1502145/1‑150 (MQ=255)
                                                                                         gggAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAg                                                            <  1:426711/150‑1 (MQ=255)
                                                                                               acaGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTc                                                      >  1:2144053/1‑150 (MQ=255)
                                                                                               acaGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTc                                                      >  2:1536661/1‑150 (MQ=255)
                                                                                                caGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCg                                                     <  1:1061659/150‑1 (MQ=255)
                                                                                                caGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGAGGTGTCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCg                                                     >  1:1496495/1‑150 (MQ=255)
                                                                                                caGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCACGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCg                                                     <  1:1071532/150‑1 (MQ=255)
                                                                                                 aGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCgg                                                    <  1:785534/150‑1 (MQ=255)
                                                                                                   cTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGc                                                  >  2:556932/1‑150 (MQ=255)
                                                                                                       tAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATc                                              <  2:858828/150‑1 (MQ=255)
                                                                                                          ttGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                           <  2:1997532/150‑1 (MQ=255)
                                                                                                          ttGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                           >  1:2081628/1‑150 (MQ=255)
                                                                                                              ggCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAgg                                       <  1:235415/150‑1 (MQ=255)
                                                                                                              ggCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAgg                                       <  1:990697/150‑1 (MQ=255)
                                                                                                               gCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCTCTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGgc                                      >  1:170271/1‑150 (MQ=255)
                                                                                                                 cAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGgcgc                                    <  2:670518/150‑1 (MQ=255)
                                                                                                                 cAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGATGCCCAGGCCAAAGGCAAGCATCCAGCCGGTCTGGCGATCGAACAGgcgc                                    >  2:1831347/1‑150 (MQ=255)
                                                                                                                   gACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGa                                  >  1:1860940/1‑150 (MQ=255)
                                                                                                                    aCGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGac                                 >  1:1929365/1‑150 (MQ=255)
                                                                                                                      gAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGacac                               >  1:2366519/1‑150 (MQ=255)
                                                                                                                        aTGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGt                             >  2:116977/1‑150 (MQ=255)
                                                                                                                         tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGc                                                                                                  <  1:684766/80‑1 (MQ=255)
                                                                                                                         tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGc                                                                                                  >  2:684766/1‑80 (MQ=255)
                                                                                                                         tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGtt                            <  2:2366519/150‑1 (MQ=255)
                                                                                                                         tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGtt                            >  1:1574658/1‑150 (MQ=255)
                                                                                                                         tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGtt                            >  1:98447/1‑150 (MQ=255)
                                                                                                                              gCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGa                       >  1:1297370/1‑150 (MQ=255)
                                                                                                                                  ggTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCg                   >  2:1928708/1‑150 (MQ=255)
                                                                                                                                  ggTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCg                   >  2:2213336/1‑150 (MQ=255)
                                                                                                                                   gTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCaa                                                                    <  1:1059772/100‑1 (MQ=255)
                                                                                                                                   gTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCaa                                                                    >  2:1059772/1‑100 (MQ=255)
                                                                                                                                     cAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGaaa                >  2:909986/1‑150 (MQ=255)
                                                                                                                                     cAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGaaa                <  1:1928708/150‑1 (MQ=255)
                                                                                                                                     cAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGaaa                <  1:1114885/150‑1 (MQ=255)
                                                                                                                                      aGGCTGTCCATGCGCGGCATGCCCTGCCCCACCGGCGCGGCCACCGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACCCGTTGTCGATGCGAGAc               >  2:301606/1‑150 (MQ=255)
                                                                                                                                      aGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAgg                                                                              >  1:1209133/1‑87 (MQ=255)
                                                                                                                                      aGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAgg                                                                              <  2:1209133/87‑1 (MQ=255)
                                                                                                                                      aGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAAc               >  1:2156449/1‑150 (MQ=255)
                                                                                                                                        gcagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                           <  2:1257787/114‑1 (MQ=255)
                                                                                                                                        gcagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                           >  1:1257787/7‑120 (MQ=255)
                                                                                                                                        gcagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACtt             >  1:1077522/7‑150 (MQ=255)
                                                                                                                                         cagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCCCGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGc                    <  2:2077975/137‑1 (MQ=255)
                                                                                                                                         cagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGc                    >  1:2077975/6‑142 (MQ=255)
                                                                                                                                          tGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTcc           <  1:909986/150‑1 (MQ=255)
                                                                                                                                           gTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTccc          <  1:2325594/150‑1 (MQ=255)
                                                                                                                                               aTGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTg      >  2:94484/1‑150 (MQ=255)
                                                                                                                                                tGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGc     >  2:2141388/1‑150 (MQ=255)
                                                                                                                                                   gccgcATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGa  >  1:888385/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGA  >  NC_002947/5205718‑5206014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑