Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,205,874 C→A 7.0% G203C (GGC→TGC) ‡ PP_4586 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,205,8740CA 7.0% 222.4 / 5.2 72G203C (GGC→TGC) ‡PP_4586hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (42/24);  new base A (3/2);  total (45/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

GTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGCC  >  NC_002947/5205725‑5206023
                                                                                                                                                     |                                                                                                                                                     
gTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCAtgcc                                                                                                                                                       >  2:1061659/1‑150 (MQ=255)
gTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCAtgcc                                                                                                                                                       >  2:293867/1‑150 (MQ=255)
      cGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCGGCAtgccctggcc                                                                                                                                                 >  1:2218879/1‑150 (MQ=255)
                      aTAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                                 <  1:2054881/150‑1 (MQ=255)
                      aTAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                                 >  1:1863083/1‑150 (MQ=255)
                          ccccTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCa                                                                                                                             >  2:235415/1‑150 (MQ=255)
                             cTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                                 >  1:2186922/1‑143 (MQ=255)
                             cTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                                 <  2:2186922/143‑1 (MQ=255)
                             cTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACtt                                                                                                                          >  2:562640/1‑150 (MQ=255)
                             cTCGCCACATGCCGTCACGCACCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACtt                                                                                                                          >  2:990697/1‑150 (MQ=255)
                                    caTGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGtctc                                                                                                                   <  2:2285225/150‑1 (MQ=255)
                                          gTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAg                                                                                                             >  2:2325594/1‑150 (MQ=255)
                                               gcgcCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCAcc                                                                                                                                             <  1:1595251/113‑1 (MQ=255)
                                               gcgcCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCAcc                                                                                                                                             >  2:1595251/1‑113 (MQ=255)
                                               gcgcCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAg                                                                                                        <  1:293867/150‑1 (MQ=255)
                                                         cAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCt                                                                                              >  2:854176/1‑150 (MQ=255)
                                                           agagCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGc                                                                                            >  1:501964/1‑150 (MQ=255)
                                                            gagCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGcc                                                                                           <  2:679105/150‑1 (MQ=255)
                                                            gagCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCATCTCAGTCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGcc                                                                                           >  1:1354884/1‑150 (MQ=255)
                                                               cagcTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCTCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAg                                                                                        <  2:1182090/150‑1 (MQ=255)
                                                                 gcTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCATACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGt                                                                                      >  1:305449/1‑150 (MQ=255)
                                                                      ggCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCg                                                                                 >  1:2027697/1‑150 (MQ=255)
                                                                       gCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCgg                                                                                >  1:1997532/1‑150 (MQ=255)
                                                                       gCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCgg                                                                                >  1:697694/1‑150 (MQ=255)
                                                                         gggTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGcc                                                                              >  2:469734/1‑149 (MQ=255)
                                                                          ggTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCaa                                                                             <  1:1566577/150‑1 (MQ=255)
                                                                             tACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCa                                                                          >  2:1114885/1‑150 (MQ=255)
                                                                                 cgGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCa                                                                      >  2:1502145/1‑150 (MQ=255)
                                                                                  gggAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAg                                                                     <  1:426711/150‑1 (MQ=255)
                                                                                        acaGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTc                                                               >  1:2144053/1‑150 (MQ=255)
                                                                                        acaGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTc                                                               >  2:1536661/1‑150 (MQ=255)
                                                                                         caGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCg                                                              <  1:1061659/150‑1 (MQ=255)
                                                                                         caGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGAGGTGTCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCg                                                              >  1:1496495/1‑150 (MQ=255)
                                                                                         caGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCACGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCg                                                              <  1:1071532/150‑1 (MQ=255)
                                                                                          aGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCgg                                                             <  1:785534/150‑1 (MQ=255)
                                                                                            cTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGc                                                           >  2:556932/1‑150 (MQ=255)
                                                                                                tAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATc                                                       <  2:858828/150‑1 (MQ=255)
                                                                                                   ttGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                                    <  2:1997532/150‑1 (MQ=255)
                                                                                                   ttGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                                    >  1:2081628/1‑150 (MQ=255)
                                                                                                       ggCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAgg                                                <  1:235415/150‑1 (MQ=255)
                                                                                                       ggCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAgg                                                <  1:990697/150‑1 (MQ=255)
                                                                                                        gCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCTCTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGgc                                               >  1:170271/1‑150 (MQ=255)
                                                                                                          cAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGgcgc                                             <  2:670518/150‑1 (MQ=255)
                                                                                                          cAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGATGCCCAGGCCAAAGGCAAGCATCCAGCCGGTCTGGCGATCGAACAGgcgc                                             >  2:1831347/1‑150 (MQ=255)
                                                                                                            gACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGa                                           >  1:1860940/1‑150 (MQ=255)
                                                                                                             aCGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGac                                          >  1:1929365/1‑150 (MQ=255)
                                                                                                               gAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGacac                                        >  1:2366519/1‑150 (MQ=255)
                                                                                                                 aTGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGt                                      >  2:116977/1‑150 (MQ=255)
                                                                                                                  tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGc                                                                                                           >  2:684766/1‑80 (MQ=255)
                                                                                                                  tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGc                                                                                                           <  1:684766/80‑1 (MQ=255)
                                                                                                                  tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGtt                                     >  1:98447/1‑150 (MQ=255)
                                                                                                                  tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGtt                                     <  2:2366519/150‑1 (MQ=255)
                                                                                                                  tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGtt                                     >  1:1574658/1‑150 (MQ=255)
                                                                                                                       gCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGa                                >  1:1297370/1‑150 (MQ=255)
                                                                                                                           ggTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCg                            >  2:1928708/1‑150 (MQ=255)
                                                                                                                           ggTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCg                            >  2:2213336/1‑150 (MQ=255)
                                                                                                                            gTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCaa                                                                             <  1:1059772/100‑1 (MQ=255)
                                                                                                                            gTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCaa                                                                             >  2:1059772/1‑100 (MQ=255)
                                                                                                                              cAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGaaa                         <  1:1114885/150‑1 (MQ=255)
                                                                                                                              cAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGaaa                         >  2:909986/1‑150 (MQ=255)
                                                                                                                              cAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGaaa                         <  1:1928708/150‑1 (MQ=255)
                                                                                                                               aGGCTGTCCATGCGCGGCATGCCCTGCCCCACCGGCGCGGCCACCGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACCCGTTGTCGATGCGAGAc                        >  2:301606/1‑150 (MQ=255)
                                                                                                                               aGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAgg                                                                                       >  1:1209133/1‑87 (MQ=255)
                                                                                                                               aGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAgg                                                                                       <  2:1209133/87‑1 (MQ=255)
                                                                                                                               aGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAAc                        >  1:2156449/1‑150 (MQ=255)
                                                                                                                                 gcagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                                    <  2:1257787/114‑1 (MQ=255)
                                                                                                                                 gcagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                                    >  1:1257787/7‑120 (MQ=255)
                                                                                                                                 gcagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACtt                      >  1:1077522/7‑150 (MQ=255)
                                                                                                                                  cagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCCCGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGc                             <  2:2077975/137‑1 (MQ=255)
                                                                                                                                  cagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGc                             >  1:2077975/6‑142 (MQ=255)
                                                                                                                                   tGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTcc                    <  1:909986/150‑1 (MQ=255)
                                                                                                                                    gTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTccc                   <  1:2325594/150‑1 (MQ=255)
                                                                                                                                        aTGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTg               >  2:94484/1‑150 (MQ=255)
                                                                                                                                         tGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGc              >  2:2141388/1‑150 (MQ=255)
                                                                                                                                            gccgcATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGa           >  1:888385/1‑150 (MQ=255)
                                                                                                                                                  tgccctgcccCACCGGCGCGGCCACGGTCATTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGcgcg     <  2:1461180/150‑1 (MQ=255)
                                                                                                                                                     cctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGcc  >  2:608144/1‑150 (MQ=255)
                                                                                                                                                     cctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGcc  >  1:642936/1‑150 (MQ=255)
                                                                                                                                                     cctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGcc  >  2:967816/1‑150 (MQ=255)
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GTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGCC  >  NC_002947/5205725‑5206023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑