Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,205,876 T→A 6.9% Q202L (CAG→CTG)  PP_4586 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,205,8760TA 6.9% 227.0 / 6.7 72Q202L (CAG→CTG) PP_4586hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (42/25);  new base A (3/2);  total (45/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

CGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGCCA  >  NC_002947/5205731‑5206024
                                                                                                                                                 |                                                                                                                                                    
cGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCGGCAtgccctggcc                                                                                                                                                  >  1:2218879/1‑150 (MQ=255)
                aTAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                                  <  1:2054881/150‑1 (MQ=255)
                aTAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                                  >  1:1863083/1‑150 (MQ=255)
                    ccccTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCa                                                                                                                              >  2:235415/1‑150 (MQ=255)
                       cTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                                  >  1:2186922/1‑143 (MQ=255)
                       cTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACg                                                                                                                                  <  2:2186922/143‑1 (MQ=255)
                       cTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACtt                                                                                                                           >  2:562640/1‑150 (MQ=255)
                       cTCGCCACATGCCGTCACGCACCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACtt                                                                                                                           >  2:990697/1‑150 (MQ=255)
                              caTGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGtctc                                                                                                                    <  2:2285225/150‑1 (MQ=255)
                                    gTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAg                                                                                                              >  2:2325594/1‑150 (MQ=255)
                                         gcgcCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCAcc                                                                                                                                              >  2:1595251/1‑113 (MQ=255)
                                         gcgcCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCAcc                                                                                                                                              <  1:1595251/113‑1 (MQ=255)
                                         gcgcCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAg                                                                                                         <  1:293867/150‑1 (MQ=255)
                                                   cAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCt                                                                                               >  2:854176/1‑150 (MQ=255)
                                                     agagCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGc                                                                                             >  1:501964/1‑150 (MQ=255)
                                                      gagCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGcc                                                                                            <  2:679105/150‑1 (MQ=255)
                                                      gagCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCATCTCAGTCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGcc                                                                                            >  1:1354884/1‑150 (MQ=255)
                                                         cagcTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCTCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAg                                                                                         <  2:1182090/150‑1 (MQ=255)
                                                           gcTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCATACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGt                                                                                       >  1:305449/1‑150 (MQ=255)
                                                                ggCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCg                                                                                  >  1:2027697/1‑150 (MQ=255)
                                                                 gCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCgg                                                                                 >  1:697694/1‑150 (MQ=255)
                                                                 gCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCgg                                                                                 >  1:1997532/1‑150 (MQ=255)
                                                                   gggTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGcc                                                                               >  2:469734/1‑149 (MQ=255)
                                                                    ggTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCaa                                                                              <  1:1566577/150‑1 (MQ=255)
                                                                       tACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCa                                                                           >  2:1114885/1‑150 (MQ=255)
                                                                           cgGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCa                                                                       >  2:1502145/1‑150 (MQ=255)
                                                                            gggAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAg                                                                      <  1:426711/150‑1 (MQ=255)
                                                                                  acaGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTc                                                                >  1:2144053/1‑150 (MQ=255)
                                                                                  acaGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTc                                                                >  2:1536661/1‑150 (MQ=255)
                                                                                   caGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCg                                                               <  1:1061659/150‑1 (MQ=255)
                                                                                   caGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGAGGTGTCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCg                                                               >  1:1496495/1‑150 (MQ=255)
                                                                                   caGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCACGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCg                                                               <  1:1071532/150‑1 (MQ=255)
                                                                                    aGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCgg                                                              <  1:785534/150‑1 (MQ=255)
                                                                                      cTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGc                                                            >  2:556932/1‑150 (MQ=255)
                                                                                          tAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATc                                                        <  2:858828/150‑1 (MQ=255)
                                                                                             ttGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                                     >  1:2081628/1‑150 (MQ=255)
                                                                                             ttGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                                     <  2:1997532/150‑1 (MQ=255)
                                                                                                 ggCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAgg                                                 <  1:235415/150‑1 (MQ=255)
                                                                                                 ggCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAgg                                                 <  1:990697/150‑1 (MQ=255)
                                                                                                  gCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCTCTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGgc                                                >  1:170271/1‑150 (MQ=255)
                                                                                                    cAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGgcgc                                              <  2:670518/150‑1 (MQ=255)
                                                                                                    cAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGATGCCCAGGCCAAAGGCAAGCATCCAGCCGGTCTGGCGATCGAACAGgcgc                                              >  2:1831347/1‑150 (MQ=255)
                                                                                                      gACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGa                                            >  1:1860940/1‑150 (MQ=255)
                                                                                                       aCGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGac                                           >  1:1929365/1‑150 (MQ=255)
                                                                                                         gAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGacac                                         >  1:2366519/1‑150 (MQ=255)
                                                                                                           aTGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGt                                       >  2:116977/1‑150 (MQ=255)
                                                                                                            tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGc                                                                                                            >  2:684766/1‑80 (MQ=255)
                                                                                                            tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGc                                                                                                            <  1:684766/80‑1 (MQ=255)
                                                                                                            tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGtt                                      <  2:2366519/150‑1 (MQ=255)
                                                                                                            tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGtt                                      >  1:98447/1‑150 (MQ=255)
                                                                                                            tgtgAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGtt                                      >  1:1574658/1‑150 (MQ=255)
                                                                                                                 gCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGa                                 >  1:1297370/1‑150 (MQ=255)
                                                                                                                     ggTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCg                             >  2:2213336/1‑150 (MQ=255)
                                                                                                                     ggTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCg                             >  2:1928708/1‑150 (MQ=255)
                                                                                                                      gTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCaa                                                                              <  1:1059772/100‑1 (MQ=255)
                                                                                                                      gTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCaa                                                                              >  2:1059772/1‑100 (MQ=255)
                                                                                                                        cAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGaaa                          <  1:1928708/150‑1 (MQ=255)
                                                                                                                        cAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGaaa                          >  2:909986/1‑150 (MQ=255)
                                                                                                                        cAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGaaa                          <  1:1114885/150‑1 (MQ=255)
                                                                                                                         aGGCTGTCCATGCGCGGCATGCCCTGCCCCACCGGCGCGGCCACCGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACCCGTTGTCGATGCGAGAc                         >  2:301606/1‑150 (MQ=255)
                                                                                                                         aGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAgg                                                                                        >  1:1209133/1‑87 (MQ=255)
                                                                                                                         aGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAgg                                                                                        <  2:1209133/87‑1 (MQ=255)
                                                                                                                         aGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAAc                         >  1:2156449/1‑150 (MQ=255)
                                                                                                                           gcagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                                     <  2:1257787/114‑1 (MQ=255)
                                                                                                                           gcagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGaa                                                     >  1:1257787/7‑120 (MQ=255)
                                                                                                                           gcagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACtt                       >  1:1077522/7‑150 (MQ=255)
                                                                                                                            cagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCCCGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGc                              <  2:2077975/137‑1 (MQ=255)
                                                                                                                            cagggcATGCGGCGCATGGACAGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGc                              >  1:2077975/6‑142 (MQ=255)
                                                                                                                             tGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTcc                     <  1:909986/150‑1 (MQ=255)
                                                                                                                              gTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTccc                    <  1:2325594/150‑1 (MQ=255)
                                                                                                                                  aTGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTg                >  2:94484/1‑150 (MQ=255)
                                                                                                                                   tGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGc               >  2:2141388/1‑150 (MQ=255)
                                                                                                                                      gccgcATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGa            >  1:888385/1‑150 (MQ=255)
                                                                                                                                            tgccctgcccCACCGGCGCGGCCACGGTCATTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGcgcg      <  2:1461180/150‑1 (MQ=255)
                                                                                                                                               cctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGcc   >  1:642936/1‑150 (MQ=255)
                                                                                                                                               cctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGcc   >  2:608144/1‑150 (MQ=255)
                                                                                                                                               cctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGcc   >  2:967816/1‑150 (MQ=255)
                                                                                                                                                ctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTg                <  1:1525668/136‑1 (MQ=255)
                                                                                                                                                ctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTg                <  1:1262658/136‑1 (MQ=255)
                                                                                                                                                ctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTg                >  2:1525668/1‑136 (MQ=255)
                                                                                                                                                ctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTg                >  2:1262658/1‑136 (MQ=255)
                                                                                                                                                ctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGCCa  <  2:305449/150‑1 (MQ=255)
                                                                                                                                                ctgcccCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGCCa  >  2:628313/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAACACAGCTTGTAGTTGTGGCCAGACGAATGTGAGCAAGGTCAGGCTGTCCATGCGCCGCATGCCCTGCCCCACCGGCGCGGCCACGGTCACTTCGGTCTCGCCCAGGCGAGCGCGGCGGCTGCCCAGGTCGCCGGCAAGCATCCAGCCGGTCGGGCGATCGAACAGGCGCGACACGTTGTCGATGCGAAACTTCCCCTTGCCGATGCGCGGCCA  >  NC_002947/5205731‑5206024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑