Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,208,730 G→A 5.5% D9D (GAC→GAT) ‡ PP_4589 ← D‑isomer specific 2‑hydroxyacid dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,208,7300GA 5.5% 392.0 / 8.7 128D9D (GAC→GAT) ‡PP_4589D‑isomer specific 2‑hydroxyacid dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (62/59);  new base A (5/2);  total (67/61)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01

Only 100 of 131 total aligned reads displayed.

CCTTGGCGCAGCAGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCTTGGTGA  >  NC_002947/5208581‑5208877
                                                                                                                                                     |                                                                                                                                                   
ccTTGGCGCAGCAGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATgg                                                                                                                                                     >  1:1060786/1‑150 (MQ=255)
 cTTGGCGCAGCAGACTGGCCAGCAAGTCGCGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGt                                                                                                                                                    >  1:676477/1‑150 (MQ=255)
 cTTGGCGCAGCAGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGt                                                                                                                                                    >  1:694501/1‑150 (MQ=255)
    ggCGCAGCAGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCAt                                                                                                                                                 <  1:2193981/150‑1 (MQ=255)
          gcagACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCACGGCGTATCGCGCCTGGTCCTGCTccg                                                                                                                                           >  2:509127/1‑148 (MQ=255)
           cagACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGc                                                                                                                                          >  2:1465767/1‑150 (MQ=255)
                tGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCa                                                                                                                                     >  1:1436651/1‑150 (MQ=255)
                  gCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGt                                                                                                                                   >  2:2205439/1‑150 (MQ=255)
                  gCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGt                                                                                                                                   >  2:38973/1‑150 (MQ=255)
                   ccAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCACGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTa                                                                                                                                  >  1:2068407/1‑150 (MQ=255)
                    cAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTAc                                                                                                                                 >  1:579264/1‑150 (MQ=255)
                          gTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCAt                                                                                                                           >  1:2019155/1‑150 (MQ=255)
                           tCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATg                                                                                                                          <  1:251279/150‑1 (MQ=255)
                           tCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAACGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATg                                                                                                                          >  1:1968572/1‑150 (MQ=255)
                            ccGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATgg                                                                                                                         <  2:1961337/150‑1 (MQ=255)
                             cGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGt                                                                                                                        <  1:474711/150‑1 (MQ=255)
                              ggCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTc                                                                                                                       >  1:1677753/1‑150 (MQ=255)
                                    cccAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACgg                                                                                                                 >  2:1219518/1‑150 (MQ=255)
                                     ccAGCCACCCCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACggg                                                                                                                <  1:694134/150‑1 (MQ=255)
                                     ccAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGAAGAGATTCTTCGCGGCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACggg                                                                                                                <  2:676477/150‑1 (MQ=255)
                                      cAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACgggg                                                                                                               >  1:121741/1‑150 (MQ=255)
                                         ccACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAATCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCg                                                                                                            >  2:554839/1‑150 (MQ=255)
                                           acacCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGtt                                                                                                          >  1:1765466/1‑150 (MQ=255)
                                            cacCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACgg                                                                                                                 >  2:1357605/1‑142 (MQ=255)
                                            cacCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACgg                                                                                                                 <  1:1357605/142‑1 (MQ=255)
                                             acCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATgg                                                                                                                                                     <  1:1974302/105‑1 (MQ=255)
                                                 ggCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTgcggcg                                                                                                    <  2:776433/150‑1 (MQ=255)
                                                     cTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCgcagc                                                                                                <  2:1419847/150‑1 (MQ=255)
                                                      tGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCgcagca                                                                                               <  2:1917334/150‑1 (MQ=255)
                                                            gCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCtt                                                                                         <  2:1266043/150‑1 (MQ=255)
                                                              cTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCg                                                                                       <  2:2141124/150‑1 (MQ=255)
                                                              cTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCAAGTTGAGTCGTAGCTCCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCg                                                                                       <  2:63898/150‑1 (MQ=255)
                                                                   gggCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAg                                                                                  <  2:694501/150‑1 (MQ=255)
                                                                   gggCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAg                                                                                  <  1:2363708/150‑1 (MQ=255)
                                                                   gggCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAg                                                                                  >  2:1107499/1‑150 (MQ=255)
                                                                    ggCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAgg                                                                                 >  2:2406844/1‑150 (MQ=255)
                                                                       cAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGc                                                                              >  2:1960940/1‑150 (MQ=255)
                                                                        aGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCt                                                                             >  1:1044807/1‑150 (MQ=255)
                                                                          ttCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCg                                                                           >  2:1004177/1‑150 (MQ=255)
                                                                            cGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGAt                                                                         <  2:2311348/150‑1 (MQ=255)
                                                                            cGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGAt                                                                         <  2:1968572/150‑1 (MQ=255)
                                                                               cAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTa                                                                      >  1:1480972/1‑150 (MQ=255)
                                                                                agagTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGaagagc                                                                     <  2:579264/150‑6 (MQ=255)
                                                                                aaagTCGCCGCTGTTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGACGTAc                                                                     <  1:647632/148‑1 (MQ=255)
                                                                                aaagTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGAGCGGCGTATCGAGCATGGTCATGCTCAGCAATCAGTACACGCACCGTCAGACGGGGTCGTTGCGGCCCAGCAGCTCTTCGGCCAGGTGCTCGATGtcc                                                                     >  2:1103450/3‑148 (MQ=255)
                                                                                  agTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTc                                                                   <  2:2046523/150‑1 (MQ=255)
                                                                                   gTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCa                                                                  <  1:1436715/150‑1 (MQ=255)
                                                                                    tCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGc                                                                                                                                          >  1:1563924/1‑77 (MQ=255)
                                                                                    tCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCAt                                                                 >  2:1660320/1‑150 (MQ=255)
                                                                                    tCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCAt                                                                 <  1:226004/150‑1 (MQ=255)
                                                                                      gccgcTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCt                                                               >  2:1254286/1‑150 (MQ=255)
                                                                                       tcgcTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCtt                                                              >  2:1867576/2‑150 (MQ=255)
                                                                                          cTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCa                                                           <  2:313359/150‑1 (MQ=255)
                                                                                                 gCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGcggcgg                                                    >  1:845377/1‑150 (MQ=255)
                                                                                                  cACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCggcggc                                                   >  2:837084/1‑150 (MQ=255)
                                                                                                      tCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTa                                                                      >  2:2174088/1‑127 (MQ=255)
                                                                                                      tCCAGTTCTAGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCt                                               >  1:74934/1‑150 (MQ=255)
                                                                                                       ccAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCCGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTg                                              <  1:819057/150‑1 (MQ=255)
                                                                                                        cAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGc                                             >  1:1394438/1‑150 (MQ=255)
                                                                                                        cAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGc                                             >  1:2278008/1‑150 (MQ=255)
                                                                                                         aGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCa                                            >  1:525632/1‑150 (MQ=255)
                                                                                                          gtgcTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAg                                           >  2:2213690/4‑150 (MQ=255)
                                                                                                          gTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAg                                           >  2:408447/1‑150 (MQ=255)
                                                                                                           ttCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGCGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAgg                                          <  1:509127/150‑1 (MQ=255)
                                                                                                           ttCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAgg                                          <  1:1960940/150‑1 (MQ=255)
                                                                                                             cTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAggtg                                        >  1:566253/1‑150 (MQ=255)
                                                                                                              tGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGAt                                                                         <  1:186044/116‑1 (MQ=255)
                                                                                                              tGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGAt                                                                         >  2:186044/1‑116 (MQ=255)
                                                                                                                  gcggcggcgCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGc                                   <  1:1101580/150‑1 (MQ=255)
                                                                                                                   cggcggcgCGCAAACGCGCGGCGTATCGAGCATGGTCATGCTCAGCGAGCAGTACGTGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTTGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGcc                                  <  2:181601/150‑1 (MQ=255)
                                                                                                                   cggcggcgCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAggtggtcgag                                  <  2:1179751/150‑5 (MQ=255)
                                                                                                                       ggcgCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTgg                              <  2:540712/150‑1 (MQ=255)
                                                                                                                       ggcgCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTgg                              <  1:2406844/150‑1 (MQ=255)
                                                                                                                        gcgcgcAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGc                             <  1:1899037/150‑1 (MQ=255)
                                                                                                                             cAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCg                        <  1:14194/150‑1 (MQ=255)
                                                                                                                              aaaaGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGa                       <  1:1878845/150‑1 (MQ=255)
                                                                                                                              aaaaGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGa                       <  1:2205439/150‑1 (MQ=255)
                                                                                                                               aaaGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAg                      <  2:1060786/150‑1 (MQ=255)
                                                                                                                                aaGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGTCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAgg                     <  1:803273/150‑1 (MQ=255)
                                                                                                                                aaGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGacg                     <  2:1765466/150‑3 (MQ=255)
                                                                                                                                    gcggcgTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAg                      >  2:1313076/1‑145 (MQ=255)
                                                                                                                                    gcggcgTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAg                      <  1:1313076/145‑1 (MQ=255)
                                                                                                                                       gcgTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCa              <  1:1465767/150‑1 (MQ=255)
                                                                                                                                        atcgctgAGCATGACCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAg             >  2:1381506/7‑150 (MQ=255)
                                                                                                                                           gctgAGCATGACCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCAc          >  2:249958/4‑150 (MQ=255)
                                                                                                                                           aTCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAg                      <  1:2156808/138‑1 (MQ=255)
                                                                                                                                            tCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGATGTTCTCCAGCAcc         >  2:54021/1‑150 (MQ=255)
                                                                                                                                             tgAGCATGACCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAgg                     >  1:1614033/2‑137 (MQ=255)
                                                                                                                                             tgAGCATGACCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAgg                     <  2:1614033/136‑1 (MQ=255)
                                                                                                                                             tgAGCATGACCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAg                      <  2:147232/135‑1 (MQ=255)
                                                                                                                                             tgAGCATGACCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGAGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAg                      >  1:147232/2‑136 (MQ=255)
                                                                                                                                             tgAGCATGACCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCACTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCt        >  1:371187/2‑150 (MQ=255)
                                                                                                                                              gAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAg                                           >  2:328010/1‑114 (MQ=255)
                                                                                                                                              gAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAg                                           <  1:328010/114‑1 (MQ=255)
                                                                                                                                               aGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCTTg      <  2:1985532/150‑1 (MQ=255)
                                                                                                                                                 cATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCTTGGt    <  1:38973/150‑1 (MQ=255)
                                                                                                                                                  aTGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAgg                     >  1:83018/1‑132 (MQ=255)
                                                                                                                                                  aTGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAgg                     <  2:83018/132‑1 (MQ=255)
                                                                                                                                                   tGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGTAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCTTGGTGa  <  2:873613/150‑1 (MQ=255)
                                                                                                                                                   tGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGTTATGCTTGGCGTTCGATGTTCGCCAGCACCTTGGTGa  <  2:1480972/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCTTGGCGCAGCAGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGCTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCTTGGTGA  >  NC_002947/5208581‑5208877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑