Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,237,589 T→A 5.0% M1M (TTG→ATG) †‡ PP_4614 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,237,5890TA 5.0% 388.8 / 8.4 120M1M (TTG→ATG) †‡PP_4614hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (56/57);  new base A (4/2);  total (60/60)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01

Only 100 of 123 total aligned reads displayed.

CGCCAGTTCGCTGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATACCGGGTG  >  NC_002947/5237442‑5237738
                                                                                                                                                      |                                                                                                                                                     
cGCCAGTTCGCTGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCGTTTAAACGGATTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTg                                                                                                                                                     >  1:1741402/1‑150 (MQ=255)
    aGTTCGCTGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCCGGCGCCTTATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAgcc                                                                                                                                                 >  2:2082953‑M2/1‑147 (MQ=255)
        cGCTGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCACAAACGAAAACGCCCGGC‑CTTGAgccgg                                                                                                                                               >  2:2171067‑M2/1‑145 (MQ=255)
         gCTGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAgccgggcg                                                                                                                                            <  1:1862621‑M2/150‑9 (MQ=255)
          cTGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTAACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAgccgggcgt                                                                                                                                           >  1:1641021‑M2/1‑141 (MQ=255)
              gagtggCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTc                                                                                                                                       <  2:2364393/146‑1 (MQ=255)
                  ggCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGttt                                                                                                                                   >  2:2048841/1‑150 (MQ=255)
                  ggCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGttt                                                                                                                                   <  2:1428478/150‑1 (MQ=255)
                   gCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCTTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTtttgtttg                                                                                                                                  >  1:1728392/1‑150 (MQ=255)
                   gCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTtg                                                                                                                                  >  2:852594/1‑150 (MQ=255)
                    cAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGCTCAAGccgggcgttttgcattgc                                                                                                                                   >  1:2252847‑M2/1‑132 (MQ=255)
                      gTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGc                                                                                                                               <  1:1410323/150‑1 (MQ=255)
                          aCGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGAt                                                                                                                           <  1:1670661/150‑1 (MQ=255)
                              tGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAAt                                                                                                                       <  1:252510/150‑1 (MQ=255)
                               gccgccACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATg                                                                                                                      >  1:2215904/1‑150 (MQ=255)
                                ccgccACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATgg                                                                                                                     <  2:1862621/150‑1 (MQ=255)
                                  gccACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGgcg                                                                                                                   <  1:1485690/150‑1 (MQ=255)
                                     accaAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCACAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTg                                                                                                                >  1:1861386/1‑150 (MQ=255)
                                      ccaAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGa                                                                                                               >  1:1799139/1‑150 (MQ=255)
                                          gCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCa                                                                                                           >  1:631650/1‑150 (MQ=255)
                                           cTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGa                                                                                                                                                    >  1:1687559/1‑108 (MQ=255)
                                           cTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGa                                                                                                                                                    <  2:1687559/108‑1 (MQ=255)
                                            tCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAAc                                                                                                         <  1:1694698/150‑1 (MQ=255)
                                            tCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAAc                                                                                                         <  2:724042/150‑1 (MQ=255)
                                              gaTCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACAt                                                                                                       >  2:1655448/2‑150 (MQ=255)
                                              aaTCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTtgt                                                                                                                                 >  1:507661/1‑124 (MQ=255)
                                              aaTCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTtgt                                                                                                                                 <  2:507661/124‑1 (MQ=255)
                                              aaTCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGc                                                                                                                               >  2:1312248/1‑126 (MQ=255)
                                              aaTCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGc                                                                                                                               <  1:1312248/126‑1 (MQ=255)
                                              aaTCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCg                                                                                                                             <  1:1127507/128‑1 (MQ=255)
                                              aaTCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACAt                                                                                                       <  2:1142624/150‑1 (MQ=255)
                                                tCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGc                                                                                                     >  1:1374974/1‑150 (MQ=255)
                                                    gcgcTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAAc                                                                                                 >  2:2149135/1‑150 (MQ=255)
                                                    gcgcTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAAc                                                                                                 <  2:473529/150‑1 (MQ=255)
                                                        tGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGaa                                                                                                              >  2:2039698/1‑133 (MQ=255)
                                                          gAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCgg                                                                                           <  1:2149135/150‑1 (MQ=255)
                                                             cACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCa                                                                                        >  1:1314578/1‑150 (MQ=255)
                                                              aCTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGCGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCaa                                                                                       >  1:2366471/1‑150 (MQ=255)
                                                                ttCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACAt                                                                                                       >  2:1849584/1‑132 (MQ=255)
                                                                ttCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACAt                                                                                                       <  1:1849584/132‑1 (MQ=255)
                                                                 tCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACct                                                                                    >  1:779174/1‑150 (MQ=255)
                                                                      ggCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGGCTGTCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCa                                                                               >  1:761512/1‑150 (MQ=255)
                                                                      ggCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCa                                                                               <  2:1374974/150‑1 (MQ=255)
                                                                            cTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCAtcactc                                                                         >  1:242504/1‑150 (MQ=255)
                                                                             tGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGCTCAAGccgggcgttttgcattgccga                                                                                                                                <  2:224797‑M2/96‑22 (MQ=255)
                                                                             tGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGCTCAAGccgggcgttttgcattgccga                                                                                                                                >  1:224797‑M2/1‑75 (MQ=255)
                                                                             ggcgcAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATc                                                                                                                          <  2:1216743/99‑1 (MQ=255)
                                                                             ggcgcAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATc                                                                                                                          >  1:1216743/2‑100 (MQ=255)
                                                                                            gcgcCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGAc                                                         <  2:2215904/150‑1 (MQ=255)
                                                                                               ccTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCAt                                                      <  1:1655448/150‑1 (MQ=255)
                                                                                                cTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGc                                                                                                                               >  1:1950585/1‑76 (MQ=255)
                                                                                                 ttGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGCTCAAGccgggcgttttgcattgc                                                                                                                                   >  2:1478015‑M2/1‑55 (MQ=255)
                                                                                                 ttGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑Ctt                                                                                                                                                      <  1:945777/52‑1 (MQ=255)
                                                                                                 ttGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑Ctt                                                                                                                                                      >  2:945777/1‑52 (MQ=255)
                                                                                                 ttGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATg                                                                                                      <  1:500832/100‑1 (MQ=255)
                                                                                                 ttGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATg                                                                                                      >  2:500832/1‑100 (MQ=255)
                                                                                                   ggAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGaac                                                  >  1:1095666/1‑150 (MQ=255)
                                                                                                      aCTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGaacaac                                               >  2:1413822/1‑150 (MQ=255)
                                                                                                         gAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCa                                                                   >  1:1470430/1‑127 (MQ=255)
                                                                                                           gTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCAtt                                          <  1:1413822/150‑1 (MQ=255)
                                                                                                            tGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTc                                         >  1:2066970/1‑150 (MQ=255)
                                                                                                            tGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGAGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTc                                         <  2:2370052/150‑1 (MQ=255)
                                                                                                            tGACACGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTc                                         <  1:1613519/150‑1 (MQ=255)
                                                                                                              aCCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTcga                                       >  2:2255732/1‑150 (MQ=255)
                                                                                                               cccGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTcgaa                                      <  1:107627/150‑1 (MQ=255)
                                                                                                               cccGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTcgaa                                      <  2:1095666/150‑1 (MQ=255)
                                                                                                                ccGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGTATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTcgaac                                     >  2:1754372/1‑150 (MQ=255)
                                                                                                                   gACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTcgaacgaa                                  <  2:1861386/150‑1 (MQ=255)
                                                                                                                      gCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCa                               <  2:2251502/150‑1 (MQ=255)
                                                                                                                        cTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCAt                                           <  1:606946/136‑1 (MQ=255)
                                                                                                                        cTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCAt                                           >  2:606946/1‑136 (MQ=255)
                                                                                                                        cTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGt                             <  2:1405131/150‑1 (MQ=255)
                                                                                                                         tGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGtg                            >  1:2365516/1‑150 (MQ=255)
                                                                                                                         tGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGtg                            >  2:1570729/1‑150 (MQ=255)
                                                                                                                         tGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGtg                            >  2:1635385/1‑150 (MQ=255)
                                                                                                                          gATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGtgc                           <  1:852594/150‑1 (MQ=255)
                                                                                                                            tCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAgccgggcgttttcgtttgtgccgatcaatggcgtccgggtcactcagttccaaggcgccgcgcttgctgcgcagcttgccgtagaagtgctccagcgcgtgattgagcttggtggcggcaccg                         <  1:663515‑M2/150‑124 (MQ=255)
                                                                                                                              ggCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGa                                                    >  2:1165203/1‑121 (MQ=255)
                                                                                                                              ggCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGa                                                    <  1:1165203/121‑1 (MQ=255)
                                                                                                                               gCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCAt                      >  1:1455135/1‑150 (MQ=255)
                                                                                                                                cA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCAtt                     >  2:1272974/1‑150 (MQ=255)
                                                                                                                                 a‑aTGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCCATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTc                    <  2:1741402/150‑1 (MQ=255)
                                                                                                                                    tGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCCAGACCATGAACAACCCATTCGAACTAATCAGGGCTGCATTCGc                  <  2:1837101/150‑1 (MQ=255)
                                                                                                                                    tGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTcgaa                                      <  2:839394/130‑1 (MQ=255)
                                                                                                                                     gC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGcc                 <  1:2048841/150‑1 (MQ=255)
                                                                                                                                         aaaCGCCCGGC‑CTTGAgccgggcgttttgcattgccgatcaggcgtccgggtcactcagttccaaggcgccgcgcttgctgcgcagcttgccgtagaagtgctccagcgcgtgattga                                              <  1:458312‑M2/118‑103 (MQ=255)
                                                                                                                                         aaaCGCCCGGC‑CTTGAgccgggcgttttgcattgccgatcaggcgtccgggtcactcagttccaaggcgccgcgcttgctgcgcagcttgccgta                                                                     >  2:1034374‑M2/1‑16 (MQ=255)
                                                                                                                                         aaaCGCCCGGC‑CTTGAgccgggcgttttgcattgccgatcaggcgtccgggtcactcagttccaaggcgccgcgcttgctgcgcagcttgccgta                                                                     <  1:1034374‑M2/95‑80 (MQ=255)
                                                                                                                                         aaaCGCCCGGC‑CTTGAgccgggcgttttgcattgccgatcaggcgtccgggtcactcagttccaaggcgccgcgcttgctgcgcagcttgcc                                                                        <  1:177539‑M2/92‑77 (MQ=255)
                                                                                                                                         aaaCGCCCGGC‑CTTGAgccgggcgttttgcattgccgatcaggcgtccgggtcactcagttccaaggcgccgcgcttgctgcgcagcttgcc                                                                        >  2:177539‑M2/1‑16 (MQ=255)
                                                                                                                                          aaCGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGc                                                                                >  1:684341/1‑83 (MQ=255)
                                                                                                                                             gCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAAt          >  1:2401276/1‑150 (MQ=255)
                                                                                                                                               ccGGC‑CTTGAgccgggcgttttgcattgccgatcaggcgtccgggtcactcagttccaaggcgccgcgcttgctgcgcagcttgccgtagaagtgctccagcgcgtgattgagcttggtggcggcaccgtcgactgcctggtccagcgaa        <  1:2013079‑M2/150‑141 (MQ=255)
                                                                                                                                                cGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATAcc       <  2:242504/150‑1 (MQ=255)
                                                                                                                                                cGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATAcc       >  1:1754321/1‑150 (MQ=255)
                                                                                                                                                cGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATAcc       >  2:1924780/1‑150 (MQ=255)
                                                                                                                                                cGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATAcc       >  1:1304974/1‑150 (MQ=255)
                                                                                                                                                  gC‑CTTGAgccgggcgttttcgtttgtgccgatcaggcgtccgggtcactcagttccaaggcgccgcgctt                                                                                     <  1:23360‑M2/70‑64 (MQ=255)
                                                                                                                                                  gC‑CTTGAgccgggcgttttcgtttgtgccgatcaggcgtccgggtcactcagttccaaggcgccgcgctt                                                                                     >  2:23360‑M2/1‑7 (MQ=255)
                                                                                                                                                      ttGAGCCGGGCGTTTTCGTTTGTGCAGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATACCGGGTg  >  1:242056/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGCCAGTTCGCTGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCA‑ATGC‑AAAACGCCCGGC‑CTTGAGCCGGGCGTTTTCGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATACCGGGTG  >  NC_002947/5237442‑5237738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑