BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	444,433	C→A	5.3%	P198Q (CCG→CAG)	bioD →	dethiobiotin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	444,433	0	C	A	5.3%	343.3 / 2.9	104	P198Q (CCG→CAG)	bioD	dethiobiotin synthetase
Reads supporting (aligned to +/- strand): ref base C (49/49); new base A (4/2); total (53/51)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CGGTTGGGTTGCATCAGCCATGCCTTGCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGAGGGAACGCATAC  >  NC_002947/444288‑444578

cGGTTGGGTTGCATCAGCCATGCCTTGCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGgcg                                                                                                                                               <  2:1334359/150‑1 (MQ=255)

  gttgggttgCATCAGCCATGCCTTGCTCAGCGCCGAACAAATCGAGCGTGATGGTCTGCAGTTGGCGGGTTCGGGACCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGcc                                                                                                                                             <  1:540544/150‑1 (MQ=255)

       gttgCATCAGCCATGCCTTGCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGcc                                                                                                                                        <  1:755470/150‑1 (MQ=255)

         tgCATCAGCCATGCCTTGCTCAGCGCCGAAGCGATCGGGCGTGATGGGCTGCTGTTGGTGGGTTGGGTAGCAAACATCATCGAGTCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTg                                                                                                                                      <  2:1045360/150‑1 (MQ=255)

            agcAGCCATGCCTTGCTCAGGGCCGAGGCGATCGAGCGGGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTgggg                                                                                                                                   >  2:32147/3‑150 (MQ=255)

            aTCAGCCATGCCTTGCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTgggg                                                                                                                                   >  2:2030806/1‑150 (MQ=255)

               acccATGCCTTGCTCAGTGCCGAAGCGATCGAGTTTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATTATCGAGCCGCGCATCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCgg                                                                                                                                <  2:737916/148‑1 (MQ=255)

                  cATGCCTTGCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGtt                                                                                                                             <  1:2030806/150‑1 (MQ=255)

                       cTTGCTCAGCGCCGAAGCGATCGAGCGTGGTGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCaa                                                                                                                        >  1:1643147/1‑150 (MQ=255)

                       cTTGCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCaa                                                                                                                        <  2:1227112/150‑1 (MQ=255)

                       cTTGCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCaa                                                                                                                        <  1:2043404/150‑1 (MQ=255)

                        ttGCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGACCCTGGCAAGCCTTGCTGCTCGCACTCCGTCTCCTTGCCTGGGGCGGGTTCCCAAg                                                                                                                       >  2:1833013/1‑150 (MQ=255)

                        ttGCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAg                                                                                                                       >  1:2114455/1‑150 (MQ=255)

                         tGCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGc                                                                                                                      <  2:318654/150‑1 (MQ=255)

                          gCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCt                                                                                                                     >  1:1609085/1‑150 (MQ=255)

                            tCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCa                                                                                                                   >  2:614851/1‑150 (MQ=255)

                            tCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCa                                                                                                                   >  2:1154112/1‑150 (MQ=255)

                            tCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGATAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCgg                                                                                                                                >  2:1635174/1‑137 (MQ=255)

                            tCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGATAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCgg                                                                                                                                <  1:1635174/137‑1 (MQ=255)

                                    gAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGcc                                                                                                           >  2:1405822/1‑150 (MQ=255)

                                     aaGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCa                                                                                                          <  2:59108/150‑1 (MQ=255)

                                     aaGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCa                                                                                                          >  1:59108/1‑150 (MQ=255)

                                      aGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAg                                                                                                                       >  2:2215349/1‑136 (MQ=255)

                                      aGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAg                                                                                                                       <  1:2215349/136‑1 (MQ=255)

                                             gAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGAc                                                                                                  >  1:1792155/1‑150 (MQ=255)

                                             gAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGAc                                                                                                  >  2:1530268/1‑150 (MQ=255)

                                             gAGCGGGATGGGCGGCATTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGAc                                                                                                  >  2:1241827/1‑150 (MQ=255)

                                                 gTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCggg                                                                                                                               >  2:2076580/1‑117 (MQ=255)

                                                 gTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCggg                                                                                                                               <  1:2076580/117‑1 (MQ=255)

                                                  tGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACAtggt                                                                                             <  2:758202/150‑1 (MQ=255)

                                                   gATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCACGCGCGTCGGCTGGGGGGGGGTCCCAAGCTCAAGCAGGCCAGTGCCGACAtggtg                                                                                            >  1:541936/1‑150 (MQ=255)

                                                        gCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCGGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGa                                                                                       >  2:1614082/1‑150 (MQ=255)

                                                         cTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTgg                                                                                                                                     <  1:408131/103‑1 (MQ=255)

                                                         cTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTgg                                                                                                                                     >  2:408131/1‑103 (MQ=255)

                                                           gCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCa                                                                                    >  1:2029170/1‑150 (MQ=255)

                                                              gTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGATGAGCATCt                                                                                 >  2:885994/1‑150 (MQ=255)

                                                              gTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCGTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCATTGCAGTGCTGACATGGTGGTTGAGCATCt                                                                                 <  2:25953/150‑1 (MQ=255)

                                                               ttGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCgg                                                                                                                                <  1:1010079/102‑1 (MQ=255)

                                                                 ggCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCa                                                                              >  2:1245609/1‑150 (MQ=255)

                                                                  gCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGTGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCaa                                                                             <  2:1557230/150‑1 (MQ=255)

                                                                  gCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCaa                                                                             >  1:386643/1‑150 (MQ=255)

                                                                  gCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCaa                                                                             <  1:32454/150‑1 (MQ=255)

                                                                        tGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGAt                                                                       <  2:1643147/150‑1 (MQ=255)

                                                                          ggTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATct                                                                     <  1:1154112/150‑1 (MQ=255)

                                                                            tAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATctgc                                                                   <  2:386643/150‑1 (MQ=255)

                                                                                aaaCATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCa                                                                                                                   <  1:1169832/98‑1 (MQ=255)

                                                                                aaaCATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCa                                                                                                                   >  2:1169832/1‑98 (MQ=255)

                                                                                aaaCATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGa                                                               >  2:2370362/1‑150 (MQ=255)

                                                                                aaaCATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGa                                                               >  1:528559/1‑150 (MQ=255)

                                                                                 aaCATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGAt                                                              <  1:885994/150‑1 (MQ=255)

                                                                                   catcatCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTa                                                            >  2:287956/1‑150 (MQ=255)

                                                                                    atcatcGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAg                                                           >  1:643575/1‑150 (MQ=255)

                                                                                     tcatcGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGTGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGt                                                          <  2:892387/150‑1 (MQ=255)

                                                                                     tcatcGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGt                                                          <  2:528559/150‑1 (MQ=255)

                                                                                        tcGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGa                                                                                       >  1:928219/1‑118 (MQ=255)

                                                                                        tcGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGa                                                                                       <  2:928219/118‑1 (MQ=255)

                                                                                        tcGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTg                                                       >  2:1238791/1‑150 (MQ=255)

                                                                                          gAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCCGCCATGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGtt                                                     <  1:1833013/150‑1 (MQ=255)

                                                                                          gAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGtt                                                     <  1:602976/150‑1 (MQ=255)

                                                                                             ccGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCa                                                  <  1:2370362/150‑1 (MQ=255)

                                                                                              cgcgCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAg                                                 <  2:2029170/150‑1 (MQ=255)

                                                                                               gcgcACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAgg                                                >  1:2320793/1‑150 (MQ=255)

                                                                                                     cTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAAc                                                                            <  2:977523/116‑1 (MQ=255)

                                                                                                     cTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAAc                                                                            >  1:977523/1‑116 (MQ=255)

                                                                                                      tCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGcc                                         >  1:2103912/1‑150 (MQ=255)

                                                                                                             tGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTcgc                                  <  1:1241827/150‑1 (MQ=255)

                                                                                                               gAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCgg                                <  1:1614082/150‑1 (MQ=255)

                                                                                                               gAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCgg                                >  2:233267/1‑150 (MQ=255)

                                                                                                               gAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCgg                                <  1:479662/150‑1 (MQ=255)

                                                                                                               gAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCgg                                <  2:2008788/150‑1 (MQ=255)

                                                                                                                 agagAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTgg                                                                         >  1:171337/1‑107 (MQ=255)

                                                                                                                 agagAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGt                              >  1:2134394/1‑150 (MQ=255)

                                                                                                                 agagAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGt                              >  2:1664593/1‑150 (MQ=255)

                                                                                                                  gagaACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCg                                            >  1:826512/1‑135 (MQ=255)

                                                                                                                  gagaACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCg                                            <  2:826512/135‑1 (MQ=255)

                                                                                                                  gagaACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTa                             <  2:1805638/150‑1 (MQ=255)

                                                                                                                     aaCCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAAc                          >  1:970570/1‑150 (MQ=255)

                                                                                                                             cAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCt                  >  1:2122825/1‑150 (MQ=255)

                                                                                                                                ccTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCCGGTAAACCCGCTCCTACg               >  2:954672/1‑150 (MQ=255)

                                                                                                                                 cTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGa              <  2:1609085/150‑1 (MQ=255)

                                                                                                                                  ttGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTAc                <  2:543431/147‑1 (MQ=255)

                                                                                                                                  ttGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTAc                >  1:543431/1‑147 (MQ=255)

                                                                                                                                  ttGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCATATTCGCGGGTAAACCCGCTCCTACGAg             <  1:1530268/150‑1 (MQ=255)

                                                                                                                                    gCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGa                                                                                       <  1:1326546/74‑1 (MQ=255)

                                                                                                                                    gCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGa                                                                                       >  2:1326546/1‑74 (MQ=255)

                                                                                                                                      agagCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGAGGGaa         >  1:458078/1‑150 (MQ=255)

                                                                                                                                      aaggcgcCGGCAGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGc                                          >  1:2031945/4‑117 (MQ=255)

                                                                                                                                      aaggcgcCGGCAGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGc                                          <  2:2031945/114‑1 (MQ=255)

                                                                                                                                      aaggcgcCGGCAGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCgg                                <  2:6749/124‑1 (MQ=255)

                                                                                                                                      aaggcgcCGGCAGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCgg                                >  1:6749/4‑127 (MQ=255)

                                                                                                                                      aaggcgcCGGCAGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCAGCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGAGGGaa         >  1:1144392/4‑150 (MQ=255)

                                                                                                                                       gagCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTCAGCCCGCTCCGAGGAGGGAAc        >  1:410629/1‑150 (MQ=255)

                                                                                                                                       gagCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGAGGGAAc        <  2:2134394/150‑1 (MQ=255)

                                                                                                                                       gagCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGAGGGAAc        <  1:1405822/150‑1 (MQ=255)

                                                                                                                                         gcgcCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGAGGGAACGc      <  1:1664593/150‑1 (MQ=255)

                                                                                                                                         gcgcCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGAGGGAACGc      <  2:970570/150‑1 (MQ=255)

                                                                                                                                          cgcCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGAGGGAACGCa     <  2:2114455/150‑1 (MQ=255)

                                                                                                                                             cTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGAGGGAACGCATAc  >  1:1286377/1‑150 (MQ=255)

                                                                                                                                                 cGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCAt                                                                                   <  1:2055551/65‑1 (MQ=255)

                                                                                                                                                 cGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCAt                                                                                   >  2:2055551/1‑65 (MQ=255)

CGGTTGGGTTGCATCAGCCATGCCTTGCTCAGCGCCGAAGCGATCGAGCGTGATGGGCTGCAGTTGGCGGGTTGGGTAGCAAACATCATCGAGCCGCGCACCTCACGCCTGGAAGAGAACCTGGCCAGCCTTGCAGAGCGCCTGCCGGCGCCTTGCCTGGGGCGGGTTCCCAAGCTCAAGCAGGCCAGTGCCGACATGGTGGCTGAGCATCTGCAACTGGATCTGCTGGATTAGTCTGTTGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGAGGGAACGCATAC  >  NC_002947/444288‑444578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`