Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,261,125 A→C 7.4% L175R (CTG→CGG)  PP_4637 ← 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,261,1250AC 7.4% 326.8 / 15.2 119L175R (CTG→CGG) PP_46375‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (54/55);  new base C (2/7);  total (57/62)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

Only 100 of 119 total aligned reads displayed.

CCAGGGTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGTTCATC  >  NC_002947/5260979‑5261273
                                                                                                                                                  |                                                                                                                                                    
ccAGGGTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGt                                                                                                                                                   >  2:2045355/1‑150 (MQ=255)
ccAGGGTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGt                                                                                                                                                   <  1:775196/150‑1 (MQ=255)
ccAGGGTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGt                                                                                                                                                   >  1:561095/1‑150 (MQ=255)
 cAGGGTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGtt                                                                                                                                                  >  2:1205539/1‑150 (MQ=255)
  aGGGTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTg                                                                                                                                                 <  1:2316282/150‑1 (MQ=255)
    ggTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCACCCGGCAGGTTtgc                                                                                                                                               <  2:33001/150‑1 (MQ=255)
    ggTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCTGCCGGTTgat                                                                                                                                               <  2:2110824/150‑3 (MQ=255)
       cTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCTGCCGGTTgatggc                                                                                                                                            <  2:2365080/150‑6 (MQ=255)
       cTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCTGCCGGTTgatggc                                                                                                                                            <  1:1222328/150‑6 (MQ=255)
        ttGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGcc                                                                                                                                           >  1:2227394/1‑150 (MQ=255)
        ttGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGcc                                                                                                                                           >  1:1916418/1‑150 (MQ=255)
        ttGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGcc                                                                                                                                           >  1:1453972/1‑150 (MQ=255)
         tGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCa                                                                                                                                          <  1:2247570/150‑1 (MQ=255)
            ccAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGc                                                                                                                                       <  2:561095/150‑1 (MQ=255)
            ccAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGc                                                                                                                                       >  1:1712766/1‑150 (MQ=255)
            ccAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCGGAACCGGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGc                                                                                                                                       >  1:1468481/1‑150 (MQ=255)
             cAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTACTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAg                                                                                                                                                     <  1:1908013/135‑1 (MQ=255)
             cAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTACTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAg                                                                                                                                                     >  2:1908013/1‑135 (MQ=255)
               aaTCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGa                                                                                                                                    <  2:1010557/150‑1 (MQ=255)
                 gcacaAGGCGCGGCGTGGCGACCGGCGACCAGGCGCAGCGACAGTACTTTGTAGGGCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGGGTCGGAACCCGATCGAGGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAgg                                                                                                                                  <  2:696159/149‑1 (MQ=255)
                   acaAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCTACTTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGtt                                                                                                                                >  2:1475592/1‑150 (MQ=255)
                   acaAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCGCCAACCTGCCGGTTgatggc                                                                                                                                            <  2:2358867/138‑6 (MQ=255)
                   acaAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCGCCAACCTGCCGGTTgatggc                                                                                                                                            >  1:2358867/1‑133 (MQ=255)
                   acaAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGtt                                                                                                                                <  2:290739/150‑1 (MQ=255)
                   acaAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGtt                                                                                                                                >  1:167873/1‑150 (MQ=255)
                   acaAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGtt                                                                                                                                >  1:1752070/1‑150 (MQ=255)
                   acaAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGtt                                                                                                                                >  1:2050547/1‑150 (MQ=255)
                    caAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTg                                                                                                                               >  1:1342990/1‑150 (MQ=255)
                           aggcgTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCa                                                                                                                        <  2:2171009/150‑1 (MQ=255)
                            ggcgTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGTCGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAg                                                                                                                       <  1:1985885/150‑1 (MQ=255)
                            ggcgTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAg                                                                                                                       <  1:535149/150‑1 (MQ=255)
                            ggcgTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAg                                                                                                                       <  2:1041953/150‑1 (MQ=255)
                               gTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGcc                                                                                                                    >  2:1046665/1‑150 (MQ=255)
                                 tGCGACCGTCGACCAGGCCTAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTTGCGGCCAGGCCTATGTTGCCTTCCAGGCCAc                                                                                                                  <  2:500533/150‑1 (MQ=255)
                                     aCCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCCGGTTGGCGGCCAGGCAGAGGTTGCCTTCCAGGCCCCCGaa                                                                                                              <  2:343239/150‑1 (MQ=255)
                                       cGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGt                                                                                                            <  2:1712766/150‑1 (MQ=255)
                                         tCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAgaag                                                                                                          >  1:610726/1‑147 (MQ=255)
                                             ccAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTgg                                                                                                      <  1:871629/150‑1 (MQ=255)
                                                ggCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGaa                                                                                                              >  2:515511/1‑139 (MQ=255)
                                                ggCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGaa                                                                                                              <  1:515511/139‑1 (MQ=255)
                                                   ccAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCa                                                                                                >  2:321444/1‑150 (MQ=255)
                                                   ccAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCa                                                                                                >  1:1095988/1‑150 (MQ=255)
                                                   ccAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCa                                                                                                <  1:1746001/150‑1 (MQ=255)
                                                        gACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCTGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGcttc                                                                                           <  2:90366/150‑1 (MQ=255)
                                                        gACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGAGTAGcttc                                                                                           >  1:1307852/1‑150 (MQ=255)
                                                         aCAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGcttct                                                                                          >  1:1103328/1‑150 (MQ=255)
                                                          cAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGcttctt                                                                                         <  2:167873/150‑1 (MQ=255)
                                                            gTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCCCCGAAGTACGTGGCGATCAGCTTCTTCa                                                                                       >  2:925714/1‑150 (MQ=255)
                                                             tACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGAGCCTGACCCAGATCGACGTGCAGGCCAGCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCaa                                                                                      >  1:2100821/1‑150 (MQ=255)
                                                               ccTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACg                                                                                    <  2:2227394/150‑1 (MQ=255)
                                                                cTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCTGCCGGTTgatg                                                                                                                                              >  2:1611536/1‑88 (MQ=255)
                                                                cTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCTGCCGGTTgatg                                                                                                                                              <  1:1611536/91‑4 (MQ=255)
                                                                 ttGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGt                                                                                  >  1:1412805/1‑150 (MQ=255)
                                                                   gTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGAGCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCATGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTgc                                                                                <  2:112759/150‑1 (MQ=255)
                                                                         cGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTg                                                                          >  1:1043209/1‑150 (MQ=255)
                                                                              ggCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGtt                                                                           >  1:2173237/1‑144 (MQ=255)
                                                                               gCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGAt                                                                    <  1:1581005/150‑1 (MQ=255)
                                                                                 gATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGAtgt                                                                  <  1:1071722/150‑1 (MQ=255)
                                                                                   tCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGcttct                                                                                          >  2:2147805/1‑124 (MQ=255)
                                                                                   tCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGcttct                                                                                          <  1:2147805/124‑1 (MQ=255)
                                                                                   tCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGAtgttg                                                                <  2:665671/150‑1 (MQ=255)
                                                                                   tCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGCCCAgg                                                                                                                                        <  2:1227606/78‑1 (MQ=255)
                                                                                   tCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGCCCAgg                                                                                                                                        >  1:1227606/1‑78 (MQ=255)
                                                                                    cGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGAtgttgt                                                               <  1:1156443/150‑1 (MQ=255)
                                                                                      agaATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAGTTACGTTGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAg                                                             >  2:1871941/1‑150 (MQ=255)
                                                                                       gaATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGaa                                                                        <  1:1107636/138‑1 (MQ=255)
                                                                                       gaATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGaa                                                                        >  2:1107636/1‑138 (MQ=255)
                                                                                            gTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGt                                                       >  1:1421264/1‑150 (MQ=255)
                                                                                               tGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCa                                                    >  2:57554/1‑150 (MQ=255)
                                                                                               tGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTAGAGCTCCGATTATTCAAAGGCGCGCGTGGAAGGAGGTTGTAGACGCGTTCa                                                    >  2:2070067/1‑150 (MQ=255)
                                                                                                ggTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCAt                                                   <  2:1752070/150‑1 (MQ=255)
                                                                                                 gTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATa                                                  <  2:1421264/150‑1 (MQ=255)
                                                                                                  tACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAg                                                 <  2:1060437/150‑1 (MQ=255)
                                                                                                     tGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGtt                                                                                                                                >  1:874131/1‑68 (MQ=255)
                                                                                                     tGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGtt                                                                                                                                <  2:874131/68‑1 (MQ=255)
                                                                                                     tGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCg                                              >  2:2240250/1‑150 (MQ=255)
                                                                                                      gCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGt                                             >  2:991990/1‑150 (MQ=255)
                                                                                                              gcCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAg                                     >  2:1053037/1‑150 (MQ=255)
                                                                                                                  gAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCt                                 >  1:1323098/1‑150 (MQ=255)
                                                                                                                    aCCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTgg                               <  2:2216793/150‑1 (MQ=255)
                                                                                                                     ccAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGTCCGCGGTTGCCTTCCAGGACACCGAAGTACGTGGCGATAAGCTACTTCAATGGTACGCGTTGAAGGATGTTGTAGACGCGTTCATCGGCGTTTTTCCAGTcccgag                              >  2:381147/1‑146 (MQ=255)
                                                                                                                     ccAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCCGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATTGGCGTTTTTCCAGTCCTggg                              >  1:896421/1‑150 (MQ=255)
                                                                                                                       aGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGc                            <  2:1916418/150‑1 (MQ=255)
                                                                                                                          tCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAgc                         <  1:49143/150‑1 (MQ=255)
                                                                                                                               gTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGaa                                                                                                              <  2:1569315/60‑1 (MQ=255)
                                                                                                                                 gCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTggg                  <  1:2070067/150‑1 (MQ=255)
                                                                                                                                  cAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTCGAAGGATGTTGTAGCCGCGTTCATAGGCGTTTTTCCAGTCCTGGGCCAGCGCCTgcgc                 >  2:2259166/1‑150 (MQ=255)
                                                                                                                                   aGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCg                >  1:1563200/1‑150 (MQ=255)
                                                                                                                                    ggCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGa               <  2:1043209/150‑1 (MQ=255)
                                                                                                                                        aTCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGAt           <  2:2100821/150‑1 (MQ=255)
                                                                                                                                         tCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATg          >  1:2159689/1‑150 (MQ=255)
                                                                                                                                           aaCCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATggg        >  2:1522926/1‑150 (MQ=255)
                                                                                                                                             ccGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGtt      <  1:2240250/150‑1 (MQ=255)
                                                                                                                                              cGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGGAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGTTc     >  2:1747330/1‑150 (MQ=255)
                                                                                                                                               ggCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGTTCa    >  2:2038448/1‑150 (MQ=255)
                                                                                                                                                gCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGTTCAt   <  2:1453972/150‑1 (MQ=255)
                                                                                                                                                 cAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGGGATCAGCTTCTTCAACGGTGCGCGTTGaa                                                                        >  1:1979236/1‑80 (MQ=255)
                                                                                                                                                 cAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGaa                                                                        <  2:1979236/80‑1 (MQ=255)
                                                                                                                                                 cAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGTTCATc  <  1:67224/150‑1 (MQ=255)
                                                                                                                                                 cAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGTTCATc  <  1:381147/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCAGGGTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGTTCATC  >  NC_002947/5260979‑5261273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑