| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,362,263 | C→G | 8.2% | V49L (GTG→CTG) | tpiA ← | triose phosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,362,263 | 0 | C | G | 8.2% | 266.1 / 12.6 | 85 | V49L (GTG→CTG) | tpiA | triose phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (39/39); new base G (3/4); total (42/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
GCTCCGAATGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCAT > NC_002947/5362116‑5362407 | gCTCCGAATGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCt < 1:947927/150‑1 (MQ=255) tCCGAATGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCtga > 2:1028997/1‑150 (MQ=255) cGAATGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCtgatt < 2:1258567/150‑1 (MQ=255) gAATGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCtgattg > 2:1180975/1‑150 (MQ=255) aTGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCtgattgat < 1:1028997/150‑1 (MQ=255) tGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCAGGTGATtgatg < 2:422902/150‑1 (MQ=255) tGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATtgatg < 2:2165278/150‑1 (MQ=255) gCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCtgattgat > 2:998434/1‑147 (MQ=255) gCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCtgattgat < 1:998434/147‑1 (MQ=255) ccAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCAGGTGATtgatggcc < 1:1130347/150‑4 (MQ=255) ccAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAAc > 2:82132/1‑150 (MQ=255) aaTCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGTCATCAATCAGGTGATtgatggcct < 2:707816/149‑5 (MQ=255) aaTCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGTCATCAATCAGGTGATtgatggcct > 1:707816/1‑145 (MQ=255) aTCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGCTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAg < 1:1000825/150‑1 (MQ=255) acacCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCg < 2:1178148/150‑1 (MQ=255) cAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTgg > 1:1773604/1‑150 (MQ=255) cAACTTGCAACCTGATTCAACCAGCTGCTCGGGAGCAACTTCCCCGTTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTgg > 2:1421483/1‑150 (MQ=255) tGCAACCTCCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGCCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGCGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCACTCACCTGATTGATGAACAAGGCCGGTGGAAATa < 2:132865/150‑1 (MQ=255) cAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTGCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACg < 2:2337555/150‑1 (MQ=255) aaCCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACgg > 2:47309/1‑150 (MQ=255) aCCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGc < 2:1773604/150‑1 (MQ=255) tGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGAc > 1:2397201/1‑150 (MQ=255) gCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACt > 1:746345/1‑150 (MQ=255) cTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAAGCCATCAATCACCTGACTGATAAACAAGGCCGTTGGAAATACGGCGACtt > 2:162177/1‑150 (MQ=255) ttCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTc > 2:1635221/1‑150 (MQ=255) ttCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTc > 2:768310/1‑150 (MQ=255) cAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCa < 1:155177/150‑1 (MQ=255) aaCCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTTTTTGAACGGCCTGG‑GATGAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCAc > 1:2090057/1‑150 (MQ=255) aCCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACt > 2:584461/1‑150 (MQ=255) ccAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACgg > 1:108965/1‑138 (MQ=255) ccAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACgg < 2:108965/138‑1 (MQ=255) cAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTc < 2:479848/149‑1 (MQ=255) cAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTc > 1:479848/1‑149 (MQ=255) aGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCg > 1:316772/1‑150 (MQ=255) gctCGGGAGCAACTTCCCCGTTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATGCGGCGACTTCCACTCCGCTCg < 1:1421483/150‑1 (MQ=255) gggAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTg < 1:650697/119‑1 (MQ=255) gggAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTg > 2:650697/1‑119 (MQ=255) cTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGAt < 2:746345/150‑1 (MQ=255) ttCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCAGGTGATtgatg < 2:2303125/97‑1 (MQ=255) ttCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCAGGTGATtgatg > 1:2303125/1‑97 (MQ=255) ttCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGAtt > 2:114603/1‑150 (MQ=255) ccccGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTAc > 1:1020077/1‑150 (MQ=255) ccGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑aa > 1:306452/1‑137 (MQ=255) ccGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑aa < 2:306452/137‑1 (MQ=255) ccGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTc > 1:115365/1‑150 (MQ=255) ggTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACTAGGCGCGTCTAAAGACGGCTACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCaa < 1:47309/150‑1 (MQ=255) ggTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCaa < 2:1906843/150‑1 (MQ=255) gcACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTg < 1:82132/150‑1 (MQ=255) aCCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGt > 2:2151877/1‑150 (MQ=255) cccTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGtc < 1:1673650/150‑1 (MQ=255) ccTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGtca > 1:1031682/1‑150 (MQ=255) ggTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTc < 1:714159/101‑1 (MQ=255) ggTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTc > 2:714159/1‑101 (MQ=255) aCAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTa < 2:1402029/150‑1 (MQ=255) cagcagAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTgg < 1:1261163/132‑1 (MQ=255) cagcagAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTgg > 2:1261163/1‑132 (MQ=255) agcagAATTCTGTGCGCCTACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACt > 2:1844120/1‑97 (MQ=255) agcagAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACt < 1:1844120/97‑1 (MQ=255) gAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCgg < 2:2090057/150‑1 (MQ=255) gAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCgg < 1:114603/150‑1 (MQ=255) aaTTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTg < 1:2150379/125‑1 (MQ=255) aaTTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTg > 2:2150379/1‑125 (MQ=255) tgtgCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCg > 2:1620396/1‑150 (MQ=255) gcCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGCCAAACGGCAAACCGTTGAAG‑ATCGGGTGAGCTCAGCGACGCTAGCGCGGGTACCGTGCAt > 1:2341405/1‑150 (MQ=255) aCAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGa > 1:281037/1‑124 (MQ=255) aCAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGa < 2:281037/124‑1 (MQ=255) aCAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTc < 2:115365/150‑1 (MQ=255) aGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCa < 2:1020077/150‑1 (MQ=255) aGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCa < 1:1914465/150‑1 (MQ=255) gTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCAttt > 2:1326205/1‑145 (MQ=255) gTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCAttt < 1:1326205/145‑1 (MQ=255) tAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTg < 2:2042336/107‑1 (MQ=255) tAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTg > 1:2042336/1‑107 (MQ=255) ttt‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGCCTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGc > 1:1591306/1‑150 (MQ=255) ttt‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGc > 2:1952789/1‑150 (MQ=255) cTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGAt > 1:1280555/1‑81 (MQ=255) cTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGAt < 2:1280555/81‑1 (MQ=255) cAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAgg < 2:2147802/150‑1 (MQ=255) cAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AACGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAgg < 1:1620396/150‑1 (MQ=255) ggCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGc < 1:768310/150‑1 (MQ=255) ggCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTCACCATCGAGCGTAGGGc > 2:441750/1‑150 (MQ=255) ttcATCAATCAGGTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCg > 1:2385677/3‑150 (MQ=255) aatcaCCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCaa < 2:1444672/75‑1 (MQ=255) aatcaCCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCaa > 1:1444672/1‑75 (MQ=255) aatcaCCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCAt > 1:724088/1‑150 (MQ=255) | GCTCCGAATGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTT‑ACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGC‑AAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCAT > NC_002947/5362116‑5362407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |