Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,450,258 C→T 5.4% Q30* (CAG→TAG)  ybeY → endoribonuclease YbeY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,450,2580CT 5.4% 247.4 / 2.6 86Q30* (CAG→TAG) ybeYendoribonuclease YbeY
Reads supporting (aligned to +/- strand):  ref base C (39/41);  new base T (2/3);  total (41/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

TATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGACCTGCTCGATATCC  >  NC_002947/5450109‑5450402
                                                                                                                                                       |                                                                                                                                                
tATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGGCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGcc                                                                                                                                                  >  1:1162181/1‑150 (MQ=255)
    ttcgAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCcagcg                                                                                                                                              >  1:756563/2‑150 (MQ=255)
    gtcgAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCcagcg                                                                                                                                              >  1:848585/1‑150 (MQ=255)
        aaGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCcagcgcagc                                                                                                                                          <  1:2356016/150‑1 (MQ=255)
        aaGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCcagcgcagc                                                                                                                                          >  1:741866/1‑150 (MQ=255)
        aaGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCcagcgcagc                                                                                                                                          >  2:2066279/1‑150 (MQ=255)
        aaGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCcagcgcagc                                                                                                                                          >  2:2285912/1‑150 (MQ=255)
          gCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCagcgcagcgc                                                                                                                                        <  2:848585/150‑1 (MQ=255)
            cTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGcc                                                                                                                                       <  1:2314312/149‑1 (MQ=255)
            cTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGcc                                                                                                                                       >  2:2314312/1‑149 (MQ=255)
            cTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCg                                                                                                                                      >  2:2097832/1‑150 (MQ=255)
             tACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGATGGTGAGA‑‑AATGGCATTGCGGcaccgcactgcgta                                                                                                                                     >  2:518619/1‑143 (MQ=255)
                gACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTc                                                                                                                                  >  2:2224522/1‑150 (MQ=255)
                  ccGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAAGTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGATGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCgg                                                                                                                                <  1:2300316/150‑1 (MQ=255)
                  ccGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCgg                                                                                                                                >  1:1536615/1‑150 (MQ=255)
                    gCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCg                                                                                                                                 <  1:69681/147‑1 (MQ=255)
                    gCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCg                                                                                                                                 >  2:69681/1‑147 (MQ=255)
                      ttCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGCCTCGGCAAt                                                                                                                            >  1:1298747/1‑150 (MQ=255)
                        cGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGa                                                                                                                          <  2:2341133/150‑1 (MQ=255)
                         gAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGGTATCCACCGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGAc                                                                                                                         <  1:2374915/150‑1 (MQ=255)
                         gAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGAc                                                                                                                         >  1:145806/1‑150 (MQ=255)
                              ccGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTc                                                                                                                    >  1:707224/1‑150 (MQ=255)
                               cGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCg                                                                                                                   >  2:2007645/1‑150 (MQ=255)
                                  cAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAATACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCt                                                                                                                <  2:205695/150‑1 (MQ=255)
                                    gCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTgg                                                                                                              >  2:2006740/1‑150 (MQ=255)
                                     cccAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGCTGCGCTGGCGCAACGccaattc                                                                                                                                  <  1:594527/129‑6 (MQ=255)
                                     cccAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGCTGCGCTGGCGCAACGccaattc                                                                                                                                  >  2:594527/1‑124 (MQ=255)
                                      ccAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTc                                                                                                            <  2:2368802/150‑1 (MQ=255)
                                        aaGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCcagcgcagc                                                                                                                                          <  2:1432977/118‑1 (MQ=255)
                                        aaGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCcagcgcagc                                                                                                                                          >  1:1432977/1‑118 (MQ=255)
                                         aGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGACAACGCCCCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTcgac                                                                                                         <  1:294198/150‑1 (MQ=255)
                                         aGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTcgac                                                                                                         <  2:1536615/150‑1 (MQ=255)
                                            ccGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGaa                                                                                                      <  1:1237323/150‑1 (MQ=255)
                                             cGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAg                                                                                                     <  1:1726294/150‑1 (MQ=255)
                                              gAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACCGCCCACGGATGACGCCCCTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGACGACTCCGAAATGACCATTCGCCTGGTCGACGAAGc                                                                                                    <  1:2344552/150‑1 (MQ=255)
                                                  cGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGaa                                                                                                <  1:2006740/150‑1 (MQ=255)
                                                    tCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCTCCAGCGCAGCGCCGACTCGGaa                                                                                                                              >  1:1835800/1‑118 (MQ=255)
                                                    tCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGaa                                                                                                                              <  2:1835800/118‑1 (MQ=255)
                                                    tCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAgg                                                                                              <  1:2066279/150‑1 (MQ=255)
                                                        gCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCcgc                                                                                          >  2:1301494/1‑150 (MQ=255)
                                                        gCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCcgc                                                                                          >  1:1579864/1‑150 (MQ=255)
                                                          aaaGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGAGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGa                                                                                        >  2:2246061/1‑150 (MQ=255)
                                                          aaaGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAACACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGa                                                                                        <  2:1209091/150‑1 (MQ=255)
                                                           aaGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAg                                                                                       <  2:1162181/150‑1 (MQ=255)
                                                             gATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCt                                                                                     <  2:1910705/150‑1 (MQ=255)
                                                                gCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGCTGCGCTggcgcaacgc                                                                                                                                        >  2:2110937/1‑96 (MQ=255)
                                                                gCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGCTGCGCTggcgcaacgc                                                                                                                                        <  1:2110937/96‑1 (MQ=255)
                                                                 cTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTCCACGACGAAGCCGAAGGCCGCGAGCTGAAc                                                                                 <  1:2246061/150‑1 (MQ=255)
                                                                 cTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTAGTCGACGAAGCCGAAGGCCGCGAGCTGAAc                                                                                 <  2:385861/150‑1 (MQ=255)
                                                                 cTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGAAGATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAAc                                                                                 <  1:2224522/150‑1 (MQ=255)
                                                                 cTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTCCTGCCACCATTGG‑G‑TGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAAc                                                                                 <  1:2097832/150‑1 (MQ=255)
                                                                  ttGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAAcc                                                                                >  2:2040397/1‑150 (MQ=255)
                                                                  ttGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAAcc                                                                                >  2:2040654/1‑150 (MQ=255)
                                                                  ttGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAAcc                                                                                >  2:2184815/1‑150 (MQ=255)
                                                                   tGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACca                                                                               <  2:621309/150‑1 (MQ=255)
                                                                    gAACTTGATATCCAACGGGCGCCGGACGCTGCCACTACAGAAGACGCCTCCTTTCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCCCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACcac                                                                              >  2:1836259/1‑150 (MQ=255)
                                                                    gAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACcac                                                                              >  2:1963699/1‑150 (MQ=255)
                                                                       cTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACAcc                                                                           <  1:2007645/150‑1 (MQ=255)
                                                                         tGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTa                                                                         <  1:1301494/150‑1 (MQ=255)
                                                                          gATACCCAACGGGCGTCGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCTAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTAc                                                                        <  2:174609/150‑1 (MQ=255)
                                                                                cAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAg                                                                                               <  1:387983/121‑1 (MQ=255)
                                                                                cAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAg                                                                                               >  2:387983/1‑121 (MQ=255)
                                                                                cAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGcac                                                                  >  1:260122/1‑150 (MQ=255)
                                                                                 aaCGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGcaca                                                                 >  2:1836617/1‑150 (MQ=255)
                                                                                  aCGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACaa                                                                <  1:2040397/150‑1 (MQ=255)
                                                                                  aCGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACaa                                                                <  1:2040654/150‑1 (MQ=255)
                                                                                   cGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAg                                                               <  1:1774883/150‑1 (MQ=255)
                                                                                        cACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTa                                                          >  1:1379807/1‑150 (MQ=255)
                                                                                             aTGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGa                                                     <  1:774812/150‑1 (MQ=255)
                                                                                                 tgcCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCaa                                                 >  1:1599872/1‑150 (MQ=255)
                                                                                                 tgcCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCaa                                                 >  2:426145/1‑150 (MQ=255)
                                                                                                   ccACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAAtg                                               <  2:1579864/150‑1 (MQ=255)
                                                                                                    cACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAAtgt                                              <  2:1100829/150‑1 (MQ=255)
                                                                                                    cACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAAGGGCCACACGCCGAGTCCACCAAGCCGATGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAAtgt                                              >  1:781581/1‑150 (MQ=255)
                                                                                                       tCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGtgtt                                           >  1:1813839/1‑150 (MQ=255)
                                                                                                        ccAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGtgttg                                          <  1:838488/150‑1 (MQ=255)
                                                                                                         cAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGtgttgt                                         >  1:1403048/1‑150 (MQ=255)
                                                                                                          agaagaCGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGtgtt                                           >  1:1382738/1‑147 (MQ=255)
                                                                                                          agaagaCGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGtgtt                                           <  2:1382738/147‑1 (MQ=255)
                                                                                                          agaagaCGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTc                                        <  2:1661082/150‑1 (MQ=255)
                                                                                                           gaagaCGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCg                                       >  2:1484116/1‑150 (MQ=255)
                                                                                                             agaCGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGtt                                     >  1:833586/1‑150 (MQ=255)
                                                                                                                cgccgcCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTccc                                  <  1:1389407/150‑1 (MQ=255)
                                                                                                                               cTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGACGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGCGCCCGa                   >  1:650146/1‑150 (MQ=255)
                                                                                                                               cTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGa                   <  2:756563/150‑1 (MQ=255)
                                                                                                                                   tGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGAc               <  1:518619/150‑1 (MQ=255)
                                                                                                                                   tGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGAc               <  1:1963699/150‑1 (MQ=255)
                                                                                                                                      gA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGACCTg            >  2:315846/1‑150 (MQ=255)
                                                                                                                                       a‑‑aTTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGACCTGc           >  1:38041/1‑150 (MQ=255)
                                                                                                                                              gCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGACCTGCTCGat      >  2:552646/1‑150 (MQ=255)
                                                                                                                                              gCGTTCCGCAAGCCCAGCGCCGACTCTGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCTGCGAGGTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGACCTGCTCGat      <  1:654266/150‑1 (MQ=255)
                                                                                                                                                gTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGACCTGCTCGatat    >  2:70977/1‑150 (MQ=255)
                                                                                                                                                gTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGACCTGCTCGatat    >  1:1706485/1‑150 (MQ=255)
                                                                                                                                                gTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGACCTGCTCGatat    >  1:1693948/1‑150 (MQ=255)
                                                                                                                                                 gtccgcCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGACCTGCTCGATATc   >  2:236095/4‑150 (MQ=255)
                                                                                                                                                  tGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGACCTGCTCGATATcc  <  1:2184815/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                
TATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGA‑‑ATTGGCGTTGCGCCAGCGCAGCGCCGACTCGGAAATGACCATTCGCCTGGTCGACGAAGCCGAAGGCCGCGAGCTGAACCACACCTACCGGCACAAGGACTACGCGACCAATGTGTTGTCGTTCCCGGCCGATGTGCCCGATGACCTGCTCGATATCC  >  NC_002947/5450109‑5450402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑