| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,701,283 | C→G | 5.4% | intergenic (+25/‑38) | phaA → / → phaB | poly(3‑hydroxyalkanoate) polymerase/poly(3‑hydroxyalkanoate) depolymerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,701,283 | 0 | C | G | 5.4% | 363.8 / 9.3 | 112 | intergenic (+25/‑38) | phaA/phaB | poly(3‑hydroxyalkanoate) polymerase/poly(3‑hydroxyalkanoate) depolymerase |
| Reads supporting (aligned to +/- strand): ref base C (49/57); new base G (3/3); total (52/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GGTTGCACTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTAACGGCAT > NC_002947/5701134‑5701430 | ggtTGCACTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCAc < 2:705901/150‑1 (MQ=255) aCTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGcc > 2:491501/1‑150 (MQ=255) tGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCTACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCAc < 1:1235455/150‑1 (MQ=255) tGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCAc < 2:494367/150‑1 (MQ=255) tGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCAc > 1:470773/1‑150 (MQ=255) gCAGGGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACgg > 1:1627516/1‑150 (MQ=255) gCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCCGGCCCCTACGTTCACGAGCCTCGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCAccg > 2:741771/1‑148 (MQ=255) agagTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGgtg > 2:1194939/1‑150 (MQ=255) gagTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGgtgt > 1:1648082/1‑150 (MQ=255) gagTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGgtgt < 2:2179166/150‑1 (MQ=255) ttGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCCGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTa < 2:1247617/150‑1 (MQ=255) ttGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTa < 1:1086051/150‑1 (MQ=255) ttGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTa < 1:491501/150‑1 (MQ=255) tGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGCTTTCTGCTCAGGCACCTACGTGTACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTAc < 1:2100183/150‑1 (MQ=255) ggCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACt < 1:386026/150‑1 (MQ=255) gggCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCAc < 1:1083296/150‑1 (MQ=255) gAGCGTGCCGGCGCGCTGACAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGGGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCaa < 2:163636/150‑1 (MQ=255) gAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCaa > 2:2355462/1‑150 (MQ=255) aGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAag < 1:1358019/150‑1 (MQ=255) gCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAag‑a < 1:1194939/150‑1 (MQ=255) gCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCa > 1:1256500/1‑150 (MQ=255) cGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACg < 1:1674311/150‑1 (MQ=255) gcgcTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCa > 1:764489/1‑150 (MQ=255) cgcTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCAt < 2:470773/150‑1 (MQ=255) gcTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGgcgct > 2:1011674/1‑115 (MQ=255) gcTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGgcgct < 1:1011674/116‑2 (MQ=255) cTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGc < 1:1085807/150‑1 (MQ=255) tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGcc > 1:249653/1‑150 (MQ=255) tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCAc < 2:1646113/116‑1 (MQ=255) tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCAc > 1:1646113/1‑116 (MQ=255) aGGCACCGACTCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAAcc > 2:228453/1‑150 (MQ=255) ggCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAAccc < 1:1877503/150‑1 (MQ=255) aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGttt < 2:1594412/113‑1 (MQ=255) aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGttt > 1:1594412/1‑113 (MQ=255) aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACtt > 2:1598833/1‑117 (MQ=255) aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCAc > 1:888526/1‑120 (MQ=255) aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCAc < 2:888526/120‑1 (MQ=255) aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCtat > 1:499300/1‑150 (MQ=255) aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCa < 1:1878835/105‑1 (MQ=255) aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCa > 2:1878835/1‑105 (MQ=255) ccGCCTGGGCAACCGTACCTATGCCGCCGGCGAATCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTc > 1:268489/1‑150 (MQ=255) ccGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAAc > 1:1052020/1‑139 (MQ=255) ccGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAAc < 2:1052020/139‑1 (MQ=255) ccGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTc > 2:1129012/1‑150 (MQ=255) cGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCa < 2:89763/150‑1 (MQ=255) cGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAACCTGGCCAGT‑CATGCCGCAACCCTATATTTTCa < 1:1871759/150‑1 (MQ=255) aCCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGa < 1:1735753/150‑1 (MQ=255) gTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCt < 1:741771/150‑1 (MQ=255) tACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTg < 2:499300/150‑1 (MQ=255) tACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTg < 1:1780869/150‑1 (MQ=255) tACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTg < 2:764489/150‑1 (MQ=255) ccTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGa < 1:2175314/150‑1 (MQ=255) aTGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCAt < 2:1220893/111‑1 (MQ=255) aTGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCAt > 1:1220893/1‑111 (MQ=255) tGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAAc > 2:1806360/1‑150 (MQ=255) ccgccgGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCa < 2:464920/150‑1 (MQ=255) ccgccgGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCa < 1:2322316/150‑1 (MQ=255) cgccgccgcAGCCTACCCCGGCACCTACGTTCACCAGCGTTGAGCTACAGCGAAGCGGGGTCCTGCACGGCGCCACGGTGTTTACTTTACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAg < 2:572053/150‑1 (MQ=255) ccgGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCaa > 2:2179058/1‑92 (MQ=255) ccgGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCaa < 1:2179058/92‑1 (MQ=255) cGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATc < 1:2355462/150‑1 (MQ=255) gAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATcc > 1:1788742/1‑150 (MQ=255) gAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATcc > 2:1997372/1‑150 (MQ=255) aaGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCg > 1:1856468/1‑150 (MQ=255) gCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCa > 2:1045971/1‑150 (MQ=255) cTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCAc > 2:69948/1‑89 (MQ=255) cTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCAc < 1:69948/89‑1 (MQ=255) ccccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGGCAACCAGTCCATCCGCACCGc < 1:2075689/150‑1 (MQ=255) ccccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGc > 1:2016525/1‑150 (MQ=255) cccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCt < 2:1856468/150‑1 (MQ=255) ccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGt < 2:1585180/135‑1 (MQ=255) ccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGt > 1:1585180/1‑135 (MQ=255) ggCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTc < 2:1648082/150‑1 (MQ=255) ggCACCTACGTTCACGACCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTc < 1:1002319/150‑1 (MQ=255) aCCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCg > 1:974078/1‑111 (MQ=255) cTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTg < 2:554845/142‑1 (MQ=255) cTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTg > 1:554845/1‑142 (MQ=255) tACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGa < 1:249345/105‑1 (MQ=255) tACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGa > 2:249345/1‑105 (MQ=255) gTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCa > 2:533549/1‑150 (MQ=255) ttCACGAGCGTTGAGTTACAGCGCTGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCaa < 1:120806/150‑1 (MQ=255) aCGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGcc > 1:1287069/1‑150 (MQ=255) gAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGc > 1:625636/1‑150 (MQ=255) gAGCGTTGAGTTACAACGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCAACCAAC‑AGTCACGTGCACCACGCAACCCCATACTTTCAGGACAGTCGCGCTGGCAAACCAGTCCATCCGCACCGCTGTTCGCACCGGCAAGCCGc > 2:109731/1‑150 (MQ=255) gCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCAc < 1:1606725/150‑1 (MQ=255) gTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCt < 1:1129012/150‑1 (MQ=255) aGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAAcc > 1:1918638/1‑74 (MQ=255) aGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAAcc < 2:1918638/74‑1 (MQ=255) ttACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATc > 2:1196187/1‑112 (MQ=255) ttACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGcc > 1:2007132/1‑150 (MQ=255) gcgcCGTGGCGGCCTGCGCGGCGCCACGGTGTTGACGTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCGCCTGACGCCGTTGc < 2:497434/150‑1 (MQ=255) gcCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTg > 1:2169539/1‑150 (MQ=255) accGTGGCGCCGTGCAGGCCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTg > 1:2181763/2‑150 (MQ=255) gTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATc > 1:1620188/1‑150 (MQ=255) tGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCCCTGGCCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAAACGCAACTCACGCCGTTGCTGATCt > 1:1955862/1‑150 (MQ=255) ggcCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTaa > 2:2220714/1‑150 (MQ=255) ggcCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTaa < 2:191750/150‑1 (MQ=255) tGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTGACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTAACGGc > 2:606412/1‑150 (MQ=255) tGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCGACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAGGCCGCAc < 2:96518/123‑1 (MQ=255) cACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTAACGGCAt < 2:2003585/150‑1 (MQ=255) | GGTTGCACTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTAACGGCAT > NC_002947/5701134‑5701430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |