Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	524,339	A→G	12.1%	intergenic (+203/‑613)	tyrS → / → PP_16SC	tyrosine‑‑tRNA ligase/16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	524,339	0	A	G	12.1%	166.6 / 12.8	66	intergenic (+203/‑613)	tyrS/PP_16SC	tyrosine‑‑tRNA ligase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (32/26);  new base G (3/5);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

TTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATG  >  NC_002947/524196‑524484                                                                                                                                                 |                                                                                                                                                  tttGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAg                                                                                                                                             >  2:1195879/1‑150 (MQ=255)     gggTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGcgcc                                                                                                                                         <  1:1195879/150‑1 (MQ=255)     gggTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGcgcc                                                                                                                                         >  1:619295/1‑150 (MQ=255)       gTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGcgccgc                                                                                                                                       >  2:1452477/1‑150 (MQ=255)             tggtTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCgcgggc                                                                                                                                 <  2:619295/150‑1 (MQ=255)               gtTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCgcgggcgg                                                                                                                               >  2:1682328/1‑150 (MQ=255)               gtTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCgcgggcgg                                                                                                                               >  1:1404015/1‑150 (MQ=255)                ttGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGggcggc                                                                                                                              <  1:140985/150‑1 (MQ=255)                  gCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAGACCGAGCGCCGCCCGCGCGgcgc                                                                                                                            <  2:182451/150‑1 (MQ=255)                    tgttgtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTc                                                                                                                          >  2:1496814/1‑150 (MQ=255)                    tgttgtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTc                                                                                                                          >  2:1273997/1‑150 (MQ=255)                      ttgtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATTTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCCCGCGCGGCGCTCgg                                                                                                                        <  1:1943866/150‑1 (MQ=255)                        gtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGtc                                                                                                                      <  1:1682328/150‑1 (MQ=255)                           gggggagggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAGACCGAGCGCCGCCCGCGCGGCGCTCg                                                                                                                         <  2:2045730/144‑1 (MQ=255)                           gggggagggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAGACCGAGCGCCGCCCGCGCGGCGCTCg                                                                                                                         >  1:2045730/1‑144 (MQ=255)                              ggagggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCGCCGCCTATGaaa                                                                                                                                                  >  2:992394/1‑116 (MQ=255)                              ggagggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCGCCGCCTATGaaa                                                                                                                                                  <  1:992394/116‑1 (MQ=255)                              ggagggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAGACCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAg                                                                                                                <  1:249046/150‑1 (MQ=25)                               gagggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAgg                                                                                                               <  1:1452477/150‑1 (MQ=255)                                  ggggCTGCTTTGAGAGGGTGTTGGTTGTGAGATTTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATACGCGGCCTATAAAACGGTCCGCCTCGCGGGCGGCGCTCGGTCTCATAGgcgc                                                                                                            >  2:1729043/1‑150 (MQ=21)                                   gggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCt                                                                                                           <  1:1729043/150‑1 (MQ=255)                                    gtcTGTTTTGAGGGGGTGTTGGCGGTCAGATGCGTCCGCGACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCCCCGCGCGGGCGGCCCTCGGTCTCACAGgcgcgg                                                                                                          >  2:41171/3‑148 (MQ=25)                                    ggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGgcg                                                                                                             <  2:667835/147‑1 (MQ=255)                                    ggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGgcg                                                                                                             >  1:667835/1‑147 (MQ=255)                                       tGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGaaa                                                                                                       >  2:1585390/1‑150 (MQ=255)                                              agagGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCgcgggcg                                                                                                                                >  2:549019/1‑118 (MQ=255)                                              agagGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCgcgggcg                                                                                                                                <  1:549019/118‑1 (MQ=255)                                                  ggTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCg                                                                                            >  2:2265061/1‑150 (MQ=255)                                                  ggTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGAGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCg                                                                                            >  2:198987/1‑150 (MQ=255)                                                   gtgtTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGa                                                                                           >  1:1973670/1‑150 (MQ=255)                                                      ttGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAaca                                                                                        >  1:345127/1‑150 (MQ=255)                                                         gCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACtt                                                                                     <  1:1724781/150‑1 (MQ=255)                                                           ggTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTgg                                                                                   <  2:67586/150‑1 (MQ=255)                                                              gagaTGTGTCCGCCACAACCCTTGCAGCGCCTGTGATATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAg                                                                                >  2:477077/1‑150 (MQ=255)                                                                      tCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATa                                                                        >  2:1360336/1‑150 (MQ=255)                                                                         gCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCACATCTGAAGCGCT‑GCCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGACACTGTCGAGGCGAACACTTGGCAGCCTCGATTCgg                                                                     >  2:1791813/1‑150 (MQ=21)                                                                                 ccTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTc                                                                                                  >  1:352987/1‑113 (MQ=255)                                                                                 ccTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTc                                                                                                  <  2:352987/113‑1 (MQ=255)                                                                                    tGCAGCGCCTGTGAGATCGCGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCt                                                          <  2:1163888/150‑1 (MQ=255)                                                                                    tGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCt                                                          <  1:3866/150‑1 (MQ=255)                                                                                       aGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTcccc                                                           >  2:406443/1‑146 (MQ=255)                                                                                       aGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTcccc                                                           <  1:406443/146‑1 (MQ=255)                                                                                       aGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTtat                                                       >  2:815894/1‑150 (MQ=255)                                                                                           ccTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAGACCGAGCGCCGCCCGCGCGGCGCTCg                                                                                                                         <  2:2032202/80‑1 (MQ=25)                                                                                           ccTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAGACCGAGCGCCGCCCGCGCGGCGCTCg                                                                                                                         >  1:2032202/1‑80 (MQ=25)                                                                                            cTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGaaa                                                                                                                                                  <  1:724467/54‑1 (MQ=255)                                                                                            cTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGaaa                                                                                                                                                  >  2:724467/1‑54 (MQ=255)                                                                                                  gaTCGAGCGCCGCCCGCGTCCGTCT‑TGAGACATTCACCGCCTATGAAGCCGAGCGCCGCGCGCGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCCCTTTc                                            <  1:1479151/150‑1 (MQ=255)                                                                                                  gaTCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCCCCGAGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTc                                            >  2:934964/1‑150 (MQ=255)                                                                                                   aTCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCt                                           >  1:2219591/1‑150 (MQ=255)                                                                                                              cccgcGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAg                                <  1:2265061/150‑1 (MQ=255)                                                                                                                 gcgggcGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAACCTGTACTGCCGAACACTTGGCAGCCTCGATACGGTGTTTTCCCATTCTATGTCACTTGCTGGAAATTAAAGttt                             >  1:418977/6‑150 (MQ=18)                                                                                                                 gcgTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTAGAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGtt                             <  1:1496814/150‑1 (MQ=255)                                                                                                                    tCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGAc                          <  1:584649/150‑1 (MQ=255)                                                                                                                    ggcGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGAc                          <  1:1940705/148‑1 (MQ=255)                                                                                                                              agaCATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCtt                                          >  2:607702/1‑125 (MQ=255)                                                                                                                              agaCATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCtt                                          <  1:607702/125‑1 (MQ=255)                                                                                                                                aCATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGaa               <  1:41171/150‑1 (MQ=255)                                                                                                                                  aTTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATc             >  2:19456/1‑150 (MQ=255)                                                                                                                                   ttCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATcc            >  1:1332296/1‑150 (MQ=255)                                                                                                                                    tCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATccc           <  1:1585390/150‑1 (MQ=255)                                                                                                                                    tCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATccc           >  1:144072/1‑150 (MQ=255)                                                                                                                                      aCCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTtat                                                       >  2:168513/1‑104 (MQ=255)                                                                                                                                      aCCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTtat                                                       <  1:168513/104‑1 (MQ=255)                                                                                                                                      aCCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCt         >  2:1662156/1‑150 (MQ=255)                                                                                                                                       gcGCCTATGAGACCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGGTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATa                                                                        >  2:2177372/2‑86 (MQ=11)                                                                                                                                       gcGCCTATGAGACCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGGTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATa                                                                        <  1:2177372/85‑1 (MQ=11)                                                                                                                                           cTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATaa    <  1:1360336/150‑1 (MQ=255)                                                                                                                                             aTGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATg  >  2:359341/1‑150 (MQ=255)                                                                                                                                             aTGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATg  <  1:815894/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  TTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCT‑TGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATG  >  NC_002947/524196‑524484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑