BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,741,711	C→T	5.3%	A167T (GCC→ACC)	PP_5039 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,741,711	0	C	T	5.3%	363.0 / 4.5	120	A167T (GCC→ACC)	PP_5039	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (51/60); new base T (2/5); total (53/67)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

AATGGGAGCGGCCTTGCGTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCA  >  NC_002947/5741563‑5741857

aaTGGGAGCGGCCTTGCGTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCa                                                                                                                                                   >  1:286411/1‑150 (MQ=255)

 aTGGGAGCGGCCTTGCGTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCaa                                                                                                                                                  <  1:1476031/150‑1 (MQ=255)

         ggCCTTGCGTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGc                                                                                                                                          >  2:996398/1‑150 (MQ=255)

          gCCTTGCGTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCt                                                                                                                                         >  1:1539852/1‑150 (MQ=255)

             ttGCGTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGTAATTGCCCGCttcg                                                                                                                                      <  2:766354/150‑4 (MQ=255)

             ttGCGTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGt                                                                                                                                      >  2:787688/1‑150 (MQ=255)

              tGCGTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGtg                                                                                                                                     <  1:1768506/150‑1 (MQ=255)

              tGCGTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCCCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGtg                                                                                                                                     <  2:198973/150‑1 (MQ=255)

                 gTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCATGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGtgctg                                                                                                                                  >  2:2152131/1‑150 (MQ=255)

                 gTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGtgctg                                                                                                                                  >  1:593317/1‑150 (MQ=255)

                 gTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGtgctg                                                                                                                                  >  2:2346969/1‑150 (MQ=255)

                     cgATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCg                                                                                                                              <  1:1351879/150‑1 (MQ=255)

                     cgATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCg                                                                                                                              <  2:1079209/150‑1 (MQ=255)

                      gATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCTCGGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCgg                                                                                                                             >  1:1373512/1‑150 (MQ=255)

                      gATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGTTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCgg                                                                                                                             <  2:593317/150‑1 (MQ=255)

                      gATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCgg                                                                                                                             <  2:45573/150‑1 (MQ=255)

                       aTGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGCCTCGCGAACAAAGTCCATCTCTTCGGGGGGGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGc                                                                                                                            >  1:45573/1‑150 (MQ=255)

                         gCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCAt                                                                                                                          >  2:1966074/1‑150 (MQ=255)

                         gCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTC‑GCCGCTCCAGCACCAGGTTGCGGTGCGTGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATa                                                                                                                         <  2:197611/150‑1 (MQ=255)

                            tGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGt                                                                                                                       >  2:2375958/1‑150 (MQ=255)

                            tGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGt                                                                                                                       >  2:1424927/1‑150 (MQ=255)

                            tGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCAGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGt                                                                                                                       >  2:1656539/1‑150 (MQ=255)

                             gCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTc                                                                                                                      <  2:1539852/150‑1 (MQ=255)

                               acaGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGa                                                                                                                    <  2:286411/150‑1 (MQ=255)

                               acaGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGa                                                                                                                    <  2:2207182/150‑1 (MQ=255)

                               acaGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGa                                                                                                                    <  1:1851082/150‑1 (MQ=255)

                               acaGCAGGCCCCTTGGGCCGCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCACATCTTGATCAGCGTACCACCCCGCCAGCACCAGGTTCCGCTGCGGGAAGAGCGCGACGGGGGGAATGACCCGCTGGTGCTGCTCGGCATAGTCGa                                                                                                                    <  1:551306/150‑1 (MQ=255)

                               acaGCAGCCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAACTTCTTCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGa                                                                                                                    <  1:1352303/150‑1 (MQ=255)

                                 agcagGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAgg                                                                                                                  >  1:1154443/1‑150 (MQ=255)

                                   cagGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGAACGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGaa                                                                                                                <  2:341123/150‑1 (MQ=255)

                                    agGCCCCTGGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAg                                                                                                               <  2:996270/150‑1 (MQ=255)

                                     ggCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGc                                                                                                              <  2:1373512/150‑1 (MQ=255)

                                      gCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGcc                                                                                                             >  1:317605/1‑150 (MQ=255)

                                       ccccTTGGGCCTCAGAACCGGGACCCGGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCt                                                                                                            >  2:2088790/1‑150 (MQ=255)

                                        cccTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCATGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCtt                                                                                                           <  1:2152131/150‑1 (MQ=255)

                                        cccTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGTAATTGCCCGCttcgg                                                                                                                                     <  1:1923956/124‑5 (MQ=255)

                                         ccTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTc                                                                                                          >  1:808942/1‑150 (MQ=255)

                                         ccTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCACGTTGCGGTGCGGGAAGAGCGCCGTTTTCGTGATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTc                                                                                                          <  1:1622875/150‑1 (MQ=255)

                                          cTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCa                                                                                                         <  2:1564717/150‑1 (MQ=255)

                                                ccTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGc                                                                                                              <  2:1942186/139‑1 (MQ=255)

                                                ccTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGc                                                                                                              >  1:1942186/1‑139 (MQ=255)

                                                ccTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGc                                                                                                   <  1:2346969/150‑1 (MQ=255)

                                                ccTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGc                                                                                                   <  1:1098044/150‑1 (MQ=255)

                                                 cTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGTAATTGCCCGCttcgg                                                                                                                                     <  2:1148264/115‑5 (MQ=255)

                                                 cTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGTAATTGCCCGCttcgg                                                                                                                                     >  1:1148264/1‑111 (MQ=255)

                                                 cTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGcc                                                                                                  >  1:1139106/1‑150 (MQ=255)

                                                  tCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGTGAGCCTTCAAACAGCcg                                                                                                 >  1:736551/1‑150 (MQ=255)

                                                  tCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCcg                                                                                                 <  1:1966074/150‑1 (MQ=255)

                                                     gAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGGCTTCAACCAGCcggcg                                                                                              >  1:177980/1‑150 (MQ=255)

                                                      aaCCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCcggcgg                                                                                             >  2:115784/1‑150 (MQ=255)

                                                        ccGGGACCCGGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGtt                                                                                           <  1:2088790/150‑1 (MQ=255)

                                                          gggACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGtt                                                                                           <  1:974964/148‑1 (MQ=255)

                                                          gggACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGtt                                                                                           >  2:974964/1‑148 (MQ=255)

                                                               ccAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCg                                                                                    >  1:1512701/1‑150 (MQ=255)

                                                                     tCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGt                                                                              <  1:804660/150‑1 (MQ=255)

                                                                       cAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTc                                                                            <  2:93740/150‑1 (MQ=255)

                                                                       cAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTc                                                                            >  2:1492897/1‑150 (MQ=255)

                                                                        aGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCg                                                                           >  1:1483747/1‑150 (MQ=255)

                                                                                 tCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCagcagc                                                                  >  2:204592/1‑150 (MQ=255)

                                                                                 tCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCagcagc                                                                  >  1:1907675/1‑150 (MQ=255)

                                                                                    aTCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCAtt                                                               <  2:1699413/150‑1 (MQ=255)

                                                                                    aTCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCAtt                                                               >  2:1781226/1‑150 (MQ=255)

                                                                                      ctctTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTc                                                                         >  2:686726/1‑138 (MQ=255)

                                                                                      ctctTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTc                                                                         <  1:686726/138‑1 (MQ=255)

                                                                                       tctTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGa                                                            <  1:691508/150‑1 (MQ=255)

                                                                                         ttCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGTCTCCTGCTGTTCGTCCAGCAGCATTCGATa                                                          <  1:1873490/150‑1 (MQ=255)

                                                                                         ttCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATa                                                          >  1:1901769/1‑150 (MQ=255)

                                                                                          tCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATaa                                                         <  2:1154443/150‑1 (MQ=255)

                                                                                               ggTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTc                                                    >  1:1243448/1‑150 (MQ=255)

                                                                                                   cTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACg                                                <  2:1243448/150‑1 (MQ=255)

                                                                                                      ggCCGCTCCAGCACCAGGTTGCGGGGCGGGAGGCGCCCGAAGCGGGGAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACGGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGcac                                             <  1:2365523/150‑1 (MQ=255)

                                                                                                        ccGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAg                                           <  1:1883678/150‑1 (MQ=255)

                                                                                                            tCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCg                                                                                    >  1:1725590/1‑105 (MQ=255)

                                                                                                            tCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCg                                                                                    <  2:1725590/105‑1 (MQ=255)

                                                                                                            tCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTc                                       >  2:1974730/1‑150 (MQ=255)

                                                                                                              cAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTg                                     >  1:2089133/1‑150 (MQ=255)

                                                                                                               aGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATaa                                                         >  2:1283480/1‑129 (MQ=255)

                                                                                                               aGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATaa                                                         <  1:1283480/129‑1 (MQ=255)

                                                                                                               aGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGc                                    <  1:204592/150‑1 (MQ=255)

                                                                                                               aGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGc                                    <  2:736551/150‑1 (MQ=255)

                                                                                                               aGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGc                                    <  1:1781226/150‑1 (MQ=255)

                                                                                                               aGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGc                                    <  2:1293905/150‑1 (MQ=255)

                                                                                                                 cACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCa                                  <  1:2375958/150‑1 (MQ=255)

                                                                                                                  aCCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGGTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAg                                 >  1:879737/1‑150 (MQ=255)

                                                                                                                  aCCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAg                                 >  1:1476957/1‑150 (MQ=255)

                                                                                                                  aCCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAg                                 <  2:1476957/150‑1 (MQ=255)

                                                                                                                    cAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTc                               >  1:2063032/1‑150 (MQ=255)

                                                                                                                     aGGCTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTcc                              <  1:408419/150‑1 (MQ=255)

                                                                                                                      ggTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCa                             <  1:996398/150‑1 (MQ=255)

                                                                                                                            ggttcggGAAGCGCCCGAAGCGGGTAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACtt                       <  1:2040929/150‑1 (MQ=255)

                                                                                                                                cggGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGc                                    <  2:1895649/133‑1 (MQ=255)

                                                                                                                                cggGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGc                   <  2:2096617/150‑1 (MQ=255)

                                                                                                                                   gAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATg                <  2:2135154/150‑1 (MQ=255)

                                                                                                                                    aaGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGc               <  2:45675/150‑1 (MQ=255)

                                                                                                                                      gcgcCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTc             <  2:785134/150‑1 (MQ=255)

                                                                                                                                       cgcCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTcc            <  1:115784/150‑1 (MQ=255)

                                                                                                                                         cccGAAGCGGGCAATTACCCGCTGGTGCTGCTAGGCATAGGCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGACTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAg          >  1:1857186/1‑150 (MQ=255)

                                                                                                                                                 gggCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACcagca  <  2:1139106/150‑1 (MQ=255)

                                                                                                                                                 gggCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACca     <  2:2147644/147‑1 (MQ=255)

                                                                                                                                                 gggCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACca     >  1:2147644/1‑147 (MQ=255)

AATGGGAGCGGCCTTGCGTCGCGATGCCTGCACAGCAGGCCCCTTGGGCCTCAGAACCGGGACCCAGGCTCCAGCAAAAAGTCCATCTCTTCGCTGGTGCTCGGCCGCTCCAGCACCAGGTTGCGGTGCGGGAAGCGCCCGAAGCGGGCAATTACCCGCTGGTGCTGCTCGGCATAGTCGAGGAAGCCTTCAAACAGCCGGCGGTTGGCCTCGGGCTGTTCGTCCAGCAGCATTCGATAACGCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCA  >  NC_002947/5741563‑5741857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`