Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,743,906 G→T 6.9% A672E (GCG→GAG)  glgP ← glycogen phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,743,9060GT 6.9% 269.6 / 6.1 86A672E (GCG→GAG) glgPglycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (38/42);  new base T (4/2);  total (42/44)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

GCGGCTATGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGC  >  NC_002947/5743759‑5744050
                                                                                                                                                   |                                                                                                                                                
gCGGCTATGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCa                                                                                                                                                >  2:949992/1‑150 (MQ=255)
 cGGCTATGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCAc                                                                                                                                               <  1:724585/150‑1 (MQ=255)
 aggCTATGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCAc                                                                                                                                               <  2:1187603/149‑1 (MQ=255)
    cTATGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCAt                                                                                                                                            >  1:1065330/1‑150 (MQ=255)
     tATGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGCGCTCACCAtt                                                                                                                                           <  2:550587/150‑1 (MQ=255)
       tGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGa                                                                                                                                         <  1:431696/150‑1 (MQ=255)
         gcagcaTTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAgg                                                                                                                                       <  2:1964716/150‑1 (MQ=255)
             caTTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGa                                                                                                                                   >  2:2158361/1‑150 (MQ=255)
               tttgcgcTAAAATCACCGGCACGCTTGCGCGCTTCGACCTTCTGCGAGGTCAAGCCAAAGATGAACATTTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAAc                                                                                                                                 <  2:1036812/147‑1 (MQ=255)
                    gcCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCAt                                                                                                                            <  2:1065330/150‑1 (MQ=255)
                    gcCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCAt                                                                                                                            <  1:1321073/150‑1 (MQ=255)
                    gcCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCAt                                                                                                                            >  1:1793802/1‑150 (MQ=255)
                     ccAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTg                                                                                                                                          <  2:1825010/135‑1 (MQ=255)
                     ccAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTg                                                                                                                                          >  1:1825010/1‑135 (MQ=255)
                     ccAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATg                                                                                                                           <  1:40430/150‑1 (MQ=255)
                         aaTCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCCAAGCTGAACATGTCGTCGGAACCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGc                                                                                                                       <  1:1250646/150‑1 (MQ=255)
                          aTCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGACAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCt                                                                                                                      <  1:2295453/150‑1 (MQ=255)
                           tCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTg                                                                                                                     <  2:454366/150‑1 (MQ=255)
                            cACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTgg                                                                                                                    >  2:2404940/1‑150 (MQ=255)
                               cGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGa                                                                                                                                         <  1:801830/126‑1 (MQ=255)
                               cGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGa                                                                                                                                         >  2:801830/1‑126 (MQ=255)
                               cGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGa                                                                                                                                         >  2:1183042/1‑126 (MQ=255)
                               cGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGa                                                                                                                                         <  1:1183042/126‑1 (MQ=255)
                                   aCGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGa                                                                                                             >  2:771073/1‑150 (MQ=255)
                                    cGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGaa                                                                                                            <  1:963662/150‑1 (MQ=255)
                                         gcgcgcTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTc                                                                                                       <  1:1002995/150‑1 (MQ=255)
                                             gcTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTaa                                                                                                   >  2:623202/1‑150 (MQ=255)
                                               ttCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAAcc                                                                                                 >  1:700863/1‑150 (MQ=255)
                                                     ctgctgCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGt                                                                                           >  1:1445186/1‑150 (MQ=255)
                                                        ctgcGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGa                                                                                        <  2:1100300/150‑1 (MQ=255)
                                                         tgcGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTc                                                                                                       >  1:1899339/1‑134 (MQ=255)
                                                         tgcGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTc                                                                                                       <  2:1899339/134‑1 (MQ=255)
                                                           cGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGatat                                                                                     <  2:1962478/150‑1 (MQ=255)
                                                              ggTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTg                                                                                  >  1:2092008/1‑150 (MQ=255)
                                                               gTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCTGGGTCCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGc                                                                                 >  2:812780/1‑150 (MQ=255)
                                                               gTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGt                                                                                           >  2:217034/1‑140 (MQ=255)
                                                               gTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGt                                                                                           <  1:217034/140‑1 (MQ=255)
                                                                 cAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTc                                                                               <  2:1445186/150‑1 (MQ=255)
                                                                  aGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCCCg                                                                              <  1:2404940/150‑1 (MQ=255)
                                                                          gATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGc                                                                                                                                      >  2:266065/1‑86 (MQ=255)
                                                                          gATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGc                                                                                                                                      <  1:266065/86‑1 (MQ=255)
                                                                           aTGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTcc                                                                     <  1:623202/150‑1 (MQ=255)
                                                                                 aTGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTc                                                                               >  2:1122361/1‑134 (MQ=255)
                                                                                 aTGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTc                                                                               <  1:1122361/134‑1 (MQ=255)
                                                                                    ttgtCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCggg                                                            >  2:71107/1‑150 (MQ=255)
                                                                                     tgtCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGga                                                           <  2:2092008/150‑1 (MQ=255)
                                                                                       tCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCt                                                                                                                      >  1:1259848/1‑89 (MQ=255)
                                                                                       tCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCt                                                                                                                      <  2:1259848/89‑1 (MQ=255)
                                                                                        cGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGgatga                                                        <  2:1793802/150‑1 (MQ=255)
                                                                                            ccccACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGga                                                           >  2:425040/1‑143 (MQ=255)
                                                                                            ccccACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGga                                                           <  1:425040/143‑1 (MQ=255)
                                                                                            ccccACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGct                                                    >  2:779140/1‑150 (MQ=255)
                                                                                             cccACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCTTTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATTTGCTCGGAAAGGTCCGCCGCCGTGATGCTGctc                                                   >  1:1098899/1‑150 (MQ=255)
                                                                                              ccACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGctct                                                  >  2:1011485/1‑150 (MQ=255)
                                                                                                  cTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCt                                                                                                                      >  2:584120/1‑78 (MQ=255)
                                                                                                  cTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCt                                                                                                                      <  1:584120/78‑1 (MQ=255)
                                                                                                  cTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGc                                              >  1:982356/1‑150 (MQ=255)
                                                                                                      tCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAgg                                          <  1:1291011/150‑1 (MQ=255)
                                                                                                       cGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGc                                         <  1:2184437/150‑1 (MQ=255)
                                                                                                        gCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGGAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCg                                                <  1:280560/142‑1 (MQ=255)
                                                                                                        gCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCg                                                >  2:280560/1‑142 (MQ=255)
                                                                                                             tctcAACGGTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGt                                   >  2:705146/1‑150 (MQ=255)
                                                                                                                 cACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTa                               <  1:1603380/150‑1 (MQ=255)
                                                                                                                    gTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGtt                            >  1:470913/1‑150 (MQ=255)
                                                                                                                       ggCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTggg                         <  1:705146/150‑1 (MQ=255)
                                                                                                                         cgcCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCa                       >  1:749607/1‑150 (MQ=255)
                                                                                                                          gcCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAg                      >  1:388842/1‑150 (MQ=255)
                                                                                                                            cATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGa                    >  1:211785/1‑150 (MQ=255)
                                                                                                                             aTCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGaa                   <  1:1011485/150‑1 (MQ=255)
                                                                                                                             aTCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGaa                   <  1:74839/150‑1 (MQ=255)
                                                                                                                              tCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTccg                                                                    >  1:1685474/1‑100 (MQ=255)
                                                                                                                              tCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTccg                                                                    <  2:1685474/100‑1 (MQ=255)
                                                                                                                              tCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAac                  >  2:1023185/1‑150 (MQ=255)
                                                                                                                                cAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAacac                <  1:949992/150‑1 (MQ=255)
                                                                                                                                    gTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTAGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACaccacc            >  2:1882748/1‑150 (MQ=255)
                                                                                                                                      ctcAATGGTGCGCTCACCATTGGGGCCGAACTTCATGTTACTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCtt          >  2:207326/3‑150 (MQ=255)
                                                                                                                                       tcAATGGTGCGCTCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTc         >  2:58049/2‑150 (MQ=255)
                                                                                                                                       tcAATGGTGCGCTCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTc         >  1:210584/2‑150 (MQ=255)
                                                                                                                                        cAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTca        >  2:1505214/1‑150 (MQ=255)
                                                                                                                                         aaTGGTGCGCTCACCATTGAGGCCGAACTTCACGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCtctc                                                 <  1:222414/108‑1 (MQ=255)
                                                                                                                                         aaTGGTGCGCTCACCATTGAGGCCGAACTTCACGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCtctc                                                 >  2:222414/1‑108 (MQ=255)
                                                                                                                                         aaTGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTccgccgccg                                                              >  2:1243805/1‑95 (MQ=255)
                                                                                                                                         aaTGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTccgccgccg                                                              <  1:1243805/95‑1 (MQ=255)
                                                                                                                                          aTGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGACAGCCTGACGATGTAGTTGGGCAGGAACACCACCTTcagc      >  2:1999939/1‑150 (MQ=255)
                                                                                                                                             gTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAgg   <  1:771073/150‑1 (MQ=255)
                                                                                                                                              tGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGc  <  2:982356/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCGGCTATGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGC  >  NC_002947/5743759‑5744050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑