Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,790,676 A→T 5.3% intergenic (‑24/+33) PP_5073 ← / ← hemE hypothetical protein/uroporphyrinogen decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,790,6760AT 5.3% 290.7 / 4.0 90intergenic (‑24/+33)PP_5073/hemEhypothetical protein/uroporphyrinogen decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (41/44);  new base T (3/2);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAAATGCGCCAGTGCTGAACAAGGGGGCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCAGTGCCGTG  >  NC_002947/5790533‑5790825
                                                                                                                                               |                                                                                                                                                     
cGCAAATGCGCCAGTGCTGAACAAGGGGGCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAg                                                                                                                                                 <  2:2185053/150‑1 (MQ=255)
       gcgcCAGTGCTGAACAAGGGGGCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCTTCGATGCCGGGtt                                                                                                                                          <  2:1436284/150‑1 (MQ=255)
       gcgcCAGTGCTGAACAAGGGGGCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCTTCGATGCCGGGtt                                                                                                                                          <  2:1951101/150‑1 (MQ=255)
              tGCTGAACAAGGGGGCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACtgtg                                                                                                                                   <  1:2001924/150‑1 (MQ=255)
                cTGAACAAGGGGGCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTgtgca                                                                                                                                 <  2:1688378/150‑1 (MQ=255)
                 tGAACAAGGGGGCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTgtgcag                                                                                                                                >  1:2124148/1‑150 (MQ=255)
                  gAACAAGGGGGCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTgtgcagt                                                                                                                               <  2:2324993/150‑1 (MQ=255)
                    aCAAGGGGGCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTgtgcagtgc                                                                                                                             <  1:410946/150‑1 (MQ=255)
                      aaGGGGGCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATGCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCAt                                                                                                                           >  1:637433/1‑150 (MQ=255)
                        gggggCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCa                                                                                                                         >  2:2366240/1‑150 (MQ=255)
                        gggggCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCa                                                                                                                         <  2:2124148/150‑1 (MQ=255)
                         ggggCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCaa                                                                                                                        <  1:914504/150‑1 (MQ=255)
                          gggCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAg                                                                                                                       >  2:1142592/1‑150 (MQ=255)
                              gAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCgg                                                                                                                                             <  1:1436983/124‑1 (MQ=255)
                              gAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCgg                                                                                                                                             >  2:1436983/1‑124 (MQ=255)
                                   tGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACtggtg                                                                                                              <  1:743116/150‑1 (MQ=255)
                                   tGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACtggtg                                                                                                              <  2:888314/150‑1 (MQ=255)
                                       ggCGAGATAACGGATTTTCATGATAATCGGGTCTTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATTGAAGCCGAGTTTACTGTGCTGTTCATCAAGTCACTggtggtgt                                                                                                          >  2:890617/1‑148 (MQ=255)
                                          gagaTAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACtggtg                                                                                                              >  2:151435/1‑143 (MQ=255)
                                          gagaTAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACtggtg                                                                                                              <  1:151435/143‑1 (MQ=255)
                                          gagaTAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTgc                                                                                                       <  1:1529279/150‑1 (MQ=255)
                                               aaCGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGa                                                                                                  >  1:1761435/1‑150 (MQ=255)
                                                aCGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACtggtg                                                                                                              >  2:242946/1‑137 (MQ=255)
                                                aCGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACtggtg                                                                                                              <  1:242946/137‑1 (MQ=255)
                                                    aTTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCAt                                                                                                                           >  2:1491077/1‑120 (MQ=255)
                                                    aTTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCAt                                                                                                                           <  1:1491077/120‑1 (MQ=255)
                                                     ttttCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTc                                                                                            <  1:2271954/150‑1 (MQ=255)
                                                          aTGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGcc                                                                                                    <  1:1779361/137‑1 (MQ=255)
                                                          aTGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGcc                                                                                                    >  2:1779361/1‑137 (MQ=255)
                                                          aTGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCa                                                                                       >  1:1313859/1‑150 (MQ=255)
                                                             aTAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGt                                                                                                                                           >  2:1803778/1‑95 (MQ=255)
                                                             aTAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGt                                                                                                                                           <  1:1803778/95‑1 (MQ=255)
                                                             aTAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACgg                                                                                    <  2:1221059/150‑1 (MQ=255)
                                                                aTCGGGTCCTGTTTCGGTTTCGATGGGATCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGt                                                                                 >  2:2240464/1‑150 (MQ=255)
                                                                aTCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGtt                                                                                                                                          >  1:1458302/1‑93 (MQ=255)
                                                                aTCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGtt                                                                                                                                          <  2:1458302/93‑1 (MQ=255)
                                                                 tCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGtt                                                                                >  1:6145/1‑150 (MQ=255)
                                                                        cTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAAt                                                                         >  1:1675087/1‑150 (MQ=255)
                                                                           tttcggtttcgATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCa                                                                      >  2:58025/1‑150 (MQ=255)
                                                                           tttcggtttcgATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCa                                                                      >  1:1243921/1‑150 (MQ=255)
                                                                            ttcggtttcgATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCAc                                                                     >  2:1149014/1‑150 (MQ=255)
                                                                                gtttcgATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCaa                                                                                                                        <  2:882626/95‑1 (MQ=255)
                                                                                gtttcgATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCaa                                                                                                                        >  1:882626/1‑95 (MQ=255)
                                                                                gtttcgATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGt                                                                                          >  1:2364677/1‑125 (MQ=255)
                                                                                gtttcgATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGt                                                                                          <  2:2364677/125‑1 (MQ=255)
                                                                                         ggACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCgg                                                        <  2:1761435/150‑1 (MQ=255)
                                                                                          gACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTtgatga                                                                           <  2:950269/130‑1 (MQ=255)
                                                                                          gACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTtgatga                                                                           >  1:950269/1‑130 (MQ=255)
                                                                                           aCCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCATGGCGTTGATGAATGCACCCGCATGTTCCGGGt                                                      >  2:1168452/1‑150 (MQ=255)
                                                                                           aCCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGt                                                      >  1:352276/1‑150 (MQ=255)
                                                                                           aCCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGt                                                      <  1:220994/150‑1 (MQ=255)
                                                                                            ccAGATTAGCGACGATCGATGAATTCAAACTTAACGTACAAAAAACCCGGCAGCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTc                                                     <  2:977751/150‑1 (MQ=255)
                                                                                             cAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCa                                                    >  2:1854247/1‑150 (MQ=255)
                                                                                              aGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCaa                                                   <  2:1243921/150‑1 (MQ=255)
                                                                                               gATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTgc                                                                                                       >  1:813079/1‑97 (MQ=255)
                                                                                               gATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTgc                                                                                                       <  2:813079/97‑1 (MQ=255)
                                                                                                    gCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACt                                                                                                                  >  2:1258273/1‑81 (MQ=255)
                                                                                                    gCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACt                                                                                                                  <  1:1258273/81‑1 (MQ=255)
                                                                                                     cgacgaTCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCgg                                            <  2:6145/150‑1 (MQ=255)
                                                                                                      gacgaTCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGc                                           >  2:1303599/1‑150 (MQ=255)
                                                                                                      gacgaTCGCAGAATTCAAACTTAACGGACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGc                                           <  1:890617/150‑1 (MQ=255)
                                                                                                        cgaTCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGt                                         <  1:58025/150‑1 (MQ=255)
                                                                                                        cgaTCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGt                                         <  1:1889362/150‑1 (MQ=255)
                                                                                                        cgaTCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGt                                         <  1:2354865/150‑1 (MQ=255)
                                                                                                               tGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCggg                                                       >  2:691070/1‑129 (MQ=255)
                                                                                                               tGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCggg                                                       <  1:691070/129‑1 (MQ=255)
                                                                                                                gAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGTGGCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGt                                 <  1:636677/150‑1 (MQ=255)
                                                                                                                 aaTTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTg                                <  2:1313859/150‑1 (MQ=255)
                                                                                                                 aaTTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTg                                <  1:2240464/150‑1 (MQ=255)
                                                                                                                   ttCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACtggtg                                                                                                              <  2:220031/70‑1 (MQ=255)
                                                                                                                   ttCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACtggtg                                                                                                              >  1:220031/1‑70 (MQ=255)
                                                                                                                   ttCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGc                              >  1:1509285/1‑150 (MQ=255)
                                                                                                                        aCTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAgg                         >  1:2384047/1‑150 (MQ=255)
                                                                                                                         cTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTCCCTCAAGCGGCGAGTAGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGt                        >  2:1191629/1‑150 (MQ=255)
                                                                                                                           tAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCgg                                                        >  2:1078085/1‑116 (MQ=255)
                                                                                                                           tAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCgg                                                        <  1:1078085/116‑1 (MQ=255)
                                                                                                                                tACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGt                                                                                          <  2:55185/77‑1 (MQ=255)
                                                                                                                                tACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGt                                                                                          >  1:55185/1‑77 (MQ=255)
                                                                                                                                catcgagaaCCGGGCAGCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAacac                 >  2:601108/8‑150 (MQ=255)
                                                                                                                                  cAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAg                          >  1:20290/1‑139 (MQ=255)
                                                                                                                                  cAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAg                          <  2:20290/139‑1 (MQ=255)
                                                                                                                                   aaaaaaCCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTGACGTGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATg              >  2:945127/1‑150 (MQ=255)
                                                                                                                                     aaaaCCCGGCATCGAAGCCAGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTgg                               <  1:2024402/131‑1 (MQ=255)
                                                                                                                                     aaaaCCCGGCATCGAAGCCAGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTgg                               >  2:2024402/1‑131 (MQ=255)
                                                                                                                                       aaCCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGTTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCa          >  1:561707/1‑150 (MQ=255)
                                                                                                                                        aCCCGGCTTCGATGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAAGTTGAACACATGGCCAg         >  1:102015/1‑150 (MQ=255)
                                                                                                                                        aCCCGGCTTCGATGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTAATGCAGGCACCCGCATGTTCCGGGTCAACTTCCCGCGTCATGCCGTGATCAGGGGGGAACACATGTccgg         >  1:1730903/1‑148 (MQ=255)
                                                                                                                                        aCCCGGCTTCGATGCCGGGTTTACTGTGCAGTGCATCAAGTCACGGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCAg         >  2:31244/1‑150 (MQ=255)
                                                                                                                                         cccGGCATCGAAGCCGGGTTTACTGTGCAGTGCCTACCGTTCTTGCCGTCACTGTTCCTACAGTTCGTGCACCGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCAGt        <  1:1142592/150‑1 (MQ=255)
                                                                                                                                          ccGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCAGTg       <  1:649547/150‑1 (MQ=255)
                                                                                                                                               aTCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTGACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCGGCGTGTTCCGGGTCAGCTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCAGTGCCGTg  >  1:748621/1‑150 (MQ=255)
                                                                                                                                               aTCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCATGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCAGTGCCGTg  <  1:1168452/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
CGCAAATGCGCCAGTGCTGAACAAGGGGGCGAGGATGAGGGCGAGATAACGGATTTTCATGATAATCGGGTCCTGTTTCGGTTTCGATGGGACCAGATTAGCGACGATCGCTGAATTCAAACTTAACGTACAAAAAACCCGGCATCGAAGCCGGGTTTACTGTGCAGTGCATCAAGTCACTGGTGGTACTGCGCCGACAGTTCGTGCACGGCGTTGATGAATGCACCCGCATGTTCCGGGTCAACTTCCGGCGTGATGCCGTGGCCAAGGTTGAACACATGGCCAGTGCCGTG  >  NC_002947/5790533‑5790825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑