Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,798,249 C→T 10.1% intergenic (‑294/+80) gltB ← / ← PP_5077 L‑glutamate synthase subunit alpha/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,798,2490CT10.1% 181.0 / 10.2 69intergenic (‑294/+80)gltB/PP_5077L‑glutamate synthase subunit alpha/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/30);  new base T (3/4);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

TTTCATGTCTCGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTG  >  NC_002947/5798100‑5798394
                                                                                                                                                     |                                                                                                                                                 
tttCATGTCTCGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGcc                                                                                                                                                   <  1:1203441/150‑1 (MQ=255)
      gTCTCGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCGGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTc                                                                                                                                             >  2:571174/1‑150 (MQ=255)
      gTCTCGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTc                                                                                                                                             <  2:1140977/150‑1 (MQ=255)
          cGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGgc                                                                                                                                         >  2:470092/1‑150 (MQ=255)
          cGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGgc                                                                                                                                         <  1:1828742/150‑1 (MQ=255)
              aTTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcag                                                                                                                                     <  2:1787710/150‑1 (MQ=255)
                tGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCgcggca                                                                                                                                   <  1:624237/150‑1 (MQ=255)
                 gAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGcggcag                                                                                                                                  <  1:1937760/150‑1 (MQ=255)
                  aaaaTTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGCGGCAgg                                                                                                                                 <  1:686714/150‑1 (MQ=255)
                                          ttttttGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTTAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGACGCGCTCGCGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCtt                                                                                                         <  2:153301/149‑1 (MQ=255)
                                           gttttGCCCCATGCAAAATGATAGCGGCTTGGCTCGAGTCTGAAACGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTa                                                                                                        <  2:1109681/149‑1 (MQ=255)
                                                  gcCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGGCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTg                                                                                                 <  2:688296/150‑1 (MQ=255)
                                                   ccATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTTTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTgg                                                                                                <  1:1149228/150‑1 (MQ=255)
                                                    cATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTggg                                                                                               >  1:1043921/1‑150 (MQ=255)
                                                         gcCGTGATAGCGGCTTGGCTCGAGTTTGAACCGCTTCAGCCATGACTACAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTTTAATTCGCTTAGCTACTGGgcggcg                                                                                          >  2:835530/1‑150 (MQ=255)
                                                         gcCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGcggcag                                                                                                                                  >  2:2259367/1‑110 (MQ=255)
                                                         gcCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGcggcag                                                                                                                                  <  1:2259367/110‑1 (MQ=255)
                                                         gcCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGgcggcg                                                                                          >  1:1648759/1‑150 (MQ=255)
                                                          ccGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGcggcgg                                                                                         <  2:431118/150‑1 (MQ=255)
                                                                tAGCTGTTTCGCTCGAGTCTGCATTGTTTCACTCATGCCTGCAACTAAGGGCTTGTGGCGGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGTTGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGAt                                                                                   <  2:1070545/150‑1 (MQ=255)
                                                                          gCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGc                                                                                                                                <  1:963941/95‑1 (MQ=255)
                                                                          gCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGc                                                                                                                                >  2:963941/1‑95 (MQ=255)
                                                                              gAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCGGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCACTAATTTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGt                                                                     <  1:571174/150‑1 (MQ=255)
                                                                                     aaGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTAtgcggg                                                              >  2:1151497/1‑145 (MQ=255)
                                                                                     aaGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCg                                                              >  2:2306846/1‑150 (MQ=255)
                                                                                           ttCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCggg                                                                                                                                          <  2:1621843/68‑1 (MQ=255)
                                                                                           ttCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCggg                                                                                                                                          >  1:1621843/1‑68 (MQ=255)
                                                                                                ccATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                   >  2:1081879/1‑70 (MQ=255)
                                                                                                ccATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                   <  1:1081879/70‑1 (MQ=255)
                                                                                                ccATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCa                                                   >  2:316610/1‑150 (MQ=255)
                                                                                                  aTGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGaa                                                                        >  2:2200654/1‑127 (MQ=255)
                                                                                                  aTGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGaa                                                                        <  1:2200654/127‑1 (MQ=255)
                                                                                                  aTGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCgg                                                             <  2:2050458/138‑1 (MQ=255)
                                                                                                  aTGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCgg                                                             >  1:2050458/1‑138 (MQ=255)
                                                                                                       tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGaa                                                                                                                >  2:945526/1‑82 (MQ=255)
                                                                                                       tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGaa                                                                                                                <  1:945526/82‑1 (MQ=255)
                                                                                                       tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttg                                            >  2:1108376/1‑150 (MQ=255)
                                                                                                       tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttg                                            <  1:1384768/150‑1 (MQ=255)
                                                                                                        gCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTa                                                                                                                   <  1:2271469/78‑1 (MQ=255)
                                                                                                        gCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTa                                                                                                                   >  2:2271469/1‑78 (MQ=255)
                                                                                                        gCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttgt                                           >  2:17138/1‑150 (MQ=255)
                                                                                                          aaCTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGTCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttgttg                                         >  2:1393598/1‑150 (MQ=255)
                                                                                                           aCTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTgg                                        >  2:445872/1‑150 (MQ=255)
                                                                                                           aCTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTgg                                        <  1:316610/150‑1 (MQ=255)
                                                                                                            cTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGa                                       <  1:17138/150‑1 (MQ=255)
                                                                                                               aGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGGGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCAGTGGCCAGCTCTTGTTTGACGc                                    >  2:124390/1‑150 (MQ=255)
                                                                                                                 ggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                   <  1:1261631/53‑1 (MQ=255)
                                                                                                                 ggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                   >  2:1261631/1‑53 (MQ=255)
                                                                                                                 ggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTg                                  <  2:1043921/150‑1 (MQ=255)
                                                                                                                    ttGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCACTCGGGCGCAGCATGCTAGTCGAAAGCTAGAATACACTTCGCTACTCCTCCCCGGGGCGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGGTTGACGCTGCCg                               >  1:172755/1‑150 (MQ=255)
                                                                                                                     tgtgGGCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCGCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGNAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGa                              <  1:2080104/150‑1 (MQ=255)
                                                                                                                         gCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                   >  2:1200573/1‑45 (MQ=255)
                                                                                                                         gCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                   <  1:1200573/45‑1 (MQ=255)
                                                                                                                         gCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGt                          >  1:1449004/1‑150 (MQ=255)
                                                                                                                          ccGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTa                         >  1:1055305/1‑150 (MQ=255)
                                                                                                                           cGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                        >  1:552797/1‑86 (MQ=255)
                                                                                                                           cGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                        <  2:552797/86‑1 (MQ=255)
                                                                                                                           cGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTAc                        >  2:2326982/1‑150 (MQ=255)
                                                                                                                            ggCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACg                       >  2:2223850/1‑150 (MQ=255)
                                                                                                                                 tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGa                              >  2:2097791/1‑138 (MQ=255)
                                                                                                                                 tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGa                              <  1:2097791/138‑1 (MQ=255)
                                                                                                                                 tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGcc                  >  2:92096/1‑150 (MQ=255)
                                                                                                                                     ggACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAgg              <  1:2305075/150‑1 (MQ=255)
                                                                                                                                          gactaGCCTGCTGCGCCCGAGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGAGGCTGCCGACGGTACGTGGCCAAGGTTTaa         >  1:267833/5‑149 (MQ=255)
                                                                                                                                           actaGCCTGCTGCGCCCGAGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCCGCAGGATGATGCGAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTAcc        >  2:1872902/4‑150 (MQ=255)
                                                                                                                                            aaaGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTa                                                                                                                   <  2:647090/42‑1 (MQ=255)
                                                                                                                                            aaaGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTa                                                                                                                   >  1:647090/1‑42 (MQ=255)
                                                                                                                                             aaGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAg                                                  >  1:122240/1‑106 (MQ=255)
                                                                                                                                             aaGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAg                                                  <  2:122240/106‑1 (MQ=255)
                                                                                                                                                 cTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTg  <  1:1108376/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTTCATGTCTCGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTG  >  NC_002947/5798100‑5798394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑