Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,798,257	G→A	10.4%	intergenic (‑302/+72)	gltB ← / ← PP_5077	L‑glutamate synthase subunit alpha/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,798,257	0	G	A	10.4%	177.0 / 9.9	68	intergenic (‑302/+72)	gltB/PP_5077	L‑glutamate synthase subunit alpha/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/28);  new base A (3/4);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTGGACCTTCGCTG  >  NC_002947/5798110‑5798405                                                                                                                                                    |                                                                                                                                                     cGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGgc                                                                                                                                                    <  1:1828742/150‑1 (MQ=255) cGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGgc                                                                                                                                                    >  2:470092/1‑150 (MQ=255)     aTTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcag                                                                                                                                                <  2:1787710/150‑1 (MQ=255)       tGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCgcggca                                                                                                                                              <  1:624237/150‑1 (MQ=255)        gAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGcggcag                                                                                                                                             <  1:1937760/150‑1 (MQ=255)         aaaaTTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGCGGCAgg                                                                                                                                            <  1:686714/150‑1 (MQ=255)                                 ttttttGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTTAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGACGCGCTCGCGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCtt                                                                                                                    <  2:153301/149‑1 (MQ=255)                                  gttttGCCCCATGCAAAATGATAGCGGCTTGGCTCGAGTCTGAAACGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTa                                                                                                                   <  2:1109681/149‑1 (MQ=255)                                         gcCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGGCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTg                                                                                                            <  2:688296/150‑1 (MQ=255)                                          ccATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTTTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTgg                                                                                                           <  1:1149228/150‑1 (MQ=255)                                           cATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTggg                                                                                                          >  1:1043921/1‑150 (MQ=255)                                                gcCGTGATAGCGGCTTGGCTCGAGTTTGAACCGCTTCAGCCATGACTACAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTTTAATTCGCTTAGCTACTGGgcggcg                                                                                                     >  2:835530/1‑150 (MQ=255)                                                gcCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGcggcag                                                                                                                                             >  2:2259367/1‑110 (MQ=255)                                                gcCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGcggcag                                                                                                                                             <  1:2259367/110‑1 (MQ=255)                                                gcCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGgcggcg                                                                                                     >  1:1648759/1‑150 (MQ=255)                                                 ccGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGcggcgg                                                                                                    <  2:431118/150‑1 (MQ=255)                                                       tAGCTGTTTCGCTCGAGTCTGCATTGTTTCACTCATGCCTGCAACTAAGGGCTTGTGGCGGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGTTGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGAt                                                                                              <  2:1070545/150‑1 (MQ=255)                                                                 gCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGc                                                                                                                                           <  1:963941/95‑1 (MQ=255)                                                                 gCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGc                                                                                                                                           >  2:963941/1‑95 (MQ=255)                                                                     gAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCGGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCACTAATTTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGt                                                                                <  1:571174/150‑1 (MQ=255)                                                                            aaGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTAtgcggg                                                                         >  2:1151497/1‑145 (MQ=255)                                                                            aaGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCg                                                                         >  2:2306846/1‑150 (MQ=255)                                                                                  ttCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCggg                                                                                                                                                     <  2:1621843/68‑1 (MQ=255)                                                                                  ttCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCggg                                                                                                                                                     >  1:1621843/1‑68 (MQ=255)                                                                                       ccATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                              >  2:1081879/1‑70 (MQ=255)                                                                                       ccATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                              <  1:1081879/70‑1 (MQ=255)                                                                                       ccATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCa                                                              >  2:316610/1‑150 (MQ=255)                                                                                         aTGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGaa                                                                                   <  1:2200654/127‑1 (MQ=255)                                                                                         aTGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGaa                                                                                   >  2:2200654/1‑127 (MQ=255)                                                                                         aTGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCgg                                                                        >  1:2050458/1‑138 (MQ=255)                                                                                         aTGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCgg                                                                        <  2:2050458/138‑1 (MQ=255)                                                                                              tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGaa                                                                                                                           >  2:945526/1‑82 (MQ=255)                                                                                              tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGaa                                                                                                                           <  1:945526/82‑1 (MQ=255)                                                                                              tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttg                                                       >  2:1108376/1‑150 (MQ=255)                                                                                              tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttg                                                       <  1:1384768/150‑1 (MQ=255)                                                                                               gCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTa                                                                                                                              <  1:2271469/78‑1 (MQ=255)                                                                                               gCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTa                                                                                                                              >  2:2271469/1‑78 (MQ=255)                                                                                               gCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttgt                                                      >  2:17138/1‑150 (MQ=255)                                                                                                 aaCTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGTCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttgttg                                                    >  2:1393598/1‑150 (MQ=255)                                                                                                  aCTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTgg                                                   >  2:445872/1‑150 (MQ=255)                                                                                                  aCTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTgg                                                   <  1:316610/150‑1 (MQ=255)                                                                                                   cTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGa                                                  <  1:17138/150‑1 (MQ=255)                                                                                                      aGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGGGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCAGTGGCCAGCTCTTGTTTGACGc                                               >  2:124390/1‑150 (MQ=255)                                                                                                        ggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                              <  1:1261631/53‑1 (MQ=255)                                                                                                        ggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                              >  2:1261631/1‑53 (MQ=255)                                                                                                        ggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTg                                             <  2:1043921/150‑1 (MQ=255)                                                                                                           ttGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCACTCGGGCGCAGCATGCTAGTCGAAAGCTAGAATACACTTCGCTACTCCTCCCCGGGGCGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGGTTGACGCTGCCg                                          >  1:172755/1‑150 (MQ=255)                                                                                                            tgtgGGCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCGCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGNAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGa                                         <  1:2080104/150‑1 (MQ=255)                                                                                                                gCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                              >  2:1200573/1‑45 (MQ=255)                                                                                                                gCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                              <  1:1200573/45‑1 (MQ=255)                                                                                                                gCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGt                                     >  1:1449004/1‑150 (MQ=255)                                                                                                                 ccGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTa                                    >  1:1055305/1‑150 (MQ=255)                                                                                                                  cGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                                   <  2:552797/86‑1 (MQ=255)                                                                                                                  cGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                                   >  1:552797/1‑86 (MQ=255)                                                                                                                  cGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTAc                                   >  2:2326982/1‑150 (MQ=255)                                                                                                                   ggCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACg                                  >  2:2223850/1‑150 (MQ=255)                                                                                                                        tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGa                                         >  2:2097791/1‑138 (MQ=255)                                                                                                                        tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGa                                         <  1:2097791/138‑1 (MQ=255)                                                                                                                        tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGcc                             >  2:92096/1‑150 (MQ=255)                                                                                                                            ggACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAgg                         <  1:2305075/150‑1 (MQ=255)                                                                                                                                 gactaGCCTGCTGCGCCCGAGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGAGGCTGCCGACGGTACGTGGCCAAGGTTTaa                    >  1:267833/5‑149 (MQ=255)                                                                                                                                  actaGCCTGCTGCGCCCGAGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCCGCAGGATGATGCGAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTAcc                   >  2:1872902/4‑150 (MQ=255)                                                                                                                                   aaaGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTa                                                                                                                              >  1:647090/1‑42 (MQ=255)                                                                                                                                   aaaGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTa                                                                                                                              <  2:647090/42‑1 (MQ=255)                                                                                                                                    aaGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAg                                                             >  1:122240/1‑106 (MQ=255)                                                                                                                                    aaGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAg                                                             <  2:122240/106‑1 (MQ=255)                                                                                                                                        cTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTg             <  1:1108376/150‑1 (MQ=255)                                                                                                                                                 tCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGc                                                                      <  2:2265951/84‑1 (MQ=255)                                                                                                                                                 tCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGc                                                                      >  1:2265951/1‑84 (MQ=255)                                                                                                                                                 tCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTGGACCTTCGc    >  1:1824212/1‑150 (MQ=255)                                                                                                                                                   gggCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTGGACCTTCGCTg  <  1:1393598/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTGGACCTTCGCTG  >  NC_002947/5798110‑5798405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑