BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	535,822	A→C	9.1%	E85A (GAA→GCA) ‡	rplA →	50S ribosomal protein L1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	535,822	0	A	C	9.1%	276.8 / 17.2	99	E85A (GAA→GCA) ‡	rplA	50S ribosomal protein L1
Reads supporting (aligned to +/- strand): ref base A (39/51); new base C (5/4); total (44/55)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

Only 100 of 101 total aligned reads displayed.

CCAAGTTCGTCGAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTGGGTCCTCGC  >  NC_002947/535675‑535970

ccAAGTTCGTCGAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAg                                                                                                                                                    >  2:2075359/1‑150 (MQ=255)

ccAAGTTCGTCGAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGCGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAg                                                                                                                                                    <  2:1763111/150‑1 (MQ=255)

 cAAGTTCGTCGAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAgc                                                                                                                                                   >  2:539295/1‑150 (MQ=255)

     ttCGTCGAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAgctgct                                                                                                                                               >  1:1713829/1‑150 (MQ=255)

      tcgtcgAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTTCAgctgctg                                                                                                                                              <  1:938828/150‑2 (MQ=255)

      tcgtcgAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTTCAgctgctg                                                                                                                                              <  1:1246504/150‑2 (MQ=255)

         tcgAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTgg                                                                                                                                           <  1:2240371/150‑1 (MQ=255)

           gAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCt                                                                                                                                         >  1:766689/1‑150 (MQ=255)

           gAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCt                                                                                                                                         <  2:53171/150‑1 (MQ=255)

                cTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCgg                                                                                                                                    >  1:1646284/1‑150 (MQ=255)

                cTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCgg                                                                                                                                    >  2:889916/1‑150 (MQ=255)

                cTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCgg                                                                                                                                    >  2:2335406/1‑150 (MQ=255)

                 tACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGc                                                                                                                                       >  2:1407671/1‑146 (MQ=255)

                 tACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGc                                                                                                                                       <  1:1407671/146‑1 (MQ=255)

                 tACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGgc                                                                                                                                   >  1:187061/1‑150 (MQ=255)

                   cGACATCGCCGTTAACCTCGTTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGgcgc                                                                                                                                 <  1:1827953/150‑1 (MQ=255)

                       aTCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACATGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGAt                                                                                                                             <  1:2289734/150‑1 (MQ=255)

                           ccGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATcgcg                                                                                                                         >  1:896039/1‑150 (MQ=255)

                             gTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTa                                                                                                                       <  1:2258963/150‑1 (MQ=255)

                             gTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTa                                                                                                                       <  1:2075359/150‑1 (MQ=255)

                              ttAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAg                                                                                                                      <  2:1575167/150‑1 (MQ=255)

                              ttAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAg                                                                                                                      <  1:1004680/150‑1 (MQ=255)

                                aaCCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGt                                                                                                                    <  2:2257930/150‑1 (MQ=255)

                                     cGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGa                                                                                                               <  2:766689/150‑1 (MQ=255)

                                          ttGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATc                                                                                                          <  2:1469616/150‑1 (MQ=255)

                                          ttGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATc                                                                                                          <  2:1743213/150‑1 (MQ=255)

                                          ttGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATc                                                                                                          >  1:1007329/1‑150 (MQ=255)

                                            gACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTg                                                                                                        <  1:7358/150‑1 (MQ=255)

                                             aCCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCGGACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATc                                                                                                          <  1:1123740/147‑1 (MQ=255)

                                             aCCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATc                                                                                                          >  2:1123740/1‑147 (MQ=255)

                                               ccGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGCATGGACGATCTGGCt                                                                                                     >  2:1675097/1‑150 (MQ=255)

                                                cgcgTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATTTACGCgctggctg                                                                                                    >  2:1869299/1‑150 (MQ=255)

                                                 gcgTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGc                                                                                                   >  1:2249686/1‑150 (MQ=255)

                                                   gTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATc                                                                                                          >  1:144303/1‑141 (MQ=255)

                                                   gTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATc                                                                                                          <  2:144303/141‑1 (MQ=255)

                                                      aaTCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGaaa                                                                                              <  2:1646284/150‑1 (MQ=255)

                                                           gACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGaaa                                                                                         <  1:393911/150‑1 (MQ=255)

                                                           gACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACCGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGaaa                                                                                         <  1:539295/150‑1 (MQ=255)

                                                              cAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAggc                                                                                      <  2:1907115/150‑1 (MQ=255)

                                                                ggTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAggcgg                                                                                    <  2:896039/150‑1 (MQ=255)

                                                                    gtacgtaGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGAc                                                                                <  2:574415/150‑1 (MQ=255)

                                                                     tacgtaGCGATACCGTCGTCCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGAcc                                                                               <  2:656358/150‑1 (MQ=255)

                                                                      acgtaGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGa                                                                                                               >  2:404255/1‑117 (MQ=255)

                                                                      acgtaGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGa                                                                                                               <  1:404255/117‑1 (MQ=255)

                                                                        gtaGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGa                                                                                                            >  1:2016632/1‑118 (MQ=255)

                                                                        gtaGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGa                                                                                                            <  2:2016632/118‑1 (MQ=255)

                                                                        gtaGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGa                                                                            >  1:1550638/1‑150 (MQ=255)

                                                                         taGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGaa                                                                           <  2:2289525/150‑1 (MQ=255)

                                                                          aGCGGTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAAc                                                                          <  2:808880/150‑1 (MQ=255)

                                                                          aGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAAc                                                                          <  2:2271307/150‑1 (MQ=255)

                                                                             gcTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTAt                                                                       >  2:1864735/1‑150 (MQ=255)

                                                                             gcTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTAt                                                                       <  1:1718751/150‑1 (MQ=255)

                                                                             gcTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTAt                                                                       <  1:1864735/150‑1 (MQ=255)

                                                                             gcTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTAt                                                                       <  1:2335406/150‑1 (MQ=255)

                                                                               tACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGa                                                                     >  1:812572/1‑150 (MQ=255)

                                                                               tACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGa                                                                     <  2:1951443/150‑1 (MQ=255)

                                                                               tACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGa                                                                     >  2:46659/1‑150 (MQ=255)

                                                                                         ccACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTatc                                                           >  2:177737/1‑150 (MQ=255)

                                                                                           acacGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGAACTGAACTATGACGGCGTGatccc                                                         >  2:458719/1‑148 (MQ=255)

                                                                                             acGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTatcgcat                                                       >  1:2370218/1‑150 (MQ=255)

                                                                                                           aCCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCNGCCNTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTGTGACGTCGTTGTCGCATCGCCTGATGCCATg                                         >  1:1930695/1‑150 (MQ=255)

                                                                                                               ttCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGAc                                                                    <  2:1332247/119‑1 (MQ=255)

                                                                                                               ttCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGAc                                                                    >  1:1332247/1‑119 (MQ=255)

                                                                                                                tCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGt                                    <  2:1713829/150‑1 (MQ=255)

                                                                                                                   cgTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCt                                                                                                                                         >  1:1181224/1‑46 (MQ=255)

                                                                                                                   cgTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCt                                                                                                                                         <  2:1181224/46‑1 (MQ=255)

                                                                                                                   cgTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGt                                 <  1:458719/150‑1 (MQ=255)

                                                                                                                     ttGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAg                               <  2:1279368/150‑1 (MQ=255)

                                                                                                                       gCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGt                             >  1:938857/1‑150 (MQ=255)

                                                                                                                        cTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGttcgcggtc                            >  2:1101680/1‑143 (MQ=255)

                                                                                                                           tCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGAc                                                                                >  2:2303927/1‑95 (MQ=255)

                                                                                                                           tCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGCAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGAc                                                                                <  1:2303927/95‑1 (MQ=255)

                                                                                                                              tCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTgg                      <  1:177737/150‑1 (MQ=255)

                                                                                                                              tCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTgg                      <  2:1007329/150‑1 (MQ=255)

                                                                                                                                aCCCAGGGTCCAGCAGCTGAAGATGCTCTGGCTGCCAGCGCTGATCGCGAACGTATGGACGAGCTTGCAGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCAAACGCGTTGTAGGTCAGCTgggg                    >  2:142135/1‑149 (MQ=255)

                                                                                                                                 cccAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTc                   >  2:896746/1‑150 (MQ=255)

                                                                                                                                   cAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAATGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTa                                >  1:497772/1‑135 (MQ=255)

                                                                                                                                   cAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTa                                <  2:497772/135‑1 (MQ=255)

                                                                                                                                   cAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAg                 >  2:1579308/1‑150 (MQ=255)

                                                                                                                                   cAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAg                 <  2:1930695/150‑1 (MQ=255)

                                                                                                                                       ccagagcagcTTCAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTc             >  1:924689/5‑150 (MQ=255)

                                                                                                                                       ccagagcagcTTCAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTc             >  1:745088/5‑150 (MQ=255)

                                                                                                                                         agagcagcTTCAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTg           >  2:1868728/3‑150 (MQ=255)

                                                                                                                                         agagcagcTTCAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTg           >  1:1731910/3‑150 (MQ=255)

                                                                                                                                         agagcagcTTCAGCTGCTCTGGCTGCCGGCGCTGATCCCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTg           >  2:1177144/3‑150 (MQ=255)

                                                                                                                                          gagcagcTTCAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTgg          <  2:1731910/149‑1 (MQ=255)

                                                                                                                                          cagcagCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTatc                                                           <  1:834227/101‑1 (MQ=255)

                                                                                                                                          cagcagCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTatc                                                           >  2:834227/1‑101 (MQ=255)

                                                                                                                                          cagcagCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTgg          <  1:889916/150‑1 (MQ=255)

                                                                                                                                          cagcagCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTgg          <  1:1579308/150‑1 (MQ=255)

                                                                                                                                          cagcagCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTgg          <  2:187061/150‑1 (MQ=255)

                                                                                                                                          cagcagCGGAAGATGCTCTGGCGGGCGGCGCTGATCGCGTAGGTATGGAAGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTgg          <  1:1101680/150‑1 (MQ=255)

                                                                                                                                           agcagcTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTatcgc                                                         <  1:734743/102‑1 (MQ=255)

                                                                                                                                           agcagcTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTatcgc                                                         >  2:734743/1‑102 (MQ=255)

                                                                                                                                           agcagcTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTCACTCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTggg         <  1:1869299/150‑1 (MQ=255)

                                                                                                                                             cagcTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATTCGCGTTGTAGGTCAGCTGGGTCAGGTTCTGGGTc       <  1:424212/150‑1 (MQ=255)

                                                                                                                                              agcTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTGGGTcc      >  2:1960890/1‑150 (MQ=255)

                                                                                                                                                  gAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTGGGTCCTcgc  >  1:1594285/1‑150 (MQ=255)

                                                                                                                                                   aaGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTgg          <  1:2056657/141‑1 (MQ=255)

                                                                                                                                                   aaGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTgg          >  2:2056657/1‑141 (MQ=255)

CCAAGTTCGTCGAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTGGGTCCTCGC  >  NC_002947/535675‑535970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`