Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,828,023 C→G 5.9% intergenic (+169/+30) mtgA → / ← rpoH monofunctional biosynthetic peptidoglycan transglycosylase/RNA polymerase factor sigma‑32

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,828,0230CG 5.9% 499.7 / 16.1 153intergenic (+169/+30)mtgA/rpoHmonofunctional biosynthetic peptidoglycan transglycosylase/RNA polymerase factor sigma‑32
Reads supporting (aligned to +/- strand):  ref base C (74/70);  new base G (3/6);  total (77/76)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 157 total aligned reads displayed.

GGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAGCGTGGCCTTCTCTTCCGCCAG  >  NC_002947/5827876‑5828172
                                                                                                                                                   |                                                                                                                                                     
gggTAAACCCGCTCCTACGGGAAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAg                                                                                                                                                     >  1:1493336/1‑150 (MQ=255)
 ggTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGc                                                                                                                                                    >  2:1015968/1‑150 (MQ=255)
 ggTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCACCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGAGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGc                                                                                                                                                    <  2:764866/150‑1 (MQ=255)
       cccGCTCGTACGGCCAACCGCATACCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGAAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGTCGGCAAAGCCAAACATAAAAGCCGCTGGCCCTATGGGGTGAGCGGCttt                                                                                                                                              <  2:896741/150‑1 (MQ=255)
       cccGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCttt                                                                                                                                              >  1:1439981/1‑150 (MQ=255)
        ccGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCtttt                                                                                                                                             >  2:1001304/1‑150 (MQ=255)
        ccGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCtttt                                                                                                                                             >  2:1655911/1‑150 (MQ=255)
           cTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCtt                                                                                                                                          <  1:2377660/150‑1 (MQ=255)
              cTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCTGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTGGCCCTATGGGGTGAGCGGCttttttgttt                                                                                                                                       <  1:291552/150‑7 (MQ=37)
              cTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAGAAAAGCCGCTGGCCCTATGGGGTGAGCGGCttttttgttt                                                                                                                                       <  1:1517162/150‑7 (MQ=37)
                aCGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACaa                                                                                                                                     >  2:11468/1‑150 (MQ=255)
                  gggCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAAcc                                                                                                                                   >  2:1020773/1‑150 (MQ=255)
                    gCAACCGCATCCCCGGCCTCCTCGCGGGCCAAACACCGGCTGGCCATAACCTCAGCCCCTGTAGGAGCGGGAAACCTCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCa                                                                                                                                 <  1:1739473/150‑1 (MQ=255)
                    gCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCa                                                                                                                                 >  2:1176032/1‑150 (MQ=255)
                        ccGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGTCGACCTCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACt                                                                                                                             >  2:2269360/1‑150 (MQ=255)
                            gtCCGCGGCGTCCGCGCGGGCGAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAAt                                                                                                                         <  2:2188120/149‑1 (MQ=255)
                             tCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATc                                                                                                                        <  2:2344992/150‑1 (MQ=255)
                                ccGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCAGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAgg                                                                                                                     >  1:758360/1‑150 (MQ=255)
                                 cGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGc                                                                                                                    >  1:481378/1‑150 (MQ=255)
                                     ctcctcGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTc                                                                                                                                            <  2:945388/122‑1 (MQ=255)
                                      tcctcGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAgg                                                                                                                     >  2:29562/1‑144 (MQ=255)
                                      tcctcGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAgg                                                                                                                     <  1:29562/144‑1 (MQ=255)
                                      tcctcGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGa                                                                                                               >  1:340725/1‑150 (MQ=255)
                                      tcctcGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGa                                                                                                               >  1:2058368/1‑150 (MQ=255)
                                         tcGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCaa                                                                                                                          >  1:163958/1‑136 (MQ=255)
                                         tcGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCaa                                                                                                                          <  2:163958/136‑1 (MQ=255)
                                         tcGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCa                                                                                                            <  2:758360/150‑1 (MQ=255)
                                            cgGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTg                                                                                                         <  1:1015968/150‑1 (MQ=255)
                                            cgGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTg                                                                                                         <  1:1020773/150‑1 (MQ=255)
                                                     ccGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTa                                                                                                                                        <  1:1452693/110‑1 (MQ=255)
                                                     ccGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTa                                                                                                                                        >  2:1452693/1‑110 (MQ=255)
                                                     ccGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCt                                                                                                >  1:1507857/1‑150 (MQ=255)
                                                        cgcCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGt                                                                                             <  1:1001304/150‑1 (MQ=255)
                                                        cgcCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGt                                                                                             <  2:1552695/150‑1 (MQ=255)
                                                          ccTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTc                                                                                           >  1:1240782/1‑150 (MQ=255)
                                                          ccTACAGGCAACCGCAGCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTc                                                                                           <  2:2341995/150‑1 (MQ=255)
                                                             aCAGGCAACCGCATCCGCTGTAGGAGCGTGCTTGCCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATc                                                                                        <  1:2054126/150‑1 (MQ=255)
                                                             aCAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATc                                                                                        <  2:981057/150‑1 (MQ=255)
                                                              cAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGACGAGCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCg                                                                                       >  2:2131660/1‑150 (MQ=255)
                                                               aGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAACCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCGAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGc                                                                                      >  2:1737653/1‑150 (MQ=255)
                                                                 gCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCAt                                                                                    >  1:641149/1‑150 (MQ=255)
                                                                   aaCCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCAttc                                                                                  <  1:95102/150‑1 (MQ=255)
                                                                   aaCCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAAACCAGACTCAATCAGGCAGAGACCAGTGCCTTGACCTTGTTCACCGCCttc                                                                                  >  1:1704380/1‑150 (MQ=255)
                                                                      cgaaTCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAGGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCAttcttc                                                                               >  1:398706/4‑150 (MQ=255)
                                                                       gCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATtcttct                                                                              <  2:2380858/150‑1 (MQ=255)
                                                                            cccTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAAc                                                                         <  2:641149/150‑1 (MQ=255)
                                                                            cccTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAAc                                                                         <  1:11468/150‑1 (MQ=255)
                                                                             ccTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTa                                                                                                                                        >  2:1389048/1‑86 (MQ=255)
                                                                             ccTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTtctc                                                                             <  2:69047/145‑1 (MQ=255)
                                                                             ccTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTtctc                                                                             >  1:69047/1‑145 (MQ=255)
                                                                              cTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTg                                                                       >  2:107488/1‑150 (MQ=255)
                                                                                gTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGc                                                                     <  1:1898190/150‑1 (MQ=255)
                                                                                gTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGc                                                                     <  1:1656054/150‑1 (MQ=255)
                                                                                    gAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAAt                                                                 >  1:1067328/1‑150 (MQ=255)
                                                                                         ggCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCt                                                            >  1:1661503/1‑150 (MQ=255)
                                                                                          gtttACCCGCGAAAGGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCt                                                            >  1:404833/3‑149 (MQ=255)
                                                                                                ccGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGaa                                                                  <  1:1631884/137‑1 (MQ=255)
                                                                                                ccGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGa                                                     >  1:2173398/1‑150 (MQ=255)
                                                                                                     aaGAGGCCGGCAAAGCCAAAAAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGc                                                <  1:2170254/150‑1 (MQ=255)
                                                                                                       gagGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTg                                              <  1:1176032/150‑1 (MQ=255)
                                                                                                         ggCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTa                                            <  1:1228511/150‑1 (MQ=255)
                                                                                                            cGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTAc                                           <  1:712170/148‑1 (MQ=255)
                                                                                                             ggCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTg                                        >  2:1296866/1‑150 (MQ=255)
                                                                                                              gCAAAGCCAAACAAAAAAGCCTCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGACTACGCCCAGCCGCTACGCTGTACTTGt                                       <  2:1240782/150‑1 (MQ=255)
                                                                                                               cAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTc                                      <  2:369912/150‑1 (MQ=255)
                                                                                                               cAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTc                                      >  1:610355/1‑150 (MQ=255)
                                                                                                                aaaGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCt                                     >  1:729936/1‑150 (MQ=255)
                                                                                                                  aGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGc                                   >  2:1104481/1‑150 (MQ=255)
                                                                                                                   gCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATc                                                                                                                        >  2:433262/1‑64 (MQ=255)
                                                                                                                   gCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGa                                                                                                   <  2:1617152/85‑1 (MQ=255)
                                                                                                                   gCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGa                                                                                                   >  1:1617152/1‑85 (MQ=255)
                                                                                                                    ccAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGt                                                                                             <  1:2206258/90‑1 (MQ=255)
                                                                                                                    ccAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCa                                 >  1:2399558/1‑150 (MQ=255)
                                                                                                                    ccAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCa                                 <  2:2173398/150‑1 (MQ=255)
                                                                                                                    ccAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCa                                 >  1:978828/1‑150 (MQ=255)
                                                                                                                     caaacaaaAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACtt                                         <  1:2266073/141‑1 (MQ=255)
                                                                                                                     caaacaaaAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACtt                                         >  2:2266073/1‑141 (MQ=255)
                                                                                                                      aaacaaaAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGc                                                                     >  2:574048/1‑112 (MQ=255)
                                                                                                                      aaacaaaAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGc                                                                     <  1:574048/112‑1 (MQ=255)
                                                                                                                      aaacaaaAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGt                               >  2:177312/1‑150 (MQ=255)
                                                                                                                      aaacaaaAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGt                               >  1:2127879/1‑150 (MQ=255)
                                                                                                                             aaaGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTCCCACCCAGACTCAATCAGCCAGCGACAAGTGCCTTAACCTTGTTCATCGCATTCTTCTCCAACTGCCGAATAAGCTCCGCCGCTACGCTCTACTTGTATGCCAGTTCATGcc                        >  2:680052/1‑149 (MQ=255)
                                                                                                                             aaaGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCa                        >  1:1927530/1‑150 (MQ=255)
                                                                                                                              aaGCCGCTGGCCCTATGGGGTGAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAg                       >  2:739088/1‑150 (MQ=255)
                                                                                                                                gCCGCTAACCCCATGGGGCCATCGGTTTTGCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAGCg                     >  2:1956215/1‑150 (MQ=255)
                                                                                                                                 ccGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTAATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAGCGt                    <  2:2058368/150‑1 (MQ=255)
                                                                                                                                   gCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATa                                                   >  2:2235425/1‑117 (MQ=255)
                                                                                                                                   gCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAGCGTgg                  >  1:1690247/1‑150 (MQ=255)
                                                                                                                                       aCCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGt                                                                                                          >  1:2237929/1‑58 (MQ=255)
                                                                                                                                       aCCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGc                                                             <  2:728453/103‑1 (MQ=255)
                                                                                                                                       aCCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAGCGTGGCCtt              <  2:1555475/150‑1 (MQ=255)
                                                                                                                                       aCCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAGCGTGGCCtt              >  2:2134604/1‑150 (MQ=255)
                                                                                                                                         cccATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAGCGTGGCCTtct            <  2:1712074/150‑1 (MQ=255)
                                                                                                                                         cccATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAGCGTGGCCTtct            >  2:836281/1‑150 (MQ=255)
                                                                                                                                          ccATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGGTACGCTGTACTTGTCTGCCAGTTCATGCAGCGTGGCCTtc             >  1:622961/1‑148 (MQ=255)
                                                                                                                                          ccATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGGTACGCTGTACTTGTCTGCCAGTTCATGCAGCGTGGCCTtc             <  2:622961/148‑1 (MQ=255)
                                                                                                                                              aGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAGCGTGGCCTTCTCTTcc       <  2:2097442/150‑1 (MQ=255)
                                                                                                                                                   cAGCGGCTTTTCTTTACAACCCAGACTCAATGAGGCCGCCATCAGTGCCTTGACCTTGATCATCTCATTCAACGGCAACTGTTCAAGAGGCTCAGCGGATACGCTGGACTTGTCTGCCAGTTCATGCAGCGTGGCCTTCTCTTCCgccag  >  2:1478606/1‑150 (MQ=255)
                                                                                                                                                   cAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATGCGCTGTACTTGTCTGCCAGTTCATGCAGCGTg                   >  1:2108259/1‑133 (MQ=255)
                                                                                                                                                   cAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAGCGTg                   <  2:2108259/133‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGGTAAACCCGCTCCTACGGGCAACCGCATCCCCGGCCTCCTCGCGGGCAAACCCGCGCCTACAGGCAACCGCATCCCCTGTAGGAGCGGGCTTACCCGCGAAGAGGCCGGCAAAGCCAAACAAAAAAGCCGCTCACCCCATAGGGCCAGCGGCTTTTCTTTACAACCCAGACTCAATCAGGCAGCGATCAGTGCCTTGACCTTGTTCATCGCATTCTTCTCCAACTGGCGAATACGCTCAGCCGATACGCTGTACTTGTCTGCCAGTTCATGCAGCGTGGCCTTCTCTTCCGCCAG  >  NC_002947/5827876‑5828172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑